한국재래흑염소에서 발정 및 과배란 유도와 외래유전자 주입에 적합한 1세포기 수정란의 채취

신상태,이두환,김명철,이운규,이철상,한용만,이경광 충남대학교 수의과대학 동물의과학연구소 1998 動物醫科學硏究誌 Vol.6 No.-

Three different treatments for induction of estrus in Korean native black goats were compared: follicle stimulating hormone(FSH, FSH-p^TM), FSH combined with MAP(intravaginal impregnated sponges, Veramix^ⓡ, containing 60 ㎎ medroxy progesterone acetate for 14 days), and FSH combined with progesterone(Ovaron^ⓡ, 10 ㎎ IM for 10 days) and PGF_2α(Lutalyse^ⓡ, 3 ㎎ IM at first FSH injection). FSH for inducing estrus and superovulation was given a total 20 ㎎ intramuscularilly in decreasing dosage injections twice daily over 4 days. The MAPs were withdrawn at the 3rd day of FSH injection. Estrus observations were conducted every 6 hours from last FSH injection for 24 hours by placing the does with fertile male goats. Estrus and superovulation were more successfully induced with treatment of MAP + FSH than other treatments(FSH only, or progesterone + PGF_2α + FSH) (estrus induction; 100 vs 42.8 and 71.4%, ovulation points; 11.4 vs 5.4 and 4.4, respectively). The effect of gonadotropin releasing hormone(GnRH) on the ovulation rate was also examined. However, no difference was observed for inducing ovulation with treatment or dosage(100 ㎍ 200 ㎍) of GnRH. Low midline laparotomies were performed, and then ovarian responses (ovulations and follicular development) were examined by exteriorization of the reproductive tracts. Ova were recovered from oviducts by retrograde flushing 60-146 hours after MAP removal, and were classified the developmental stages. Overall 66.1% (236/357) of recovery rate was obtained from 30 superovulated does. The optimal recovery time of microinjectable 1-cell zygotes was approximately 72-76 hours after MAP removal.

TSC 합성보의 제작방법에 따른 구조적 거동에 관한 실험적 연구

이지운,김도훈,김성배,이창남,김상섭,김규석 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

Composite action can be achieved by providing shear connectors between steel top flange and concrete topping. Composite sections have larger stiffness than the summation of the individual stiffness of slab and beam, therefore, can carry larger load or similar loads with appreciably smaller deflection and are less prone to transient vibration. T-type Steel Composite beam(TSC-beam) is developed and tested. To make the part of steel beam, we used the way of welding two steel plates as well as the way of folding one steel plate.

금속압력용기의 사용 전 음향방출시험

이종오,윤운하,이태희,이종규 한국비파괴검사학회 2003 한국비파괴검사학회지 Vol.23 No.3

사용하지 않은 새 금속 압력용기에 대하여, 사용 전 음향방출시험을 수행할 때 장비점검, 탐촉자 간 거리, 탐촉자 위치, 전 측정 시스템 점검, 가압방법, 잡음 제거법 및 시험 결과의 평가 등에 대한 절차를 수립하고 현장 응용시험이 수행되었다. 일반적으로 음향방출 시험은 많은 잡음을 포함하는데 시험동안 관찰결과를 기록하여 평가 시 시간 필터링함으로서 잡음을 제거할 수 있었다. The field application of acoustic emission(AE) testing for brand-new metal pressure vessel were performed. We will introduce the test procedure for acoustic emission test such as instrument check, distance between sensors, sensor location, whole system calibration, pressurization sequence, noise reduction and evaluation. The data of acoustic emission test contain many noise signal, these noise can be reduced by time filtering which based on the description of observation during AE test.

