Non-interfacial self-assembly of synthetic protocells

Xiaolin Xu,Wencai Guan,Xiaolei Yu,Guoxiong Xu,Chenglong Wang 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Protocell refers to the basic unit of life and synthetic molecular assembly with cell structure and function. The protocells have great applications in the field of biomedical technology. Simulating the morphology and function of cells is the key to the preparation of protocells. However, some organic solvents used in the preparation process of protocells would damage the function of the bioactive substance. Perfluorocarbon, which has no toxic effect on bioactive substances, is an ideal solvent for protocell preparation. However, perfluorocarbon cannot be emulsified with water because of its inertia. Methods Spheroids can be formed in nature even without emulsification, since liquid can reshape the morphology of the solid phase through the scouring action, even if there is no stable interface between the two phases. Inspired by the formation of natural spheroids such as pebbles, we developed non-interfacial self-assembly (NISA) of microdroplets as a step toward synthetic protocells, in which the inert perfluorocarbon was utilized to reshape the hydrogel through the scouring action. Results The synthetic protocells were successfully obtained by using NISA-based protocell techniques, with the morphology very similar to native cells. Then we simulated the cell transcription process in the synthetic protocell and used the protocell as an mRNA carrier to transfect 293T cells. The results showed that protocells delivered mRNAs, and successfully expressed proteins in 293T cells. Further, we used the NISA method to fabricate an artificial cell by extracting and reassembling the membrane, proteins, and genomes of ovarian cancer cells. The results showed that the recombination of tumor cells was successfully achieved with similar morphology as tumor cells. In addition, the synthetic protocell prepared by the NISA method was used to reverse cancer chemoresistance by restoring cellular calcium homeostasis, which verified the application value of the synthetic protocell as a drug carrier. Conclusion This synthetic protocell fabricated by the NISA method simulates the occurrence and development process of primitive life, which has great potential application value in mRNA vaccine, cancer immunotherapy, and drug delivery.

Density Functional Theory Study on Triphenylamine-based Dye Sensitizers Containing Different Donor Moieties

Xu, Jie,Wang, Lei,Liang, Guijie,Bai, Zikui,Wang, Luoxin,Xu, Weilin,Shen, Xiaolin Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.9

Density functional theory (DFT) and time-dependent DFT (TD-DFT) calculations have been employed to investigate the molecular structures and absorption spectra of two dyes containing diphenylaniline and 4-diphenylamino-diphenylaniline as donor moiety (TPA1 and TPA3). The geometries indicate that the strong conjugation is formed in the dyes. The electronic structures suggest that the intramolecular charge transfer from the donor to the acceptor occurs, and the electron-donating capability of 4-diphenylamino-diphenylaniline is stronger than that of diphenylaniline. The computed highest occupied molecular orbital (HOMO) energy levels are -5.31 and -4.90 eV, while the lowest unoccupied molecular orbital (LUMO) energies are -2.29 and -2.26 eV for TPA1 and TPA3, respectively, revealing that the interfacial charge transfer between the dyes and the semiconductor electrode are electron injection processes from the photon-excited dyes to the semiconductor conduction band. Furthermore, all the experimental absorption bands of TPA1 and TPA3 have been assigned according to the TDDFT calculations.

Study on Evaluation Model of Attribute Barycentric Coordinates

Guanglin Xu,Xiaolin Xu 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.9

The core of the comprehensive evaluation problem is how to distribute the weight of each attribute. The attribute coordinate evaluation model (ACEM) has realized dynamic distribution for weight according to the evaluator preference curve, but there still is a problem that maybe a few evaluation results are unreasonable after transforming from locally satisfactory degree to globally satisfactory degree. In order to solve this problem, this paper proposes the improved attribute coordinate evaluation model (IACEM) which directly calculates the global satisfaction, simultaneously, gives the principle to determine whether the final evaluation result is reasonable or not, namely, the principle of weight priority is adopted for the evaluated objects with the same total score level, and the principle of total score priority is adopted for the evaluated objects with different total score levels. To eliminate the errors caused by the change of total score planes, this paper also proposes a parameter adjustment method. The final simulation verifies the correctness and the rationality of the improved model.

