Soymilk를 이용한 요구르트 제조 및 저장기간 동안의 품질변화에 관한 연구

신상민 ( Sang Min Shin ),송광영 ( Kwang Young Song ),서건호 ( Kun Ho Seo ),윤여창 ( Yoh Chang Yoon ) 한국유가공기술과학회 2012 한국유가공기술과학회지 Vol.30 No.2

This study was performed for analyzing the general composition and the change in the quality of soymilk-derived yogurts manufactured by adding skim milk and whey powder to soymilk heat-treated at 95℃/5 min and 120℃/10 min, respectively. 1. During the storage of soymilk yogurt, the concentrations of total solids, protein, fat, and lactose slightly decreased, whereas viscosity, content of ash and NPN, and the number of lactic acid bacteria remained unchanged. 2. The pH and titratable acidity changed rapidly in all soymilk yogurts after 3 h of incubation. 3. We found 7.8×108 lactic acid bacteria in the control sample, 4.7×108 and 5.02×108 in soymilk yogurt with skim milk, respectively, and 5.9×108 and 5.5×108, respectively in soymilk yogurt with whey powder according to degree of heat treatment with 95℃/5 min and 120℃/10 min. 4. The viscosity of yogurt samples became lower as the heat treatment increased in temperature and in the length of time. 5. The value of sensory evaluation was relatively high in soymilk yogurt with the added skim milk, which was heat-treated 95℃/5 min; however, the value was significantly lower than that of the control sample. 6. Lactose, glucose, and galactose were detected in all samples because lactose is degraded into glucose and galactose within 3 h of inoculation.

총경동맥 폐쇄시간에 따르는 국소 뇌혈류 변화 : 실험적 연구 An Experimental Cat Model

강준기,성태경,조병일,백민우,김문찬,허춘웅,하영수,송진언 대한신경외과학회 1983 Journal of Korean neurosurgical society Vol.12 No.3

The microvasculature of the brain is also quite susceptible to ischemic insult, and substantial portions of the brain are not reperfused after restoration of the blood supply following overtime of critical ischemic periods. The purpose of this series of experiments was to determine the effects of ischemia on subsequential regional cerebral blood flow measurements and cortical electric activities following reperfusion after ischemia and also to define the proper time of vascular occlusion without irreversible neural damage. Cerebral ischemia was induced in cat by bilateral common carotid occlusions for periods of 10, 30, to 60 minutes, and the blood supply was reperfused for 3 hours after clamp-off. Regional cerebral blood flow(rCBF) was measured by hydrogen clearance technique following ischemia, restoration of blood supply and electroencephalogram recovery could be predicted according to the rCBF. Forty adult cats weighing 2.7 to 4.0㎏ were used in this study. The animals were divided into 4 groups of 10 cats each : normal control, 10 min-clamped, 30 min-clamped, and 60 min-clamped groups. The results obtained were as follows : 1) The mean rCBF was 24.6±7.0㎖/100g/min in control group. 2) Bilateral carotid occlusions resulted in a reduction of the rCBF(12.4±4.1㎖/100g/min) to 50% of control flow on both hemispheres. 3) Sequential changes of the rCBF after reperfusion : (1) There was restored the rCBF(21.3±5.1㎖/100g/min) to control flow in the 10 minutes-clamped group. (2) There was a 85% recovery of control flow in the 30 minutes-clamped group. (3) There was a only 25% recovery of control flow in the 60 minutes-clamped group. 4) A close correlation was found between cortical electrical activity and rCBF suggesting a threshold relationship. (1) The changes of cortical electric activity began to notice at rCBF less than 17.4±4.7㎖/100g/min. (2) The recovery of cortical electric activity noted at rCBF more than 10.2±2.3㎖/100g/min. 5) There was no evidence of ischemic involvement at the cortex, white matter and basal ganglia in the 10 minutes clamped group, but demonstrated a dense wedge shaped infarct at the cortex and uncus herniation in the 60 minutes clamped group. The rCBF and cortical electric activity restored to normal values in reperfusion within 10 minutes after occlusion of both common carotid arteries.

