방사선조사가 Streptococcus mutans에 미치는 영향

안기동,김규태,최용석,황의환 대한구강악안면방사선학회 2007 Imaging Science in Dentistry Vol.36 No.1

Purpose : To observe direct effect of irradiation on cariogenic Streptooccus mutans. Materials and Methods : S. mutans GS5 was exposed to irradiation with a single absorbed dose of 10, 20, 30, and 40 Gy. Viability and changes in antibiotic sensitivity, morphology, transcription of virulence factors, and protein profile of bacterium after irradiation were examined by pour plate, disc diffusion method, transmission electron microscopy, RT-PCR, and SDS-PAGE, respectively. Results : After irradiation with 10 and 20 Gy, viability of S. mutans was reduced. Further increase in irradiation dose, however, did not affect the viability of the remaining cells of S. mutans. Irradiated S. mutans was found to have become sensitive to antibiotics. In particular, the bacterium irradiated with 40 Gy increased its susceptibility to cefotaxime, penicillin, and tetracycline. Under the transmission electron microscope, number of morphologically abnormal cells was increased as the irradiation dose was increased. S. mutans irradiated with 10 Gy revealed a change in the cell wall and cell membrane. As irradiation dose was increased, a higher number of cells showed thickened cell wall and cell membrane and lysis, and appearance of ghost cells was noticeable. In RT-PCR, no difference was detected in expression of gtfB and spaP between cells with and without irradiation of 40 Gy. In SDS-PAGE, proteins with higher molecular masses were gradually diminished as irradiation dose was increased. Conclusion : These results suggest that irradiation affects the cell integrity of S. mutans, as observed by SDS-PAGE, and as manifested by the change in cell morphology, antibiotic sensitivity, and eventually viability of the bacterium. (Korean J Oral Maxillofac Radiol 2007; 37: 35-43)

자궁경부암 선별검진을 위한 세포진 검사와 인유두종바이러스 검사의 임상적 유용성

안태규,이경,김동원,한세준 대한부인종양 콜포스코피학회 2002 Journal of Gynecologic Oncology Vol.13 No.4

목적 : 자궁경부 세포진 검사와 HPV DNA (Hybrid capture 1) 검사의 임상적 유용성을 알아보고자 본 연구를 시행하였다. 연구 방법 : 1995년 12월 1일부터 1998년 3월 31일까지 조선대학교병원 산부인과 외래를 방문한 1028명의 환자중 질확대경을 통한 조직검사 및 수술을 시행한 후 조직 검사를 시행하여 결과 비교가 가능한 환자 305명을 대상으로 본 연구를 시행하였다. 결과 : 1. 세포진 검사의 민감도는 63.0%, 특이도는 88.1%, 양성 예측율은 89.7%, 음성 예 측율은 59.3%이었다. 2. HPV DNA 검사의 민감도는 51.9%, 특이도는 85.3%, 양성 예측율은 85.2%, 음성 예측율은 52.1%이었다. 3. HPV DNA검사와 세포진 검사를 병행하였을 경우 민감도는 76.9%, 특이도는 76.9%, 위양성률은 23.1%, 위음성률은 23.1%이다. 결론 : 본 연구에서 자궁경부암 선별 검사로서 형태학적 검사인 세포진 검사와 생물학적 검사인 HPV DNA 검사를 동시에 사용하면 서로의 단점을 보완하여 향후 적절한 선별검진의 간격을 결정하는 등 자궁경부암 조기 진단에 매우 유용하게 사용할 수 있을 것으로 생각된다. Objective : The purpose of this study was to find out the efficacy of cytology and HPV DNA test (Hybrid capture I) in early diagnosis of cervical cancer. Methods :This study included 1028 patients who visited the Department of Obstetrics and Gynecology, Cho-sun University Hospital From December 1, 1995 to March 31, 1998. And we chose 305 samples who underwent biopsies among taking the cytology and HPV DNA test. All women simultaneously underwent an exo- and endo-cervical smear. Results : 1. The sensitivity and specificity of cytology were 63.0% and 88.1%, respectively. 2. The positive predictive value and negative predictive value of cytology were 89.7% and 59.3%, respectively. 3. The sensitivity and specificity of HPV DNA test were 51.9% and 85.3%, respectively. 4. The positive predictive value and negative predictive value of HPV DNA test were 85.2% and 52.1%, respectively. 5. When HPV DNA test and Papanicloaou smear were combined, the sensitivity and specificity were 76.9% and 76.9%, respectively. However, the specificity of the combined test with HPV DNA test and Papanicloaou smear was lower than Papanicloaou smear alone. Conclusion : The sensitivity of cervical cytology was markedly improved by combination with HPV DNA test. So, the combined test could improve the high false negativity of cervical cytology, if the effectiveness is confirmed by mass screening study.

