사람 치은 섬유아세포에서의 Tannerella forsythia 전세균, 막단백질, 당지질에 의한 염증성 사이토카인 발현

김정은,이성훈,최봉규,구기태,김태일,이용무,구영,정종평,류인철,Kim, Jung-Eun,Lee, Sung-Hoon,Choi, Bong-Kyu,Koo, Ki-Tae,Kim, Tae-Il,Lee, Yong-Moo,Ku, Young,Chung, Chong-Pyoung,Rhyu, In-Chul 대한치주과학회 2008 Journal of Periodontal & Implant Science Vol.38 No.3

Purpose: The purpose of this study was to investigate induction of cytokine expression in human gingival fibroblasts (HGFs) by whole cell and the components of T. forsythia. Material and Methods: After HGFs were treated with lipopolysaccharide (LPS), membrane protein isolated from T. forsythia or culture media of T. forsythia, the induction of interleukin (IL)-1, IL-6 and IL-8 was examined with real-time PCR and ELISA. Their induction ability of cytokines was compared with whole bacteria. Result: The expression of IL-6 and IL-8 was significantly induced in HGFs by whole bacteria and membrane protein. The expression of IL-$1{\beta}$ was induced by membrane protein of T. forsythia, not by whole bacteria. LPS and condition media of T. forsythia slightly activated HGFs. Conclusion: The membrane protein of T. forsythia could be one of virulence factors.

치과의원과 대학치과병원 교정환자의 통제소재 특성

구승준,이신재,김태우,장영일 대한치과교정학회 2006 대한치과교정학회지 Vol.36 No.6

환자가 의료기관을 선택할 때 작용하는 요인은 매우 다양하며 이에 대한 많은 연구들이 있었으나 대부분 외부적인 요인에 대한 연구가 많았고 의료기관 선택 시 환자의 정신-심리학적인 면에 대한 정보는 부족한 실정이다. 이에 본 연구에서는 비교적 선택진료(elective treatment)의 측면이 많은 교정치료를 받기 위해 치과의원과 대학치과병원에 내원한 환자들이 의료기관을 선택하는 여러 요인들 중 정신-심리학적 요인을 알아보기 위해 인간의 행동을 예측하는데 유용하다고 알려진 통제소재(Locus of control)검사를 이용하였다. 교정치료를 목적으로 서울대학교 치과병원에 내원한 환자 934명 및 치과의원에 내원한 환자 1466명을 대상으로 내외통제소재검사를 시행한 결과 대학치과병원 교정환자들이 치과의원 교정환자에 비하여 외적통제 경향이 높았으며 이러한 결과에 비추어 대학치과병원 교정환자들이 치과의원 교정환자들에 비해 의료진의 권위에 의존하는 정신-심리학적 경향이 존재하는 것으로 나타났다. Objective: Mary factors are considered when a patient chooses the type of dental institution they wish to receive treatment from. Numerous studies have been conducted regarding this issue. However, more research is needed in analyzing the patient's psychological aspects when he/she decides to choose the dental institution they wish to receive treatment from. Methods: A locus of control test was utilized to analyze the psychologica1 aspects in orthodontic patients of a university denta1 hospital and a private dental clinic. The locus of control test is known to be useful in predicting a human being's behavior Both intrinsic and extrinsic locus of control tests were carried out in 934 orthodontic patients attending Seoul National University Dental Hospital and 1466 orthodontic patients from a private dental clinic. Results: The orthodontic patients at the university dental hospital showed a stronger extrinsic locus of control compared with those from the private dental clinic. Conclusion: The orthodontic patients at the university dental hospital value the doctor as the authority figure and the treatment quality more than those at the private dental clinic.

한국판 임상가용 아동 및 청소년을 위한 외상후 스트레스장애 척도의 신뢰도 및 타당도 연구

구정일,김태형,은헌정,최말례,이선미,조수진,송옥선,김형욱 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.6

Objectives : This present study was conducted to explore the reliability and the validity of Clinician Administered PTSD Scale for Children and Adolescents Korean version (CAPS-CA) Mcthods : The Subjects were children and adolescents (8-13yrs) from different places: a certain elementary school, three orphanages, a general hospital, and two neuropsychiathc local clinics. Only subjects who experienced a catastrophic traumatic event were tested by CAPS-CA, CDI, RCMAS, and TSC. Rcsults : The range of correlations between item-criterion were .67-.84. The coefficients of internal consistency were .87. and test-retest reliability was .90, were all the coefficients in the proper range. Inter-correlations of CAPS-CA, CDI, RCMAS, and TSC scale showed significant correlation. TSC showed the highest correlation coefficient. CAPS-CA can be a useful clinician-administered diagnostic instrument for children and adolescents PTSD symptoms. Conclusion : The reliability and the validity of CAPS-CA were confirmed. CAPS-CA could be applied for diagnostic purposes of PTSD in children and adolescents.

