Detection of pathogenic microorganisms using biosensor based on multi-walled carbon nanotubes dispersed in DNA solution

Nguyen Thi Thuy,Phuong Dinh Tam,Mai Anh Tuan,Anh-Tuan Le,Le Thi Tam,Vu Van Thu,Nguyen Van Hieu,Nguyen Duc Chien 한국물리학회 2012 Current Applied Physics Vol.12 No.6

The present paper introduces a facile and cost-effective route for the direct dispersion of multi-walled carbon nanotubes (MWCNTs) in DNA solution. Their application in detecting Escherichia coli O157:H7using DNA biosensorwas demonstrated. The dispersion state of theMWCNTswas characterized via UVeVis spectroscopy, transmission electron microscopy, and field emission scanning electron microscopy. The interaction between DNA sequence and the MWCNTs was investigated using Raman spectroscopy and Fourier transform infrared spectroscopy. As-obtainedMWCNT solutionwas used in the preparation of DNA sensor. Results revealed that the developed DNA sensor can detect a DNA target as low as 1 nM in a buffer solution. The sensitivity of the DNA sensor reached approximately 0.19 nM/mV. The effect of dispersion parameters, including pH values, DNA concentration, ion strength, and sonication time, on sensor response was also studied. TheDNA sensor can respondwell to 120 min of sonication time, a pH value of 9, and 20 mM of DNA sequence concentration. The results of the present study showed a potential application of the DNA sensor in the detection of Escherichia coli O157:H7.

A Comparative Study on the Adipogenic and Myogenic Capacity of Muscle Satellite Cells, and Meat Quality Characteristics between Hanwoo and Vietnamese Yellow Steers

Nguyen Thu Uyen,Dao Van Cuong,Pham Dieu Thuy,Luu Hong Son,Nguyen Thi Ngan,Nguyen Hung Quang,Nguyen Duc Tuan,In-ho Hwang 한국축산식품학회 2023 한국축산식품학회지 Vol.43 No.4

Myogenesis and adipogenesis are the important processes determining the muscle growth and fat accumulation livestock, which ultimately affecting their meat quality. Hanwoo is a popular breed and its meat has been exported to other countries. The objective of this study was to compare the myogenesis and adipogenesis properties in satellite cells, and meat quality between Hanwoo and Vietnamese yellow cattle (VYC). Same 28-months old Hanwoo (body weight: 728±45 kg) and VYC (body weight: 285±36 kg) steers (n=10 per breed) were used. Immediately after slaughter, tissue samples were collected from longissimus lumborum (LL) muscles for satellite cells isolation and assays. After 24 h post-mortem, LL muscles from left carcass sides were collected for meat quality analysis. Under the same in vitro culture condition, the proliferation rate was higher in Hanwoo compared to VYC (p<0.05). Fusion index was almost 3 times greater in Hanwoo (42.17%), compared with VYC (14.93%; p<0.05). The expressions of myogenesis (myogenic factor 5, myogenic differentiation 1, myogenin, and myogenic factor 6)- and adipogenesis (peroxisome proliferator-activated receptor gamma)-regulating genes, and triglyceride content were higher in Hanwoo, compared with VYC (p<0.05). Hanwoo beef had a higher intramuscular fat and total monounsaturated fatty acids contents than VYC beef (p<0.05). Whilst, VYC meat had a higher CIE a* and total polyunsaturated fatty acids content (p<0.05). Overall, there was a significant difference in the in vitro culture characteristics and genes expression of satellite cells, and meat quality between the Hanwoo and VYC.

Dynamic response and vibration of composite double curved shallow shells with negative Poisson's ratio in auxetic honeycombs core layer on elastic foundations subjected to blast and damping loads

Duc, Nguyen Dinh,Seung-Eock, Kim,Cong, Pham Hong,Anh, Nguyen Tuan,Khoa, Nguyen Dinh Elsevier 2017 International journal of mechanical sciences Vol.133 No.-

<P><B>Abstract</B></P> <P>The purpose of the present study is to investigate dynamic response and vibration of composite double curved shallow shells with negative Poisson's ratios in auxetic honeycombs core layer on elastic foundations subjected to blast and damping loads using analytical solution. This study considers composite double curved shallow shells with auxetic core which have three layers in which the top and bottom outer skins are isotropic aluminum materials; the central layer has honeycomb structure using the same aluminum material. Based on the first order shear deformation theory (FSDT) with the geometrical nonlinear in von Karman and using Airy stress functions method, Galerkin method and the fourth-order Runge–Kutta method, the resulting equations are solved to obtain expressions for nonlinear motion equations. The effects of geometrical parameters, material properties, elastic foundations Winkler and Pasternak, the nonlinear dynamic analysis and vibration of double curved shallow shells with negative Poisson's ratios in auxetic honeycombs core layer are studied.</P> <P><B>Highlights</B></P> <P> <UL> <LI> To investigate dynamic response and vibration of composite double curved shallow shells by using analytical solution. </LI> <LI> The composite shells have the central auxetic core layer—honeycomb structures with negative Poisson's ratio. </LI> <LI> Based on the first order shear deformation theory (FSDT). </LI> <LI> Used airy stress functions, Galerkin method and fourth-order Runge–Kutta method. </LI> <LI> The effects of geometrical parameters, material properties, elastic Winkler and Pasternak foundations, mechanical and blast loads are studied. </LI> </UL> </P>

