유색 보리 종실의 품종별 색소 특성

주완택,민경수,박노동 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4

유색보리 4품종과 보통보리 4품종 종실의 종피에 함유되어 있는 색소의 특성을 분석하여 해당작물의 신품종 육성시 우수계통 선발에 유용한 자료를 제공코자 하였다. 유색(검정)쌀보리인 Ab2231을 표준시료로 하여 실험한 결과 에탄올의 농도가 80%일 때 색소추출 효율이 양호하였다. 80% 에탄올에 1.0% 염산을 첨가한 것이 추출효율이 가장 좋았다. 추출시간에 따른 추출효율성을 조사한 결과 1시간 추출이면 충분하였다. 추출된 색소의 안정성을 유지하기 위하여 첨가시키는 산의 적정농도를 알아본 결과 1.0% HCl을 첨가한 농도에서 가장 낮은 색소량 감소율이 나타났다. 각 보리 품종 시료에서 추출한 색소의 흡광스펙트럼을 조사하였던 바, Ab2231의 그것은 535㎚에서 최대흡광파장을 나타내어 안토시아닌계 색소로 판단되었으며, 호계4호의 그것은 안토시아닌계 이외의 색소를 주로 함유하는 것으로 보였다. 한편, 추출된 색소의 특성에 따라 유색보리의 품종은 Ab2231와 모찌무기로 대표되는 쌀보리 그룹과 Lion과 호계4호로 대표되는 겉보리 그룹으로 분류되었다. The characteristics of the pigments extracted from grains of the colored barley varieties Ab2231. Mozzimugi, Lion, and Hogye 4 were studied. Extraction of the pigments from powder (80 mesh) of the barley grains was most efficient in 80% ethanol containing 1.0% HCI for 1 hr at 60℃. The pigments were also stable in 80% ethanol containing 1.0% HCI. Depending on the absorption spectra, maximal peak wavelength, and the ratio of A_(440)/Anu_(max), the varieties were separated into two groups, colored naked barley (Ab2231 and Mozzimugi) and colored covered barley (Lion and Hogye 4).

농업인 평생학습체계 구축을 위한 농업인교육 발전방안 연구

정철영,송병국,김진모,문택석,허영준,주대진,최수정,한승완 한국농업교육학회 2008 농업교육과 인적자원개발 Vol.40 No.2

이 연구의 목적은 우리나라 농업인의 평생학습체계 구축을 위한 농촌진흥기관의 역할을 정립하고, 한국농업대학의 농업인대학 운영 본부화 방안을 수립하는데 있다. 이를 위해 관련 문헌고찰, 농업인 및 농촌진흥기관의 농업인 교육 담당자들의 인식 및 요구분석, 전문가 자문 및 협의회 등의 방법을 활용하였으며, 이를 토대로 도출된 연구의 결과를 제시하면 다음과 같다. 첫째, 농촌진흥기관의 농업인교육관련 연계를 위해서는 한국농업대학이 농업인평생교육기관으로 평가인정을 받고, 전국의 농업인대학을 총체적으로 지원하는 역할을 수행하는 것이 바람직하다. 이때 지역의 농업기술센터는 한국농업대학과 협력적 관계를 구축하여 학점은행제 운영에 소요되는 교육 및 이력관리를 지원하는 역할을 수행하는 것이 필요하다. 둘째, 한국농업대학의 농업인대학 운영본부화를 위하여 평생학습원의 조직이 추가되는 것이 바람직하다. 이에 따라 평생학습원의 비전, 미션 및 전략이 제시되었으며, 조직의 기능 및 세부과제가 제시되었다. 셋째, 농업인교육의 학점은행제를 도입을 위한 체계를 설정한 후, 농업관련 기존 표준교육과정 분석, 농업인 요구분석, 전국 농업인대학 교육과정 분석, 기존 농업관련 전공 교육과정 분석, 전문가 검토 등의 과정을 거쳐서 '영농 전문학사'와 '농업 경영 학사'의 표준교육과정을 개발하였다. 넷째, 고졸미만 농업인을 위한 사전학습제도화를 위하여 농업인교육의 평가인정을 통한 방안과 One-Stop 평생학습모델을 통한 방안을 제시하였다. The purposes of this research were to assign the role of rural development organizations, and to establish the strategy for the making headquarter of farmer's university. The study was performed as follows: (1) analyzing the related literatures, documents, records review, (2) need analysis of farmers and members ig rural development organizations in terms of farmer's lifelong learning, (3) consulting experts. The following four findings are the results of this study: (1) Korean national agricultural college should be headquarter of farmer's universities and be approve for lifelong learning credit. Also, agricultural technology & extension centers should run educational program for the lifelong learning credit. (2) For the doing role of headquarter of farmer's university, division of lifelong learning should be add to department of technology training in Korea agricultural college. In relation with this, vision, mission and strategy of division of lifelong learning were suggested. (3) Steps for introduction of credit bank system of farmer education were suggested. Also standard curriculums of 'farming associate degree' and 'agricultural management bachelor's degree' were developed through analyzing existing standard curriculum related with agriculture, analyzing need of farmer, analyzing curriculum of farmer's university, analyzing curriculum of agriculture major. (4) For farmer who do not have high school graduate, two solutions were suggested which are approving farmer's education and one-stop lifelong learning model.

