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Lee, Moon Hee,Cha, Hee-Jae,Choi, Eun Ok,Han, Min Ho,Kim, Sung Ok,Kim, Gi-Young,Hong, Su Hyun,Park, Cheol,Moon, Sung-Kwon,Jeong, Soon-Jeong,Jeong, Moon-Jin,Kim, Wun-Jae,Choi, Yung Hyun Spandidos Publications 2017 International journal of molecular medicine Vol.39 No.3
<P>Natural phytochemicals of plant origin, including flavonoids, have been found to be potent antioxidants providing beneficial effects against oxidative stress-related diseases. The present study was carried out to investigate the antioxidant properties of morin, a flavonoid originally isolated from the flowering plants of the Moraceae family. Superoxide dismutase (SOD)-like activity and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(center dot+)) radical scavenging activity were determined. We also investigated the cytoprotective effects of morin against hydrogen peroxide (H2O2)-induced DNA damage and apoptosis in V79-4 Chinese hamster lung fibroblasts. Our results demonstrated that morin had strong scavenging effects against ABTS' radicals with enhanced SOD activity, which varied in a dose-dependent manner. Morin was found to reduce H2O2-induced intracellular reactive oxygen species generation and nuclear DNA damage, and it recovered cell viability damaged by H2O2 via inhibition of mitochondrial dysfunction-mediated apoptosis. Notably, the treatment of V79-4 cells with morin markedly enhanced the expression of heme oxygenase-1 (HO-1) but not quinone oxidoreductase-1, which was associated with the increased expression and phosphorylation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and the downregulation of Kelch-like ECH-associated protein 1 expression. Based on our findings, we conclude that morin effectively ameliorated oxidative stress-induced DNA damage through intrinsic free radical scavenging activity and activation of the Nrf2/HO-1 pathway.</P>
Lee, Moon Hee,Han, Min Ho,Lee, Dae-Sung,Park, Cheol,Hong, Su-Hyun,Kim, Gi-Young,Hong, Sang Hoon,Song, Kyoung Seob,Choi, Il-Whan,Cha, Hee-Jae,Choi, Yung Hyun UNKNOWN 2017 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.39 No.2
<P>In the present study, we investigated the cytoprotective efficacy of morin, a natural flavonoid, against oxidative stress and elucidated the underlying mechanisms in C2C12 myoblasts. Our results indicated that morin treatment prior to hydrogen peroxide (H2O2) exposure significantly increased cell viability and prevented the generation of reactive oxygen species. H2O2-induced comet-like DNA formation and gamma H2AX phosphorylation were also markedly suppressed by morin with a parallel inhibition of apoptosis in C2C12 myoblasts, suggesting that morin prevented H2O2-induced cellular DNA damage. Furthermore, morin markedly enhanced the expression of heme oxygenase-1 (HO-1) associated with the induction and phosphorylation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and the inhibition of Kelch-like ECH-associated protein 1 (Keapl) expression. Notably, these events were eliminated by transient transfection with Nrf2-specific small interfering RNA. Additional experiments demonstrated that the activation of the Nrf2/HO-1 pathway by morin was mediated by the extracellular signal-regulated kinase (ERK) signaling cascade. This phenomenon was confirmed with suppressed Nrf2 phosphorylation and consequently diminished HO-1 expression in cells treated with a pharmacological inhibitor of ERK. Collectively, these results demonstrated that morin augments the cellular antioxidant defense capacity through the activation of Nrf2/HO-1 signaling, which involves the activation of the ERK pathway, thereby protecting C2C12 myoblasts from H2O2,-induced oxidative cytotoxicity.</P>
서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)
김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2
Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.
차상민,배희은,최진호,최영휴 국립7개대학공동논문집간행위원회 2003 공업기술연구 Vol.3 No.-
During high-speed running of machine tools, serious vibrations often may occur in their feed-drive system. In this paper, a 4-D.O.F. planar motion model is proposed in order to analyze a ball screw feed-drive system. The equations of motion are derived for the proposed model and natural frequencies of the system are calculated. Also its transient response due to driving motor speed control input are calculated. The theoretical analysis results showed good agreement with experimental ones measured from a laboratory feed-drive test system. Consequently we concluded that the proposed 4-D.O.F model can be well applicable to the vibration analysis and dynamic design of machine tool feed-drive system.