사무직 근로자들의 직무 스트레스와 피로

차경태,김일희,고상백,현숙정,박준호,박종구,차봉석,장세진 大韓産業醫學會 2008 대한직업환경의학회지 Vol.20 No.3

목적: 이 연구는 전국 규모의 조사 연구에서 수집된 자료 중 사무직 근로자들을 대상으로,사회인구학적 특성,직업 특성,건강행태,직무 스트레스와 피로수준 간의 관련성을 분석하는 데 있다. 방법:‘한국인 직무스트레스 측정도구의 표준화 전국 조사연구National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002∼2004)’를 위해 2002년 5월 1일부터 2003년 5윌 30일 까지 수행되었으며,전국의 사업장에 근무하는 근로자 30,146명(남자: 84%,여자: 16%) 중 사무직 근로자 4,457명(남자: 68.9%,여자 31.1%)을 연구 대상으로 하였다. 자료수집방법은 사업장을 방문하여 보건 및 안전관리자 등에게 연구의 취지를 알린 후 설문조사에 대한 협조를 구하고 응답자 직접 기입법을 이용 하였으며,사회인구학적 특성,직업적 특성,건강관련 요인,KOSS-SF를 이용한 7개의 직무 스트레스 요인,그리고 MFS를 이용하여 피로 수준에 영향을 주는 요인에 대해 분석하였다. 결과: 일반적 특성,작업관련 특성,건강행태관련 특성 및 직무 스트레스 요인이 근로자들의 피로와 강한 관련성이 있음이 입증되었으며,특히 직무 스트레스의 하부 요인 중에서도 피로와 관련성을 보이는 요인이 남녀별로 다소 상이 하게 관련된다는 것을 발견할 수 있었다. 남자의 경우는 직무 스트레스의 하부 영역 중 직무 요구도,직무 불안정성,보상부적절 등이 영향력이 높은 주요 직무 스트레스 요인이었던 반면,여자는 직무 요구도,보상부적절,직장문화가 주요한 직무 스트레스 요인이었다. 결론: 직장인 피로가 갖는 보건학적 의학적 경제학적 중요성을 감안할 때,조직의 생산성 향상과 근로자 개개인의 삶의 질 향상 및 건강증진을 위해선 직무 스트레스 관리 프로그램이 절실히 요청된다고 볼 수 있다. Background & Objectives: A growing body of research has documented that occupational stress is closely associated with increased risk of fatigue. This study was conducted in an effort to assess the relationship of occupational stress to self-perceived fatigue among Korean white collar employees. Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project, 2002-2004). Among them, a total of 4,502 white collar employees were recruited. A structured questionnaire was employed to evaluate the participants' sociodemographics, job-related factors, health-related behaviors, occupational stress, and self-perceived fatigue. Occupational stress and self-perceived fatigue were assessed using the Korean Occupational Stress ScaleShort form (KOSS-SF) and the Multidimensional Fatigue Scale (MFS), respectively. Results: In logistic regression analyses, occupational stress was associated with an increased risk of fatigue, and some domains of occupational stress had different effects on fatigue by gender (job demand, job insecurity, and lack of reward for men, job demand, lack of reward, and discomfort in occupational climate for women), which indicates that occupational stress may perform a slightly different role in increasing the risk of fatigue by gender. Conclusion: The results of this study suggest that occupational stress is a determinant predictor of self-perceived fatigue. Thus, a stress management program for the reduction of occupational stress, and the promotion of white collar worker's health and quality of life is strongly recommended. Some limitations of this study and considerations for future studies were also discussed.

