Single Nucleotide Polymorphisms of the GnRHR Gene Associated with Reproductive Traits of Japanese Flounder (Paralichthys olivaceus)

He, Feng,Wen, Hai-Shen,Li, Ji-Fang,Yu, Da-Hui,Ma, Rui-Qin,Shi, Dan,Mu, Wei-Jie,Zhang, Yuan-Qing,Hu, Jian,Liu, Miao,Han, Wei-Guo,Zhang, Jia-Nan,Wang, Qing-Qing,Yuan, Yu-Ren,Liu, Qun Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.4

Gonadotropin-releasing hormone receptor (GnRHR) gene is expressed at the anterior pituitary gland and plays a key role in gonad development. This study aimed to investigate molecular genetic characteristics of the GnRHR gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of GnRHR gene on sex steroid level in Japanese flounder (Paralichthys olivaceus). We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the GnRHR gene in 75 individuals. We identified three SNPs in the GnRHR gene: P1 locus (C759A and C830T) in the coding region of exon2 which were both linked together and P2 locus (G984T) in the coding region of exon3, which added a new transcript factor (ADR1) and a new methylation site (CG). Only C830T of P1 leads to amino acid changes Thr266Ile. Statistical analysis showed that P1 was significantly associated with $17{\beta}$-estradiol ($E_2$) level (p<0.01) and gonadosomatic index (GSI) (p<0.05). Individuals with genotype BB of P1 had significantly higher serum $E_2$ levels (p<0.01) and GSI (p<0.05) than those of genotype AA or AB. Another SNP, P2, synonymous mutation, was significantly associated with GSI (p<0.05). Individuals with genotype AB of P2 had significantly higher GSI (p<0.05) than that of genotype AA. In addition, there was a significant association between one diplotype based on three SNPs and reproductive traits. The genetic effects for both serum $E_2$ level and GSI of diplotype D4 were super diplotypes (p<0.05). These results suggest that the SNPs in Japanese Flounder GnRHR are associated with $E_2$ level and GSI.

Comparative Study between ZOOMit and Conventional Intravoxel Incoherent Motion MRI for Assessing Parotid Gland Abnormalities in Patients with Early- or Mid-Stage Sjögren’s Syndrome

Zhou Qing-Qing,Zhang Wei,Yu Yu-Sheng,Li Hong-Yan,Wei Liang,Li Xue-Song,He Zhen-Zhen,Zhang Hong 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.4

Objective: To compare the reproducibility and performance of quantitative metrics between ZOOMit and conventional intravoxel incoherent motion (IVIM) magnetic resonance imaging (MRI) in the diagnosis of early- and mid-stage Sjögren’s syndrome (SS). Materials and Methods: Twenty-two patients (mean age ± standard deviation, 52.0 ± 10.8 years; male:female, 2:20) with early- or mid-stage SS and 20 healthy controls (46.9 ± 14.6 years; male:female, 7:13) were prospectively enrolled in our study. ZOOMit IVIM and conventional IVIM MRI were performed simultaneously in all individuals using a 3T scanner. Quantitative IVIM parameters - including tissue diffusivity (D), pseudodiffusion coefficient (D*), and perfusion fraction (f) - inter- and intra-observer reproducibility in measuring these parameters, and their ability to distinguish patients with SS from healthy individuals were assessed and compared between ZOOMit IVIM and conventional IVIM methods, appropriately. MR gland nodular grade (MRG) was also examined. Results: Inter- and intra-observer reproducibility was better with ZOOMit imaging than with conventional IVIM imaging (ZOOMit vs. conventional, intraclass correlation coefficient of 0.897–0.941 vs. 0.667–0.782 for inter-observer reproducibility and 0.891–0.968 vs. 0.814–0.853 for intra-observer reproducibility). Significant differences in ZOOMit f, ZOOMit D*, conventional D*, and MRG between patients with SS and healthy individuals (all p < 0.05) were observed. ZOOMit D* outperformed conventional D* in diagnosing early- and mid-stage SS (area under receiver operating curve, 0.867 and 0.658, respectively; p = 0.002). The combination of ZOOMit D*, MRG, and ZOOMit f as a new diagnostic index for SS, increased diagnostic area under the curve to 0.961, which was higher than that of any single parameter (all p < 0.01). Conclusion: Considering its better reproducibility and performance, ZOOMit IVIM may be preferred over conventional IVIM MRI, and may subsequently improve the ability to diagnose early- and mid-stage SS.