성인원외폐렴의 원인미생물에대한 전향적 다기관 연구 : 성인원외폐렴의 원인으로 세균의 역할을 중심으로

우준희,강재명,김양수,신완식,류진홍,최정현,김양리,정희진,어수택,박춘식,정문현,정기석,이찬주,류지소 대한감염학회 2001 감염 Vol.33 No.1

Background : Communite-acquired peumonia (CAP) is one of the leading causes of mortality and morbidity. Despite progress in diagnostic techniques and treatments, management of pneumonia remains challenging, because the precise etiology remains uncertain in as many as 49% of cases. The limitaions of identifying etiologic agents make it necessary to use empiric antibiotics in almost all patients, and furthermore emergence of antibiotic-resistant organisms pose difficulties to the selection of an empiric antibiotic regimen. For the optimal choice of empirical antibiotics, we should know the frequency of etiologic agents and antimicrobial resistance rates in the community. Methods : A prospective multicenter study of community-acquired pneumonia in Korea was carried out between May 1997 and April 2000. The microbiologic diagnosis was based on the results of sputum culture, blood culture and pleural culture. Results : Five hundred eighty eight cases of community-acquired peumonia in 562 patients admitted to the hospitals. The mean age was 59.9 with male predominance (58.3%), and 370 (63%) had underlyin gillness. The etiologic agents were identified in 38.3%, and the list of individual agents, in decreasing order, was Streprococcus pneumoniae (21.7%), Klebsiella pneumoniae (14.8%) Pseudomonas aeruginosa (9.8%), Staphylococcus aureus (9.5%), viridans group streptococci (5.7%), Enterobacter cloacae (4.2%), Hemophillus Influenza (3.8%), The rates of admission to the intensive care unit was 10.4%. The motality was 7.1%. Susceptible rates of S. pneumoniae to penicillin was 36.6% and showed multidrug resistant. Forty percents of S. aureus were methicillin-resistant S. aureus. K. penumoniae were susceptible to cephalosporin and quinolone. Conclusion : In Korea, S.pneumoniae is the most important agent causing community-acquired pneumonia. Susceptible rates of S. pneumoniae to penicillin was 36.6% and showed multidrug resistant. Gram negative bacteria such as K. pneumoniae, P. aeruginosa showed high incidence when compared with that of other countries. Most of them had underlying diseases including bronchiectasis and chronic obstructive pulmonary diseases. (Korean J Infect Dis 33:1∼7, 2001)

Comparison of optimal temperature and time conditions for highest a-glucosidase inhibitory activity from various of Korea mulberry teas