Amelioration of inflammatory responses by <i>Socheongryong-Tang</i> , a traditional herbal medicine, in RAW 264.7 cells and rats

Park, Sang Mi,Lee, Tae Hoon,Zhao, Rongjie,Kim, Youn Sook,Jung, Ji Yun,Park, Chung A.,Jegal, Kyung Hwan,Ku, Sae Kwang,Kim, Jae Kwang,Lee, Chul Won,Kim, Young Woo,Cho, Il Je,An, Won G.,Kim, Sang Chan D.A. Spandidos 2018 International journal of molecular medicine Vol.41 No.5

<P>Socheongryong-Tang (SCRT) is a natural medicine prescription that has been mainly used in East Asia for the treatment of inflammatory disorders, including asthma and allergic rhinitis. The present study evaluated the anti-inflammatory effects of SCRT on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and in a rat model of carrageenan (CA)-induced paw edema. Levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 and prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) in the culture supernatant were quantified and nitric oxide (NO) production was monitored. In addition, the effect of SCRT on the protein expression of nuclear factor-κB (NF-κB), mitogen-activated protein kinases (MAPKs), inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) was assessed by western blot analysis. Furthermore, the effects of SCRT on acute inflammation <I>in vivo</I> and changes in the histomorphometry and histopathology of paw skin were observed using CA-treated rats. SCRT (1 mg/ml) inhibited the LPS-induced changes in the protein expression of NF-κB, JNK, ERK1/2, iNOS and COX-2, as well as the production of NO, PGE<SUB>2</SUB> and cytokines. In the rat paw edema assay, administration of 1 g/kg of lyophilized powder obtained from the aqueous extracts of SCRT for 3 consecutive days inhibited the CA-induced increases in skin thickness, mast cell degranulation, and infiltration of inflammatory cells in the ventral and dorsal pedis skin within 4 h. These results demonstrated that SCRT exerts its anti-inflammatory activities in LPS-stimulated RAW 264.7 cells through decreasing the production of inflammatory mediators, including PGE<SUB>2</SUB>, NO and cytokines, via suppression of the NF-κB and JNK and ERK1/2 signaling pathways. In addition, the data of the CA-induced paw edema indicated an anti-edema effect of SCRT. SCRT (1 g/kg) reduced acute edematous inflammation through inhibition of mast cell degranulation and infiltration of inflammatory cells. Therefore, the present study provided scientific evidence for the anti-inflammatory activities of SCRT as well as the underlying mechanisms.</P>

Inhibitory Effects of Traditional Herbal Formula Pyungwi-San on Inflammatory Response <i>In Vitro</i> and <i>In Vivo</i>

Cha, Ji Young,Jung, Ji Yun,Jung, Jae Yup,Lee, Jong Rok,Cho, Il Je,Ku, Sae Kwang,Byun, Sung Hui,Ahn, Yong-Tae,Lee, Chul Won,Kim, Sang Chan,An, Won G. Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>Pyungwi-san (PWS) is a traditional basic herbal formula. We investigated the effects of PWS on induction of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), pro-inflammatory cytokines (interleukin-6 (IL-6) and tumor necrosis factor-<I><I>α</I></I> (TNF-<I><I>α</I></I>)) and nuclear factor-kappa B (NF-<I><I>κ</I></I>B) as well as mitogen-activated protein kinases (MAPKs) in lipopolysaccharide-(LPS-) induced Raw 264.7 cells and on paw edema in rats. Treatment with PWS (0.5, 0.75, and 1 mg/mL) resulted in inhibited levels of expression of LPS-induced COX-2, iNOS, NF-<I><I>κ</I></I>B, and MAPKs as well as production of prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>), nitric oxide (NO), IL-6, and TNF-<I><I>α</I></I> induced by LPS. Our results demonstrate that PWS possesses anti-inflammatory activities via decreasing production of pro-inflammatory mediators through suppression of the signaling pathways of NF-<I><I>κ</I></I>B and MAPKs in LPS-induced macrophage cells. More importantly, results of the carrageenan-(CA-) induced paw edema demonstrate an anti-edema effect of PWS. In addition, it is considered that PWS also inhibits the acute edematous inflammations through suppression of mast cell degranulations and inflammatory mediators, including COX-2, iNOS and TNF-<I><I>α</I></I>. Thus, our findings may provide scientific evidence to explain the anti-inflammatory properties of PWS <I>in vitro</I> and <I>in vivo</I>.</P>

Maternal and Fetal Risk Factors Associated with Neonatal Intensive Care Unit Admission in Term Neonates

( Tae Il Ku ),( Young Nam Kim ),( Do Hwa Im ),( Ye Seul Kim ),( Da Hyun Kim ),( Jung Mi Byun ),( Dae Hoon Jeong ) 대한주산의학회 2021 Perinatology Vol.32 No.4