Efficient Forest Fire Detection using Rule-Based Multi-color Space and Correlation Coefficient for Application in Unmanned Aerial Vehicles

Nguyen Duc Anh,Pham Van Thanh,Doan Tu Lap,Nguyen Tuan Khai,Tran Van An,Tran Duc Tan,Nguyen Huu An,Dang Nhu Dinh 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.2

Forest fires inflict great losses of human lives and serious damages to ecological systems. Hence, numerous fire detection methods have been proposed, one of which is fire detection based on sensors. However, these methods reveal several limitations when applied in large spaces like forests such as high cost, high level of false alarm, limited battery capacity, and other problems. In this research, we propose a novel forest fire detection method based on image processing and correlation coefficient. Firstly, two fire detection conditions are applied in RGB color space to distinguish between fire pixels and the background. Secondly, the image is converted from RGB to YCbCr color space with two fire detection conditions being applied in this color space. Finally, the correlation coefficient is used to distinguish between fires and objects with fire-like colors. Our proposed algorithm is tested and evaluated on eleven fire and non-fire videos collected from the internet and achieves up to 95.87% and 97.89% of F-score and accuracy respectively in performance evaluation.

A facile thermal evaporation route for large-area synthesis of tin oxide nanowires: Characterizations and their use for liquid petroleum gas sensor

Nguyen Van Hieu,Le Thi Ngoc Loan,Nguyen Duc Khoang,Nguyen Tuan Minh,Do Thanh Viet,Do Cong Minh,Tran Trung,Nguyen Duc Chien 한국물리학회 2010 Current Applied Physics Vol.10 No.2

In this paper, a very simple procedure was presented for the reproducible synthesis of large-area SnO2nanowires (NWs) on a silicon substrate by evaporating Sn powders at temperatures of 700, 750, and 800 ℃. As-obtained SnO2 NWs were characterized by field emission scanning electron microscopy (FESEM),transmission electron microscopy (TEM), X-ray diffraction (XRD), and Raman spectroscopy. They revealed that the morphology of the NWs is affected by growth temperature and the SnO2 NWs are single-crystalline tetragonal. The band gap of the NWs is in the range of 4.2–4.3 eV as determined from UV/visible absorption. The NWs show stable photoluminescence with an emission peak centered at around 620 nm at room-temperature. The sensors fabricated from the SnO2 NWs synthesized at 700 ℃ exhibited good response to LPG (liquefied petroleum gas) at an operating temperature of 400 ℃.

An Adaptive Multi-Layer Block Data-Hiding Algorithm that uses Edge Areas of Gray-Scale Images

Tuan Duc Nguyen,Somjit Arch-int,Ngamnij Arch-int 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.6

Embedding data into smooth regions introduces stego-images with poor security and visual quality. Edge adaptive steganography, in which the flat regions are not employed to carry a message at low embedding rates, was proposed. However, for the high embedding rates, smooth regions are contaminated to hide a secret message. In this paper, we present an adaptive multi-layer block data-hiding (MBDH) algorithm, in which the embedding regions are adaptively selected according to the number of the secret message bits and the texture characteristic of a cover-image. Via employing the MBDH algorithm, more secret message bits are embedded into the sharp regions. Therefore, the smooth regions are not used, even at high embedding rates. Furthermore, most of edge adaptive steganography algorithms have a limited capacity when the smooth regions are not employed in data hiding. The proposed scheme solves this issue when it can embed more secret bits into the selected regions while the perceptual quality of stego-images is still maintained. The experimental results were evaluated on 10,000 natural gray-scale images. The visual attack, targeted steganalysis, and universal steganalysis are employed to examine the performance of the proposed scheme. The results show that the new scheme significantly overcomes the previous edge-based approaches and least significant bit (LSB) based methods in term of security and visual quality.

Targeted and controlled drug deliveryl system loading artersunate for effective chemotherapy on CD44 overxpressing cancer cells

( Tuan Hiep Tran ),( Tuan Duc Nguyen ),( Han Van Nguyen ),( Hanh Thuy Nguyen ),( Jong Oh Kim ),( Chul Soon Yong ),( Chien Ngoc Nguyen ) 영남대학교 약품개발연구소 2016 영남대학교 약품개발연구소 연구업적집 Vol.26 No.-

Polyro.t-lactic-co-glycolic acid) (PLGA) nanoparticles with negative surface charge were reversed to positive by cationic surfactant-DDAB before being coated with an anionic polymer, hyaluronic acid, to improve their site-specific intracellular delivery against CD44 receptor overexpressing cancer cells. Incorporating artesunate (ART)-a promising anticancer drug into PLGA/HA nanoparticles, is expected not only to overcome its poor aqueous solubility and stability but also enhance the activities. The obtained particles were characterized by dynamic light scattering, zeta potential measurements, and transmission electron microscopy (TEM). Cancer cell internalization of the NPs was evaluated by flow cytometry and cytotoxicity of the NPs was tested by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay. PLGA/HA nanoparticles showed greater extent of cellular uptake to SCC-7 and MCF-7 cells, indicating their affinity with CD44 receptor-mediated endocytosis. Almost 60 % of ART was released into the outer media after 48 h. In vitro fluorescence sorting demonstrated that PLGA/HA had highly efficient targeting and accumulation into CD44 receptor overexpression cells. The significant reduction in cell viability as well as greater induction of apoptosis suggested a potential in anticancer therapy of ART loaded PLGA/HA.

