骨隨細胞에서의 染色體 分折法에 대한 硏究

김태전 서울保健大學 1987 論文集 Vol.7 No.1

초파리 자연집단의 유해유전자 빈도의 변화에 관한 연구

김승곤,추종길,김태전 서울保健大學 1987 論文集 Vol.7 No.1

리그닌을 분해하는 Streptomyces lavendulas SA-14에 의한 염료의 탈색

김태전,윤경하 순천향대학교 기초과학연구소 1998 순천향자연과학연구 논문집 Vol.4 No.1

This study was undertaken to investigate the activity of ligninolytic enzymes and the decolorization capability of some dyes with streptomyces lavendulas SA-14 isolated from soil and identified. Most ligninolytic enzymes exhibited the maximal activity when the strain was grown in basal medium for 6 days. Activities of LiP 2 and VAO among these enzymes exhibited 14.25 and 11.23n㏖/㎎ protein respectively. Decolorization rate of amaranth, orange Ⅱ, tropaeolin O and poly R-478 was more than 72% on 10 days incubation while that of crystal violet and malachite green was 6.7% and 6.6% respectively.

갑상선에서 세침흡입생검에 의한 세포학적 진단분석

김태전 대한임상병리사협회 1994 대한임상병리사회지 Vol.26 No.1

The Fine Needle Aspiration Biopsy Cytologic(FNABC) preparation made from 1,640 consecutive cases among 1,822 consecutive cases attending at Dept. of Histopathology, college of medicine, H university who were suspected of thryoid diseases were studied. The data analysed in aspects of sex, age, thyroid diseases and cytologic special features were showed in each thyroid disease The results were as follows. 1) The signification of fine needle aspiration biopsy cytologic method was 90%, in sex female were more than male as 93.4%, in ages the prevalence of thyroid diseases were higher in the group of 21-40 years of age(74.6%) and their mean ages was 37.6 years of age. 2) The fine needle aspiration biopsy cytologic findings of 1,822 consecutive cases showed benign, hyperplastic conditions in 1,137 cases (62.4%), inflammatorychanges in 237 cases(l5.0%), neoplasia nodules in 230 cases(12.6%), and incomplete diagnosis due to insufficient materials in 182 cases. mean ages were 35years, 33years and 45years of age in each thyroid disease. 3) 1,137 cases with benign, hyperplastic conditions were classified by 555 cases with nodular nontoxicby 455 cases with cystic changes( 40.0%), and by 127 cases (11.2 %) with hyperplastic conditions(Grave's disease)and mean ages of it`s peak incidence were 37years. 33 years and 35years of age. 4) The analysis of 273 cases with inflammatory changes showed 182 cases with chronic thyroiditis(66.7%), 73 case with subacutic thyroiditis(26.7%), and18 cases with acutic thyroiditis(6.6%). In each inflammatory disease, mean ages of it's peak incidence were 31 years, 42 years and 28 years of age. 5) The analysis of 230 cases showed 109 cases with follicular neoplasia (47.4%), papillary carcinoma(40.0%), 15 cases with medullary carcinoma(6.5%), 9 cases with Hurthle cell tumor(3.9%), 4 cases with undifferented carcinoma(1.8%), 1case with other carcinoma(0.4%) and mean ages of it`speak incidence were 35years, 36years, 44 years, 42years, 55years and 58years of age. 6) Cytologic special features of each thyroid disease were described in detail in the part of discussion and from Fig 1 to Fig 12. In the summary of above results, I thought that the fine needle aspiration biopsy cytologic method was not only usefull for the diagnosis and classification of thyroid diseases, but also recommendable for diagnosis and classification of other diseases.

