生物學的 窒素·隣除去에 關한 硏究

金元滿,柳亨烈 漢陽大學校 環境科學硏究所 1991 環境科學論文集 Vol.12 No.-

富營養化(Eutrophication) 影響物質인 窒素와 燐의 除去方法中 N·P 除去를 위한 生物學的高度處理方法中의 하나인 A₂/O 工法(Anaerobic Anoxic/Oxic Process)에서 除去效率增大를 위해 사용하는 메탄올의 경제적 대처방안의 하나로 一次沈澱池의 沈澱物을 嫌氣性消化시켜 그 탈리액을 炭素源으로 사용하였다. 炭素源의 공급없이 운전되는 A₂/O 工法과 嫌氣性消化槽를 설치한 A₂/O 工法의 두가지 Process에서 內部反送率을 변화시킴으로서 얻어지는 處理效果를 비교검토 하였다. 硏究結果, 두가지 Process에서 反送率이 燐 除去에 미치는 영향은 미미한 것으로 나타났으며, 窒素除去에 있어서는 反送率이 클수록 양호하게 나타났다. 嫌氣性消化槽 設置有·無에 따른 處理效率 比較에서는 流入原水 BOD의 감소에 따라 燐濃度가 적정비율을 초과하게 되는 경향이 있어서 설치하지 않은쪽의 處理效率이 더 우수한 결과를 나타냈다. The Anaerobic-Anoxic/Oxic process is one of the biological treatment methods to remove nitrogen and phosphate effectively which are nutritional elements for entrophication. Supernatant of primary sediment of Anaerobic digester is used as a carbon source instead of methanol supply in the usual A₂/O process. The efficiency of the following treatment processes are as follows; 1. Changing recycle rate in the usual A₂/O process without the stage of anaerobic disgester. 2. Changing recycle rate in the usual A₂/O process with the supernant supply of the anaerobic digester. In the result of comparison, changing recycle rate is almost no effect in the removal of phosphate, however the effect of removal in nitrogenous substance are remarkable, and the effect of anaerobic digester is not as effective as expected because the BOD removed in the digester partly, the rate of phosphates to the BOD exceed pertinent range.

LT, Others : PE-133 ; A case of hepatic hemangioma appearing atypical finding confirmed by dynamic MRI in patients with high risk of hepatocellular carcinoma

( Dong Won Park1 ),( Soo Hyung Ryu1 ),( Myung Ki Oh ),( Seong Yeon Jeong ),( Kyeong Sam Ok ),( Jae Chan Shim ),( Jin Nam Kim ),( You Sun Kim ),( Jeong Seop Moon ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background: Hemangioma is the most common benign hepatic tumor. Many hepatic hemangiomas tend to be found incidentally, but should be differentiated from malignant tumors. We report an interesting case of hepatic hemangioma in patient who has high risks of hepatocellular carcinoma. Case: A 52-year-old woman, who was a chronic alcoholic abuser and hepatitis C virus carrier with no treatment experience, was admitted due to diarrhea. The patient had suffered from intermittent abdominal pain in the right subcostal area for recent 3 months. Also the patient had easy bruising and frequent epistaxis. She had used oral contraceptives for almost 20 years. Systolic blood pressure was 160 mmHg and diastolic blood pressure was 80 mmHg. Body temperature was normal. Physical examination revealed icteric sclerae and tenderness in right upper quadrant of the abdomen. Laboratory study showed platelet count of 31,000/ul, AST/ALT of 302/127 IU/L, and total bilirubin of 2.7 mg/dl. 3-phased contrast-enhanced abdominal computed tomography (CT) revealed a 4 cm × 4 cm sized hepatic mass involving both segment 5 and 6. Abdominal CT finding suggested hepatic hemangioma, however, typical finding of hemagioma was unclear, therefore, could not rule out the malignancy. Since she had risk factors for hepatocellular carcinoma, abdominal ultrasonography was performed for further evaluation. But abdominal ultrasonography also showed atypical finding. The confirmative diagnosis could be done by dynamic magnetic resonance imaging (MRI) using primovist®, which is an innovative liver cell-specific contrast medium. Dynamic MRI presented peripheral globular enhancement and a centripetal fill-in pattern, confirmatory findings of hepatic hemangioma, on the delayed image. The patient got relief from abdominal pain and diarrhea during admission, now is being followed-up at a general out-patient clinics. Conclusions: Hemangiomas with atypical abdominal CT and ultrasonographic findings in patients with high risks of hepatocellular carcinoma can be easily characterized with primovist-enhanced dynamic MRI.

