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RISS 인기검색어
- 검색키워드
- 저자 : ( Tsung-yan Tsai ) (검색결과 3 건)
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The Influence of PMMA Bone Cements on the Osteoblasts and Osteoclasts
( Kai-chen Hu ),( Tsung-yan Tsai ),( Chu-wen Shao ),( Hsin-i Chang ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
Due to aging, deficiency of vitamins and minerals, a prior accident or injury, the bones in our body become weak and fragile and so may cause serious defect of hard bone tissues. In orthopaedic surgery, Polymethylmethacrylate (PMMA) bone cements are used to refill and remodel bone defect. PMMA bone cements are formulated by mixing the powder (polymer, PMMA) and the liquid (monomer, methyl methacrylate). During the process, the dimethyl-p-toluidine in the liquid activates the benzoyl peroxide catalyst in the powder and this initiates the polymerization of the monomer, which then binds together PMMA granules. However, PMMA bone cements have displayed cytotoxicity to bone tissue in clinic. In present study, we used PMMA with different molecular weights (Mw:1,5000, 12,0000, 35,0000 and 99,6000 dalton) to prepare 4 types of bone cements for studying the effect of molecular weight of polymeric additives. Chemical eluates were extracted from PMMA bone cements and examined for their bioactive effects on 7F2 osteoblast-like cells and Raw 264.7 microphages. Results demonstrated that PMMA bone cements and eluates could inhibit the differentiation and mineralization behavior of 7F2 osteoblast-like cells after the cytotoxicity of PMMA bone cements and elutes were no longer evident. Moreover, bone cements prepared with high molecular weight PMMA (MW: 99,6000 dalton) had less suppressive effect on cell differentiation and mineralization in comparison with others. In Raw 264.7 microphages, PMMA bone cements and eluates can inhibit osteoclast formation but there was no significant difference among 4 types of bone cements. The aim of this research is to investigate the influence of PMMA bone cements on osteoblasts and osteoclasts and we hope these results can provide some useful information for the clinical application of PMMA bone cements.
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Dermal Lipogenesis Inhibits Adiponectin Production in Hs68 Fibroblasts
( Kai-chen Hu ),( Tsung-yan Tsai ),( Chu-wen Shao ),( Hsin-i Chang ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
Adiponectin is one of the most abundant adipokines from the subcutaneous fat, and regulates multiple activities through endocrine, paracrine, or autocrine mechanisms. However, its expression in adipogenic induced fibroblasts, and the potential role in photoaging has not been determined. Here, human dermal fibroblasts, Hs68, were presented as a cell model of dermal lipogenesis through stimulation of adipogenic differentiation medium (ADM). Similar to other studies in murine pre-adipocyte models (i.e., 3T3-L1), Hs68 fibroblasts showed a tendency to lipogenesis based on lipid accumulation, triglyceride formation, and the expressions of PPAR-γ, lipoprotein lipase (LPL), and FABP4 mRNA. As expected, ADM-treated fibroblasts displayed a reduction on adiponectin expression. Overall, these results suggest that dermal lipogenesis may inhibit the expression of adiponectin.
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Investigate the Mechanical and Biological Activities of PVA Membranes
( Chih-chang Yeh ),( Kai-chen Hu ),( Tsung-yan Tsai ),( Chu-wen Shao ),( Ruey-shyang Chen ),( Hsin-i Chang ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
Polyvinyl alcohol (PVA) is a water-soluble synthetic polymer and which has high tensile strength and flexibility, low toxicity and good biocompatibility for the application of soft tissue engineering. Generally, PVA can be crosslinked to form 3-D structure by a variety of methods, such as chemical crosslinking, irradiation or the freeze-thaw techniques. In contrast, chemical-crosslinked PVA structure has good elasticity, high water content, and stability at room temperature but most of crosslinking reagents such as glutaradehyde have high cytotoxicity. In here, we used low toxic genipin as crosslinking reagent for the formulation of PVA membrane. Thermal behavior and mechanical properties of genipin-crosslinked PVA membrane have been measured by thermo gravimetric analyzer and universal testing machine. Genipin-crosslinked PVA membrane had a smooth surface and the contact angel was about 67°. The swelling test displayed that genipin-crosslinked PVA membrane had high water content (130%) and the increase of PVA molecular weight can slightly improve water content. Moreover, dried genipin-crosslinked PVA membrane presented higher tensile strength (7.21MPa) but lower ductility (3.4%) than hydrated membrane (3.9MPa and 302.4%). Interestingly, rehydration of dried PVA membrane can maintain similar ductility to original hydrated membrane but the tensile strength was reduced by 40%. Therefore, drying process may break some hydrogen bonding between the polymer structure of PVA membrane. In MTT assay, there was some toxicity remained in genipin-PVA membrane but cell survivability was 1.5-1.9 fold higher than glutaradehyde-crosslinked PVA membrane. Overall, genipin-crosslinked PVA membrane showed low toxicity, good cell attachment and growth of human foreskin fibroblasts. After the cell culture study, the degradation of genipin-crosslinked PVA membrane was investigated by scanning electron microscope. Therefore, genipin-crosslinked PVA membrane is a biodegradable material and which can apply in soft tissue engineering due to its good elastic behavior.
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