子宮摘出이 家兎 卵巢의 機能에 미치는 影響에 關한 實驗的 硏究

金善行,羅重烈,李壽鍾 고려대학교 의과대학 1983 고려대 의대 잡지 Vol.20 No.3

To understand effects of hysterectomy on the retained ovarian function in the rabbits, pseudopregnancy was induced in seven rabbits, control group, by injection of 50㎍ of ethinyl estradiol and 100 IU of hCG subcutaneously. And a week after hysterectomy of another seven rabbits, hysterectomized group, pseudopregnancy was induced with 50㎍ of ethinyl estradiol and 100 IU of hCG. The serum levels of progesterone and estrogen were determined every 3 day by radioimmunoassay and microscopic examination of the ovaries was performed in each group. Both findings in each group was compared and obtained following results. 1. Luteal phase in hysterectomized group was prolonged about 6 days compared to control group. 2. Histologic examination of ovaries revealed functional corpora lutea in hysterectomized group up to 30 days of experiment. 3. Serum estradiol level and histological picture of the ovarian follicles showed no significant differences between two groups during experiment. 4. These findings show that experimental hysterectomy has inhibitory effect on regressive change of the corpora lutea but has no effect on ovarian follicle. These results suggest that PG is the luteolytic hormone in the rabbit. But, it was presumely concluded that the result in estrogen showed which PGF₂α or some other substances in the uterus might be related to the mechanism of folliculogenesis in ovary according to references.

다래나무 및 노각나무 수액의 성분조성

최선영,나성택,김용환,김행자,성낙주 경상대학교 농업생명과학연구원 2002 농업생명과학연구 Vol.36 No.2

본 실험에서는 다래나무 및 노각나무 수액을 천연 건강음료로 개발하기 위한 기초 자료를 얻고져 물리ㆍ화학적 성질과 유리당, 무기물 및 구성 아미노산 등을 분석하였다. 다래나무와 노각나무 수액의 pH, 비중, 당도, 수분, 회분 및 조단백질의 함량은 거의 비슷하였다. 유리당 함량은 fructose가 다래나무 수액에서 378.4㎎/100㎖, glucose는 노각나무 수액에서 125.4㎎/100㎖로 가장 높은 함량이었다. 무기물 조성은 망간과 알루미늄을 제외한 모든 무기물에서 노각나무 수액에 비해 다래나무 수액에서 함량이 높았으며 특히 칼슘은 다래나무 수액이 172.0㎎/ℓ, 노각나무 수액에서 13.7㎎/ℓ로 다래나무 수액이 노각나무 수액보다 약 13배나 높은 함량을 보였다. 다래나무 및 노각나무 수액은 분석된 총 17종의 구성 아미노산 중 다래나무 수액은 4종, 노각나무 수액에서는 6종이 검출되었는데, 다래나무 수액은 cystine, aspartic acid, serine 그리고 valine의 순으로 노각나무 수액에서는 cystine, glutamic acid, aspartic acid, serine, valine 및 phenylalanine 등의 순으로 함량이 높게 나타났다. Natural compounds such as free sugars, minerals and composition amino acids in Darae(Actinidia arguta) and Korean stewartia(Stewartia koreana) saps were analyzed to establish to the basic data of foods. Free sugars of the saps determined were fructose and glucose which were detected higher amounts as 378.4 and 125.4㎎/100㎖, respectively, but sucrose and maltose were not detected. Among 10 kinds of mineral detected in the saps, the content of calcium in Darae and aluminum in the Korean stewartia saps was the highest 172.0 and 56.1㎎/ℓ, respectively. The calcium content in Darae saps was 13 times higher than that of Korean stewartia saps. The Darae saps were composed of 4 kinds of amino acids such as cystine, aspartic acid, serine and valine, and then Korean stewartia saps 6 kinds of amino acids such as cystine, glutamic acid, aspartic acid, serine, valine and phenylalanine. It is concluded that saps of Darae and Korean stewartia contain a good variety of natural compounds such as free sugars, minerals and amino acids to serve as an excellent source of very natural and health promoting drinks.