Development and Evaluation of Artesunate-Loaded Chitosan-Coated Lipid Nanocapsule as a potential Drug Delivery System Against Breast Cancer

( Tuan Hiep Tran ),( Tuan Duc Nguyen ),( Bijay Kumar Poudel ),( Hanh Thuy Nguyeon ),( Jong Oh Kim ),( Chul Soon Yong ),( Chien Ngoc Nguyen ) 영남대학교 약품개발연구소 2016 영남대학교 약품개발연구소 연구업적집 Vol.26 No.-

Artesunate (ART)-a well-known hydrophobic anti-malarial agent was incorporated in a polymer-lipid hybrid nanocolloidal system for anti-cancer therapeulic, The lipid negatively charged nanocmulsion was formulated by modified hot homogenization method then covered with positively charged chitosan via clectroslatic interaction to obtain chitosan-coated lipid nanocapsule (ART-CLN). Physical properties of the system were characterized in terms of size, charge morphology, drug loading capacity, and physicca stale, In addition, anti-cancer activities were confirmed by conductiong MTT assay for ART and ART-CLN, on different cancer cell lines. Obtained ART_CLN after coating chilosan revelated positive charge (13.2±0.87 mV), small particle size (160.9±3.5 nm), and spherical shape. High drug entrapment cfficiency (95.49±1.13%) and sustaubed rekease pattern were obscrved. Moreover, the good cellular uptake was recorded by flow cytomctry as well as confocal image. Finally, ART-CLN Exhibited stronger anti-cancer activity than free ART on breast cancer cell lines (MCF-7, MDA-MB-231). These results suggested that by lading ART into lipid core of polymer lipid hybrid carrier, the activity and physical stability of ART can be significantly increased for cancer chemotherapy.

The complete mitochondrial genome sequence of the indigenous I pig (Sus scrofa) in Vietnam

Hieu Duc Nguyen,Tuan Anh Bui,Phuong Thanh Nguyen,Oanh Thi Phuong Kim,Thuy Thi Bich Vo 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.7

Objective: The I pig is a long nurtured longstanding breed in Vietnam, and contains excellent indigenous genetic resources. However, after 1970s, I pig breeds have become a small population because of decreasing farming areas and increasing pressure from foreign breeds with a high growth rate. Thus, there is now the risk of the disappearance of the I pigs breed. The aim of this study was to focus on classifying and identifying the I pig genetic origin and supplying molecular makers for conservation activities. Methods: This study sequenced the complete mitochondrial genome and used the sequencing result to analyze the phylogenetic relationship of I pig with Asian and European domestic pigs and wild boars. The full sequence was annotated and predicted the secondary tRNA. Results: The total length of I pig mitochondrial genome (accession number KX094894) was 16,731 base pairs, comprised two rRNA (12S and 16S), 22 tRNA and 13 mRNA genes. The annotation structures were not different from other pig breeds. Some component indexes as AT content, GC, and AT skew were counted, in which AT content (60.09%) was smaller than other pigs. We built the phylogenetic trees from full sequence and D loop sequence using Bayesian method. The result showed that I pig, Banna mini, wild boar (WB) Vietnam and WB Hainan or WB Korea, WB Japan were a cluster. They were a group within the Asian clade distinct from Chinese pigs and other Asian breeds in both phylogenetic trees (0.0004 and 0.0057, respectively). Conclusion: These results were similar to previous phylogenic study in Vietnamese pig and showed the genetic distinctness of I pig with other Asian domestic pigs.

Effect of Co-Doping and Tri-Doping with Transition Metals and a Nonmetal on Photocatalytic Activity in Visible Light of TiO2 Thin Film

Hang Nguyen Thai Phung,Van Nguyen Khanh Tran,Phuong Ai Duong,Hung Vu Tuan Le,Nguyen Duc Truong 한국물리학회 2017 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.70 No.11

Mono, co- and tri-doped TiO2 thin films with the transition metals (vanadium and/or chrominium) and a nonmetal (nitrogen) have been fabricated by sol-gel method. X-ray diffraction results clearly reveal anatase crystal structure for all obtained samples and doping with any dopants doesn’t change the anatase phase of TiO2. Compared to TiO2, three types of doped TiO2 thin films exhibit a red-shift in the absorption edge and have much better photocatalytic properties for methylene blue degradation in visible light region. The maximum visible-photocatalytic performance was achieved for tri-doped TiO2 sample. The mechanism for enhancing visible-photocatalytic activity of co-doped and tri-doped TiO2 thin films was also examined.