最近 3年間 本敎室에서 經驗한 腫瘍 253例에 對한 臨床病理組織學的 考察

李揆鶴,高永春,李廷鎭,朴鍾雲,李鍾元,林鍾福,林春悅,朴順道 최신의학사 1964 最新醫學 Vol.7 No.4

이질균의 진단용 단클론 항체 개발에 관한 연구

이연태,조규봉,이운영 단국대학교 신소재기술연구소 1991 신소재 Vol.1 No.-

이질균의 항원구조는 매우 복잡하기 때문에 Shigella 균종 중에서 Shigella flexneri를 선택하여 이질균의 항원 특성을 규명하고자 본 실험을 시도하였다. 그래서 본 교실에서 분리 계대해 온 S. flexneri의 균체 항원을 2회에 걸쳐 마우스의 복강내로 주사하였다. 면역된 마우스의 비장에서 임파구를 분리하여 polyethylene glycol을 이용하여 골수종 세포인 P3X63-Ag8.653과 융합하였다. HAT 배지에서 융합 세포를 선택하였다. 무한대 희석법에 의하여 융합 세포의 클로닝을 실시하였으며 항체의 생산은 간접 효소면역흡착법을 이용하여 확인하고 다음과 같은 결과를 얻었다. 1. 세포 골수종 P3X63-Ag8.653의 평균 분열 주기는 대수 증식기에서 12시간이었다. 2. 융합이 실시된 20개의 plate 중에서 융합 세포가 자란 것은 15 well이었다. 3. 융합 세포가 형성된 15 well에서 항체 형성이 검증된 well은 3개였다. 4. 효소면역흡착법을 통해 알아본 각 well에서 생성된 면역글로불린의 class는 2 well에서 IgG, 1 well에서 IgM이었다. 5. 클로닝 후 IgM의 생성이 확인된 세포 배양 상청액을 이용하여 다른 장내세균의 균체 항원과 반응을 시켰으나 반응이 나타나지 않았다. The antigenic structure of Shigella is very complex. So, production of monoclonal antibodies against Shigella flexneri which had been the most prevalent Shigella spp. in Korea was tried to investigate the characteristics of shigella antigens. Heat-killed cellular antigens of S. flexneri which had been cultivated in our laboratory were injected intraperitoneally into BALB/c mice twice at intervals. Spleen lymphocytes of immunized mice were fused with the myeloma P3X63-Ag8.653 cells using polyethylene glocol. Only hybridized cells grew in HAT medium. Supernatants from cultures were assayed for S. flexneri-specific antibodies by the indirect enzyme-linked immunosorbent assay. Hybridomas were cloned by limiting dilution. 1. The mean doubling time of P3X63-A8.653 myeloma cells was 12 hours. 2. Only 15 wells out of 20 plates cell fusions performed showed the growth of hybridized cells in the selecive medium. 3. S. flexneri-specific antibodies were generated from 3 wells of those 15 wells, checked by indirect ELISA. 4. Supernatants from 3 wells had different classes of immunoglobulins : IgG from 2 wells and IgM from 1 well. 5. After cloning, supernatant from SFB 11 checked as Ig M with the strongest visuality was not reacted with cellular antigens of other strains of Enterobacteriaceae.

일방성 GFRP 복합재료에서 음향방출 신호의 위치표정

이종오,원순호,윤운하,이종규,소철호 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.3

General source location method that use an arrival time differences among sensor is restricted to the composite material and the complex-shaped material. In this study, a bundle-type acoustic sensor composed of 6 pinducers was utilized to determine wave propagation direction and then to estimate source location of the unidirectional GFRP. For the purpose of the study, slowness curve for the material was obtained and made an assumption that the incident waves on pinducers are propagated as a plane wave. According to the results, measured propagation directions of the wave was coincide with theoretical background, however, it was a hassle to determine the source location exactly. But, it is expected that bundle-type sensor gives more accurate results for zone location than generally used acoustic sensors.

마산만 퇴적물의 중금속 및 유기염화합물 오염에 관한 연구

이찬원,민병윤,권영택,양운진,이규환 경남대학교 환경문제연구소 1990 환경연구 Vol.12 No.-

마산내만 및 마산만 유임 주요하천의 오염퇴적물을 1988년 7월부터 1989년 8월에 걸쳐 채취하여 중금속 및 유기염소화합물을 분석하였다. 오염퇴적충의 뚜께는 10㎝에서 169㎝의 넓은 분포를 보였으며 조사지점의 중금속농도는 지점에 따라 많은 차이를 나타내었다. 특히 인근에 마산수출자유지역 및 조선산업이 위치한 조사 지점에서는 모든 중금속의 농도가 가장 높게 나타났다. 마산만 퇴적오염물 중금속 농도를 오염된 일본의 자료와 비교하였을때 Osaka만의 수온을 제외하고 모든 평균농도가 높게 나타났다. 유기염소화합물(PCB 및 DDE)의 농도는 주거지역 및 공장지대가 함께 위치한 산호천 및 마산시 연안지역에서 높게 검출되었다. 본 연구결과에 의하면 마산만과 같은 폐쇄성 해역에서 중금속 오염원으로 공장폐수가 고려될때는 퇴적된 오염물질의 중금속 분석이 좋은 척도가 철 수 있음을 시사하고 있다. Sediments were collected from Masan bay and two main waterways into Masan Bay in the period of July 1988 to August 1989 for the analysis of heavy metals and Organochlorine compounds. The polluted layer of black sludge had a wide range between 10㎝ to 169㎝ and the content of heavy motels was widely distributed from site to site. One site near the Masan free-export area and a shipmaking company was extremely contaminated by heavy metals. All the average concentration of heavy metals in the sediments from Masan Bay were higher than those from Japanese bays compared except Hg from Osaka Bay. Higher levels of PCBs and DDE were found in sediments from Sanho stream and along the coastal area of Masan city. The results obtained in this study imply that the heavy metal analysis of sediments may be a useful to reveal the pollution sources in a closed bay fuck as Masan Bay.

