p-Anisaldehyde가 조골세포 분화에 미치는 영향

김경민 ․ 이도원 ․ 김현준 ․ 김아랑 ․ 장원구 대구대학교 산업기술연구소 2018 産業技術硏究 Vol.29 No.2

p-Anisaldehyde is a natural fragrance extracted from Pimpinella anisum L., and used as a preservative. This study examined the effect of p-anisaldehyde on osteoblast differentiation. First, cytotoxicity tests were carried out after examining the concentrations that did not show toxicity to the cells. The expression of osteoblast differentiation marker gene was confirmed by RT-PCR and was not effective in the treatment with p-anisaldehyde alone. However, p-anisaldehyde decreased the expression levels of inhibitor of differentiation-1 (Id1), distalless related homeobox (Dlx5), and runt-related transcription factor 2 (Runx2), which are osteogenic differentiation marker genes, which are increased in osteogenic condition. alkaline phosphatase (ALP) activity was also confirmed by ALP staining that p-anisaldehyde reduced ALP activity. These results show that p-anisaldehyde is effective in reducing osteoblast differentiation.

전경대원에서 발생한 결핵의 임상 양상

문창기,박상준,조민구,김영중,김소연,김윤권,정준오,안석진,김은실,서승오,김지훈,최원제,이윤영,박형기,최규영,김현근 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background: The purpose of this study was to document the incidence and clinical characteristics of patients with tuberculosis (TB) in combat and auxiliary police, living in a group, in Korea where the incidence rate of active TB in a general population is higher than in Western countries. Materials and Methods:We retrospectively reviewed the medical records of all tuberculosis patients diagnosed at National Police Hospital from January 2002 through December 2004. Results:In 2002-2004, a total of 156 cases of tuberculosis were identified with the mean (Standard deviation) age of 20.6 (±1.0) years. Of these, 134 (85.9%) patients were registered as new cases, 11 (7.1%) as relapse, 2 (1.3%) as failure, 5 (3.1%) as treatment-after-default cases whereas 4 (2.6 %) patients were not included in any categories. Average annual new TB rate and smear-positive TB rate were 86.5/10^(5) and 17.4/10^(5) person-years, respectively. In 12 of 31 smear-positive cases, time from onset of symptoms to diagnosis was more than 30 days. Two multidrug-resistance TB cases were identified and two suspected outbreak episodes of TB had occurred during 3 years. Conclusion:There was no statistically significant difference in the incidence rate of new cases of TB between the general population aged 20 to 29 years and combat and auxiliary police in Korea. 배경 : 국내 결핵의 발생은 감소 추세에 있으나 여전히 후진국형 발생양상을 보이고 있다. 특히 집단생활을 하는 젊은이들에서 발생하는 결핵의 유행이 간헐적으로 알려지고 있어 우려를 낳고 있다. 이에 저자들은 최근 전경대원의 결핵 발병 양상에 대해 알아보고자 본 연구를 시행하였다. 재료 및 방법 : 2002년 1월부터 2004년 12월까지 3년간 경찰병원에서 결핵 진료를 받은 전경대원의 의무기록을 후향적으로 분석하여 연도별 결핵 신환발생률, 도말양성결핵 신환율, 진단 지연, 집단발병, 다제내성결핵을 조사하였다. 결과 : 연도별 결핵 신환자 수(10만명당 발생추정치)는 2002년 50명(83.6명), 2003년 42명(83.7명), 2004년 42명(93.5명)이었고 도말양성 폐결핵 신환자 수는 2002년 10명(16.7명), 2003년 11명(21.9명), 2004년 6명(13.4명)이었다. 3년간 도말양성 폐결핵 환자는 31명이 있었고 그 중 12명은 호흡기 증상 발생 30일이 지나서 진단을 받았다. 다제내성 결핵은 2명이 있었으며 결핵의 집단발병이 의심되는 사례는 2차례 있었다. 결론 : 전경대원과 20-29세 연령군 일반인의 결핵 신환발생률, 도말양성 폐결핵 발생률의 통계적으로 유의한 차이는 없었다.

