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RISS 인기검색어
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- 저자 : ( Taeh Yeon Jeon ) (검색결과 1 건)
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( Sun Jung Kwon ),( Taeh Yeon Jeon ),( Dong Wook Seo ),( Moon Joon Na ),( Eu Gene Choi,),( Ji Woong Son ),( Eun Hyung Yoo ),( Chang Gyo Park ),( Hoi Young Lee ),( Ju Ock Kim ),( Sun Young Kim ),( Jae 대한결핵 및 호흡기학회 2012 Tuberculosis and Respiratory Diseases Vol.72 No.3
Background: Ventilator-associated pneumonia (VAP) requires prompt and appropriate treatment. Since methicillin- resistant Staphylococcus aureus (MRSA) is a frequent pathogen in VAP, rapid identification of it, is pivotal. Our aim was to evaluate the utility of quantitative polymerase chain reaction (qPCR) as a useful method for etiologic diagnoses of MRSA pneumonia. Methods: We performed qPCR for mecA, S. aureus-specific femA-SA, and S. epidermidis-specific femA-SE genes from bronchoalveolar lavage or bronchial washing samples obtained from clinically-suspected VAP. Molecular identification of MRSA was based on the presence of the mecA and femA-SA gene, with the absence of the femA-SE gene. To compensate for the experimental and clinical conditions, we spiked an internal control in the course of DNA extraction. We estimated number of colony-forming units per mL (CFU/mL) of MRSA samples through a standard curve of a serially-diluted reference MRSA strain. We compared the threshold cycle (Ct) value with the microbiologic results of MRSA. Results: We obtained the mecA gene standard curve, which showed the detection limit of the mecA gene to be 100 fg, which corresponds to a copy number of 30. We chose cut-off Ct values of 27.94 (equivalent to 1×104 CFU/mL) and 21.78 (equivalent to 1×105 CFU/mL). The sensitivity and specificity of our assay were 88.9% and 88.9% respectively, when compared with quantitative cultures. Conclusion: Our results were valuable for diagnosing and identifying pathogens involved in VAP. We believe our modified qPCR is an appropriate tool for the rapid diagnosis of clinical pathogens regarding patients in the intensive care unit.
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