녹차 및 매실 추출물이 N-Nitrosodimethylamine의 생성에 미치는 영향

최선영,정미자,신정혜,김행자,성낙주 한국식품영양학회 2002 韓國食品營養學會誌 Vol.15 No.1

녹차 및 매실추출물이 아질산염 소거, 전자 공여능 및 NDMA 생성 억제에 미치는 영향을 분석하고, 질산염과 아민이 풍부한 식단(CW)에 인공타액과 위액을 이용한 "simulated digestion" 에서 확인하였다. 녹차와 매실 추출물의 아질산염 소거작용을 pH 1.0, 4.2 및 6.0에서 실험 결과, pH가 낮을수록, 시료 첨가량이 많을수록 효과적이었으며 pH 1.2에서 녹차 추출물 0.5ml 첨가시킬 경우 99.6%의 높은 소거작용을 나타내었고, 매실 추출물 3ml 첨가시 77.2%의 소거작용을 나타내었다. 녹차 및 매실 추출물의 전자공여 작용은 각각 70.6%, 75.1%로 높게 나타났다. 녹차와 매실 추출물의 NDMA 생성억제 효과는 pH 1.2 에서 가장 뛰어났고, 시료량이 증가할수록 억제효과가 높아 3ml 첨가시에 82.1%와 73.2%의 억제효과를 나타내었다. 실험식이에 녹차 및 매실 추출물의 첨가량을 달리하여 인공소화시킬 때 NDMA 생성억제 효과는 시료 첨가량에 비례하여 각각 20ml 첨가시 녹차 추출물은 57.9%, 매실 추출물은 48.4%의 NDMA 생성억제 효과를 나타내었다. The effects of nitrite scavenging, electron donating and N-nitrosodimethylamine (NDMA) formation in vitro and green tea (Camellia sinensis) and Maesil (prunus mume) were studied. The green tea and Maesil extracts were tested for their nitrite-scavenging effect under the different pH conditions such as pH 1.2, 4.2 and 6.0. The effects of nitrite-scavenging in all concentrations were diminished in the alkali condition, whiled its effects in the acidic condition of pH 1.2 were reached of more than 99.0% by adding above 0.5ml of green tea extract. And also, nitrite-scavenging effect by adding 3ml of Maesil extract was about 77.0%. The electron donating ability (EDA) of green tea and Maesil extracts was 70.6%, 75.1%, respectively. The formation of NDMA was very effectiveness which was inhibited 82.1%, 73.2% at reaction mixture of pH 2.5 adding 3ml of above extracts, respectively. The ground CW, TW1 and TW2 (refer to Table 1) diets were incubated with 10ml simulated saliva and 40 ml gastric juice at 37℃ for 2hrs. NDMA formation was inhibited at all levels of green tea and Measil extracts.

韓國人 妊産婦 및 新生兒 血淸 Immunoglobulins에 關한 硏究

申載昇,金善行,洪性鳳 고려대학교 의과대학 1985 고려대 의대 잡지 Vol.22 No.1

Levels of the three major classic immunoglobulins(IgG, IgA, IgM) were measured by rad a1 immunodiffusion plate method in paired maternal and cord sera at the time of delivery. Primarily, the purpose of this study is to establish the baseline of serum immunoglobulin levels in the normal term pregnant women and their fetuses in Korea, and to evaluate the correlations between serum immunoglobulin levels and various factors which were proposed that can influence the serum concentrations of immunoglobulins. The values obtained in maternal and fetal sera were submitted to statistical analysis to know the correlations with delivery method, parity, fetal body weight and monthly expenditure. 1. The levels of IgG in maternal and cord sera were 1129±15.9mg per 100m1 and 1245±25.2mg per 100ml. 2. The levels of IgA in maternal and cord sera were 225±7.1mg per l00m1 and 27±4.2 mg per 100ml. 3. The levels of IgM in maternal and cord sera were 155±3.5mg per l00m1 and 4o±2.1 mg per 100ml. 4. There was no close correlation between the immunoglobulin levels in the maternal sera and the monthly body weight. 5. There was no close correlation between the immunoglobulin levels in the maternal sera and the monthly expenditure. 6. There was no significant difference in maternal and fetal cord sera immunoglobulins levels between normal vaginal delivery group and elective cesarean section group. 7. There was no significant difference in maternal and fetal cord sera immunoglobulins levels between primiparous group and multiparous group. 8. There was siginificant correlation between the IgM levels of mother and the fetus, but not in IgG and IgA.

