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Engineering and Visualization of Bacteria for Targeting Infarcted Myocardium
Le, Uyenchi N,Kim, Hyung-Seok,Kwon, Jin-Sook,Kim, Mi Yeon,Nguyen, Vu H,Jiang, Sheng Nan,Lee, Byeong-Il,Hong, Yeongjin,Shin, Myung Geun,Rhee, Joon Haeng,Bom, Hee-Seung,Ahn, Youngkeun,Gambhir, Sanjiv S Elsevier Science B.V., Amsterdam 2011 Molecular therapy Vol.19 No.5
MIN, Jung-Joon,AHN, Youngkeun,MOON, Sungmin,Sook KIM, Yong,Eun PARK, Jong,Mi KIM, Sung,N. LE, Uyenchi,C. WU, Joseph,Yeon JOO, Soo,Hwa HONG, Moon,Hwan YANG, Deok,Ho JEONG, Myung,Hun SONG, Chang,Hyeok J Japanese Society of Nuclear Medicine 2006 Annals of nuclear medicine Vol.20 No.3
<P>Objective: The conventional method for the analysis of myocardial cell transplantation depends on postmortem histology. Here, we have sought to demonstrate the feasibility of a longitudinal monitoring of transplanted cell survival in living animals, accomplished with optical imaging techniques and pharmacological interventions. Methods: Human cord blood (50 ml) was donated with parental consent. After getting cord blood derived mesenchymal stem cells (CBMSCs), cellswere transfected (MOI = 100) overnight with adenovirus encoding firefly luciferase gene (Ad- CMV-Fluc). Our experimental Sprague-Dawley rats (n = 12) were given intramyocardial injections containing 1 × <TEX>$10^6$</TEX> CBMSCs, which had been made to express the firefly luciferase (Fluc) reporter gene. Optical bioluminescence imaging was then conducted using a cooled charged-coupled device (CCD) camera (Xenogen), beginning on the day after the transplantation (day 1). Groups of mice were intraperitoneally injected with cyclosporine (5 mg/kg) or tacrolimus (1 mg/kg), in an attempt to determine the degree to which cell survival had been prolonged, and these values were then compared with the cell survival values of the negative control group. The presence of transplantedCBMSCs on in vivo images confirmed by in situ hybridization for human specific Alu in the myocardium. Results: Cardiac bioluminescence signals were determined to be present for 6 days after transplantation: day 1 (97000 ± 9100 × 105 p/s/㎠/sr), day 3 (9600 ± 1110 p/s/㎠/sr), and day 5 (3200 ± 550 p/s/㎠/sr). The six mice that received either cyclosporine or tacrolimus displayed cardiac bioluminescence signals for a period of 8 days after transplantation. We observed significant differences between the treated group and the non-treated group, beginning on day 3 (tacrolimus;26500 ± 4340 p/s/㎠/sr, cyclosporine;27200 ± 3340 p/s/㎠/sr, non-treated;9630 ± 1180 p/s/㎠/sr, p < 0.01), and persisting until day 7 (tacrolimus;12500 ± 2946 p/s/㎠/sr, cyclosporine;7310 ± 1258 p/s/㎠/sr, non-treated;2460 ± 160 p/s/㎠/sr, p < 0.01). The humanderived CBMSCs were detected in the myocardium 3 days after transplantation by in situ hybridization. Conclusions: The locations, magnitude, and survival duration of the CBMSCs were noninvasively monitored with a bioluminescence optical imaging system. We determined that optical molecular imaging expedites the fast throughput screening of pharmaceutical agents, allowing for the noninvasive tracking of cell survival within animals. In rat cardiac CBMSC transplant models, transient immunosuppressive treatment with tacrolimus or yclosporine was shown to improve donor cell survival."</P>