Thermoplasma acidophilum의 20S Proteasome에 의한 ATPase-활성적 단백질분해에 관한 연구

우기민,장예진,조만희,김창세,김완종,조성호,이상한 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

The eukaryotic 26S proteasome is an ATP/ubiquitin-dependent proteolytic complex consisting of the 20S core particle and 19S ATPase complex. However, because of its complexity and unstable properties, this study was carried out to present more simple and stable model for the ATP-activated proteolytic complex in prokaryotes which can take the place of the eukaryotic 26S proteasome. For this purpose, recombinant Thermoplasma 20S proteasome (T20S) and Methanococcus MS4, a sequence homolog of one ATPase subunit in the 19S ATPase complex, were successfully isolated from Escherichia coli (E. coli). The α and β subunits of T20S expressed in E. coli could assemble for themselves, and showed the peptide-hydrolyzing activity. Whereas both T20S and R20S (the 20S complex from rabbit skeletal muscle) had the highest peptidase activity against Suc-LLVY-AMC, a good substrate for chymotrypsin-like peptidase activity, the specific activity of T20S was slightly lower than that of R20S. In addition, several reagents such as KCI, SDS, and ovalbumin were shown to have different effects on the peptidase activities between T20S and R20S. When the ATPase activity of the purified MS4 were assayed , the Km for ATP was about 0.5 mM, and casein could stimulate the activity more than 2 fold without the change in Km. This result implicates that protein-activated ATPase may induce the conformational change of casein, and therefore suggests that MS4 ATPase may activate the proteolytic activity of the 20S proteasome via accelerating the recognition and translocation of the protein substrates.

췌십이지장절제술 후 발생한 지속적인 구토증과 Wernicke 뇌병증

박우영,김성완,이삼연,신일선,김재민,박기형,양수진,윤진상 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.3

Wernicke encephalopathy is an acute neurologic disorder attributable to thiamine (vitamin B1) deficiency. We report the case of a 61-year-old female patient who presented Wernicke encephalopathy after surgery for pancreatic head cancer. From the ninth postoperative day, she had suffered from nausea and vomiting and had difficulties ingesting food, she was given total parenteral nutrition (TPN), but lacked adequate vitamin (thiamine) supplementation. After 28 days, she developed ataxia, ophthalmoplegia, and mental confusion. The magnetic resonance image showed pathologic changes in the medial thalamus,periaqueductal gray matter, medulla and mamillary bodies. The blood level of thiamine was very low. After intravenous and oral supplementation of thiamine (200 mg/day), consciousness was soon normalized and neurologic symptoms have gradually been improving. Nausea and vomiting disappeared after administration of a low dose of mirtazapine (7.5 mg/day). We emphasize the importance of thiamine supplementation to the patients who suffer from vomiting which hinders them from taking food and who require prolonged TPN.

Escherichia coli 패혈증 환자에 합병된 대칭적 하지 말단 괴사증 1예

남해성,유진홍,권순석,민준기,조현선,박민경,심병주,남유정,이지인,김진수,길욱현,조근종,신완식 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

We have encountered a rare case of symmetrical peripheral gangrene complicating Escherichia coli sepsis in a 47-years-old male. He was successfully treated with antibiotics, anticoagulants, and vasodilator. To our knowledge, this is the first report on symmetrical peripheral gangrene complicating E. coli sepsis in Korea.

예비창업자의 창업가 학습, 사회적 자본과 창업지원제도가 창업의지와 행동에 미치는 영향 : 중국 대학생 창업을 중심으로

민완기(Wan-Kee Min),김장훈(Jang-Hoon Kim),진가(Ke Chen) 한국무역연구원 2020 무역연구 Vol.16 No.1

Purpose- The purpose of this study was to investigate the effect of pre-founder’s entrepreneur’s learning, social capital, and start-up support programs on the will of start-up and the start-up behavior in Chinese university students. Design/methodology/approach- For the purpose of this study, we examined two variables of experiential learning and cognitive learning as variables of entrepreneur’s learning. As variables of social capital, we examine three variables of norm, network, and trust. As variables of start-up support programs, we examined two variables of government start-up support programs and school start-up support programs. The data for this study were collected from the students of the founding clubs of five universities and students who participated in the start-up activities in Anhui province, China. The online survey was used as the data collection tool, and the collected data was analyzed using correlation analysis and regression analysis. Findings - First, entrepreneur’s learning, social capital excluding trust and start-up support programs have a positive effect (+) on the will of start-up. Two, it is shown that pre - founder ’s willingness to start up affects the start - up behavior positively (+). Three, entrepreneur’s learning, social capital, and start-up support programs have positive effects (+) on the start-up behavior through the will of start-up. The theoretical and policy implications of the findings of this study are discussed and suggestions for future research are suggested. Research implications or Originality- Theoretical and policy implications in this study are discussed and suggested for future research. First, we identified key antecedents to influence the will of would-be start-up, and examined the relationship between the will of start-up and the behavior of preliminary founders. Second, our findings suggest that entrepreneurial learning and institutional supporting program will be key factors to advance the will of behavior and to establish start-up company in the future.