알루미늄 연속주조 용탕의 탈 가스 일체화 장치 개발

이용중,김태원,김기대,류재엽,이형우 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

It is necessary for managing a perfect process for degasing aluminum molten metal according to the increase of a grade of aluminum and its alloy products. There are some methods that have been used to manage a degasing process in recent years, such as an injection method that uses aluminum molten metal powder and chemicals supplier and input method that supplies argon and nitrogen, or chlorine gas by using a gas blow-tube. However, these methods show some problems, and it shows that it is a difficult process to handle, pollution due to the producing a lot of toxic gases like chlorine and fluoride gas, irregular effects, and lowering work efficiency due to the excessive processing time. The problems that are the most fatal are the producing a lot of sludge due to the reaction of aluminum molten metal with chemicals, loss of metals, and decreasing the life of refractory materials. In order to solve these problems, this paper develops a technology that is related to aluminum continuous casting molten metal and monolithic degasing apparatus. A degasing apparatus developed in this study improved the existing methods and prevented environmental pollution with smokeless, odorless, and harmlessness by using a new method that applies argon and nitrogen gas in which the methods used in the West and Japan are eliminated. The developed method can significantly reduce product faults that are caused by the production of gas and oxidation because it uses a preprocessed molten metal with chemicals. In addition, the amount of the produced sludge can also be reduced by 60~80% maximum compared with the existing methods. Then, it makes it possible to minimize the loss of metals. Moreover, the molten metal processing and settling time is also shortened by comparing it with the existing methods that are applied by using chemicals. In addition, it does much to improve the workers' health, safety and environment because there is no pollution. The improvement of productivity and prevention effects of disaster from the results of the development can be summarized as follows. It will contribute to the process rationalization because it does not have any unnecessary processes that the molten metal will be moved to an agitator by using a ladle and returned to process for degasing like the existing process due to the monolithic configuration. There are no floating impurities due to the oxidation caused by the contact with the air as same as the existing process. In addition, it can protect the blending of precipitation impurities. Because it has a monolithic configuration, it can avoid the use of additional energy to compensate the temperature decreasing about 60? that is caused by the moving of molten metal. It is not necessary to invest an extra facilities in order to discharge the gas generated from a degasing process by using an agitator. The working environment can be improved by the hospitable air in the factory because the molten metal is almost not exposed in the interior of the area.

농장 사육되는 타조에서 나타난 다리 기형의 증례 보고

최종윤,손화영,전무형,조성환,김혜성,류시윤,박배근,이영원,윤원기 충남대학교 수의과대학 동물의과학연구소 2001 動物醫科學硏究誌 Vol.9 No.-

Two farmed young ostrich chicks were applied to pathology laboratory at Chungnam National University. Grossly, one ostrich showed rotated tibiotarsus, the other showed rolled toes. Case 1 ostrich was hypertrophy of fibualis longus and tibial cranialis tendon and gastrocnemius tendon in crus, inflammation of flexor digitorum longus. In histopathological views, myocytes of fiburalis longus were showed hypertrophy and hyperplasia. It is not found any specific feature in X-ray of the case 2 ostrich. Limb deformities were detected frequently in farmed ostrich chicks in 2- to 3 weeks old. Limb deformities were an important cause of mortality in farmed ostrich chicks during the first 10weeks after hatch. Cause of limb deformities were considered a nutrition problem.