Lysinibacillus tabacifolii sp. nov., a Novel Endophytic Bacterium Isolated from Nicotiana tabacum Leaves

Yan-Qing Duan,Song-Tao He,Qing-Qing Li,Ming-Feng Wang,Wen-Yuan Wang,Wei Zhe,Yong-Hong Cao,Ming-He Mo,Yu-Long Zhai,Wen-Jun Li 한국미생물학회 2013 The journal of microbiology Vol.51 No.3

A Gram-positive, catalase- and oxidase-positive, strictly aerobic, endospore-forming rod bacterium, designated K3514T, was isolated from the leaves of Nicotiana tabacum. The strain was able to grow at temperatures of 8–40°C, pH 5.0–10.0 and NaCl concentrations of 0–7%. The predominant quinones (>30%) of this strain were MK-7(H2) and MK-7. Phylogenetic analysis of 16S rRNA gene sequence showed that strain K3514T was affiliated to the genus Lysinibacillus, with its closest relatives being Lysinibacillus mangiferihumi (98.3% sequence similarity), Lysinibacillus sphaericus (97.9% sequence similarity), Lysinibacillus fusiformis (97.4% sequence similarity), and Lysinibacillus xylanilyticus (97.3% sequence similarity). However, low levels of DNA-DNA relatedness values suggested that the isolate was distinct from the other closest Lysinibacillus species. Additionally, based on analysis of morphological, physiological, and biochemical characteristics, the isolate could be differentiated from the closest known relatives. Therefore, based on polyphasic taxonomic data, the novel isolate likely represents a novel species, for which the name Lysinibacillus tabacifolii sp. nov. and the type strain K3514T (=KCTC 33042T =CCTCC AB 2012050T) are proposed.

Automatic 3D Model Acquisition for Unknown Objects Based on Hybrid Vision Technology

Wei Fang,Lianyu Zheng,BINGWEI HE,Qing Wang 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.3

Three-dimensional (3D) model acquisition is the process of building a 3D model of an object. But due to the limited field of view of the scanner, this task is mainly performed by taking several scans with human intervention. In order to make the 3D modeling process efficient, a novel automatic 3D modeling method for unknown objects based on hybrid vision technology in a binocular structured light system (BSLS) is proposed. Firstly, the limit visual vacuums of the BSLS are established, and they will be used to predict the unknown area with an acquired 2.5D range image. With the 2D intensity image acquired synchronously, the coarse boundary size is recovered from Shape from Shading, and it leads the prediction of the unknown area to be more precise. Based on the combination of the predicted contours, the next best viewpoint is determined with more unknown areas visible. The proposed method can be used to obtain t

Genetic Variation in PDCD6 and Susceptibility to Lung Cancer

He, Yan-Qi,Zhou, Bin,Shi, Shao-Qing,Zhang, Lin,Li, Wei-Min Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Lung cancer is the most common type of cancer and one of the leading causes of death in the world. Genetic factors play an important role in its development. PDCD6, the encoding gene for programmed cell death protein 6, may function as a tumor suppressor gene. Non-small cell lung cancer (NSCLC) contributes about 80% to newly histologically diagnosed lung cancer patients. To explore the relationship between PDCD6 and NSCLC, we examined two single nucleotide polymorphisms(rs3756712 G/T andrs4957014 G/T, both in the intron region) of the PDCD6gene.A hospital-based case-control study was carried out including 302 unrelated NSCLC patients and 306 healthy unrelated subjects. Significantly increased NSCLC risk was found to be associated with the T allele of rs4957014 (P=0.027, OR=0.760, 95%CI=0.596-0.970). The genotype and allele frequencies of rs3756712 did not shown any significant difference between NSCLC group and controls (P=0.327, OR=0.879, 95%CI=0.679-1.137). In conclusion, we firstly demonstrated the association between the PDCD6 gene and risk of NSCLC in a Chinese Han population.

Effect of metallic precursors on the thin film thickness and reaction resistances in the selenization process

Wei Liu,Jian-Guo Tian,Qing He,Feng-Yan Li,Chang-Jian Li,Yun Sun 한국물리학회 2011 Current Applied Physics Vol.11 No.3

In the selenization process, the sputtered metallic precursors transformed into CIGS thin films, which were investigated by novel in-situ resistance measurement. Simultaneously, the crystal phases and thicknesses of the selenized thin films at various selenization temperatures are obtained by XRD and XRF,respectively. According to the analysis of phase evolutions and reaction characteristics, it can be confirmed metallic In existed in the precursors will transform into the In―Se compound directly and then results in CIS formation as well as the thickness increase below 370 ℃. Otherwise, if alloy phases Cu ―In and Cu―Ga co-exist in the precursors, not CIS but CIGS will form above 470°, which will lead to both thickness and resistance increase in the corresponding temperature range. Consequently, it can be concluded the thickness increase are decided by the formation of CIS or CIGS, whereas the strong reaction peak in the temperature-resistance curves are caused only by stoichiometric CIGS.

Nanosheets of copolymerized ionic liquid-based polyelectrolyte complexes regulated at oil–water interface and their emulsification capability

He Zhang,Jun Bo Zhang,Qing Bo Meng,Wei Guo,Ming Yang,Shuyao Wu,Qiong Wu,Daliang Liu,Xi-Ming Song 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.97 No.-

