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Sung Weon Ryoo,Young Kil Park,Sue-Nie Park,심영수,류현정,강성만,Gill-Han Bai 한국미생물학회 2007 The journal of microbiology Vol.45 No.3
In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDITOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.
Ryoo, Sung-Weon,Park, Young-Kil,Park, Sue-Nie,Shim, Young-Soo,Liew, Hyun-Jeong,Kang, Seong-Man,Bai, Gill-Han The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.3
In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDI-TOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.
( Ji Hoon Ryoo ),( Seon Hwa Lee ),( Hyun Ji Lee ),( Joonsoo Park ),( Sung-ae Kim ),( Young Wook Ryoo ),( Dong Hoon Shin ),( Moo Kyu Suh ),( Jun Young Kim ),( Kyung Duck Park ),( Weon Ju Lee ),( Seok-j 대한피부과학회 2021 Annals of Dermatology Vol.33 No.3
Background: Only a few studies have tried to assess factors relevant to the satisfaction of the participants in atopic dermatitis (AD) educational programs. More systematic modeling of this issue is needed. Objective: To examine the benefit of a conjoint educational program for AD on patients and caregivers in a clinical setting. Methods: In a half-day educational program called “AD school”, 831 people (493 patients and 338 family members) participated for 8 years. Various educational and entertaining programs were provided. The on-site survey was administered to measure participants’ satisfaction and perception of the benefit. We applied structural equation modeling to identify the relations among satisfaction and perception. Results: A total of 209 family survey data was obtained and analyzed. The survey items were grouped into four categories. The categories were classified as individual education, group education, fun activity, and overall satisfaction (fun, benefit, intention to re-join and recommend to others). According to the model that we built, comprehensive group education was demonstrated to be the most relevant factor affecting overall satisfaction. Conclusion: Our holistic approach would allow dermatologists to improve the efficacy of the conjoint educational program for AD. (Ann Dermatol 33(3) 237∼244, 2021)
( Hee Yoon Kang ),( Sung Weon Ryoo ),( Young Kil Park ),( Woo Jin Lew ) 대한결핵 및 호흡기학회 2009 Tuberculosis and Respiratory Diseases Vol.67 No.6
Background: Mycobacterial Interspersed Repetitive Units (MIRUs) that are located mainly in intergenic regions dispersed throughout the Mycobacterium tuberculosis genome. The selected MIRU loci, which were composed of a 12-locus set, demonstrated a high power for discrimination of Mycobacterium tuberculosis isolates collected from Kangwon province of Korea. To evaluate its ability to discriminate the M. tuberculosis strains, 45 clinical isolates were genotyped using the methods IS 6110 RFLP and MIRU. Methods: All the samples were collected during the period from January 2007 to December 2007 from TB patients, who were residents and registered to a public health center of Kangwon Province in Korea. A total of 45 DNAs were extracted from clinical isolated mycobacterial strains and genotyped using IS 6110 RFLP, the MIRU method. Results: We compared the 12-MIRU with IS 6110 RFLP in the 45 samples, the 12-locus version offered less discriminatory power (Hunter-Gaston discriminatory index [HGDI]: 0.959 vs 0.998; 57.78% of clustered cases vs 8.89%). Conclusion: This 12-locus MIRU can be useful when additional combinations of other loci for genotyping M. tuberculosis in Korea where the Beijing family strains are dominant.
유향복(Hyang-Bok Ryoo),김도원(Do-Weon Kim),정상국(Sang-Gug Jeong),최종길(Jong-Gil Choi),나현(Hyun Na),이종아(Jong-A Lee) 한국철도학회 2013 한국철도학회 학술발표대회논문집 Vol.2013 No.5
기존선의 경우 수송능력 향상과 친환경 녹색철도 구현을 위해 점차적으로 고속화를 추진하고 있으며, 이에 따른 속도향상과 유지보수 편리성 등을 고려하여 이중에어섹션을 설치하는 추세이다. 그러나 전차선분야와 신호분야가 개별 설계를 하면서 절연구간내의 표지와 신호기간 이격거리를 적정하게 설계하지 못하여 현장에서 시공중에 변경하는 사례가 발생하고 있다. 또한 역행지점의 통과속도에 대한 기준이 없어 설계자나 검토자의 성향에 따라 임의적으로 적용되고 있는 실정으로 이러한 문제를 해소하고 설계기준을 마련할 필요성이 있다. 본 연구에서는 절연구간의 시설기준 및 현장사례조사, 선로조건과 차량별 특성을 분석하여 절연구간에서의 열차 타행가능거리와 속도를 산정하여 설계기준을 제안하였다. It is expected that conventional lines are reconstructed gradationally to speed up for increasing transport capability and eco-friendly railway. Based on this trend, double air section as a insulation system is usually installed in the middle of line due to a positive reason in terms of speed and convenience in maintenance. However designs of signs and distance between signals are not studied properly both in catenary and signal field causing changing contract during the construction. The results are always different depending on values or reference designer apply because there is not proper reference of speed at power-running area near by neutral-section. So design reference should be required for getting rid of those errors. An existing installation reference of sectioning area, case happened on sites, line conditions and various train characters are studied and we finally suggested design reference based on the calculation of available running distance in coasting-running mode and speed going trrough neutral-section.