Objective: This study aimed to evaluate the risk factors associated with neonatal intensive care unit (NICU) admission in term neonates and to provide necessary data for antenatal counseling. Methods: This retrospective case-control study was conducted with 643 singleton pregnancies with term delivery at Inje University Busan Paik Hospital from 2017 to 2019. Patients were divided into the NICU admission and nursery groups. Maternal, antepartum, intrapartum, and placental factors were compared between the 2 groups to evaluate the risk factors for the NICU admission. Neonatal outcomes were analyzed as well. Results: NICU-admitted neonates had lower birth weight (2,971±597 vs. 3,142±418 g, P<0.001), higher incidence of fetal growth restriction (FGR) (21.6% vs. 0.2%, P<0.001), lower Apgar score at 5 minutes (2.7% vs. 0%, P=0.001), and higher incidence of respiratory distress syndrome (23.9%, n=53). Fetal growth restriction (FGR) was the greatest risk factor for the NICU admission (odds ratio [OR], 126.98; 95% confidence interval [CI], 17.17-939.17). Maternal pre-gestational and gestational diabetes mellitus with insulin use were great risk factors for the NICU admission (OR, 33.74; 95% CI, 4.36-260.92 for pre-gestational diabetes; and OR, 24.61; 95% CI, 3.02-200.07 for gestational diabetes, respectively). Emergent cesarean delivery due to prolonged 1st stage of labor and vaginal delivery had similar risk for the NICU admission (OR, 2.18; 95% CI, 1.26-3.77; and OR, 1.85; 95% CI, 1.18-2.91, respectively). Conclusion: Pregnancies that are complicated with FGR, pre-gestational and gestational diabetes mellitus with insulin use, and emergent cesarean delivery due to prolonged 1st stage labor may have higher risk for NICU admission, even among term neonates. Therefore, pregnancies with the above risk factors are advised for possible NICU admission during antenatal counseling.

Comparison of strains of pelvic inflammatory disease and tubo-ovarian abscess

( Tae Il Ku ),( Eun Hye Cho ),( Do Hwa Im ),( Da Hyun Kim ),( Jung Mi Byun ),( Young Nam Kim ),( Kyung Bok Lee ),( Moon Su Sung ),( Dae Hoon Jeong ) 대한산부인과학회 2022 대한산부인과학회 학술대회 Vol.108 No.-

Objective: This study aimed to evaluate the comparison of strains of pelvic inflammatory disease(PID) and tubo-ovarian abscess(TOA) to provide necessary data for treatment and counseling of the inflammatory diseases of the female genital tract. Methods: This retrospective case-control study was conducted with 264 patients with confirmed PID and 153 patients with confirmed TOA at Inje University Busan Paik Hospital from 2010 to 2019. Endocervical swabs were aseptically collected from Pelvic infammatory patients and TOA patients for comparing between the each groups to evaluate the strains for the diseases. Results: Sexual transmitted organisms encountered in PID were Ureaplasma urealyticum(49.2%, n=130), Chlamydia trachomatis(36.4%, n=96), Mycoplasma hominis(25.0%, n=66), Neisseria gonorrhea(6.4%, n=17), Mycoplasma genitalium(1.9%,n=5), and Trichomonas(1.1%, n=3). And also Gram stain organisms encountered in pelvic inflammatory diseases were Gram(+) cocci(9%, n=42), Gram(-) rod(15.5%, n=41), Escherichia coli(11.4%, n=30), Candida albicans(6.4%, n=17), Extended-spectrum-lactamase(3.8%, n=10) and Gram(+) rod(2.3%, n=6). In TOA, Sexual transmitted organisms encountered were Ureaplasma urealyticum(35.3%, n=54), Chlamydia trachomatis(16.3%, n=25), Mycoplasma hominis(23.5%, n=36), Mycoplasma genitalium(2.6%, n=4), Neisseria gonorrhea(1.3%, n=2), and Trichomonas(0.7%, n=1). And also Gram stain organisms encountered were Gram(+) cocci(20.3%, n=31), Gram(-) rod(19.0%, n=29), Escherichia coli(13.7%, n=21), Gram(+) rod(12.4%, n=19), Candida albicans(5.2%, n=8), Extended-spectrum-lactamase(2.6%, n=4). Conclusion: According to our study, TOA had a higher proportion of non-sexual transmitted infection strains than PID. Based on this, in PID and TOA, it would be effective to use antibiotics that can cover non-sexual transmitted infection, including gram(+) cocci and gram(-) rod. In TOA in particular, it is considered to be essential to use antibiotics that can cover non-std infection.