정신분열증 환자의 신체증상과 인구통계학적, 임상인자들과의 관련성

윤정흠,김선경,전현태,이귀행 원광대학교 의과학연구소 2009 圓光醫科學 Vol.24 No.1

배경 : 이 연구의 목적은 정신분열병 환자의 신체증상과 인구통계학적, 임상인자들과의 관련성을 알아보기 위함이다. 방법 : 연구 대상군은 입원중인 63명의 정신분열병 환자를 대상으로 하였다. 신체 증상의 정도는 한국어판 신체증상목록을 사용하여 측정하였다. SPSS 11.5를 이용하여 신체증상의 정도와 우울, 불안, 교육정도, 적응상태와의 관계를 피어슨 상관분석을 통하여 평가하였다. 결과 : 1) 신체증상의 정도는 교육기간과 입원 당시의 적응 상태와는 부적 상관을 보였다(p<0.05). 2) 신체증상의 정도는 우울, 불안과는 정적 상관을 보였다(p<0.05). 3) 정신분열병 환자의 신체 증상의 빈도는 다음 순이었다: 두통, 피로, 수면장애, 건강염려, 현기증, 오심, 위장장애, 근력 약화, 허리나 등의 통증, 복부의 가스. 결론 : 정신분열병의 신체증상의 정도는 교육기간이 길고 입원 당시의 적응상태가 좋을수록 감소하였다. 그리고 신체 증상은 우울, 불안의 증상이 심할수록 증가하였고, 이는 특히 신체증상이 우울, 불안과 관련이 있다는 것을 알 수 있었다. Background : The purpose of this study was to examine the correlation between somatic symptoms and demographic and clinical variables in schizophrenia patients. Methods : The subject were 63 schizophrenia patients ; male(n=35, mean age =39), female(n=28, mean=age 40). The degree of somatic symptoms was measured using the Korean versions of Wahler Physical Symptom Inventory(WPSI). The relationship among somatic symptoms, anxiety, depression, education period and adjustment was evaluated by Pearson correlation with SPSS 11.5. Results : 1) The degree of somatic symptoms showed negative correlation with education period and current adjustment at admission(p<0.05). 2) The degree of somatic symptoms was significantly correlated with degree of the depression and the anxiety(p<0.05). 3) The frequent somatic symptoms were as follows in order : headaches, feeling tired, difficulty sleeping, poor health in general, dizzy spells, nausea, intestinal or stomach trouble, muscular weakness, backaches and excessive gas. Conclusion : The somatic symptoms of schizophrenia were decreased in the group of the long education period and the good current adjustment at admission. The more depression and anxiety were increased, the more somatic symptoms were revealed. It suggests that somatic symptoms of schizophrenia may be related to depression and anxiety.

