잎파래 (Enteromorpha linza)의 葉體無菌化와 元形質體 培養

裵光星,宋相鎬,鄭圭和 慶尙大學校 1991 論文集 Vol.30 No.2

잎파래(Enteromorpha linza) 葉體의 無菌化와 葉體에서 分離한 原形質體의 培養에 關한 몇가지 條件을 決定코자 하였다. 洗劑로 葉體의 附着微生物을 除去하기 爲해서는 0.1%液으로 15∼20分間 洗 하는 것이 效果的이었으며, 組織內部의 微生物은 1% KI-I2液으로 2.5∼5分間 處理하여 抗生劑 2%가 添加된 培地함으로서 除去가 可能하였다. 葉體의 原形質體는 2% AAP가 添加된 各種酵素液으로 容易하게 분리할 수 있었다. 原形質體의 無菌培養을 爲한 抗生物質의 添加濃度는 1.5%程道가 適當하였으며 照度는 2000lux以上일 境遇培養에 큰 影響을 미치지는 않는 傾向이었다. Basic studies for obtaining the axenic tissue, isolation and culture of protoplasts of Enteromorpha linza have been attempted. Treating with 0.1% detergent for 15-20mins were effective for cleaning the collected tissue. Exposure the tissue to a 1% KI-I2 solution for 2.5-5.0 mins subsequently culture them in the medium containing 2.0% antibiotic materials were good for inhibition of bacterial growth as well as enzyme solutions composed of abalone acetone powder, cellulase R-10, and driselase. The 2.0% antibiotic medium was good for the suppression of bacterial growth in protoplasts culture. The development of protoplasts was not largely influenced by the light strength and photoperiods.

65세 이상의 노령층에서 고지혈증의 위험요인

김용배,장원기,황보영,김화성,안규동,이병국,이용진,김세훈,김승수,김승훈,김영록,김웅빈,이성수 순천향의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

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연작업자들에서 혈중 및 골중 연량과 조혈기능 지표 사이의 관련성에 ALAD 다형질성이 미치는 영향

김화성,이성수,김용배,황보영,리갑수,안규동,장봉기,이병국 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.1

목 적 : 혈중 연량 및 골중 연량과 조혈기능의 생화학적 지표사이의 관련성에 f-aminolevinicacid dehydratase 효소의 유전형질의 다형질성이 어떠한 영향을 주는지를 알아보고자 연작업자 450명을 연폭로군으로 연폭로가 없는 85명을 대조군으로 선정하였다. 방 법 : 조혈기능의 생화학적 지표로는 혈중ZPP, 요중 ALA, 혈색소 및 혈구용적치를 택하였고, 이들 상호관계에 영향을 줄 수 있는 교란변수로서 성, 연령, BMI, 흡연습관, 음주습관 및 연작업자들의 경우 연폭로 기간을 조사하였다. 결 과 : 동형유전형질인 ALADI을 가진 연작업자들은 407명으로 전체의 90.4 %이었으며, 대조군에서도 77명으로 전체의 90.6 %로서 양군간에 차이가 없었다.동형 유전형질을 가진 연작업자들보다 이형 유전형질을 가진 연작업자들의 골중 연량과 혈중 ZPP의 평균값이 적었으나 통계적으로 유의하지 않았고 나머지 연구변수들의 평균도 유전형질의 구분에 따른차이는 없었다. 성, 직력, BMI, 흡연여부, 음주여부 등의 흔란변수를 통제한 후 혈중 연량 및 골중 연량과 ALAD유전형질이 혈색소에 미치는 영향을 구한 바 혈중 연량과 골중 연량만이 혈색소에 유의한 영향을 주었을뿐 ALAD 유전형질은 유의한 영향이 없었다. 반면에 혈구용적치의 경우에는 혈중 연량과 골중 연량 그리고 유전형질 모두가 유의한 영향을 주지 못했다.요중 ALA를 종속변수로 하고 관련 혼란변수를 통제한 후 혈중 연량과 ALAD 유전형질 그리고 양자의 교호작용을 독립변수로 한 회귀분석들에서 혈중 연량 및 골중 연량과 ALAD 유전형질 모두가 주효과(main effect) 및 교호작용(effect modifies-tion)이 유의하석 이형 ALAD 유전형질을 가진 연작업자들의 ALAU가 동형의 유전형질을 가진 연작업자들보다 상대적으로 낮은 값을 나타냈다.혈중 ZPP를 종속변수로 하고 관련 혼란변수를 통제한 후 혈중 연량과 ALAD 유전형질 그리고 양자의 교호작용을 독립변수로 한 회귀분석들에서 혈중연량 및 골중 연량과 ALAD 유전형질 모두가 음의주효과(maln effect)가 나타나서 이형 유전형질의 연작업자들의 혈중 ZPP가 동형 유전형질의 연작업자보다 상대적으로 적은 값을 나타냈으나 교호작용(effect modification)은 없었다. 결 론 : 상기 결과로 미루어 ALAD 유전형질은 혈색소에는 유의한 영향을 주지 못했으나 ALAU 및 혈중 ZPP에는 유의한 영향을 주어 동형의 유전형질인 연작업자보다 이형의 유전형질 연작업자들이 상대적으로 낮은 ALAU와 혈중 ZPP값을 보여주어이형유전형질이 연에 보호효과가 있다는 가설을 돼받침하였다 Objectives : To evaluate the effect of ALAD polymorphism on the relationship of blood and bone lead with hematologic biomarkers in lead exposed workers, 450 lead exposed workers and 85 non-lead exposed workers were selected. Methods : Blood lead and tibia bone lead were selected as parameters of lead exposure and blood ZPP, urinary ALA (ALAU) , hemoglobin (Hb) and hematocrit (Hct) were chosen as parameters of hematologic effect of lead exposure. Genotype of each subject was assayed and expressed as ALADI and ALAD2. Demographic information such as sex, age and personal habit of smoking and drinking were collected. Job duration of lead exposed workers was also obtained. Results : The overall prevalence of the variant allele, ALAD2 in lead exposed workers was 9.6 %(43 out of 450 lead workers) which was not differed from control workers (9.4 % : 8 out of 85) . The means of tibial lead and blond ZPP in lead workers with ALAD2 were lower than those of lead workers with ALAD1, but the differences were not statistically significant. After adjusting for possible confounders (sex, job duration, BMI, drinking and smoking status) only blood lead and bone lead contributed negatively to the level of hemoglobin with statistical significance without any contribution of ALAD genotype. On the other hand, no significant effect of blood lead, bone lead and ALAD genotype were observed on the level of hematocrit after controlling possible confounder. ALAD genotype in the multiple regression analysis of blood lead fed bone lead with log transformed urinary ALA (LogALAU) after adjusting for possible confounders showed significant main and interaction effect on LogALAU simultaneously, which resulted lower LogALAU in lead exposed workers of ALAD2 than ALAD1. It was also observed only main effect of ALAD gene type on blood ZPP after adjusting possible confounder resulting lower ZPP in lead workers of ALAD2 than ALAD1. Conclusions : With above results, it was found that ALAD polymorphism did not affect on the level of hemoglobin and hematocrit, but ALAD polymorphism made significant effect on the association of blood and bone lead with urinary ALA and blood ZPP. The lower urinary ALA and blood ZPP in ALAD2 lead workers suggested that ALAD2 genotype may be supportive for the protective effect of lead.