Density Functional Theory Study on Triphenylamine-based Dye Sensitizers Containing Different Donor Moieties

Jie Xu,Lei Wang,Guijie Liang,Zikui Bai,Luoxin Wang,Weilin Xu,Xiaolin Shen 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.9

Density functional theory (DFT) and time-dependent DFT (TD-DFT) calculations have been employed to investigate the molecular structures and absorption spectra of two dyes containing diphenylaniline and 4-diphenylamino-diphenylaniline as donor moiety (TPA1 and TPA3). The geometries indicate that the strong conjugation is formed in the dyes. The electronic structures suggest that the intramolecular charge transfer from the donor to the acceptor occurs, and the electron-donating capability of 4-diphenylamino-diphenylaniline is stronger than that of diphenylaniline. The computed highest occupied molecular orbital (HOMO) energy levels are ‒5.31 and ‒4.90 eV, while the lowest unoccupied molecular orbital (LUMO) energies are ‒2.29 and ‒2.26 eV for TPA1 and TPA3, respectively, revealing that the interfacial charge transfer between the dyes and the semiconductor electrode are electron injection processes from the photonexcited dyes to the semiconductor conduction band. Furthermore, all the experimental absorption bands of TPA1 and TPA3 have been assigned according to the TDDFT calculations.

중국 카이펑시 경관디자인에서 나타난 지속가능성 연구 - 청명상하원 테마파크 중심으로 -

허효림(Xu xiaolin),윤지영(Yoon, Ji-Young) 한국실내디자인학회 2021 한국실내디자인학회 학술대회논문집 Vol.23 No.1

Selection response and estimation of the genetic parameters for multidimensional measured breast meat yield related traits in a long-term breeding Pekin duck line

Xu, Yaxi,Hu, Jian,Zhang, Yunsheng,Guo, Zhanbao,Huang, Wei,Xie, Ming,Liu, Hehe,Lei, Chuzhao,Hou, Shuisheng,Liu, Xiaolin,Zhou, Zhengkui Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.10

Objective: This study was conducted to estimate the genetic parameters and breeding values of breast meat related traits of Pekin ducks. Selection response was also determined by using ultrasound breast muscle thickness (BMT) measurements in combination with bosom breadth (BB) and keel length (KL) values. Methods: The traits analyzed were breast meat weight (BMW), body weight (BW), breast meat percentage (BMP) and the three parameters of breast meat (BB, KL, and BMT). These measurements were derived from studying 15,781 Pekin ducks selected from 10 generations based on breast meat weight. Genetic parameters and breeding value were estimated for the analysis of the breeding process. Results: Estimated heritability of BMW and BMP were moderate (0.23 and 0.16, respectively), and heritability of BW was high (0.48). Other traits such as BB, KL, and BMT indicated moderate heritability ranging between 0.11 and 0.28. Significant phenotypic correlations of BMW with BW and BMP were discovered (p<0.05), and genetic correlations of BMW with BW and BMP were positive and high (0.83 and 0.66, respectively). It was noted that BMW had positive correlations with all the other traits. Generational average estimated breeding values of all traits increased substantially over the course of selection, which demonstrated that the ducks responded efficiently to increased breast meat yield after 10 generations of breeding. Conclusion: The results indicated that duck BMW had the potential to be increased through genetic selection with positive effects on BW and BMP. The ultrasound BMT, in combination with the measurement of BB and KL, is shown to be essential and effective in the process of high breast meat yield duck breeding.

Parameters Estimation of Hinging Hyperplanes using Median Squared Error Criterion

Xiaolin Huang,Jun Xu,Shuning Wang 제어·로봇·시스템학회 2011 International Journal of Control, Automation, and Vol.9 No.4

This paper considers parameter estimation for nonlinear model using median squared error (MSE) criterion, which is limited to linear model in the past. It is shown that applying MSE, the essence of estimating parameters for hinging hyperplanes (HH) and linear model are the same. Motivated by this fact, MSE estimation is discussed for HH. A local optimality condition is given and based on this condition, an algorithm using linear programming technique is proposed. Numerical experiments show the good performance of the proposed estimation strategy and algorithm.