Ju, Wan-Taek,Kim, Hyun-Bok,Sung, Gyoo-Byung,Kim, Yong-Soon Korean Society of Sericultural Science 2016 International Journal of Industrial Entomology Vol.33 No.1

The influence of commonly used steeping times and temperatures about mulberry tea on a-glucosidase inhibitory activity were studied. The effects of product and preparation variables on the in-cup chemical composition of mulberry tea extracts is of interest because the appearance and taste characteristics and the possible health effects of a tea liquor arise from the chemical components extracted from the leaf during mulberry tea preparation. A comprehensive study was therefore undertaken to determine the contributions of product and provide a basic data for development of high quality mulberry tea products against diabetes. Specific eight mulberry tea were collected from each region of Korea (A-I) and when it was extracted on 85℃ for 2min, a-glucosidase activity was best for 98-102%. According to various temperature and time extraction methods, this study was carried out to optimize teamaking conditions for maximal DNJ extraction from Korean mulberry tea and and it would be of particular interest for people who drink mulberry tea to control blood glucose levels.

Mulberry popcorn disease occurrence in Korea region and development of integrative control method

( Wan-taek Ju ),( Hyun-bok Kim ),( Gyoo-byung Sung ),( Kwang-young Park ),( Yong-soon Kim ) 한국잠사학회 2016 International Journal of Industrial Entomology Vol.33 No.1

Mulberry fruits also have tremendous potential for providing various valuable industrial products of very high economic value for human beings. Nevertheless, through global warming, the popcorn disease caused by sclerotia forming fungi reduces the productivity of mulberry fruits in worldwide. So, in this study, we investigated damage ratio of mulberry popcorn disease in mulberry fruit production farm (Buan, Jeongueb, Sangju, Gochang in Korea). In Jeonbuk Buan, popcorn disease rate was the highest about 30%, on the other hand, in case of Gyungbuk Sangju and Jeonbuk Gochang, not damage. Also, we investigated about popcorn disease prevention by various of chemical treatment methods.

Anti-atopic Effects of the Silkworm Feces Extracts in the NC/Nga Mice

( Wan Taek Ju ),( Hyun Bok Kim ),( Kee Young Kim ),( Gyoo Byung Sung ),( Yong Soon Kim ) 한국잠사학회 2013 International Journal of Industrial Entomology Vol.27 No.2

This study was evaluated the antiatopic activity of silkworm feces extracts in DNCBinduced NC/Nga atopy mice. It was found that each level of IgE and histamine in blood was significantly decreased in the silkworm feces extracts treatment groups, compared with the DNCB-induced atopy control group. When the silkworm feces extracts was applied to the atopy mice, it could be observed that its skin recovered to normal condition with the skin surface being clean and smooth without any tissue. The results suggest that the application of silkworm feces extracts effect on atopic model through a inhibition of histamine emissions with reducing the levels of blood IgE in NC/Nga atopy mice.

Screening of Poly-γ-glutamic Acid (γ-PGA) Producing Bacteria from Environmental Soils

( Wan Taek Ju ),( Yong Su Song ),( Young Ju Kim ),( Woo Jin Jung ),( Ro Dong Park ) 한국키틴키토산학회 2013 한국키틴키토산학회지 Vol.18 No.3

Application of γ-PGA is limited due to the high cost associated with its production. Screening bacterial strains in order to find a more efficient producer is one of the platforms towards overcoming its limited usage. Over the 1500 colonies of bacteria were isolated from soil samples. From them, the 6 isolates, MJ80, CS4-29, HB-31, HB-31c, H1-19, and H1-07, were selected as candidates for γ-PGA production. Based on their morphological and biochemical properties and 16s rDNA sequences, MJ80 and CS4-29 were ultimately identified as Bacillus subtilis, H1-07 as B. thuringiensis, HB-31and HB-31c as B. anthracis, and H1-19 as B. sonorensis. The yield of γ-PGA ranged from 3.28 to 4.4%. It turned out that B. subtilis MJ80 and B. subtilis CS4-29 utilized both glutamic acid (GA) and soybean for γ-PGA production. B. thuringiensis H1-07 was identified as GA-dependent, while the other 3 strains were GA-independent. All strains produced high molecular weight γ-PGA over 200 kDa.