중·고등학생들의 본드남용 실태조사

윤운상,홍관이,노성규,백혜자,주왕기 江原大學校附設體力硏究所 1980 江原大學校附設體育科學硏究所論文集 Vol.- No.5

In the light of recent evidence that the abuse of inhalants is increasing among the Korean youth. A comprehensive inhalants use questionnaire was administered to a sample of 790 Kangweon provincial high school(junior and senior) seniors to determine whether or not a similar trend was evident among this students. Results indicated that 13.2% of the respondents report having tried inhalants in their lifetime, and daily use was reported by 4% among users. Somewhat higher propotions of males(15.9%) than females(10.4%), and TAT※※ high school students(17.2%) than academic high school students(12.6%) are involved in inhalants use. Inhalants users, markedly are more involved in other drugs use and they are dis-satisfied with self and their living environments. 74.0 percent of the students used inhalants for the curiosity.

RTA방법에 의한 Pd- 실리사이드의 쇼트키 장벽 높이에 관한 연구

崔致圭,李雲煥 제주대학교 1988 논문집 Vol.26 No.-

본 연구에서는 RTA방법에 의하여 Pd-Si계에서 우수한 Pd-Si가 형성됨을 확인하였으며 grain의 크기는 약150~200??으로 나타났다. AES스펙럼인 분석에 의하여 Ar 이온으로 sputtering하면서 얻은 Pd와 Si의 조성비는 41.5%와 58.5%로써 Pd- 실리사이드 상이 PdSi상에 거의 비슷함을 알 수 있었으며, 91eV에서 나타난 line shape는 미시구조를 나타내는 PdSi의 특성 피이크임을 보였다. 100~300K의 온도 영역에서 측정한 전류-전압특성곡선으로부터 SBH값은 0.32~0.75eV로 온도가 증가함에 따라 직선적으로 증하하였고 ideality factor n은 일반적인 값 1~2에 만족하였으며 고온영역에서는 거의 1에 근접함을 Tu 등이 실험한 값과 거의 일치됨을 보였다(Herman 1983, Tu, 1981) Si(111)면에서는 100~150K온도범위, Si(100)면에서는 100~200K 온도범위에서는 재결합 생성중심에 의한 전류흐름을 보여 열처리시 defect를 제거하는데 충분하지 못한다는 것을 알 수가 있었다. 다라서 RTA방법에 의한 실리사이드 형성시 실리콘 표면 영역에서 발생한는 defect를 제거하는 방법만 보완시키면 PdSi접촉을 이용하는 어떤 바이폴라 집적회로의 응용에도 가능하며 RTA는 장시간 열처리를 수반하는 전기로 열처리 방법보다 더 간편하고, 소자의 다른 특성을 변화시키지 않으므로 전이금속 실리사이드 형성에 우수한 장치로 평가된다. Formation of the palladium silicides was performed by the rapid thermal annealing using quartaz-halogen tungsten lamps. The Schottky-barrier heights of palladium silicides on both n-type(100)and(111)-oriented Si wafers have been measrued in the temperature range 100-300k with use of a current-voltoge thechnique. Silicide formation was monitored by glancing -angle X-ray diffraction and AES. The values of the barrier heights are 0.32-0.74eV for Si(111) and 0.34-0.75eV for Si(100) at temperaure in the range 100-300k. The barrier heights depend primarily on the metal deposited and not on the partivular silicide phase.

교량의 실시간 거동 모니터링을 위한 GPS의 적용

박운용,정성모,곽두호,이흥규 東亞大學校 大學院 1999 大學院論文集 Vol.24 No.-

Real Time Kinematic GPS will allow a multitude of monitoring, navigation and engineering applications. Applications inclued real time deformation and movement monitoring and real time automated plant control. In a low dynamic environment, with a short baseline length, it is possible to position a roving receiver to an approximate accuracy of less than 5mm within a few seconds of thestart of the ambiguity. One Engineering application is the monitoring of the movement of cable suspension bridges. Such structures are capable of movements of up to a few meters under adverse wind conditions or heavy traffic loads. The use of relative OTF positioing at strategic points upon the bridge would allow close monitoring of the bridge's movements and calculate deformation and long-term deterioration factors for the bridge. Future bridge designs and traffic management schemes could also benefit from the monitoring system.