고실성형술에서 Propofol-Remifentanil을 이용한 전정맥마취와 Sevoflurane을 이용한 흡입마취의 비교

안태훈,안태규,연화 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2

Background: Total intravenous anesthesia (TIVA) using propofol and remifentanil was compared with inhalation anesthesia using sevoflurane in patients undergoing tympanoplasty. The aim of the present study was to compare hemodynamic changes during induction and maintenance of anesthesia as well as the rates of induction and recovery following each anesthetic technique. Methods: Thirty patients (ASA physical status 1, 2) scheduled for elective surgery were divided randomly into 2 groups. Group I (propofol-remifentanil) was induced and maintained with propofol 3.0 μg/ml and remifentanil 0.2 μg/kg/min. Group Ⅱ (sevoflurane) was induced with thiopental sodium 5 mg/kg and maintained with 2.0 vol% sevoflurane. The patient's blood pressure and heart rate were measured before and after tracheal intubation, skin incision, and every 30 minutes interval. The patient's ability to recovery of the spontaneous breathing, and open their eyes and respond to simple verbal commands was assessed at 15 to 30 seconds intervals after discontinuation of the anesthetics. Results: The systolic and diastolic blood pressure and heart rate after intubation were increased in groups Ⅱ, and were unchanged in groups I as compared to preintubation values. Emergence times were significantly shorter in group I than group Ⅱ. Conclusion: We concluded that TIVA using propofol and remifentanil can provide hemodynamic changes and rapid emergence than inhalation anesthesia using sevoflurane.

금연이 재입원한 정신병 환자의 약물용량에 미치는 영향에 대한 예비 연구

박규태,신정호,안정숙 大韓神經精神醫學會 1997 신경정신의학 Vol.36 No.6

본 연구는 30명의 정신병환자를 대상으로 하여 동일환자에서 일정 간격을 두고 원주기독병원 정신과 병동에 흡연이 허용되던 시기에 입원했을 때와 금연병동 때 재입원했을 때 투약된 항정신병약물 용량과 항정신병약물의 부작용에 따른 benztropine과 propranolol 처방용량을 비교하여 다음과 같은 결과를 얻었다. 1) 평균 입원기간은 금연병동 입원동안은 46.0일로 흡연병동 입원동안의 61.1일과 비교하여 감소되었다. 평균 입원기간은 통계적으로 유의하게 감소되었고(p<0.05). 약 2/3의 환자가2일에서 92일간의 입원기간의 감소가 있었다. 2) Chlorpromazine 등가량으로 환산한 항정신병약물 용량은 흡연병동 입원시기 동안의 395.0mg과 비교하여 금연병동 재입원시기 동안에는 296.8mg으로 통계적으로 유의하게 낮았다(p<.01). 3) 정신분열증 환자(n=13)에게 투약된 benztropine 용량은 금연병동 재입원시기 동안에는 0.56mg으로 흡연병동 입원 동안의 0.14mg과 비교하여 통계적으로 유의하게 용량이 높았다(p<.05). 4) Propranolol 용량은 흡연병동 입원동안과 금연병동 재입원 동안에 유의한 차이가 없었다. 결론적으로, 흡연해온 정신병 환자는 금연함으로써 입원기간의 감소가 있었으며. 항정신병약물 용량이 줄어드는 반면 정신분열중 환자에서는 추체외로계 증상은 증가할 가능성이 있으므로 이에 대한 대비가 있어야 할 것이다. 그러므로 환자의 흡연상태는 항정신병약물의 용량과 부작용에 관한 고려에서 반드시 평가해야 할 중요한 변수중 하나임을 강조하고자 한다. This study was designed to investigate the effect of no-smoking on the pattern of medication for 30 re-admitted psychotic patients in the no-smoking(smoking-prohibited) ward, who had been admitted and medicated previously in the smoking(smoking-allowed) ward, in Wonju Christian Hospital, by comparing daily mean dosage of antipsychotics, benztropine mesylate, and propranolol administered in smoking state and in no-smoking state of the same patients. The results were as fo11ows : 1) The mean hospital stay was reduced to 46.0 days in no-smoking ward compared to 61.1 days in smoking-allowed ward. This means mean admission period was reduced significantly(p<0.05) and over two thirds of all subjects experienced reduction of hospital stay from 2 days to 92 days. 2) A statistically significant difference was found in the dosage of antipsychotics in all subjects. Calculated in terms of chlorpromazine equivalent the daily mean was 296.8 ± 180. 7mg in no-smoking state, and.395.0±232.2mg in smoking state(p <.01). 3) In schizophrenics(n= 13), the daily mean dosage of benztropine mesylate was 0.56±0.62mg in no-smoking state and 0.14±0.21mg in smoking state, showing a significant difference(p <.05). In bipolar group, the mean dosage in no-smoking ward was also smaller than in smoking ward, though not significant. 4) The difference of the daily mean dosage of propranolol between two states was not significant. In conclusion, in no-smoking state psychotic patients required less hospital stays and significantly lower dosage of antipsychotics, and the schizophrenics required significantly higher dosage of benztropine mesylate for extrapyramidal symptoms. The authors suggest that psychiatric patients should be encouraged to stop smoking and recommend that psychiatrists monitor smoking state of their psychotic patients for proper and efficient drug therapy.

