Transcriptome profiling of rubber tree (Hevea brasiliensis) discovers candidate regulators of the cold stress respo

Xiao‑Xiao Gong,Bing‑Yu Yan,Jin Hu,Cui‑Ping Yang,Yi‑Jian Li,Jin‑Ping Liu,Wen‑Bin Liao 한국유전학회 2018 Genes & Genomics Vol.40 No.11

Tropical plant rubber tree (Hevea brasiliensis) is the sole source of commercial natural rubber and low-temperature stress is the most important limiting factor for its cultivation. To characterize the gene expression profiles of H. brasiliensis under the cold stress and discover the key cold stress-induced genes. Three cDNA libraries, CT (control), LT2 (cold treatment at 4 °C for 2 h) and LT24 (cold treatment at 4 °C for 24 h) were constructed for RNA sequencing (RNA-Seq) and gene expression profiling. Quantitative real time PCR (qRT-PCR) was conducted to validate the RNA-Seq and gene differentially expression results. A total of 1457 and 2328 differentially expressed genes (DEGs) in LT2 and LT24 compared with CT were respectively detected. Most significantly enriched KEGG pathways included flavonoid biosynthesis, phenylpropanoid biosynthesis, plant hormone signal transduction, cutin, suberine and wax biosynthesis, Pentose and glucuronate interconversions, phenylalanine metabolism and starch and sucrose metabolism. A total of 239 transcription factors (TFs) were differentially expressed following 2 h or/and 24 h of cold treatment. Cold-response transcription factor families included ARR-B, B3, BES1, bHLH, C2H, CO-like, Dof, ERF, FAR1, G2-like, GRAS, GRF, HD-ZIP, HSF, LBD, MIKC-MADS, M-type MADS, MYB, MYB-related, NAC, RAV, SRS, TALE, TCP, Trihelix, WOX, WRKY, YABBY and ZF-HD. The genome-wide transcriptional response of rubber tree to the cold treatments were determined and a large number of DEGs were characterized including 239 transcription factors, providing important clues for further elucidation of the mechanisms of cold stress responses in rubber tree.

Prognostic Value of PLCE1 Expression in Upper Gastrointestinal Cancer: a Systematic Review and Meta-analysis

Cui, Xiao-Bin,Peng, Hao,Li, Su,Li, Ting-Ting,Liu, Chun-Xia,Zhang, Shu-Mao,Jin, Ting-Ting,Hu, Jian-Ming,Jiang, Jin-Fang,Liang, Wei-Hua,Li, Na,Li, Li,Chen, Yun-Zhao,Li, Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: A number of studies have identified a shared susceptibility locus in phospholipase C epsilon 1 (PLCE1) for esophageal squamous cell carcinoma (ESCC) and gastric cardia adenocarcinomas (GCA). However, the results of PLCE1 expression in esophageal and gastric cancer remain inconsistent and controversial. Moreover, the effects on clinicopathological features remain undetermined. This study aimed to provide a precise quantification of the association between PLCE1 expression and the risk of ESCC and GCA through meta-analysis. Materials and Methods: Eligible studies were identified from PubMed, Wanfang Data, ISI Web of Science, and the Chinese National Knowledge Infrastructure databases. Using RevMan5.2 software, pooled odds ratios (ORs) with 95% confidence intervals (CIs) were employed to assess the association of PLCE1 expression with clinicopathological features relative to ESCC or GCA. Results: Seven articles were identified, including 761 esophageal and gastric cancer cases and 457 controls. Overall, we determined that PLCE1 expression was associated with tumor progression in both esophageal cancers (pooled OR=5.93; 95%CI=3.86 to 9.11) and gastric cancers (pooled OR=9.73; 95%CI=6.46 to 14.7). Moreover, invasion depth (pooled OR=3.62; 95%CI=2.30 to 5.70) and lymph node metastasis (pooled OR=4.21; 95%CI=2.69 to 6.59) were linked with PLCE1 expression in gastric cancer. However, no significant associations were determined between PLCE1 overexpression and the histologic grade, invasion depth, and lymph node metastasis in esophageal cancer. Conclusions: Our metaanalysis results indicated that upregulated PLCE1 is significantly associated with an increased risk of tumor progression in ESCC and GCA. Therefore, PLCE1 expression can be appropriately regarded as a promising biomarker for ESCC and GCA patients.