Medicinal Chemistry : EVALUATION OF CADMIUM-INDUCED NEPHROTOXICITY USING URINARY METABOLOMIC PROFILES IN SPRAGUE-DAWLEY MALE RATS

( Yu Kyung Lee ),( Eun Young Park ),( Shi Won Kim ),( Ji Yeon Son ),( Tae Hyung Kim ),( Won Gu Kang ),( Tae Chun Jeong ),( Kyu Bong Kim ),( Seung Jun Kwack ),( Jae Won Lee ),( Suh Mann Kim ),( Byung M 영남대학교 약품개발연구소 2015 영남대학교 약품개발연구소 연구업적집 Vol.25 No.-

The aim of this study was to investigate urinary metabolomics profiles associated with cadmium (Cd)-induced nephrotoxicity and their potential mechanisms. Metabolomic profiles were measured by high-resolution 1H-nuclear magnetic resonance (NMR) spectroscopy in the urine of rats after oral exposure to CdCl2 (1, 5, or 25 mg/kg) for 6 wk. The spectral data were further analyzed by a multivariate analysis to identify specific urinary metabolites. Urinary excretion levels of protein biomarkers were also measured and CdCl2 accumulated dose-dependently in the kidney. High-dose (25 mg/kg) CdCl2 exposure significantly increased serum blood urea nitrogen (BUN), but serum creatinine (sCr) levels were unchanged. High-dose CdCl2 (25 mg/kg) exposure also significantly elevated protein-based urinary biomarkers including osteopontin, monocyte chemoattractant protein-1 (MCP-1), kidney injury molecules-1 (Kim-1), and selenium-bingding protein 1 (SBP1) in rat urine. Under these conditions, six urinary metabolites (citarate, serine, 3-hydroxyisovalerate, 4-hydroxyphentllactate, dimethylamine, and betaine) were involved in mitochondrial energy metabolism. In addition, a few number of amino acids such as glycine, glutamate, tyrosine, proline, or phentlalanine and carbohydrate (glucose) were altered in urine afrer CdCl2 exposure. In particular, the metabolites involved in the glutathione biosynthesis pathway, including cysteine, serine, methionine, and glutamate, were markedly decreased compared to the control. Thus, these metabolites are potential biomarkers for detection of Cd-induced nephrotoxicity. Our results further indicate that redox metabolomics pathways may be associated with Cd-mediated chronic kidney injury. These findings provide a biochemical pathway for better understanding of cellulat mechanism underlying Cd-induced renal injury in hunmans.

GMM을 적용한 음성변환방법

구원모,류석한,박현숙,김창석 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

This paper was a study on method that transforms a source speaker voice in order to be heard like voice of a specified speaker. It was able to setimate speech signal feature with proposed MFCC method considering property of human auditory. Applying EM algorithm for estimated feature valuse optimal parameter of GMM, we can compose GMM distribution. Composed GMM determined a weighting value of frequency envelope on speech conversion function. With result of experiment applying HNM in order to convert speech, we can verify that speech signal frequency envelope of a source speaker was similarly converted to speech signal frequency envelope fo a specified speaker.

滌痰湯이 흰쥐의 高脂血症에 미치는 영향에 관한 實驗的 연구

김근우,구병수,이원철 동국대학교 경주대학 1997 東國論集 Vol.16 No.2

This study was done in order to investigate the effect of choˇkda~m-t'ang on hyperlipidemia. Methods: Hyperlipidemia was induced in male rats by oral administration of cholesterol(50㎎/㎏) diet for 45 days. To carry out an experiment, the rats was divided into three groups. The first group(normal group) was fed on water for 45 days. The second group(control group) was administered 5% ethanol instead of water only for first 15 days and subsequently administered cholesterol(50㎎/㎏ p.o) diet with 5% ethanol for the second 15 days and fed on a solution of salt for the last 15 days during total 45 days. The third group(sample group) was administered in the same way with the control group for 30 days and injected choˇkda~m-t'ang extract(100㎎/㎏) for the last 15 days during total 45 days. And measured the levels of serum total cholesterol, serum HDL-cholesterol, serum total lipid, serum triglyceride, serum phospholipid, hepatic lipid peroxide and activity of serum AST·ALT. Results: 1. From fifteenth day, the level of total cholesterol was decreased with the significance in the sample group as compared with the control group. 2. The level of serum HDL-cholesterol was increased in the sample group compared with control group, but did not show significance. 3. The level of serum total lipid was decreased with significance in the sample group as compared with control group. 4. The level of serum triglyceride was decreased with significance in the sample group as compared with control group. 5. The level of serum phospholipid was decreased in the sample group compared with control group, but did not show significance. 6. The level of hepatic lipid peroxide was decreased with significance in the sample group as compared with control group. 7. The activity of serum ALT was decreased with significance in sample group as compared with control group, but that of serum AST was not significance. Conclusion According to above results, it is considered that choˇkda~m-t'ang injection groups have recovering effect on the hyperlipidemia induced by oral cholesterol administration in male rats. This might be suggest that choˇkda~m-t'ang have recovering effect on arteriosclerosis and cerebral vascular accidents. Choˇkda~m-t'ang increase body's capacity of controling abnormal lipid metabolism, it can be used to prevent hyperlipidemia and related diseases.