인체혈장 중 에탐부톨의 HPLC 분석법의 검증 및 단일용량 투여에 의한 약물동태 연구

곽혜선,박경호,최준식,송진아,성민경,장정옥,이화정 한국약제학회 2005 Journal of Pharmaceutical Investigation Vol.35 No.2

An HPLC method was employed for the determination of ethambutol in human plasma. After addition of internal standard (IS, octylamine, 2 μg/mL) and alkalinization of the plasma with 5 M sodium hydroxide, the drug and IS were extracted into the mixture of chloroform and diethyl ether (40:60, v/v). Following a 15-min vortex-mixing and a 10-min centrifugation, the organic phase was spiked with 100 pL of phenylethylisocyanate (2000 μg/mL) for chemical derivatization, mixed for 5 min and evaporated to dryness under a stream of nitrogen. The residue was reconstituted with 100 μL of mobile phase and 20 pL was injected into Cl8 column with a mobile phase consisting of methanol:water (70:30, v/v). The samples were detected utilizing an ultraviolet detector at 200 nm. The method was specific and validated with a limit of 0.15 μg/mL. Infra- and inter-day precision and accuracy were acceptable for all quality control samples including the lower limit of quantification. The applicability of this method was demonstrated by analysis of human plasma after oral administration of a single 1200-mg dose to 20 healthy subjects. From the plasma ethambutol concentration vs. time curves, the mean AUC was 9.61 ± 1.64 μg hr/mL. and Cmax of 2.68 μg/mL reached 2.73 hr after administration. The mean biological half-life of ethambutol was 3.46 ± 1.21 hr. Based on the results, this simple and validated assay could readily be used in any pharmacokinetic studies using humans.

Bioconversion of ginsenoside Rc into Rd by a novel α-L-arabinofuranosidase, Abf22-3 from Leuconostoc sp. 22-3: cloning, expression, and enzyme characterization.

Liu, Qing-Mei,Jung, Hae-Min,Cui, Chang-Hao,Sung, Bong-Hyun,Kim, Jin-Kwang,Kim, Song-Gun,Lee, Sung-Taik,Kim, Sun-Chang,Im, Wan-Taek N.V. Swets en Zeitlinger 2013 Antonie van Leeuwenhoek Vol.103 No.4

<P>A novel α-L-arabinofuranosidase (Abf22-3) that could biotransform ginsenoside Rc into Rd was obtained from the ginsenoside converting Leuconostoc sp. strain 22-3, isolated from the Korean fermented food kimchi. The gene, termed abf22-3, consisting of 1,527 bp and encoding a protein with a predicted molecular mass of 58,486 Da was cloned into the pMAL-c2x (TEV) vector. A BLAST search using the Abf22-3's amino acid sequence revealed significant homology to that of family 51 glycoside hydrolases. The over-expressed recombinant Abf22-3 in Escherichia coli BL21 (DE3) catalyzed the hydrolysis of the arabinofuranoside moiety attached to the C-20 position of ginsenoside Rc under optimal conditions of pH 6.0 and 30 C. This result indicated that Abf22-3 selectively converts ginsenoside Rc into Rd, but did not catalyze the hydrolysis of glucopyranosyl groups from Rc or other ginsenosides such as Rb1 and Rb2. Over-expressed recombinant enzymes were purified by two steps with amylose-affinity and DEAE-cellulose chromatography and then characterized. The kinetic parameters for α-L-arabinofuranosidase showed apparent Km and Vmax values of 0.95 0.02 μM and 1.2 0.1 μmol min(-1) mg of protein(-1) against p-nitrophenyl-α-L-arabinofuranoside, respectively. Using a purified MBP-Abf22-3 (10 μg/ml), 0.1 % of ginsenoside Rc was completely converted to ginsenoside Rd within 20 min.</P>