Exploitation of Culture Medium for Mycobacterium tuberculosis
Kim, Hye-Jin,Ryoo, Sung-Weon 대한미생물학회 2011 Journal of Bacteriology and Virology Vol.41 No.4
The culture media for Mycobacterium tuberculosis (MTB) were contrived from both egg-based and agar based ingredients. In 1903, Dorset introduced the first egg-based medium. It was followed by the invention of L$\ddot{o}$wenstein-Jensen and Ogawa media that contain whole eggs as nutrient and malachite green to inhibit the growth of contaminants. These media have been used worldwide for their usefulness and inexpensiveness. However they have a fundamental disadvantage that the cultivation time for mycobacterial growth takes more than 4 weeks. In 1947, Dubos introduced the first agar medium and followed by the invention of the 7H10 by Middlebrook and Cohn. A powder base of these media contains agar, combination of seven salts, L-glutamic acid, pyridoxine, biotin, and malachite green. This requires the addition of the oleic acid, albumin, dextrose, and catalase (OADC) growth supplement. BACTEC MGIT960 has recently been introduced for rapid cultivation of MTB, which is fully automated liquid culture system with modified 7H9 broth. Agar-based medium developed by Middlebrook has a number of advantages over egg-based medium. One of them is transparency, which enables earlier detection of growing colonies. The major disadvantages of agar media are the high cost of OADC and the need for a $CO_2$ incubator. In conclusion, there is a need for a new agar medium, which can be produced at a lower cost and earlier growth detection. In this review, we introduced the growth promoting factors which can be used as an alternative new growth supplement, cAMP and resuscitation-promoting factor (Rpf). These factors may abrogate a lag in adaption of the bacilli in culture media.
SWATH-based Comparative Proteomic Analysis of the Mycobacterium bovis BCG-Korea Strain
Lee, Won-Kyu,Baek, Je-Hyun,Ryoo, Sung Weon,Yu, Yeon Gyu Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.3
A derivative of Mycobacterium bovis Bacillus Calmette-Guerin (BCG) has been used for the preparation of tuberculosis vaccines. To establish a Korean tuberculosis vaccine derived from BCG-Pasteur $1173P_2$, genome sequencing of a BCG-Korea strain was completed by Joung and coworkers. A comparison analysis of the genome sequences of the BCG-Pasteur $1173P_2$ and BCG-Korea strains showed marginal increases in the total genome length (~0.05%) and the number of genes (~4%) in the BCG-Korea genome. However, how the genomic changes affect the BCG-Korea protein expression levels remains unknown. Here, we provide evidence of the proteomic alterations in the BCG-Korea strain by using a SWATH-based mass spectrometric approach (Sequential Window Acquisition of all THeoretical mass spectra). Twenty BCG proteins were selected by top-rank identification in the BCG proteome analysis and the proteins were quantified by the SWATH method. Thirteen of 20 proteins showing significant changes were enough to discriminate between the two BCG proteomes. The SWATH method is very straightforward and provides a promising approach owing to its strong reliability and reproducibility during the proteomic analysis.
Baig, Irshad Ahmed,Moon, Ji-Young,Lee, Sang-Choon,Ryoo, Sung-Weon,Yoon, Moon-Young Elsevier Science Publishers B.V 2015 Biochimica et biophysica acta. Proteins and proteo Vol.1854 No.10
<P>Acetohydroxyacid synthase (AHAS) from Mycobacterium tuberculosis (Mtb) is a promising potential drug target for an emerging class of new anti-tuberculosis agents. In this study, we identify short (30-mer) single-stranded DNA aptamers as a novel class of potent inhibitors of Mtb-AHAS through an in vitro DNA-SELEX method. Among all tested aptamers, two candidate aptamers (Mtb-Apt1 and Mtb-Apt6) demonstrated the greatest inhibitory potential against Mtb-AHAS activity with IC50 values in the low nanomolar range (28.940.002 and 22.350.001nM respectively). Interestingly, inhibition kinetics analysis of these aptamers showed different modes of enzyme inhibition (competitive and mixed type of inhibition respectively). Secondary structure-guided mutational modification analysis of Mtb-Apt1 and Mtb-Apt6 identified the minimal region responsible for their inhibitory action and consequently led to 17-mer and 20-mer shortened aptamers that retained equivalent or greater inhibitory potential. Notably, a modeling and docking exercise investigated the binding site of these two potent inhibitory aptamers on the target protein and showed possible involvement of some key catalytic dimer interface residues of AHAS in the DNA-protein interactions that lead to its potent inhibition. Importantly, these two short candidate aptamers, Mtb-Apt1 (17-mer) and Mtb-Apt6 (20-mer), also demonstrated significant growth inhibition against multidrug-resistant (MDR-TB) and extensively drug-resistant (XDR-TB) strains of tuberculosis with very low MIC of 5.36μg/ml and 6.24μg/ml, respectively and no significant cytotoxicity against mammalian cell line. This is the first report of functional inhibitory aptamers against Mtb-AHAS and provides the basis for development of these aptamers as novel and strong anti-tuberculosis agents.</P>