Streptomyes strain에 의한 Polymeric 염료와 Azo염료들의 탈색에 관한 연구

김태전,윤경하,최한영 서울보건대학 1998 서울보건대학 부설 한국보건과학연구소 논문집 Vol.5 No.1

Two Actinomycetes strains that it was excellent for degradablitiy of lignocellulose and lignin, and the highest for decolorization ratio of Remazol brilliant blue R(RBBR) dye, which was good relationship with degradabiliy of lignocellulose and lignin were identified finally with Streptomyces halstedii scabies SAl-36과 Streptomyces lavendulas SA2-14. Authors used these two strains to examine into the visiable spectrum and decolorization ratios of Azo dyes : amaranth, metanil yellow, orangeII. tropaeolin O, congo red, and Polymeric dye; poly R-478. The results to be examined were summarized as following. ① The visible spectrum of poly R-478, and amaranth, congo red. metanil yellow, orange II, tropaelin O were indicated that poly R-478 was 520/350nm. and amaranth. 522/324nm ; congo red. 490/434nm ; metanil yellow. 436/380nm ; orange II 484/310nm ; Tropaeolin O, 410/324 nm ② Each decolorization ratios of Azo dyes and Polymeric dye by Streptomyces halstedii scabies SA1-36 were showed that tropaeolin O was 79.3% ; poly R-478, 78.8% ; amaranth, 75.4%; orange II, 67.0% ; metanil yellow, 51.4% ; and congo red, 47.5%, so that the decolorization ratios of tropaelin O was the highest, and congo red, lowest. Consequently Streptomyces halstedii scabies SA1-36 was regarded as a good strain for the decolorization of tropaelin O into Azo dyes and poly R-478. ③ Each decolorization ratios of Azo dyes and Polymeric dye by Streptomyces lavendulas SA2-14 were showed that tropaeolin O was 81.4%; poly R-478, 80.0%; orangeII, 74.1%; congo red, 65.0%; metanil yellow, 57.7%, so that the decolorization ratios of tropaelin O was the highest. and secondly poly R-478. higher. and other dyes indicated the decolorization ratios of 57.7%-74.1%. Consequently Streptomyces halstedii scabies SA1-36 was regarded as a good strain than Streptomyces halstedii scabies SA1-36, and a good strain for the decolorization of tropaelin O into Azo dyes and poly R-478. ④ Each decolorization ratios of Azo dyes and Polymeric dye by Streptomyces badius ATCC 39117 (control group) were showed that tropaeolin O was 80.3% ; poly R-478, 79.0% ; amaranth, 70.7%; orange II, 66.4% ; congo red, 57.4% ; methenil yellow, 50.3%, so that the decolorization ratios by control group was the highest in tropaelin O, and secondly higher in poly R-478 as well as two experimental strains. ⑤ Two experimental strains were decolorized all of the examined dyes at the early days of culture, but control group, at the latter days of culture. In the above results, two experimental strains that were identified as Streptomyces halstedii scabies SAl-36과 Streptomyces lavendulas SA2-14 was regarded as a good strains for the decolorization of tropaelin O into Azo dyes and Poly R-478, and indicated 1-8% of high decolorization ratios than control group in all of Azo dyes and poly R-478, and among three strains Streptomyces lavendulas SA2-14 was thought as the best strain, when it were based on the days of culture, two experimental strains were decolorized all of the examined dyes at the early days of culture, but control group. at the latter days of culture. In the future, it will be necessary to invastigate what kind of dyes series with the object of more dyes which are decolorized more effectively by two experimental strains, also it will be more important subjects to examine into enzymes produced by Actinomycetes and establish exactly mechanism of decolorization by various produced enzymes.