The Effects of Transplanting Time and Meteorological Change to Variation of Phyllochron of Rice

Ku, Bon-Il,Choi, Min-Kyu,Kang, Shin-Ku,Lee, Kyung-Bo,Park, Hong-Kyu,Park, Tae-Seon,Ko, Jae-Kwon,Lee, Byun-Woo The Korean Society of Crop Science 2010 Korean journal of crop science Vol.55 No.3

This study was performed at Rice and Winter Cereal Crops Department of NICS during 2007 and 2008 to investigate the characteristics of rice leaf emergence and to obtain basic data which can be used for rice growth simulation model by which we can forecast rice growth stage and heading date accurately under different cultivars, transplanting date, and climatic conditions. To confirm leaf emergence rate according to rice maturing ecotype, we surveyed the leaf emergence rate and heading date of Unkwangbyeo, Hwayoungbyeo and Nampyeongbyeo which are early maturing, medium maturing and medium-late maturing cultivars, respectively, according to seedling raising duration and transplanting time. When seedling duration was 15 days, the growth duration between transplanting time and completion of flag leaf emergence on main culm were 51.5~78.3 days in Unkwangbyeo, 55.3~87.9 days in Hwayoungbyeo and 58.4~98.4 days in Nampyeongbyeo, respectively. When seedling duration was 30 days, they were 50.1~75.5 days in Unkwangbyeo, 52.4~84.7 days in Hwayoungbyeo and 56.4~93.8 days in Nampyeongbyeo, respectively. As transplanting time delayed, the emerged leaf number after transplanting decreased in all rice cultivars. The cumulative temperature between transplanting time to completion of flag leaf elongation on main culm were $1,281^{\circ}C{\sim}1,650^{\circ}C$ in Unkwangbyeo, $1,344^{\circ}C{\sim}1,891^{\circ}C$ in Hwayoungbyeo and $1,454^{\circ}C{\sim}2,173^{\circ}C$ in Nampyeongbyeo, respectively. Leaf emergence rate on main culm were precisely represented by equation, y = $y_0$ + a / [1 + exp( - (x - $x_0$) / b)]^c, when we used daily mean temperature as variable.

The Effects of Transplanting Time and Meteorological Change to Variation of Phyllochron of Rice

Bon-Il Ku,Min-Kyu Choi,Shin-Ku Kang,Kyung-Bo Lee,Hong-Kyu Park,Tae-Seon Park,Jae-Kwon Ko,Byun-woo Lee 韓國作物學會 2010 한국작물학회지 Vol.55 No.3

This study was performed at Rice and Winter Cereal Crops Department of NICS during 2007 and 2008 to investigate the characteristics of rice leaf emergence and to obtain basic data which can be used for rice growth simulation model by which we can forecast rice growth stage and heading date accurately under different cultivars, transplanting date, and climatic conditions. To confirm leaf emergence rate according to rice maturing ecotype, we surveyed the leaf emergence rate and heading date of Unkwangbyeo, Hwayoungbyeo and Nampyeongbyeo which are early maturing, medium maturing and medium-late maturing cultivars, respectively, according to seedling raising duration and transplanting time. When seedling duration was 15 days, the growth duration between transplanting time and completion of flag leaf emergence on main culm were 51.5~78.3 days in Unkwangbyeo, 55.3~87.9 days in Hwayoungbyeo and 58.4~98.4 days in Nampyeongbyeo, respectively. When seedling duration was 30 days, they were 50.1~75.5 days in Unkwangbyeo, 52.4~84.7 days in Hwayoungbyeo and 56.4~93.8 days in Nampyeongbyeo, respectively. As transplanting time delayed, the emerged leaf number after transplanting decreased in all rice cultivars. The cumulative temperature between transplanting time to completion of flag leaf elongation on main culm were 1,281~circC~1,650~circC in Unkwangbyeo, 1,344~circC~1,891~circC in Hwayoungbyeo and 1,454~circC~2,173~circC in Nampyeongbyeo, respectively. Leaf emergence rate on main culm were precisely represented by equation, y = y0 + a / [1 + exp( - (x - x0 ) / b)]c, when we used daily mean temperature as variable.