대류난방시 상하온도차가 인체의 온열 쾌적성에 미치는 영향

김동규,김광호,김경철,박태안,박희욱 동의공업대학 2000 論文集 Vol.26 No.1

Purpose of this study is first to show the effects of vertical air temperature differences on thermal comfort and physiological responses, and second to examine the acceptable vertical air temperature differences. In this experiments, 12 subjects (6 males and 6 females) were exposed at 12 test conditions. The subjects voted each 10 min, about his thermal feeling, and 7 skin surface temperature, the air temperature were measured every 20 second. From this experiments, it was found that the upper part temperature affects the thermal sensation more strongly than lower part temperature. In winter, the acceptable temperature differences drawn by this study are 3℃ in the case that the upper part temperature is higher than the lower part temperature.

Phosphamidon 분해세균의 분리동정 및 생분해능

강양미,허성남,안태석,송홍규 江原大學校 附設 環境硏究所 1999 環境硏究 Vol.16 No.-

토양으로부터 유기인계 살충제인 phosphamidon을 분해하는 세균들을 분리하고 Biolog system을 이용하여 동정하였다. 그람양성 세균들은 모두 Bacillus 속에 속하는 종들이었으며 그람음성 세균들은 토양에서 우점하지 않는 세균들이 많았다. 이들중 phosphamidon 함유배지에서 생장률이 높은 균주들을 선택하여 phosphamidon 분해능을 조사한 결과 Capnocytophaga gingivalis로 동정된 YD-17 균주가 가장 높은 생분해능을 나타내어 1000 ppm의 phosphamidon이 배양 21일 후 9.4%의 잔류량을 보였으며 이는 대조구에 비해 제거율이 52% 증가된 결과였다. 이 때 균주의 생장을 단백질량으로 측정하였는데 분해균주들이 고농도의 phosphamidon에 의해 저해되지 않고 지속적인 생장을 하여 phosphamidon을 탄소원으로 이용하는 생분해가 일어난 것을 확인할 수 있었다. Organphosphorus insecticide phosphamidon-degrading bacteria were isolated from agricultural soils and identified using Biolog microtiter assay. All Gram-ositive degrading bacterial strains belong to genus Bacillus and many Gram-negative bacteria were rare soil species. Among them fast growing strains on phospamidon-containing minimal medium were selected and their biodegrading capability were measured. YD-17 which was identified as Capnocytophaga gingivalis showed the highest biodegradation rate. It could increase the removal of phosphamidon up to 52%. During the biodegradation continuous increase of amount of cell protein was observed, which, indicated that phosphamidon was utilized as a carbon source for phosphamidon-degrading bacteria.