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

특수교육지원센터 치료지원서비스에 대한 견해 : - 작업치료 서비스를 받는 보호자를 중심으로 -

송민옥,이성자,김훈주 대한감각통합치료학회 2010 대한감각통합치료학회지 Vol.8 No.1

목적 : 본 연구에서는 특수교육지원센터에서 시행되는 치료지원서비스의 인식, 목적, 특징 및 개선방안을 살펴봄으 로써 치료지원서비스의 전반적 실태를 파악하고 이를 통해 최적의 치료지원서비스를 제공하기 위한 기초자료로 제시하고자 한다. 연구 방법 : 현재 특수교육지원센터에서 치료지원서비스를 받고 있는 치료지원대상 아동의 보호자를 대상으로 개별 인터뷰가 진행되었다. 인터뷰는 평균 40분이 소요되었으며, 모든 인터뷰는 녹취되었다. 결과 : 치료지원서비스에 대한 근본적인 인식이 부족하였고, 치료영역의 전문가가 치료를 담당함으로 인하여 치료사에 대한 신뢰도가 높은 것으로 조사되었다. 또한 치료실의 시설 및 교재교구의 다양성으로 인해 질적으로 높은 치료를 받는다고 생각하고 있었으며 모든 연구대상자는 무상교육을 치료지원서비스의 특징으로 꼽았다. 결론 : 작업치료사가 특수교육지원센터에서 치료지원서비스 전문가로 자리 매김하기 위해서 많은 노력과 연구 및 대외적인 홍보가 필요하다. Objective : The purpose of this study is to provide practical guidelines for the better performance of the Occupational therapy services(OT services) in the Supporting Center for Special Education. The study is based upon the quality and satisfaction of the OT services, measured by interviewing parents whose children have taken the services provided by Ulsan Kang-Nam Supporting Center for Special Education. Methods : Five parents for the children having OT services since March, 2010 in the Kang-Nam Supporting Center were interviewed individually. The interview per each person took thirty to fifty minutes and was all audio-recorded. Results : The satisfaction of the qualification for the OT therapists working at the Kang-Nam Supporting Center was high. The quality for treatment facilities and the variety for treatment materials met the expectation for OT to be well-performed. The free OT treatment system was the one of significant features for the OT services provided by the Kang-Nam Supporting Center. However, the perception of the interviewed parents for the OT was very low.

高品質 豚肉 生産을 위한 고구마 사일리지의 開發에 關한 硏究 : 3. 肥肉豚에 대한 고구마 사일리지 給與가 豚肉의 品質에 미치는 影響 3. Effects of Supplementing the Sweet Potato Silage on Pork Quality