대학교, 연구소 및 테크노파크 기업의 지속가능성에 관한 비교연구

민완기 ( Min Wan-kee ),김장훈 ( Kim Janghoon ),최준 ( Choi Jun ) 충북대학교 산업경영연구소 2019 産業과 經營 Vol.32 No.1

본 연구는 스타트업의 지속적 성장에 관심을 갖고 연구에 착수하였다. 우선 창업 후 성장통을 극복하는데 영향을 미치는 요인을 도출하는 것에 초점을 맞췄다. 다음으로 조직을 성장으로 이끄는 동인이 있다면 그 동인을 도출하는데 주력하였다. 이를 위해 대학교 창업보육센터, 국책연구소 그리고 테크노파크에서 창업한 기업을 선정하였다. 즉 퓨어시스, 콜마BNH 및 선진이엔지를 대상으로 각각 사례연구를 수행하였다. 분석결과에 따르면 기업 특유의 독점적 우위로서 기술역량을 토대로 기술창업을 단행한 것으로 나타났다. 달리 말해 창업자이자 경영자는 동종분야에 대한 지식과 기술을 축적한 전문성을 바탕으로 창업을 주도하고 경영활동을 수행하였다. 다음으로 콜마BNH처럼 원활한 자본조달이 성장의 밑거름이 된다는 점에서 지배구조의 변화를 필요로 한다. 더욱이 창업보육센터는 교육과 육성이 시급한 반면, 연구소 및 테크노파크 기업은 자금 및 경영지원이 중요한 요인으로 보았다. 결국 기업의 유형과 성장단계에 따라 성공요인이 달라지는 것을 볼 때 맞춤형 지원과 촉진책이 중요할 것이다. 비록 복수기업 사례연구에도 불구하고 일반화 가능성을 제고할 수 있는 후속연구가 요구된다. This study starts from research question concerning the sustainability of start-up or spin-off company. First of all, it focused on elaborating key factors to overcome a kind of ‘death valley’. Next, we tried to identify some other factors to enhance a firm’s rapid growth. For this reason, business incubator, innopolis research institute spin-off and techno-park company were selected as representative sample. That is, this research conducted in-dept case study on Puresys, Kolmar BNH and Sunjin Eng. respectively. According to case analysis, technological resource is recognized as firm-specific advantage to start-up or spin-off firm under an early stage. In addition, chief executive as founder who is specialized in related technical field is basic factor to play key role to launch its own business. Like Kolmar BNH, change of corporate governance will be necessary to sustain rapid growth. Moreover, business incubator calls for education, information access as incubating mechanism, while capital financing, managerial skills like marketing or strategy are required to accelerate. Most but not least, incubating or accelerating programs should be tailored to start-up and spin-off company in terms of location and stage by stage. Even though multiple cases, follow-up research are indispensible to generalize result of present study.

시스템반도체 산업기반조성사업의 비경제적 성과 분석

민완기 ( Wan Kee Min ),오완근 ( Wan Keun Oh ) 서강대학교 시장경제연구소(구 서강대학교 경제연구소) 2011 시장경제연구 Vol.40 No.2