우라늄 이온의 선택흡착

정종헌,김준수,오원진,유재형,유승곤 한국공업화학회 2002 응용화학 Vol.6 No.1

Uranium-containing aqueous wastes have been treated by electrosorption on a carbon electrode composed of activated carbon fibers(ACFs) in a continuous flow-through cell. Effective uranium (VI) removal is accomplished when a negative potential in the range of -0.3 to -0.9V (vs. Ag/AgCI) is applied to the carbon electrode. For a feed concentration of 350mg/l, the concentration of U(VI) in the cell effluent is reduced to less than lmg/l. Electrosorption capacity over 552 mg_uranium/g_ACF is reached.

돼지생식기호흡기 증후군(PRRS) 바이러스 감염시킨 돼지 기관의 병리학적 관찰

조성환,김현수,윤원기,전무형,류시윤,박배근,손화영,김무강 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The porcine reproductive and respiratory syndrome virus was inoculated into swine tracheal organ culture. The ballooning degeneration of the tracheal ciliated epithelium and a decrease of ciliary activity from the tracheal organs infected with PRRS virus were observed one day post-infection(PI). Tracheal epithelium showed degeneration and focal necrosis 2 days PI. Epithelial necrosis and loss of cilia and epithelial cells became more severe 3 and 4 days PI. An entire loss of cilia and epithelial cells were recognized 4 days PI, however, control tracheal organs were normal during the period of the experiment except a little decrease of ciliary activities with time..

Comparison of secondary metabolite changes in Camellia sinensis leaves depending on the growth stage

Ryu, Hyung Won,Yuk, Heung Joo,An, Ju Hyeon,Kim, Doo-Young,Song, Hyuk-Hwan,Oh, Sei-Ryang Elsevier Science B.V., Amsterdam. 2017 FOOD CONTROL Vol.73 No.-

Inhibition of Xanthine Oxidase by Phenolic Phytochemicals from Broussonetia papyrifera

Ryu, Hyung Won,Lee, Ji Hye,Kang, Jae Eun,Jin, Young Min,Park, Ki Hun 한국응용생명화학회 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.5

The roots of Broussonetia papyrifera were extracted into four different polar solvents: chloroform, 50% ethanol in water, ethanol, and water. The ethanol extract showed the most potent inhibition (72.3% at 20 g/mL) against xanthine oxidase (XOD). Chromatography of EE yielded nine phenolic phytochemicals, which were confirmed as broussochalcone A (1), broussochalcone B (2), 3,4-dihydroxyisolonchocarpin (3), 4-hydroxyisolonchocarpin (4), 3-'(3-methylbut-2-enyl)-3',4',7-trihydroxyflavane (5), kazinol A (6), kazinol B (7), kazinol E (8), and broussoflavan A (9). All isolated compounds (19) possessed potent antioxidant activities against 2,2-diphenyl-l-picrylhydrazyl and 2,2'-azino-bis-ethylbenzthiazoline-6-sulfonic acid (ABTS) radicals with $IC_{50}$ values ranging from 5.8 to 252.8M. Although most compounds exhibited potent inhibition with $IC_{50}$ values ranging 0.6-164 M against XOD, compounds 1 and 3 were found to be the principal contributors to the XOD inhibition in ethanol extract. The analysis of $K_I$ and $K_{IS}$ values proved that the two most promising compounds (1 and 3), present at high concentrations in the root barks as analyzed by using high-performance liquid chromatography analysis, were reversible mixed type I inhibitors.

Anti-inflammatory flavonoids from root bark of <i>Broussonetia papyrifera</i> in LPS-stimulated RAW264.7 cells

Ryu, Hyung Won,Park, Mi Hyeon,Kwon, Ok-Kyoung,Kim, Doo-Young,Hwang, Jung-Yeon,Jo, Yang Hee,Ahn, Kyung-Seop,Hwang, Bang Yeon,Oh, Sei-Ryang Elsevier 2019 Bioorganic chemistry Vol.92 No.-