《韓國名散文》中國俗語義詞語的漢語翻譯方法硏究

Jin ChangShan,Cui Xiao-Yu 조선대학교 국제문화연구원 2015 국제문화연구 Vol.8 No.1

한 민족어의 의미 체계 속에는 어떤 객체를 표현하는 개념 의미 외에도 해당민족의 역사 문화, 풍속 습관, 민족 정서, 지리 환경등을 표현하는 다양한 문화 상징적 의미도 포함되어 있다. 이런 민족어를 국속(.俗)어휘라고 한다. 한 민족어의 개념의미에 더 첨가된 그 민족만이 갖고 있는 문화 상징적 의미를 국속의미라 하며 이런 국속의미를 연구하는 언어학의 한 분과를 국속의미론이라 한다. 넓은 의미로 말하자면 단어, 성, 속담, 전고 등이 모두 국속어휘에 속한다. 한국어 국속어휘는 한중 문화 교류에서 많이 쓰이고 있으므로 국속어휘의 번역은 단순히 개념의 전환뿐만 아니라 어휘의 은유적인 의미의 다른 문화 간의 전환이라고도 할 수 있어 자못 중요하다. 본 논문의 텍스트 『한국의 명수필』에는 여러 가지 유형의 국속어휘가 씌였는데 그중 단어형 국속어휘가 매우 특징적이다. 단어형 국속어휘는 고유어형 국속어휘와 한자어형 국속어휘 두 가지가 있다. 국속어휘를 온 전하게 번역하면 원문의 뜻을 올바르게 전달할수 있을 뿐만 아니라 독자들의 시야를 넓힐 수도 있다. 본 논문은 『한국의 명수필』에 나타난 단 어형 국속어휘를 대상으로 국속의미론을 적용하여 그 중국어 번역 방법을 모색해 보고자 하였다. 키워드: 국속의미론 국속어휘 중국어 번역

Microwave-Accelerated Click Chemistry: Expeditious Synthesis of Novel Triazole-linked Salicylic β-D-O-Glycosides with PTP1B Inhibitory Activity

Jin-Wei Yang,Cui Li,Xiao-Peng He,Hong Zhao,Li-Xin Gao,Wei Zhang,Xiao-Xin Shi,Yun Tang,Jia Li,Guo-Rong Chen 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.11

The incorporation of microwave irradiation with the prevalent “click chemistry” is currently of considerable synthetic interest. We describe here the introduction of such laboratorial shortcut into carbohydrate-based drug discovery,resulting in the rapid formation of a series of triazole-linked salicylic β-D-O-glycosides with biological activities. All “clicked” products were achieved in excellent yields (≈ 90%) within only a quarter. In addition, based on the structural characteristics of the afforded glycomimetics, their inhibitory activities were evaluated toward protein tyrosine phosphatases 1B (PTP1B) and a panel of homologous protein tyrosine phosphatases (PTPs). Docking simulation was also conducted to plausibly propose binding modes of this glycosyl salicylate series with the enzymatic target.