언어학습을 위한 전자 사전 관리 시스템 구현

신민화,양호원,배상현 조선대학교 전자정보통신연구소 2002 電子情報通信硏究所論文誌 Vol.5 No.1

Due to the rapid development and growth of users of internet, a lot of remote systems for language studys, Which are the applications of web technology are appearing. For remote systems for language studys, on-line electronic dictionary which allows searching for particular words is on important sector among many methods to enhance the effect studying. In this paper for the manage of language studys to easily many sector and manage the electronic dictionary, we are trying to suggest management system which complement the language studys.

고요산혈증의 위험인자 : 아산시 지역 주민을 대상으로

김용배,장원기,황보영,김화성,안규동,이병국,이용진,이종은,이준숙,이찬구,이한정,이성수 순천향대학교 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

Objective : Serum uric acid has now been identified as a marker for a number of metabolic and hemodynamic abnormalities. The aim of this study is to evaluate risk factors which influence hyperuricemia in the citizens of Asan. Method : The study design was cross-sectional and research subjects were 1086 citizens of Asan. General characteristics, cigarette smoking, alcohol consumption, and past history were obtained by interview using a structural questionnaire. Also, we measured height, weight and blood pressure, and serum uric acid of the subjects. Results : The mean serum uric acid level was 5.27 ㎎/㎗ in men and 4.01 ㎎/㎗, in women, In the multiple logistic regression analysis, statically significant risk factors of hyperuhcemia were gender, body mass index, blood lead concentration, and serum creatinine. Conclusion : Our results suggest that gender, body mass index, blood lead concentration, and serum creatinine might be the most important risk factors of hyperurcemia. Education program as well as routine check-up for serum uric acid was highly recommended for the effective prevention of hyperuricemia.