공초점 레이저 주사 현미경을 이용한 법랑질 초기 우식 재광화의 정량적 분석
차승우,윤태철,박성호,이찬영,금기연 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.1
Dental Caries which has high prevalence rate, accounts for majority of dental diseases. Many treatment and preventive treatment has been developed, thereby reducing the prevalence rate, but in our country, flouridization has not spread widely yet, so prevention has not been done satisfactorily. When dental caries progresses, irreversible damage of tooth structure occurs. In initial dental caries. Demiteralized tooth structure can be remineralized, so restorative treatment is unnecessary. In the study, 20 teeth restored with composite resin without fluoride release were used and divided into two groups. Incipient dental caries were artificially made and remineralization procedure was done for 1 and 2 weeks, for each group. Changes in mineral contents aroud the margins were analysed with confocal laser scanning microscope. The results were as follow. 1.Both total fluorescence of the lesion and average fluorescence of the lesion of remineralized samples decreased compared to demineralized state. (p<0.01) 2.Confocal laser scanning microscopy can be used in quantitative analysis of mineral change. In result, confocal laser scanning microscopy can be used in quantitative analysis of mineral change and it could be used in many different fields of dentistry in the future. Key words : Confocal laser scanning microscopy. Demineralization. Remineralization, Initial dental caries
Chloramphenicol이 Mouse 십이지장 상피조직의 Alkaline Phosphatase 및 ??, ?? 활성에 미치는 영향
차상훈,이군자,이규식,정호삼 漢陽大學校 環境科學硏究所 1991 環境科學論文集 Vol.12 No.-
체중 20gm 내외의 ICR계 웅성 mouse에 체중 ㎏당 200㎎의 chloramphenicol을 매일 복강내로 주사한 후 희생전 12시간 동안 절식시키고 투여 제 5 일 및 제10일에 각각 희생시켜 십이지장을 적출하고 alkaline phosphatase 활성 및 ??, K?? 활성을 관찰하여 다음과 같은 결과를 얻었다. 1. Alkaline phosphatase 활성은 대조군 mouse 십이지장의 상피세포층에서는 강한 양성반응을 나타내었으나 chloramphenicol 5일 투여군에서는 중등도의 양성반응을 나타내었고 chloramphenicol 10일 투여군에서는 약한 양성반응을 나타내었다. 2. ??, ?? 활성은 대조군 mouse 십이지장의 상피세포층에서는 강한 양성반응을 나타내었으나 chloramphenicol 5일 투여군 및 chloramphenicol 10일 투여군에서는 모두 중등도의 양성반응을 나타내었다. 이상의 소견을 족합하면 chloramphenicol은 십이지장 상피세포에 손상을 야기시켜 상피세포의 기능을 저해하는 것으로 사료된다. Albino mice, ICR strain, weighing 20gm were used as experimental animals and chloramphenicol(20㎎/㎏) was administered to the experimental animals once a day for 10 days. The experimental animals were fasted 12 hours before sacrifice and ascrificed at 5 days and 10days after initial drug administration. For the evaluation of activities of ??, ?? and alkaline phosphatase the duodenal specimens were stained by Guth and Albert's method and Gomori's method, respectively. The results were as follows. 1. The activity of alkaline phosphatase in the epithelial layer of control mouse was strongly positive, while that of 5 days chloramphenicol treated mouse was moderately positive and that of 10 days chloramphenicol treated mouse was weakly positive. 2. The activity of ??, ?? in the epithelial layer of control mouse was strongly positive, while those of 5 days and 10 days chloramphenicol treated mouse was moderately positive. Consequently, it is suggested that chloramphenicol induce damages in the duodenal epithelium, so that it inhibits the function of the duodenal epithelium.