<P><B>Abstract</B></P> <P> <I>Broussonetia papyrifera</I> has been used as a diuretic, tonic and suppressor of edema. Bioactivity-guided fractionation and metabolite investigation of root bark extracts of this plant resulted in the isolation and identification of six 1,3-diphenylpropanes (<B>1</B>, <B>2</B>, <B>8</B>, <B>10</B>, <B>17</B>, <B>20</B>), flavanone (<B>3</B>), two chalcones (<B>4, 5</B>), five flavans (<B>6</B>, <B>11</B>, <B>14</B>–<B>16</B>), dihydroflavonol (<B>7</B>) and five flavonols (<B>9</B>, <B>12</B>, <B>13</B>, <B>18</B>, <B>19</B>), including five new compounds (<B>5</B>, <B>7</B>, <B>8</B>, <B>19</B>, <B>20</B>) that inhibit NO production in LPS-induced RAW264.7 cells. The structures of compounds <B>1</B>–<B>20</B> were elucidated on the basis of spectroscopic data (1D and 2D NMR, MS, MS/MS, and HRMS). In particular, compounds <B>3</B>, <B>5</B>, <B>7</B>, <B>12</B>, and <B>20</B> exhibited significant inhibitory effects on the NO, iNOS, and pro-inflammatory cytokine (TNF-α and IL-6) production. Therefore, this study suggests that the flavonoid-rich products of <I>B. papyrifera,</I> including the new compounds, could be valuable candidates for the development of pharmaceuticals or functional foods in the prevention and treatment of anti-inflammatory disease.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The potent anti-inflammation was observed on <I>Broussonetia papyrifera</I> root bark. </LI> <LI> Five new and fifteen known compounds were isolated from the active fractions of <I>B. papyrifera</I>. </LI> <LI> The compounds were evaluated for inhibition of NO, iNOS, and pro-inflammatory cytokines. </LI> <LI> The new compounds <B>5</B>, <B>7</B>, <B>8</B>, and <B>20</B> showed anti-inflammatory effects dose-dependently. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Anthocyanin Contents Enhancement with Gamma Irradiated Mutagenesis in Blackberry (Rubus fructicosus)

Ryu, Hyung Won,Cho, Byoung Ok,Ryu, Jaihyunk,Jin, Chang Hyun,Kim, Jin-Baek,Kang, Si Yong,Han, Ah-Reum NATURAL PRODUCT COMMUNICATIONS 2017 Natural product communications Vol.12 No.9

<P>Effects of radiation processing on the endogenous phytochemical production in blackberry (Rubus fructicosus L.) were investigated through a metabolites analysis, using high-performance liquid chromatography with a diode array detector and a triple quadrupole mass spectrometer (HPLC-DAD-ESI-MS/MS). Four mutant cultivar lines (B, C, D, and E) were developed through the treatment of various doses of gamma irradiation (80, 60, 40, and 20 Gy) on the control blackberry cultivar (V3). These mutant cultivar lines were selected according to their different morphological variations in leaf shape and branch color. In the present study, we performed quantitative analyses of the four standard compounds in blackberry mutant cultivars: cyanidin-3-O-glucoside, cyanidin-3-Oxyloside, cyanidin-3-O-malonylglucoside, and cyanidin-3-O-dioxalylglucoside. The HPLC fingerprints displayed different profiles for six blackberry cultivar lines, demonstrating that the accumulation of total anthocyanins was affected by the gamma irradiation. The contents of cyanidin-3-O-glucoside and cyanidin-3-O-xyloside were high in the gamma-irradiated mutant cultivars of blackberries, compared to those of the control cultivar (V3) and the cross breeding cultivar (R. fructicosus x R. parvifolius; CB). Cyanidin-3-O-malonylglucoside and cyanidin-3-O-dioxalylglucoside were also analyzed for their quantification in six cultivar lines, as metabolites contributing to the discrimination of six cultivar lines. Among gamma irradiated mutant blackberry cultivars, the highest dose level of gamma irradiation (80 Gy) resulted in the highest cyanidin-3-O-glucoside content enhancement. Therefore, these results can be useful to determine the optimized gamma irradiated value at which anthocyanins reaches an abundant level in Rubus species.</P>