《韓國名散文》中國俗語義詞語的漢語翻譯方法研究

Jin Chang Shan,Cui Xiao Yu 조선대학교 국제문화연구원 2015 국제문화연구 Vol.8 No.1

한 민족어의 의미 체계 속에는 어떤 객체를 표현하는 개념 의미 외에도 해당민족의 역사 문화, 풍속 습관, 민족 정서, 지리 환경등을 표현하는 다양한 문화 상징적 의미도 포함되어 있다. 이런 민족어를 국속(国俗)어휘라고 한다. 한 민족어의 개념의미에 더 첨가된 그 민족만이 갖고 있는 문화 상징적 의미를 국속의미라 하며 이런 국속의미를 연구하는 언어학의 한 분과를 국속의미론이라 한다. 넓은 의미로 말하자면 단어, 성, 속담, 전고 등이 모두 국속어휘에 속한다. 한국어 국속어휘는 한중 문화 교류에서 많이 쓰이고 있으므로 국속어휘의 번역은 단순히 개념의 전환뿐만 아니라 어휘의 은유적인 의미의 다른 문화 간의 전환이라고도 할 수 있어 자못 중요하다. 본 논문의 텍스트 『한국의 명수필』에는 여러 가지 유형의 국속어휘가 씌였는데 그중 단어형 국속어휘가 매우 특징적이다. 단어형 국속어휘는 고유어형 국속어휘와 한자어형 국속어휘 두 가지가 있다. 국속어휘를 온전하게 번역하면 원문의 뜻을 올바르게 전달할수 있을 뿐만 아니라 독자들의 시야를 넓힐 수도 있다. 본 논문은 『한국의 명수필』에 나타난 단어형 국속어휘를 대상으로 국속의미론을 적용하여 그 중국어 번역 방법을 모색해 보고자 하였다.

Statistical Analysis Techniques of Nonlinear Dynamic System

Xiao Hui Lin,Nai Gang Cui,Yong De Jin 대한전자공학회 1995 ICEIC:International Conference on Electronics, Inf Vol.1 No.1

CCDC26 Gene Polymorphism and Glioblastoma Risk in the Han Chinese Population

Wei, Xiao-Bing,Jin, Tian-Bo,Li, Gang,Geng, Ting-Ting,Zhang, Jia-Yi,Chen, Cui-Ping,Gao, Guo-Dong,Chen, Chao,Gong, Yong-Kuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Background: Glioblastoma (GBM) is an immunosuppressive tumor whose median survival time is only 12-15 months, and patients with GBM have a uniformly poor prognosis. It is known that heredity contributes to formation of glioma, but there are few genetic studies concerning GBM. Materials and Methods: We genotyped six tagging SNPs (tSNP) in Han Chinese GBM and control patients. We used Microsoft Excel and SPSS 16.0 statistical package for statistical analysis and SNP Stats to test for associations between certain tSNPs and risk of GBM in five different models. ORs and 95%CIs were calculated for unconditional logistic-regression analysis with adjustment for age and gender. The SHEsis software platform was applied for analysis of linkage disequilibrium, haplotype construction, and genetic associations at polymorphism loci. Results: We found rs891835 in CCDC26 to be associated with GBM susceptibility at a level of p=0.009. The following genotypes of rs891835 were found to be associated with GBM risk in four different models of gene action: i) genotype GT (OR=2.26; 95%CI, 1.29-3.97; p=0.019) or GG (OR=1.33; 95%CI, 0.23-7.81; p=0.019) in the codominant model; ii) genotypes GT and GG (OR=2.18; 95%CI, 1.26-3.78; p=0.0061) in the dominant model; iii) GT (OR=2.24; 95%CI, 1.28-3.92; p=0.0053) in the overdominant model; iv) the allele G of rs891835 (OR=1.85; 95%CI, 1.14-3.00; p=0.015) in the additive model. In addition, "CG" and "CGGAG" were found by haplotype analysis to be associated with increased GBM risk. In contrast, genotype GG of CCDC26 rs6470745 was associated with decreased GBM risk (OR=0.34; 95%CI, 0.12-1.01; p=0.029) in the recessive model. Conclusions: Our results, combined with those from previous studies, suggest a potential genetic contribution of CCDC26 to GBM progression among Han Chinese.

Microwave-Accelerated Click Chemistry: Expeditious Synthesis of Novel Triazole-linked Salicylic β-D-O-Glycosides with PTP1B Inhibitory Activity

Yang, Jin-Wei,Li, Cui,He, Xiao-Peng,Zhao, Hong,Gao, Li-Xin,Zhang, Wei,Shi, Xiao-Xin,Tang, Yun,Li, Jia,Chen, Guo-Rong Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.11

The incorporation of microwave irradiation with the prevalent "click chemistry" is currently of considerable synthetic interest. We describe here the introduction of such laboratorial shortcut into carbohydrate-based drug discovery, resulting in the rapid formation of a series of triazole-linked salicylic $\beta$-D-O-glycosides with biological activities. All "clicked" products were achieved in excellent yields ($\approx$ 90%) within only a quarter. In addition, based on the structural characteristics of the afforded glycomimetics, their inhibitory activities were evaluated toward protein tyrosine phosphatases 1B (PTP1B) and a panel of homologous protein tyrosine phosphatases (PTPs). Docking simulation was also conducted to plausibly propose binding modes of this glycosyl salicylate series with the enzymatic target.