한국 토양에서의 방선균 분리와 PAH화합물의 분해능에 관한 연구 : Rimazol Brillant Blue R염료의 탈색을 중심으로

김태전 서울保健大學 1995 論文集 Vol.15 No.1

Decolorization of remazol brilliant blue R(RBBR)dye, which can take the place of the degradation of polycyclic aromatic hydorcarbons(PAHS) by actinomycetes isolated in 67 soil samples collected at 8 areas of Korea, and parameters that may influence decolorization were examined from February, 1994 to March, 1995. The results were summarized as following. ① Each actinomycetes isolated on Al medium, A2 medium and A3 medium from 67 soil samples were 822 strains, 763 strains, and 783 strains. therefore Al selective medium was very good for isolation of actinomycetes ② The optimal pH of soil samples for growth of actinomycetes was 7.0∼7.5. ③ The optimal temperature for degradation of lignocellulose, lignin, and decoloization of RBBR(0.005%)dye was 30℃. ④ Strains screened in A1S medium for the degradation of lignocellulose were 205 strains among 822 strains isolated in Al medium, strains screened in M3 IND medium for degradation of lignin were 108 strains among 205 strains, and 108 strains were identified finally with one strain of amycolata autotrophica and 13 strains of streptomyces. Strains indicated decolorization ratios over 50% by agar plug and standing culture were 17 strains among 108 strains, and strains showed decolorization ratios over 60% by cell suspension (O.D; 0.85, inoculation volume: 0.2ml) and shaking culture were 12 strains among 17 strains. ⑤ Decolorization ratios of 12 strains according to carbon sources(0.2%) were very good in glycerin with 73.2% to streptomyces halstedii scabies SAl-36 and in cellobiose with 73.1% to streptomyces lavendulas SA2-14. ⑥ Decolorization ratios according to the concentrations of cellobiose to streptomyces lavendulas SA2-14 and of glycerin to streptomyces halstedii scabies SAl-36 were the most high ratios with 74.1% in 0.25% cellobiose and with 73.4% in 0.25% glycerin. In the above results, it will be possible in the future to screen actinomycetes which can degrade PAH compounds included in natural, industrial wastes and stain solution discharged during to stain tissue preparations through decolorization abillity of RBBR dye by actinomycetes.

血液 및 尿中 水銀濃度의 統計學的인 比較 檢討

김승곤,김태전 서울保健大學 1986 論文集 Vol.6 No.1

세포학적인 검사를 통해 자궁경부암의 조기 발견과 합리적인 병원내 산부인과의 운영 체계에 관한 고찰

김태전,배형준,김종열 서울보건대학 1996 서울보건대학 부설 병원경영연구소 논문집 Vol.2 No.1

In this study, the authers have investigated a true appreciation on the importance and the actual conditions of utilization of cytologic examination in gynecologic services with 3 step, that is, in 1 step the importance of cytologic examination in gynecologic services has been investigated from the results of 4,024 female of cytologic examination, in 2 step the actual conditions of utilization of cytologic examination have been investigated from 60 medical institutes through questionaire, and in 3 step the more detailed subjects of cytologic examination in gynecologic services have been investigated from 3 medical institutes selected randomly, such an investigations were done to make the programs of improvement in order to promote the activation of gynecologic medical services that shall be able to decrease occurrence of uterine cervical cancer, which is still the most prevalent cancer in korean female, and to make those on effective diagnosis and treatment activaton that shall be able to increase the income of medical institutes. The programs of improvement according to conclusions obtained from investigations like these were summarized as the following. 1. Program to activate positively cytologic examination It was well known that good correlation was noted between number of cytologic examination and those of histologic examination, those of gynecology procedures, and those of gynecologic surgeries. But it was a fact that a most of gynecology board certified physicians and physicians, and medical institutes have not utilized actively cytologic examination in gynecologic practice. Therefore all of married woman should be had politically cytologic examination at regular intervals in order to prevent and detect early uterine cervical cancer. 2. Program to establish exclusively responsible managed systems Managed systems that are exclusively responsible for cytologic examination should be established in order to have examination at regular intervals exactly(six months). 3. Program to utilize correctly the current medical insurance regulations about medical treatment charges The medical charges of cytologic exmination should be applied correctly to the current medical insurance regulations about medical treatment charges in order to increase the income of medical institutes. Because the medical charges of cytologic examination for the levels of medical check up and preventive medicin exception cancer or clinically suspicious cancer diagnosed by physicians is applicable to general medical treatment charges under the current medical insurance regulations. 4. Program to utilize reasonable diagnostic system in cytologic examination. Since certificated technologist screen first thing many microscopic slides for cytologic examination, lastly gynecologic board should make a final diagnosis on only abnormal slides. It is reasonable diagnostic system that can observe more microscopic slides, decrease misdiagnosis and high personnel expenses, and increase income in cytologic examination. Consequently it is necerssary to establish reasonable diagnostic system. 5. Program in order to promote the efficiency of the following examination and treatment system for patients to whom abnormal cell were detected. The following examination and treatment system that were corresponded with diagnosis, and automated and standardized system from receipt to gynecologic practice should be established to offer patients ease and trust, and also medical institutes the more income through the activation of gynecologic practice. Finally, the authers think that it is very important that cytologic examination should be included in routine gynecologic practice.