The emulsification by polyelectrolyte complexes is easy to handle, and could be considered as an effectivestrategy for introducing functional materials to liquid–liquid interfaces, but the related studies are stillinsufficient. Herein, a kind of copolymerized ionic liquid (PIL-co-PVIm) which is synthesized from 1-vinyl-3-ethylimidazolium bromide and 1-vinylimidazole, are used as polycation to form polyelectrolytecomplexes type Pickering emulsifiers with sodium alginate, poly(sodium 4-styrenesulfonate) and poly(acrylic acid) sodium salt respectively as polyanion. For toluene-water emulsion systems andhydrophobic ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIm]PF6)-watersystem, the polyelectrolyte complexes formed by PIL-co-PVIm and sodium alginate or poly (sodium4-styrenesulfonate) showed best emulsification capability. The emulsions stabilized by the polyelectro-lyte complexes at stoichiometric ratio are relatively less stable compared to non-stoichiometriccomplexes, while PIL-co-PVIm without complexation has no emulsification capability. The polyelectro-lyte complexes based on homopolymerized PIL give an unsatisfied emulsification capability, while theemulsification performance could be remarkably improved by copolymerizing ionic liquids with theuncharged comonomer, but excessive contents of the uncharged unit make the polyelectrolytecomplexes less interface active. The sheet-like PECs are formed in aqueous dispersions, and theirthickness is remarkably reduced at oil–water interface after emulsification, approximately ranging from200 nm to 10 nm by manipulating the polyanion.

The effect of colored plastic films on the photosynthetic characteristics and content of active ingredients of Dysosma versipellis

Bing He,Yao Chen,Hua Zhang,Chunyan Xia,Qing Zhang,Wei Lin 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.4

Light intensity and quality affect photosynthesis, plant morphology, and the synthesis of primary and secondary metabolites. Dysosma versipellis (Hance) M. Cheng is an endangered species endemic to China, and a highly valued medicinal and ornamental plant. In this study, we discuss the effects of different light spectrums conferred by colored plastic films on photosynthesis and the contents of active ingredients of D. versipellis. D. versipellis plants were cultured for 90 days under white, red, yellow, or blue film. The blue film treatment generally increased the chlorophyll content, photosynthetic rate (Pn), maximal photochemical efficiency of PSII (Fv/Fm), actual photochemical efficiency of PSII (ФPSII), photochemical quenching (qP), and the podophyllotoxin content of the rhizomes. The blue film treatment also decreased the maximum net photosynthetic rate (Amax), apparent photosynthetic quantum efficiency (AQY), and podophyllotoxin contents of the stems. The yellow film treatment resulted in a decline of the Amax, AQY, Fv/Fm, ФPSII, qP, chlorophyll contents, and podophyllotoxin contents of the leaves and rhizomes; however, the light compensation point (LCP), light saturation point, and minimum fluorescence (Fo) were increased. There were no significant differences in chlorophyll content, Amax, LCP, AQY, Fv/Fm, ФPSII, or qP between the white and red film treatments. These results suggest that in D. versipellis, blue film treatments promote photosynthesis and the accumulation of podophyllotoxin, while yellow film treatments inhibit photosynthesis and the accumulation of podophyllotoxin.

The PR10 gene family is highly expressed in Lilium regale Wilson during Fusarium oxysporum f. sp. lilii infection

Hua He,Diqiu Liu,Nannan Zhang,Wei Zheng,Qing Han,Bo Ji,Feng Ge,Chaoyin Chen 한국유전학회 2014 Genes & Genomics Vol.36 No.4

Pathogenesis-related (PR) proteins play keyroles in plant responses to pathogens and abiotic stresses. In this study, nine novel PR genes were isolated from Liliumregale Wilson, which is a wild lily species of Chinawith high-level resistance to the soilborne fungal pathogenFusarium oxysporum f. sp. lilii, and homology analysisclassified them into the PR10 family. These novel LrPR10swere clustered together with PR10s from monocotyledonsin a phylogenetic tree, moreover, phylogenetic analysisdivided the nine LrPR10s into two groups. The main-chainconformation and folding patterns of the LrPR10s werehighly conserved with other plant PR10s. The expressionpatterns of the nine LrPR10s in L. regale during normaldevelopment were examined by QRT-PCR, and the transcriptionlevels of the LrPR10s were relatively high inroots. Furthermore, QRT-PCR analysis indicated that theexpression levels of LrPR10-1, LrPR10-2, LrPR10-5,LrPR10-6, and LrPR10-7 in L. regale roots were up-regulatedby two or more stress-related signaling moleculesincluding salicylic acid, jasmonic acid, ethylene, and H2O2,while the other four LrPR10s were repressed by these foursignaling molecules. In addition, five members of theLrPR10 gene family including LrPR10-2, LrPR10-4,LrPR10-5, LrPR10-6, LrPR10-7, and LrPR10-9 werestrongly induced by F. oxysporum in resistant L. regalecompared with the susceptible Lilium Oriental hybrid‘Siberia’. The other four LrPR10s were down-regulated byF. oxysporum infection. In summary, our results indicatethat the members of PR10 gene family are involved inL. regale defense responses against F. oxysporum f. sp. lilii.

Sensitivity of Plasmodium falciparum to Antimalarial Drugs in Hainan Island, China

Shan-Qing Wang,Guang-Ze Wang,Yu-Chun Li,Feng Meng,Shi-Gan Lin,Zhen-Hu Zhu,Ding-Wei Sun,Chang-Hua He,Xi-Min Hu,Jian-Wei Du 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.1