자궁탈의 임상적 고찰

김동원,노형균,안태규,최상준,송창훈,정혁 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.1

The purpose of this study was to evaluate clinical aspects of prolape uteri. This clinical and statistical study was attempted to analyze 369 cases of uterine prolapse(328 cases -operated, the other cases - not operated) from Jan. 1994 to Dec. 1998 at the Department of Obstetrics and Gynecology, Chosun University Hospital. The results were as follows. 1. The incidences of uterine prolapse was 0.62% of the total admitted gynecologic patients during the study period. 2. Age distribution at the time of operation showed that 184 cases(25.6%) were 60 to 65 years of age, and 62 cases(18.2%) were 56 to 60 years of age. 3. Age distribution at the time of development of prolapse uteri showed that 134 cases(36.3%) were 21 to 30 years of age, 126 cases(34.1%) were 31 to 40 years of age. 4. 228 cases(61.7%) were those who had suffered from uterine prolapse for more 11 years and the mean durations were 15.6 years. 5. The degree of prolapse uteri showed that 281 cases(76.1%) were 3rd degree and 61 cases(16.5%) were 2nd degree. 6. The parity showed that 297 cases(80.2%) were over 4 times and 2 cases(0.27%) was nullipara. 7. The delivery place was at home in 318 cases(86.1%) and in hospital in 51 cases(13.9%). 8. The main symptoms comprised prolapse in 311 cases(84.2%), urinary frequency in 259 cases(70.1%), and bearing down sensation in 242 cases (65.8%). 9. An operation in 328 cases was carried out of 369 cases of prolapse uteri, 301 cases were treated by the vaginal hysterectomy with either anterior &osterior repair or posterior repair, 23 cases were treated by anterior &osterior repair. 10. The postoperative complications were urinary tract infection in 7 cases(2.1%), urinary retention in 4 cases(1.2%), vaginal vault prolapse in 2 cases(0.6%). 11. The indwelling foley catheter was kept for 7 days in 279 cases and the average postoperative hospitalization was around 9 days. 12. The postoperative pathologic findings were chronic cervicitis in 211 cases(67.8%), squamous metaplasia of the cervical gland in 98 cases(31.5%), adenomyosis in 69 cases, leiomyoma in 47 cases, and dysplasia of cervix, cervical polyp, endometrial polyp were also resulted. In conclusion, clinical features of uterine prolapse were studied. The clinical evaluation of uterine prolapse include age, parity, degree of prolapse, delivery site, age of onset, symptomatology, type of operation, complications, the results of histopathological examinations. Further study is necessary before these aspects can be implicated in the clinical evaluation of uterine prolapse.

The Effect of Estrogen and Progestogen in Breast Cancer

( Tae Gyu Ahn ) 대한산부인과학회 2012 서울심포지움 Vol.17 No.-

Preeclampsia and aspirin

( Tae Gyu Ahn ),( Jong Yun Hwang ) 대한산부인과학회 2023 Obstetrics & Gynecology Science Vol.66 No.3

Preeclampsia (PE) is a multisystem disorder that is an important cause of maternal and perinatal deaths. Currently, delivery is the only final treatment for PE. This practice is usually accompanied by premature birth, which inevitably increases neonatal morbidities. Aspirin is a non-selective non-steroidal anti-inflammatory drug that irreversibly inhibits cyclooxygenase enzymes involved in converting arachidonic acid to prostaglandins and thromboxane. Aspirin inhibits thromboxane A2 production via platelet aggregation, thereby increasing the prostacyclin/thromboxane A2 ratio and reducing platelet aggregation. Since the first case report of aspirin’s potential use during pregnancy was reported in 1978, many studies have attempted to confirm the effect of aspirin on PE, and the results have been controversial. However, this preventive strategy is generally accepted in clinical practice. As evidence for aspirin’s prevention of PE has been accumulating, a recent study investigated the effectiveness of aspirin at high doses of 150 mg, which is higher than before. However, there is an ongoing debate about how much aspirin should be used during pregnancy and when to start aspirin therapy. Guidelines for the use of prophylactic aspirin during pregnancy vary slightly among countries and groups. In this article, we review and summarize the evidence regarding the use of aspirin for PE prevention.

Simultaneously estimation of the HPV E2/E6 DNA ratio by multiplex PCR in cervical preneoplastic and neoplastic Lesions

( Tae Gyu Ahn ),한세준 ( Sei Jun Han ),( Byoung Rai Lee ) 대한산부인과학회 2004 대한산부인과학회 학술대회 Vol.90 No.-