Expression profiles of microRNAs in skeletal muscle of sheep by deep sequencing

Zhi-Jin Liu,Cun-Yuan Li,Xiao-Yue Li,Yang Yao,Wei Ni,Xiang-Yu Zhang,Yang Cao,Wureli Hazi,Dawei Wang,Renzhe Quan,Shuting Yu,Yuyu Wu,Songmin Niu,Yulong Cui,Yaseen Khan,Shengwei Hu 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.6

Objective: MicroRNAs are a class of endogenous small regulatory RNAs that regulate cell proliferation, differentiation and apoptosis. Recent studies on miRNAs are mainly focused on mice, human and pig. However, the studies on miRNAs in skeletal muscle of sheep are not comprehensive. Methods: RNA-seq technology was used to perform genomic analysis of miRNAs in prenatal and postnatal skeletal muscle of sheep. Targeted genes were predicted using miRanda software and miRNA-mRNA interactions were verified by quantitative real-time polymerase chain reaction. To further investigate the function of miRNAs, candidate targeted genes were enriched for analysis using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment. Results: The results showed total of 1,086 known miRNAs and 40 new candidate miRNAs were detected in prenatal and postnatal skeletal muscle of sheep. In addition, 345 miRNAs (151 up-regulated, 94 down-regulated) were differentially expressed. Moreover, miRanda software was performed to predict targeted genes of miRNAs, resulting in a total of 2,833 predicted targets, especially miR-381 which targeted multiple muscle-related mRNAs. Furthermore, GO and KEGG pathway analysis confirmed that targeted genes of miRNAs were involved in development of skeletal muscles. Conclusion: This study supplements the miRNA database of sheep, which provides valuable information for further study of the biological function of miRNAs in sheep skeletal muscle.

Stimulating Effect of a Novel Synthesized Sulfonamido-Based Gallate ZXHA-TC on Primary Osteoblasts

PAN JIN,Li Zheng,Liang Liao,Xiao Lin,Qinggong Guo,Cui-Wu Lin,Huayu Wu,Jinmin Zhao 연세대학교의과대학 2015 Yonsei medical journal Vol.56 No.3

Purpose: This study is intended to investigate the effects of plants or plant-derived antioxidants on prevention of osteoporosis through the maintenance of reactive oxygenspecies (ROS) at a favorable level. Materials and Methods: In this study, a novel antioxidant, namely 3,4,5-Trihydroxy-N-[4-(5-hydroxy-6-methoxy-pyrimidin-4-ylsulfamoyl)-phenyl]-benzamide (ZXHA-TC) was synthesized from gallic acid and sulfadimoxine. Its effect on osteoblast metabolism was investigated via the detection of cell proliferation, cell viability, production of ROS, and expression of osteogenic-specific genes including runt-related transcription factor 2 (RUNX2), bone sialoprotein (BSP), osteocalcin (OCN), alpha-1 type I collagen (COL1A1), and osteogenic-related proteins after treatment for 2, 4, and 6 days respectively. Results: The results showed that ZXHA-TC has a stimulating effect on the proliferation and osteogenic differentiation of primary osteoblasts by promoting cell proliferation, cell viability, and the expression of genes BSP and OCN. Productions of bone matrix and mineralization were also increased by ZXHA-TC treatment as a result of up-regulationof COL1A1 and alkaline phosphatase (ALP) at the early stage and down-regulationof both genes subsequently. A range of 6.25×10-3 μg/mL to 6.25×10-1 μg/mL is the recommended dose for ZXHA-TC, within which 6.25×10-2 μg/mL showed the best performance. Conclusion: This study may hold promise for the development of a novel agent for the treatment of osteoporosis.