安瑛敏,高永杜,文勝式,陳詳根,鄭孟和 진주산업대학교 농업기술연구소 1999 農業技術硏究所報 Vol.12 No.-

육에 대한 소비자들의 욕구는 계속해서 증가하고 다양해지고 있어 이러한 욕구에 부응하기 위해 육의 풍미 및 육색을 증진시키는 것으로 알려진 고구마 사일리지를 급여한 돈육 일반성분과 물리적인 성질 및 도체특성을 조사하였다. 고구마 사일리지 제조는 고구마의 외부에 부착된 흙을 수세하여 마쇄한 후 고구마 사일리지 1은 고구마 70%, 맥강 20%, 대두박10%를 혼합하여 제조하였으며, 고구마 사일리지 2는 고구마 70%, 맥강 30%를 혼합하여 제조하였다. 일반성분으로는 수분, 조지방, 조단백, 조회분을 조사하였고, 물리적 성질로는 pH, 보수성, 연도, 육과 지방색, 관능검사, 도체특성을 조사하였다. 일반성분에서 수분은 고구마 사일리지 2 급여구가 다른 처리구에 비하여 유의적으로 높게 나타난(P<0.05) 반면에 조지방 함량과 조단백질 함량은 유의적으로 낮은 함량을 보였으며, 그리고 조회분 함량은 처리구간에 유의적인 차이가 없었다. 물리적 성질인 pH의 변화는 대조구와 고구마 사일리지 급여구간에는 유의적인 차이가 없었다. 보수성은 고구마 사일리지 급여구가 대조구보다 유의적으로 높게 나타났으며(P<0.05), 연도는 고구마 사일리지 급여구가 대조구에 비하여 좋은 결과를 보였다. 육색의 변화는 고구마 사일리지 급여로 명도가 유의적으로 낮게 나타났으며, 적색도와 황색도는 측정부위에 따라 값의 변화가 심하였다. 지방색은 고구마 사일리지 급여가 대조구에 비하여 황색도가 낮아 좋은 결과 였다. 관능검사 결과는 고구마 사일리지 급여가 대조구에 비하여 풍미는 유의적으로 높은 성적을 보였지만(P<0.05), 다즙성, 견고성, 탄력성에는 유의적인 차이가 없었다. 전체적인 관능검사 성적은 고구마 사일리지 2 급여구가 가장 좋은 성적을 얻었다. 도체특성에 미치는 변화를 측정한 결과 도체중은 유의적으로 낮게 나타났으나(P<0.05), 지육율은 차이가 없었다. 그러나 고구마 사일리지 급여로 등지방 두께가 유의적으로 감소하는 결과를 보였다(P<0.05). 본 실험 결과 보수성과 연도는 대조구에 비해 고구마 사일리지 급여구가 더 나은 결과 나타내었으며, 육색과 관능검사, 도체특성 결과도 대조구에 비교해 고구마 사일리지 급여구가 유의적으로 더 나은 것으로 나타나 고구마 사일리지를 급여시 특히 고구마 사일리지 1 급여구의 경우 돈육의 육질을 크게 향상시킬 수 있을 것으로 사료된다. The present study was conducted to investigate the effect of supplementary feeding of sweet potato silage on carcass and its meat quality in finishing gilts. Two sweet potato silages containing same ingredients with different ratios(silages 1 and 2; 70 and 70% sweet potato, 20 and 30% barley bran, 10 and 0% soybean meal, respectively) were fermented for 21 days in 500 kg-capacity silos. Finishing gilts were fed a commercial finishing diet(CONT), or the same basal diet plus 0.4kg silage 1(TRT 1) or silage 2(TRT 2)/head/day beginning from 90kg of body weight, 18 heads per group, up to approximately 110kg of final weight and slaughtered. After measuring carcass traits, loin eye sections were removed and their physicochemical characteristics were analyzed. The sensory evaluation on the fresh and cooked meat was performed by the panel test and the whole experiment was repeated 3 times. Backfat thickness was reduced in TRT 1 and TRT 2 compared with CONT, but carcass weight and dressing percentage were not different between the feeding regimens. Compared with CONT, TRT 1 and TRT 2 had greater water holding capacity, tenderness, fat color and palatability scores including flavor and reduced brightness, with no difference in pH, juiciness, firmness and gumminess. Overall, TRT 2 exhibited the greatest sensory evaluation score.

건설 프로젝트에서 D-virus가 안전에 미치는 영향에 관한 기초적 연구

송진우,표영민,진유나,이상범,김정길 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

Construction site has contributed to construction productivity improvement by utilizing information, simplification, and standardization concept. Also it has contributed to quality and productivity improvement by standardizing design and construction process, using them to a fair decision-making process by organizing diverse data in construction process. However, construction process has diverse variables and uncertain case due to particularity in construction. These parts have an effect on construction process. To use unorganic construction information and knowledge properly has become the factor to project success. Therefore, this study analyzes that D-virus of the uneasy factors has an effect on safety control of construction industry. That is to say, aim of the study is to suggest the solution to remove those after confirming for these factors to have certain effect in construction process through fact finding survey and retesting the factors of D-virus to refer in business management in a constructive position.

한국 및 러시아 한국계 청소년 우울증의 사회심리적 위험요인에 대한 횡문화 비교 연구

송동호,민성기,김지웅,김찬형,이홍식,Kim, Larissa 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.5