Quantitative analysis of rutin content using silkworm genetic resources

( Wan Taek Ju ),( Kee Young Kim ),( Gyoo Byung Sung ),( Yong Soon Kim ) 한국잠사학회 2015 International Journal of Industrial Entomology Vol.31 No.2

Rutin is an important bioflavonoid that is consumed in the daily diet. This study compared the functional components of rutin from various silkworm species using a gene database with those of rutin produced by silkworms selectively bred through cross-combinations. We made comparisons between the geographical origin and species of silkworm using a gene database and discovered that rutin activity was ranked in the following order by species, Chinese (C5)> miscellaneous varieties (Jamsaeng 1 Ho) >Japanese (Jam 115) > European (E58) >Korean (Sun 3 ho). However, rutin levels with respect to various genetic traits (blood color, silk color, and egg color) were consistent. In order to study rutin changes that occurred during the cross breeding of the silkworm gene, we bred cross-combinations utilizing Jam 115 and the 4051 silkworms. In conclusion, in order to provide information about the constituents of functional materials contained in silkworm powder, it is imperative that silkworm cross breeding occurs so that the database of functional materials extracted from silkworms will expand.

Flavonoids analysis about mulberry fruit of Korean mulberry cultivar, ‘Daeshim’

( Wan-taek Ju ),( O-chul Kwon ),( Yong-soon Kim ),( Hyun-bok Kim ),( Gyoo-byung Sung ),( Jong-gil Kim ) 한국잠사학회 2018 International Journal of Industrial Entomology Vol.37 No.2

Mulberry fruit is a new income product in Korea sericulture due to the increase of fruit consumption. However, flavonoids of Korean mulberry cultivar for fruit production did not reported yet. In this study, the typical mulberry cultivar, ‘Daeshim’ was analyzed using ultrahigh performance liquid chromatography coupled with diode array detection and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF/MS) technique for flavonoids analysis. Nine flavonoids were isolated and analyzed from Daeshim using UPLC-DAD-QTOF/ MS chromatogram. According to quantitative analysis, rutin (66.1 mg/100g DW) and quercetin 3-O-(6”-O-malonyl) glucoside (26.7 mg/100g DW) were abundant in mulberry fruit. Our results might be used as basic information for mulberry consumption.

Effect of the Nutritive Components of Mulberry Fruits From Two Cultivars Based on Irrigation Scheduling

( Wan-taek Ju ),( Sora Lee ),( Heon-woong Kim ),( You-young Jo ),( Hae-yong Kwon ) 한국환경농학회 2020 한국환경농학회지 Vol.39 No.4

BACKGROUND: The mulberry cultivars ‘Daeshim’ and ‘Shimgang’ were developed in RDA in 2014 and 2017, respectively. ‘Daeshim’ yields a fruit size bigger than that of other varieties and has a productivity of over 70%, whereas ‘Shimgang’ has a high yield and a special characterization against the mulberry popcorn disease. In our study, a compositional comparison of these popular cultivars in Korea was undertaken to explore the nutrient profiles of mulberry fruit and promote the development of the rich minerals and flavonoids in mulberry fruit as performing each other irrigation time. METHODS AND RESULTS: These two cultivars were collected from the Sericulture and Apiculture Division, RDA, in Korea to investigate their amounts, weights, minerals, and flavonoid content using each other instrument. After 6 h of irrigation treatment, the amount of fruit (kg/tree) from Daeshim and Shimgang increased by 17.5 and 15.2 kg/tree, respectively. The total flavonoid content from Daeshim and Shimgang was determined to be 132.9 mg and 36.3 mg, respectively, after the 6 h irrigation treatment. CONCLUSION: Appropriate irrigation treatment methods such as water scheduling and volume will help increase fruit quantities and farmer incomes. It would be interesting to conduct further in-depth research on these fruits so that consumers can benefit from them as a food additive.