Sol-Gel방법에 의해서 성장시킨PbTiO₃박막의 결정화와 물리적 성질

구영진,강민진,정원태,김태현,김상수 昌原大學校 基礎科學硏究所 2000 基礎科學硏究所論文集 Vol.12 No.-

Ferroelectric PbTiO3 thin films coated on SiO2/Si(100) and ITO glass substrates were prepared by sol-gel processing of metal alkoxide solutions and solvents. The use of sol-gel method for preparing various functional films makes it possible for the homogeneous distribution of elements at the molecular level, precise composition control, lower temperature synthesis, large surface coating and high optical quality. First of all, the XRD investigation shows that perovskite phase of polycrystalline PbTiO3 was formed by four times coating of the concentrated solution and by the heat treatment over 500, 600℃(O2 gas and air) and over 10, 20, 30 min. Surfaces of thin films were crack-free and uniform and the average grain size investigated by SEM was 0.25∼1.00 ㎛. Dielectric constant of PbTiO3/ITO glass thin film were 380 ∼ 5.25 in the region of 1 kHz∼ 10 MHz at room temperature(30℃), respectively. From the hysteresis loop of PbTiO3/ITO glass thin film, a remnant polarization of 0.382 μC/cm2 and a coercive filed of 23.85 kV/cm were obtained. At the frequency(5 kHz), C-V measurements of films in MFIS and MFM configurations showed well-behaved C-V characteristics and an injection type hysteresis. The capacitance of flat band(CFB) was 60 pF. The band gap energies of the thin films depend on the assumption of direct or indirect transition. Using one layer structure model, we calculated refractive index, extinction coefficient, absorption coefficient and band gap energy of thin film in the wavelength range of 300 to 800 nm. The band gap energy, refractive index and extinction coefficient at λ = 6325 Å of the PbTiO3 thin film were 3.7 eV, 1.96 and 0.1 respectively.