오늘날 공공지출의 성과기반관리가 확산되면서 국내에서도 국가연구개발사업의 성과 분석이 진행되고 있지만, 대부분의 연구는 경제적 성과를 분석하는데 초점을 맞추고 있다. 즉 국가연구개발사업이 해당산업 및 관련산업에서 실현하는 매출액, 이윤, 생산유발액 등에 관한 분석이 주류를 이루었다. 본 연구는 국가연구개발사업에 의한 차세대 기반기술 확보, 사회구성원 삶의 질 향상 등 시장에서 실현되지 않고 측정하기 어려운 비경제적 성과를 분석하기 위한 탐색적 시도이며, 구체적인 분석대상은 시스템반도체 산업기반조성사업이다. 관련기업 CEO 들과의 인터뷰를 통하여 시스템반도체 산업기반조성사업의 비경제적 성과의 속성으로서 차세대 기반기술 확보, IT 산업 고도화, 전문인력양성, 기업 Start-up의 네 가지가 파악되었다. 이들을 비시장재 가치평가의 대표적인 분석방법론인 MAUT를 이용하여 평가한 결과, 1997년부터 2006년까지 시스템반도체 산업기반조성사업의 비경제적 성과의 화폐가치는 약 2,102억원에서 약 4,890억원으로 평가되었다. 동 금액은 1997년부터 2006년까지 1,031억원의 사업비용 대비 약 0.56~1.3배에 해당하는 금액이다. As the assessment of public expenditure outcomes has been expanded today, the assessment of national R&D projects is also being conducted, most of whom analyzes their economic effects only. Specifically, national R&D projects tend to focus on the analysis of sales, profit, production-induced amount of relevant industries mainly. In this situation, this study intends to explore an analysis of immeasurable and implicit non-economic effects in system IC industry infrastructure formation, such as the gain of fundamental technology of next generation, quality improvement of members in society, etc. Interviews with CEO of relevant companies are facilitated and four attributes are identified, including the gain of fundamental technology of next generation, advancement of IT industry, training of expertise, start-up of new enterprises. Data is analyzed by using MAUT which is a representative analysis method of non-market value assessment. The findings of this study show that the value of non-economic effects in system IC industry infrastructure formation from year 1997 to year 2006 is evaluated as to reach approximately 210 Billion Won to 489 Billion Won. This amount can be considered 0.56 to 1.3 times more than actual expenditures of 130 Billion Won during the same period.

국내 IT SoC산업의 기술추격

민완기(Min Wan-Kee),오완근(Oh Wan-Keun) 한국기술혁신학회 2006 기술혁신학회지 Vol.9 No.4

본 연구는 기술추격론 및 산업혁신체제론에 의거해서 국내 IT SoC산업의 기술추격을 검토했다. 국내 IT SoC산업은 많은 중소ㆍ벤처 팹리스업체들의 출현에 의한 다양성 창조 및 일부 팹리스업체의 급성장에 따른 선택의 과정을 거쳐 급속한 기술추격 과정을 밟아왔다. 이 과정에서 국내 팹리스업체들은 틈새시장을 중심으로 한 경로개척형 기술추격을 수행해 왔다. 현재 국내 IT SoC산업은 많은 문제점을 가지고 있지만, 산업혁신체제의 공진화를 달성하려면 무엇보다도 기업간 협력네트워크 구축이 선결과제이다. 기업간 협력네트워크는 시장의 힘에 의해 자동적으로 해결되기 어려운 시스템실패의 한 형태이기 때문에 이를 해결하기 위한 정부정책이 요망된다. This paper aims at examining technological catching-up in the Korean IT SoC industry using the theories of technological catching-up and sectoral system of innovation. The Korean IT SoC industry has accomplished rapid technological catching-up by the process of variety generation and selection. In the process, Korean fabless ventures have pursued the technological path-creative pattern in niche markets. But the cooperative networks among various firms are prerequisite for the coevolution of sectoral innovation system.

Microbial Inhibition and Antioxidation of the Extracts from Tetragonia tetragonioides

Min A Lee,Hye Jung Choi,Jum Soon Kang,Young Guen Lee,Dong Wan Kim,Ja Young Moon,Jeong Uck Park,Yong Kee Jeong,Woo Hong Joo 한국생명과학회 2006 한국생명과학회 심포지움 Vol.47 No.-

Electrical and Optical Characteristics in Organic Electroluminescent Devices with Different Materials for Electron Injection

Min-Jeong Cho,Wan-Ji Choi,Min-Su Lim,Cheol-Hyun Park,Jeong-Gu Lee,Kee-Joe Lim,Soo-Gil Park,Hyun-Hoo Kim 대한전기학회 2001 KIEE International Transactions on Electrophysics Vol.11C No.2

An Analysis of Relationships between IT Part Imports and IT Product Exports in Korea

( Wan Keun Oh ),( Wan Kee Min ),( Hoe Il Kang ) 한국EU학회 2008 Asia-Pacific Journal of EU Studies Vol.6 No.1

As imports of parts and materials in the IT industry in Korea have recently increased, a great deal of research has reported the current status of Korean imports and suggested new approaches to enhance the domestic IT industry. However, there have been lack of empirical studies that analyze a direct relationship between IT part imports and IT product exports. Therefore, this study conducts a time series analysis to examine such a relationship. Findings indicate that there is a significant correlation between those imports and exports. A cross correlation analysis demonstrated the two variables are coincident. The error correction model showed that the long run elasticity of IT part imports to IT product exports is 0.42.