Polymethoxylated Flavone Extracts from Citrus Peels for Use in the Functional Food and Nutraceutical Industry

Xiaolin Yao,Siyi Pan,Chunhong Duan,Fang Yang,Gang Fan,Xinrong Zhu,Shuzhen Yang,Xiaoyun Xu 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.5

Polymethoxylated flavones (PMFs) extracted from Citrus sinensis ‘Jincheng’ peel were characterized by chromatographic and spectroscopic techniques. Seven individual PMF were identified. 3, 3’, 4’, 5, 6, 7-hexamethoxyflavone (HEX), nobiletin (NOB), heptamethoxyflavone (HEP), 5-demethylnobiletin (DN), and tangeretin (TAN) were characterized through electrospray ionization mass spectrometry (ESI-MS) in positive mode of protonated molecular ions [M+H]?, the diagnostic fragment ions, together with the UV-Vis spectra and high performance liquid chromatography (HPLC) elution order from literature data. Sinensetin (SIN) and tetramethyl-O-scutellarein (SCU) were isolated and identified through their MS, ¹H nuclear magnetic resonance (NMR) and UV-Vis spectral studies. The levels of PMFs in peels from different cultivars of citrus fruits grown in China were determined for the first time. The results showed that C. aurantium ‘Bitter orange’ peel was the most promising variety for HEP. C. sinensis peel was a good source for SIN and SCU.

Influence of para-orientating Methoxyl Units on the Electronic Structures and Light Absorption Properties of the Triphenylamine-based dyes by DFT Study

Liang, Guijie,Xu, Jie,Xu, Weilin,Wang, Luoxin,Shen, Xiaolin,Yao, Mu Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.7

The geometries, electronic structures and absorption spectra of the two organic triphenylamine-based dyes TA-St-CA and TA-DM-CA, containing identical electron donors and acceptors but the different conjugated bridges, were studied by density functional theory (DFT) at the B3LYP and PBE1PBE levels, respectively. The influence of para-orientating methoxyl units on the electronic structures and light absorption properties of the dyes and the consequent photovoltaic performance of the dye-sensitized solar cells (DSSCs) were investigated in detail. The results indicate that the introduction of the para-orientating methoxyl units into the conjugated bridge induces the increased absorption wavelength as well as the more negative EHOMO corresponding to the bigger driving force $(E_{I^-/I^-_3}-E_{HOMO})$ for dye reduction, which together improve the photovoltaic performance of TA-DM-CA, although there is a decline of the open circuit voltage caused by the more negative $E_{LUMO}$.

Flexural Behavior of RC Beams with NSM Prestressed Helical Rib Steel Wire

Meixiang Zhang,Xiaolin Yang,Yahong Ding,Ping Xu,Bo Sun,Xiaoyi Zhang,Xianggang Zhang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.9

The reinforcement technique of near-surface mounted?NSM?prestressed helical rib steel wire (PHRSW) with wide raw material sources, lower in price and high application value can significantly enhance the flexural behavior of reinforced-concrete (RC) structures. Based on 8 flexural RC beams reinforced with NSM PHRSW, performance of test beams under different groove widths (10 mm, 15 mm and 20 mm) and concrete strengths (C30 and C40) was analyzed, including load-deflection, ductility, flexural capacity, strain of longitudinal steel bar and HRSW, failure mode and crack propagation. The results show that the overall flexural behavior RC beams reinforced with NSM PHRSW was significantly improved. There is a good entirety bearing capacity between the longitudinal reinforcement and PHRSW of reinforced beam with suitable groove size. The comprehensive performance of reinforced beams increases first then decreases, with the increase of groove size. The higher the strength of concrete, the more significant the influence of groove size. There is an optimal match between groove width and concrete strength for RC beams reinforced with NSM PHRSW. Considering comprehensive property of reinforced beams, groove width with 15mmwas best in this research. The results can make the research of RC beams reinforced with NSM PHRSW more comprehensive and in-depth, and provide design basis for its engineering application.