유치 치주인대 신경분포에 관한 면역조직화학적 연구

구대학,이원재,배용철,김영진 大韓小兒齒科學會 1994 大韓小兒齒科學會誌 Vol.21 No.2

생리적 치근 흡수가 뒤따르는 유치 치주인대의 신경분포 및 변화를 평가하기 위하여 생후 1, 2, 3, 4, 5개월된 개의 유치 치주인대와 9개월된 영구치 치주인대를 대상으로 neurofilament protein(NFP)을 이용한 면역조직화학적 방법으로 관찰하여 다음과 같은 결과를 얻었다. ● 유견 유치의 치주인대 신경분포는 치근단 1/3부위에서 가장 밀집되어 있었으며 치근 중앙부로 향할수록 현저하게 감소되었으며 전체적으로 그 분포밀도 및 분지 정도는 영구치와 비교시 약한 양상을 나타냈다. ● 구치부에 비해 전치부에서 신경분포가 보다 발달된 양상을 보였으며 구치 치근이개부치주인대에서는 신경섬유가 관찰되지 않았다. ● 유치근이 흡수됨에 따라 신경분포 정도도 감소되었으며 탈락시기의 치근 주위에서는 신경섬유룰 관찰할 수 없었다. ● 치은점막상피의 신경지배는 치아가 발육함에 따라 감소되는 양상을 나타내었으며 설측점막이 순측점막보다 더 발달된 신경분포양상을 관찰할 수 있었다. ● 유치 치주인대 신경말단 형태는 수지상의 신경말단 형태를 나타내고 있었으며 전형적인 Ruffini 신경말단은 거의 관찰할 수 없었다. 이상의 결과를 미루어 신경섬유의 분포는 기능과 밀접한 관계가 있는 것으로 생각되고 더욱이 유치의 생리적 치근흡수에 따른 치주인대에 신경섬유의 분포 및 변화는 유치의 성장발육에 따른 하나의 변화라고 생각된다. The purpose of this study was to investigate the distribution of nerves in the periodontal ligament of a dog's primary teeth by each developing stage. The distribution of nerves in the periodontal ligament were investigated by means of immunohistochemistry for detection of neurofilament protein(NFP). The results were as follows: The NFP-immunoreactive nerve fibers were found to be densely distributed in the apical third of the periodontal ligament, while they were sparse in the coronal two third, in both primary and permanent teeth. In generally the density of distribution and degree of arborization of nerve fibers in periodontal ligament of primary teeth revealed a poor appearance compared with that of permanent teeth. Periodontal ligament in anterior teeth showed more abundant nerve innervation than posterior teeth, and the periodontal ligament of the bifurcation area in posterior teeth roots were not observed to have nerve fiber. The density of nerve distribution in the periodontal ligament of primary teeth was reduced according to the physiological root resorption and nerve fibers were not observed in the surrounding area on the root of the exfoliation stage in primary teeth. The distribution of nerve fibers in mucogingival tissue, was poor innervated according to the aging of the dogs. A more abundant distribution of nerve fiber was represented in the lingual mucogingival tissue than in the labial side. Most of the nerve endings in the periodontal ligament of primary teeth showed a tree-like appearance. However, the typical Ruffini-like nerve endings were not observed.

EZW를 적용한 영상압축에 관한 연구

具元謨,金昌錫 明知大學校 産業技術硏究所 2002 産業技術硏究所論文集 Vol.21 No.-

The EZW algorithm is encoded by zerotree coding technique using serif-similarity of wavelet coefficients. If the coefficient is larger than the threshold a POS coded, if the coefficients is smaller than minus the threshold a NEG is coded. If the coefficient is the root of a zerotree than a ZTR is coded and finally, if yhe coefficient is smaller then the threshold but it is not the root of a zerotree, than an IZ is coded. This process is repealed until all the wavelet coefficients have been encoded compleatly. This paper was compared to EZW algorithm and a widely available version of JPEG. As the results of compare, it is shown that the PSNR of the EZW algorithm is bettor than JPEG.

혈관과증식을 동반한 구개부 선방세포암의 치험례

김상중,심재환,박정훈,이원학,이정구 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.6

Vascular lesions of the oral and maxillofacial area is interesting to clinicians because it is difficult in management and has poor prognosis. There have been attempt to classify vascular lesion and recently new classification is introduced that have two basic entities, 1) hemangioma, 2) vascular malformation. It is useful that evaluation of lesion, treatment planning and predicting on prognosis. But vascular lesion is still difficult to clinicians about treatment. A case of acidic cell carcinoma combined with hypervascularity arising on the hard palate in a 28-year-old woman is reported. We mistook it for vascular lesion because of hypervascularity. The clinical and histologic feature are described. Literatures about neovascularization of tumor & acidic cell carcinoma are reviewd, too. And we attempt to solve the problem of hypervascularity.

갑상선기능저하증에 합병된 중증 전자간증과 심외막 삼출액으로 의한 심장탐폰을 동반한 임신 1예

구희주,최원석,이지영,손인숙,김수녕 건국대학교 의과학연구소 2001 건국의과학학술지 Vol.11 No.-

Hypothyroidism presenting during pregnancy is rare, probably due to the lower incidence of hypothyroidism during reproductive years. And hypothyroidism rarely complicates pregnancy because most hypothyroidism is accompanied by amenorrhea and anovulation. Risk of maternal complications of hypothyroidism including preeclampsia, placental abruption, anemia, postpartum hemorrhage, cardiac dysfunction are increased. Hypothyroidism accompanies pericardial effusion frequently. But the diagnosis of pericardial effusion is usually made incidentally by routine check-up because the patient rarely complains any symptoms of pericardial effusion. Moreover it is extremely rare that pericardial effusion makes the presenting sign of hypothyroidism. We experienced a case of preeclampsia with pericardial effusion in hypothyroid parturient that needed pericardial window operation with emergency cesarean section. So we present this case with a brief review.