Korean Red ginseng prevents endothelial senescence by downregulating the HO-1/NF-κB/miRNA-155-5p/eNOS pathway

Tae-Hoon Kim,Ji-Yoon Kim,Jieun Bae,Young-Mi Kim,Moo-Ho Won,Kwon-Soo Ha,Young-Guen Kwon,Young-Myeong Kim 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.2

Background: Korean Red ginseng extract (KRGE) has beneficial effects on the cardiovascular system by improving endothelial cell function. However, its pharmacological effect on endothelial cell senescence has not been clearly elucidated. Therefore, we examined the effect and molecular mechanism of KRGE on the senescence of human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were grown in normal or KRGE-supplemented medium. Furthermore, they were transfected with heme oxygenase-1 (HO-1) gene or treated with its inhibitor, a NF-κB inhibitor, and a miR-155-5p mimic or inhibitor. Senescence-associated characteristics of endothelial cells were determined by biochemical and immunohistochemical analyses. Results: Treatment of HUVECs with KRGE resulted in delayed onset and progression of senescence-associated characteristics, such as increased lysosomal acidic β-galactosidase and decreased telomerase activity, angiogenic dysfunction, and abnormal cell morphology. KRGE preserved the levels of anti-senescent factors, such as eNOS-derived NO, MnSOD, and cyclins D and A: however, it decreased the levels of senescence-promoting factors, such as ROS, activated NF-κB, endothelial cell inflammation, and p21 expression. The beneficial effects of KRGE were due to the induction of HO-1 and the inhibition of NF-κB-dependent biogenesis of miR-155-5p that led to the downregulation of eNOS. Moreover, treatment with inhibitors of HO-1, NF-κB, and miR-155-5p abolished the anti-senescence effects of KRGE. Conclusion: KRGE delayed or prevented HUVEC senescence through a signaling cascade involving the induction of HO-1, the inhibition of NF-κB-dependent miR-155-5p biogenesis, and the maintenance of the eNOS/NO axis activity, suggesting that it may protect against vascular diseases associated with endothelial senescence.

Synthesis of Sr Doped HoCrO3 Solid Solution Systems and Their Electrical Properties

PARK, SUNG HO,Kwon, Tae Yun 한국공업화학회 2000 Journal of Industrial and Engineering Chemistry Vol.6 No.4

Ho_(1-x)SrxCrO₃ systems, with perovskite structures, were synthesized for x=0, 0.05, 0.1, 0.2 at 1300 ℃ under atmospheric pressure. The results of X-ray analysis reveals that these prepared systems forms orthorhombic structures. Also, the HoCrO₃ systems which were doped with Sr cations on the site of Ho cations, exhibited a linear increase in their cell volume. It was found that the lattice parameter and reduced volume increased as the doping level of Sr^(2+) increased. From the measurements of D.C conductivity, the activation energy obtained from the conductivity dependence on temperature was 0.18 eV. The conduction mechanism would appear to be the hopping model of electron conduction.

역행적 골수정 삽입술을 이용한 대퇴골 과상부 골절의 치료

권진우,신승호,조원호,성기호,김기환,손경태,이상훈,박세원,배종문,권태우 대한골절학회 2003 대한골절학회지 Vol.16 No.4

생태건축의 실현을 위한 목조건축의 역할에 관한 고찰

권태호 世明大學校 1998 世明論叢 Vol.7 No.-

Our current efforts for the propulsion of 'Environmentally Sound and Sustainable Development(ESSD)' is seen as the result from global introspection of the excessive encroachment on the ecosystem under the name of so-called economic development through rapid industrialization as well as urbanizaion. Thus, the concept of the ESSD has emerged as one of the central issues in many disciplines including architecture in which the employement of green, reusable and/or recyclable building materials is strongly emphasied. With this in view, the present paper, paying attention to the failure of modernizing and accommodation korean traditional wood structure due to the rapid industrialization over 40 years, attempts to ealborate and justify the potentials and roles of wood materials for the accomplishment of ecological architecture in Korea. In addition, major associated reserch areas and policy directions are included inthe conclusion and suggestions.

대장선암 조직의 CEA에 관한 면역조직학적 고찰

권오준,신현호,서인수,손태중 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.4

The autors immunohistochemically studied 63 cases of colorectal adenocarcinoma to evaluate clinical significance of histologic pattern of tissue CEA. Immunohistochemical stain of cancerous tissues was performed by ABC method, and then light and electron microscopy were carried out. On light microscopic studies, CEA was mainly distributed on apical and lateral surface of normal and cancer cells and weakly positive in the cytoplasm of cancer cells. On electron microscopic studies, CEA was strongly positive along the apical and lateral borders of cancer cells and weakly positive in the cytoplasm as a pattern of fine granules. The statistical studies revealed such a data that high preoperative serum CEA level was shown in cases of tumor necrosis. Also in groups of surrounding invasion, well differentiation, and distant metastasis of the cancer, preoperative serum CEA levels were significantly increased. Thus, the evaluatin of CEA serologically and immunocytochemically in cases of colorectal adenocarcinoma may significantly inform in diagnosis or postoperative treatment and follow-up.

종이상자포장기의 개발에 관한 연구

권경우,권병철,류봉조,명태식,박찬호,이광원 한밭대학교 산업과학기술연구소 1994 논문집 Vol.2 No.-

The paper describes the development of a new automatic wrapping machine using paper films as it has not been used so far. The most of the automatic wrapping machine have used the vinyl film causing environmental pollution, the paper films, however, have a weak point in the aspect of high temperature adhesion. Therefore, the mechanism of the existing wrapping machine should be altered to prevent it from curling effect through the technical know-how. A new method and technology reducing the curling effect in the automnatic wrapping machine are demonstrated by using developed blower. Thus it is exqection and will decrease environmental pollution.