한국산 족부 백선균의 항진균제 감수성에 관한 연구

김승곤,김태전,이태수 서울保健大學 1997 論文集 Vol.17 No.1

Minimum inhibitory concentration(MIC) of griseofulvin, imidazole derivatives such as ketoconazole, econazole, miconazole and itraconazole on 52 strains of Trichophyton ruhrum, 22 strains of T. mentagraphytes, 10 strains of Microsporum spp. and 1 strain of Epidermophyton floccosum were determined after one week of incubation at 25C on Sabouraud dextrose broth. The results were summarized as followes : 1. The MICs of griseofulvin against 4 different tinea pedis fungi were ranged from 10-100mcg/ml. This values indicated that effectiveness of antifungal activity were relatively lower than those of three other imidazole derivatives such as ketoconazole, econazole, miconazole and one itraconazole. 2. Out of three imidazole derivatives, econazole had relatively higher inhibitory concentrations with 0.1-10mcg/ml against all tinea pedis isolated. 3. Out of 5 antifungal agents, itraconazole which is trizole derivatives showed the highest inhibitory activity with 0.1-10mcg/ml against all tinea pedis tested. 4. These results indicated that inhibitory activity of griseofulvin against tinea pedis were lower than those of four other antifungal agents tested. But these four antifungal agents such as ketoconazole, econazole, miconazole and. itraconazole were effective against tinea pedis tested.

토양에서 분리된 방선균들에 의한 Rimazol Brilliant Blue R(RBBR) 염료의 탈색에 질소원과 pH가 미치는 영향

김태전,김승곤,윤경하 서울保健大學 1997 論文集 Vol.17 No.1

Many workers had reported that the degradation of lignocellulose and lignin by white rot fungi were significantily correlative with the degradation of phenol or PAHs (Polycyclic aromatic hydorcarbons) compounds and the decolorization of a various dyes, specially RBBR (Remazol brilliant blue R) and Poly R-478 dyes. but it were not known distinctly that the degradation of these and the decolorization of these by Actinomycetes were related to the degradation of phenol or PAHs compounds, and author had examined in relationship between the degradation of lignocellulose or lignin and the decolorization of RBBR by Actinomycetes isolated in 67 soil samples collected at 8 areas of Korea from February, 1994 to March, 1995. In the experiment, There were selected two strains identified as Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 which had good correlation between the degradation of lignocellulose or lignin, and it were investigated that the cultural parameters to maximize the decolorization ratios of RBBR by two strains, as the results, Streptomyces halstedii scabies SA1-36 revealed each the highest decolorization ratios with 73.4% in 0.30% glycerin and Streptomyces lavendulas SA2-14, with 74.1% in 0.25% cellobiose at 30℃ in 20 days of culture. There were used their results as basal data in this study that examined the effects of nitrogen sources and pH to maximize the decolorization ratios of RBBR. It were summarized as following that the results examined the effects of nitrogen sources and pH to maximize the decolorization ratios of RBBR by two strains. 1) It were the highest each as 75.7% in casein and 75.7% in yeast extract that the dicolorization ratios of RBBR by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains according to nitrogen sources(0.l%). 2) It were the highest each as 76.8% in 0.1% casein and 78.3% in yeast extract that the decolorization ratios of RBBR by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains according to the concentrations of casein and yeast extract. 3) It were the highest each as 76.8% in pH 7.6 and 79.4% in pH 6.6 that the decolorization ratios of RBBR according to pH by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains. In the above results, we could knowe that it were each different as well as carbon sources that nitrogen sources and pH required between Streptomyces halstedii scabies SAl-36 and Streptomyces lavendulas SA2- 14 strains. and organic nitrogens influence more than inorganic nitrogens to decolorize RBBR dye and nitrogen sources and pH influence lower than carbon sources to decolorize RBBR dye. What`s more, we could knowe that it were very important to use newly formuliged optimal basal media maximize decolorization ratios of RBBR dye by Streptomyces halstedii scabies SAl-36 and Streptomyces lavendulas SA2-14 strains.