연구목적 : 본 연구는 사회문화적 배경이 다른 한국과 러시아, 두 나라의 한국인 청소년을 대상으로 우울증의 유병율을 비교 조사하고, 우울증과 관련된 사회심리적 위험요인들을 알아보고자 하였다. 방 법 : 연구도구의 한국어판을 제작하고 두 나라의 청소년 우울증 환자 40명씩을 대상으로 우울척도의 cut-off 점수를 산정하기 위한 예비연구를 본 연구에 앞서 시행하였다. 한국의 청소년 대상은 서울 소재의 중고등학생 838명이었고, 러시아의 청소년은 우즈베키스탄 공화국의 중고등학생 중 양부모가 모두 한국인인 652명이었다. 연구도구로는 우울증의 사회심리적 요인들과 횡문화적 요인들을 조사하기 위하여 기초자료 설문지 및 Beck 우울척도를 적용하였다. 결 과 : 우울증 환자를 대상으로 시행한 예비연구에서 얻은 Beck 우울척도의 cut-off 점수 19점 이상을 기준으로 하였을 때, 한국 청소년 집단에서 우울증의 유병률은 26.5%인데 반하여 러시아 청소년에서는 7.4%의 유병률을 보였다. 한국인 청소년들은 한국인-러시아 청소년에 비하여 염세주의(pessimism), 실패감(failure), 자살사고, 죄책감 등, 절망감(helplessness)과 관련된 증상들이 유의하게 많이 나타났다. 한국인 청소년과 한국인-러시아 청소년 간에 우울증의 유의한 사회심리적 위험요인들로서 여성, 낮은 학업성적, 부모와의 갈등이 공통적으로 나타났으며, 그외에 친구교제 요인, 만성 신체질환, 형제간이나 친구들과의 갈등이 한국인 청소년에서는 또 다른 위험요인으로 존재하였다. 결 론 : 본 연구를 통하여 한국인 청소년의 우울증 유병율이 한국인-러시아 청소년의 유병률에 비하여 유의하게 높았으며, 한국과 러시아의 한국인 청소년들 간에 우울증의 유의한 사회심리적 위험요인들이 차이가 있음을 시사한다. Objectives : In order to study a cross-cultural epidemiology of the adolescent depressives with Korean ethnicity in Korea and Russia, we compared psychosocial characteristics and risk factors of depressive disorder between two school-based populations in different sociocultural environments. Methods : We measured psychosocial variables of 838 subjects in Korea, and 652 in Russia(Uzbekitan) using Basic Questionnaires and Beck Depression Inventory(BDI). Results : The cut-off point of BDI for adolescent depression in Koreans was 19 points. Higher prevalence of depression was noted in Korean adolescents(26.5%) compared to Korean-Russian adolescents(7.4%). We found signicant risk factors of adolescent depression in two study samples : female, academic failure, meeting friends less frequently, presence of chronic physical illness, interpersonal problems with parents, and same-sex and opposite-sex friends. Conclusions : Our data will provide information on cross-cultural understanding of psychosocial characteristics and risk factors in adolescent depression compared between Koreans and Korean-Russians.

醱酵飼料 제조에 관한 硏究 : 3. 醱酵飼料 제조시 水分添加 水準에 따른 아미노산 組成에 관한 硏究 3. A Study on Amino acid Composition as added Moisture Levels

송영민,진상근,갈상완,이성대,김성찬,정맹화 진주산업대학교 농업기술연구소 2001 農業技術硏究所報 Vol.14 No.-

To estimate the essential and nonessential amino acid compositions as added moisture levels in manufacturing fermentation feedstuffs, the three kinds of experimental conditions, T1, T2, T3 were made with rice bran(90kg), leaf mold(10kg) according to added moisture 40kg, 60kg, and 80kg, respectively. In T1 and T2 cases, the contents of threonine, phenylalanine, histidine, aspartic acid, glutamic acid, cystine, arginine increased significantly during fermentation, however, in T3 case, the thereonine content increased significantly after 9 hours fermentation but decreased at 12 hours fermentation(P<0.05). In T1 and T3 cases, the contents of valine, leucine and arginine increased significantly after 9 hours fermentation and decreased at 12 hours fermentation(P<0.05). Methionine increased for three hours fermentation and then did not increase in content in the T1 and the T3. In T2 case, methionine level increased gradually for whole fermentation time. In T3 case, phenylalanine, histidine, aspartic acid, glutamic acid and cystine increased significantly after 9 hours fermentation, but decreased at 12 hours fermentation(P<0.05). In T1, T2 and T3, isoleucine, lysine, serine, proline, glycine, alanine and tyrosine increased significantly during fermentation time(P<0.05).