대구대학교 경산캠퍼스의 야생조류분포에 관한 연구

권태호,조창근 大邱大學校出版部 (대구대학, 한사대학 1989 農業科學硏究論文集 Vol.3 No.-

This study was executed to investigate the avifauna in Kyongsan campus of Taegu University. 40 species and 618 individuals of wildbird were observed in campus and those consis of 18 species for resident. 18 species for summer visitor, 3 species for winter visitor and 1 species for passage migrant. The living density was averagely 4.79 birds per hectare and the dominant species were Passer montanus(24.27%), Hirundo rustica(12.62%), Paradoxomis webbiana(10.68%), Parus major(7.93%), Pica pica(5.66%), Streptopelia orientalis(4.69%) in order. Number of species and individuals and species diversities were higher in survey route II(light-used area) than in survey route I(heavy-used area). Also species diversity was the highest in June for 1.2265 and the lowest in September for 0.9076. Similarity index between two areas according to difference of use intensity was 68.85% and those indices by month represented seasonal migrations of wildbirds considerably.

Allylisothiocyanate에 依한 窒酸鹽還元酵素의 不活性化

權泰英,朴聖鎬 Natural Science Research Institute JEONJU UNIVERSI 1990 전주대학교 자연과학연구소 학술논문집 Vol.3 No.1

Inhibitory effect of glucosinolate decomposites and related substances, such as allyl-NCS. allyl-SCN, allyl-CN, allylthiourea, N'-dimethylallylthiourea. 3-butenyl-SCN, 4-pentenyl-SCN, 2-phenylethyl-SCN, thiourea, sodium thiocyanate and potassium cyanide, on the activity of nitrate reductase preparation from spinach were studied at 27℃ and pH7.8. All tested compounds, except allyl-CN, inhibited nitrate reductase at below 0.1∼1.0mM level, and ineffective on nitrate and nitrite under this experimental condition. Inhibition by allyl-NCS was non-competitive and Ki value 1.20mM. Ethanol commonly detected compound in fermented foods strongly inhibited at 1.0% (v/v_o).

付審判節次에 關한 考察

權泰鎬 청주대학교 대학원 1989 우암논총 Vol.5 No.-

As to the application by the accuser who has objected to the disposition not to institure a public prosecution by the public prosecutor, the High Court shall rule or whether the dispositions are proper or not. This system has been derived from the system of compulsory indictment of West Germany. The West Germany compulsory indictment system is under purpose to secure the legal public prosecution of the case and all the crimes are to be the object thereof and that it shall be ordered to institute public prosecution. Our system however, in contrary to West Germany system, has the function restricting the discretionary indistment like Japan and public prosecution shall be deemed to have been instituted in the case. The claimant is an accuser who has been noticed for the disposition not to institute public prosecution from the public prosecutor. The case eligible for an application shall be limited to the climes under Article 123 to Article 125 of the Criminal Code. An application shall be filed to the High Court which corresponds to the Higher Prosecutors' Office to which the public prosecutor belongs through the Chief Prosecutor of a district Prosecutors' Office to which the public prosecutor who has disposed not to institute Public prosecution in writting within 10 days from the date of receiving the notice of that disposition. If the applcation is reasonable, the High Court shall commit the case to the competent district court for trial. And, if such an application is not reasonable, the High Court shall dismiss it. The case as has been committed to the competent district court for trial shall be applied its maintenance of public prosecution by the advocate who has been disignated by the Court as the person in charge for maintenance of the public prosecution not by the public prosecutor. The advocate appointed as maintenance person of the public prosecution shall exercise, ex officio, the duties of a public prosector in order to maintain public prosecution until the Judgement is finally settled. As a legislative theory of this system I propose the following. 1. to expand objects for this system 2. to effectively connect with the public prosecutors' appeal system, and 3. to revise the procedure so that may meet with the spirit of filing only the written indictment.

Stented Graft의 임상경험

권태원,김호성,김도균,성규보,조용필,김건언 대한혈관외과학회 2000 Vascular Specialist International Vol.16 No.2

Purpose: Endovascular repair is a alternative treatment with favorable short-term result, but long term results are not available compared to standard operation. The authors have reviewed experiences of Asan Medical Center on stented graft, performed from Aug. 1998 to Feb. 2000, and the results as to age, operative indication, clinical condition, anatomic location, complication, follow-up are reported. Method: Seven patients who has received stented graft between Aug. 1998 and Feb. 2000 are reviewed retrospectively. Result: Of the seven patients, 6 were male and 1 was female. The mean age was 46.4 years (17~71). Indication for stented graft were two pseudoaneurysms, two iatrogenic arterio-venous fistulas, one thoracic aorta aneurysm, one abdominal aortic aneurysm, and one iliac artery occlusion with blue toe syndrome. Three different types of stented graft devices were utilized: Dacron-covered nitinol stented graft (made by the Asan Medical Center medical device team), Vanguard II (by Boston Scientific Corp.), and PTFE with nitinol stented graft (by Tae-woong). There was no perioperative mortality, and the technical success was achieved in five cases (71%). The patency at 14-month were 1009o (5/5). Two immediate complications are hemorrhage due to iliac & femoral artery avulsion requiring emergency bypass grafting, and deployment failure of stented graft requiring removal of retained graft device. Conclusion: Stented graft may be utilized even in complicated vascular situation but will need further development of device and more experience to prevent possible catastrophic complication. Indication should be decided by team approach and vascular surgeon should be prevent or participate during the procedure.