三天地의 環境과 生物多樣性 : I. Enviornmental Factors and Flora I. 環境要因과 植物相에 關하여

신상천,이종운,배규호,전재인,강경화 嶺南大學校 基礎科學硏究所 1992 基礎科學硏究 Vol.12 No.-

Environmental factors and phytological aspects of Samchunimpoundment in Kyongsan, Kyongpook were studied. 40 quadrats in 4 sites had 94% herbaceous cover, 31% shrub cover and 18% tree cover. Ambrosia artemisiifolia var. elatior, Metaplexis japonica, Erigeron annuus, Humulus Japonicus, Eragrostis ferruginea, Artemisia princeps var. orientalis were dominant herbaceous species and found cultivated plants of Hordeumn vulgare var. hexastichon, Prunus persica, Morus alba and neophytes of Ambrosia artemisiifolia var. elatior, Erigeron canadensis, Erigeron annuus, Ailanthus altissima, Bidens bipinnata, Lactuca indica var. laciniata, Robinia pseudo-acacia, oenothera odorata, Euphobia maculata. The investigated sites were divided into 4 patterns based on the vegetation with high difference level and Ambrosia artemisiifolia var. elatior, Medicago sativa, Setaria viridis, Metaplexis japonica, Humulus japonicus, Calystegia japonica, Forsythia koreana, Lsctuca indica var. laciniata were different species group. More than 90% on the surface area of water mass covered with Nelumbo nucifera, Marsilea quadrifolia and Typha angustata and distributed Hydrilla verticillata, Ceratophyllum demersum, Trypa pseudo-incisa, Myriophyllum spicatum under water. Average data of environmental factor analysis are as followings; air temperature 25.8℃, ph 6.77, total hardness 117.94㎎/ℓ, SS 4.5㎎/ℓ,Do 3.3㎎/ℓ,BOD 6.6㎎/ℓ,COD 7.2㎎/ℓ,cl- 17.56㎎/ℓ,?? 7.14㎎/ℓ,Mn 0.05㎎/ℓ, Zn 0.04㎎/ℓ. Total quality of impoundment water were heavily polluted and were quality class Ⅳ.

메타 전자 쇼핑몰의 설계 및 구현

신민화,김승희,배상현,김판구 조선대학교 전자정보통신연구소 2001 電子情報通信硏究所論文誌 Vol.4 No.1

최근 전자상거래의 상업성이 부각되면서 많은 기업들과 개인사업자들이 독자적인 웹서버를 구축하고 자사의 상품 또는 기업에 대한 광고 내용을 인터넷을 통해 서비스를 하고 있다. 그러나 소비자들에게 인지도가 낮은 기업이나 전국의 각 지방의 특산품과 토산품 관련 웹사이트들은 소비자에게 홍보가 부족하고, 상품의 종류와 정보의 부족으로 외면을 당하고 있는 것을 많이 본다. 본 논문에서는 이러한 기업들의 카탈로그를 수집하여 재구성한 후, 한 사이트에서 여러 기업의 상품을 검색할 수 있도록 하여 소비자의 접근과, 각 기업의 상거래 사이트를 활성화 할 수 있는 메타 전자 쇼핑몰 개발에 관해 기술한다. 본 시스템의 특징은 로봇이 자동으로 데이터 베이스를 구축하여 소비자가 쉽게 검색 할 수 있도록 주제어 검색과 디렉토리 검색 및 폴더형맵 검색 인터페이스 구성을 가지고 있다는 점이다.

한국 재래 우 및 산양 위장관의 somatostatin cell에 대한 면역조직화학적 분포

조성환,김현수,박배근,윤원기,손화영 충남대학교 수의과대학 동물의과학연구소 1995 動物醫科學硏究誌 Vol.3 No.-

The relative frequency and distribution of somatoatatin immunoreactive cells were investigated by the immunohistochemical method in the gastrointestinal tract of the Korean native cattle and goat. The results obtained were as follows: In the Korean native cattle, somatoststin cells were a few in the pyloric and duodenal region and rarely seen in fundic region, jejunum, ileum, cecum, proximal colon, central coil, distal colon and rectum. In the Korean native goat, somatostatin cells were the most numerous in the pyloric region and the next in the fundic region. And they were a few in the duodenum, not detected in the cecum and sparse in the jejunum, ileum, proximal colon, central coil, distal colon and rectum.

태권도 수련복의 변천과정에 관한 고찰

윤상화,권관배 龍仁大學校 1998 용인대학교 논문집 Vol.16 No.-

The purpose of this inquiry is to understand the changing process of Tae-Kwon Do uniform by After thorough examination, they could be summarized as below. Tae-Kwon Do training uniforms are divided into two different types. One is a spread extended type which originally came form Japan and the other is an Korean traditional martial arts uniform. As the development of Tae-Kwon Do occurred, the uniform changed into a basic clothing form from the three countries period which included a black collar and a half black and red collar for grade holders. The differences in the length of sleeves and pants come from which material it is made of and a statement regulated by the Korea Tae-Kwon Do Federation. The uniform currently using is not the Korean traditional type which has to be worn over the head. This has a major disadvantage when putting it on and taking it off, it ruins the hair especially after sweating a lot through training. As a result, the uniform we are currently wearing has a slight problem in practicality and the historical background but the uniform itself is rogarded more as a sport uniform rather than a training uniform.

개에서 관찰된 모기질세포종의 증례보고

조성환,손화영,김혜성,최종윤,류시윤,박배근,윤원기,홍성혁,김덕환 충남대학교 수의과대학 동물의과학연구소 2002 動物醫科學硏究誌 Vol.10 No.-

Pilomatricoma was histopathologically diagnosed in a 2.5-year-old male Alaskan malamute. The patient was referred to Chungnam National University, Veterinary Medical Teaching Hospital with solitary, firm and well-circumscribed mass at shoulder. Grossly, the mass was dome shaped to tumor like and 10 x 8 x 5㎝ in size. Some lesions had gritty or bony consistency and was chalky when sectioned Histopathologic feature included mumerous clusters of tightly arranged, basaloid cells with evenly distributed chromatin and ghost cells were characterized by a central unstained zone. The ghost cells often become calcified and osseous metaplasia was also present.