이주 노동자들의 생물학적 노출 지표와 건강 실태 : 2005년 특수건강진단 결과 자료 토대

송윤희,김규상,이선웅,최선행 大韓産業醫學會 2008 대한직업환경의학회지 Vol.20 No.2

목적: 이 연구의 목적은 2005년 특수건강진단 자료를 이용하여 이주 노동자들의 유해 요인 노출 실태와 일반 적인 건강 실태를 국내 노동자와 비교하여 파악하고자 하였다. 방법: 2005년 특수건강진단 자료 중 이주 노동자 25,086명의 자료와 같은 해 경인지역 3개 기관에서 특수 건강진단을 시행한 국내 노동자 19,616명의 자료를 이용하여,생물학적 노출지표와 몇 가지 주요 임상 검사 결과를 국내 노동자와 이주노동자로 나누어 비교 분석하였다. 동칠한 표본의 비교를 위해 이주 노동자 자료에서 경인 지역의 동일 3개 기관의 결과만을 선발하여 비교하고(국내 19,616명,이주 1,886명), 전체 대상으로 확장하여서 비교하였으며 (국내 19,616명,이주 19.616명), 마지막으로 동일 업종, 3년 미만 근로기간, 50인 미만 규모로 제한하여 비교하였다(국내 2,910명,이주 노동자10,022명) 임상 검사는 3개 기관의 20대와 30대만을 선발하여 비교하였다. 결과: 동질 표본의 비교에서 혈중 납은 국내 6.09 μg/dl. 이주 8.37μg/L로 혈중 카드뮴은 국내 0.29 μg/dl, 이주 0.36μg/L로 나와 국내 노동자에 비해 이주 노동자들이 더 높게 나왔다. 하지만 유기용제의 생물학적 노출지표는 반대로 국내 노동자에게서 더 높게 나왔다. 주요 임상 검사 결과로는 간 수치 중 AST/ ALT가 국내 8.1/6.8%, 이주 5.7/5.4%로 국내 노동자들이 더 간 건강이 좋지 않은 것으로 나왔고 빈혈은 국내 9.0%,이주11.0%로 이주 노동자에게서 그 유병율이 더 높게 나왔다. 결론: 이주 노동자들이 국내 노동자들에 비해서 중금속에 한한 더 열악한 작업환경에 근무하고 있다는 것을 유추해 볼 수 있었다. 하지만 유기용제의 노출 지표에 대해서는 명확한 해석을 하기 어려웠으며 이에 대해서는 추후 더욱 체계화된 연구가 필요할 것으로 판단된다. Objectives: To investigate the general health status and seventy of exposure to hazardous agents - with a focus on heavy metals - of migrant workers utilizing the 2005 Special Health Examination data. Methods: With data from the 2005 Special Health Examination, we examined the biologic exposure indices and several major clinical exam items of 25,086 migrant workers in the whole country in comparison with those of 19,616 native Korean workers in Kyunggi-do province. Of these we chose homogeneous samples from the same 3 health service centers in Kyunggi-do. (native workers: 19,616, migrant workers: 1,886)to be more precise. Results: The results from the samples of the 3 centers were as follows. Blood lead (natives: 6.09 μg/dl, migrants: 8.37 μg/L) and cadmium (natives: 0.29 μg/dl, migrants: 0.36 μg/L) were higher in the migrant workers than in the native Korean workers, whereas the biologic exposure indices of organic solvents were higher in the native workers. As for major clinical exam items, the liver battery was worse in the natives with incidence of abnormal AST/ALT level (natives: 8.1/6.8% migrants: 5.7/5.4%, p-va1ue<0.01 for AST). Anemia was more prevalent in the migrant workers (natives: 9.0% migrants: 11.0%, p-va1ue<0.05). Conclusions: Migrant workers in Korea are more frequently or more severely exposed to a hazardous working environment containing heavy metals. However, we were not able to explain the results for the exposure indices or organic solvents, and such an explanation will require further study in the future.

대혈관 전위증의 병리

조수연,오미혜,김정선,이용희,강행지,서정욱 한국소아심장연구회 2003 소아심장 Vol.7 No.2

정신분열병 환자의 인지기능 상태

이지혜,오소영,윤정흠,김선경,이귀행 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.1

목적: 정신분열병 환자의 적응 상태와 증상 형성에 영향을 주는 것으로 생각되는 인지기능 상태를 주의력, 형태 인식, 단어에 대한 구별과 종합 기능을 평가해봄으로써 알아보고자 하였다 방법: 정신분열병 환자 27명과 대조군 27명을 대상으로 하였고, 평가 도구로써 주의력은 K-WAIS의 숫자외우기 문제로, 형태 인식은 K-WAIS의 모양 맞추기 문제와 숨은 그림 찾기로, 단어 기능은 K-WAIS의 공통성 문제와 단어 차이 검사로 각각 이루어졌다. 통계 처리는 SPSS 11.5를 이용하여 Chi-Square test와 Student's t-test로 검증을 하였다. 결과: 정신분열군은 숫자외우기, 숨은 그림 찾기, 모양 맞추기에서는 대조군보다 낮은 점수를 보였다. 단어 차이 검사와 공통성 문제에서는 양군에서 차이를 보이지 않았다. 결론: 본 연구에서 정신분열군의 인지 기능은 정상인 대조군에 비해 주의력과 형태 인식 기능이 유의하게 떨어져 있었으며, 단어들을 구분하고 종합하는 능력은 차이가 없었다. 이런 결과는 임상적으로 정신분열병에서 보이는 망상이 언어적인 측면에서는 어느 정도 전달이 되는 현상과 일치하지만 주의나 형태인지와 같은 기본적인 과정에서 장애가 있다는 추정을 하게 한다. Purpose: This study aimed to investigate the cognitive function in patients with schizophrenia by assessing attention, recognition of a shape, and verbal function. Methods: The subjects were 27 patients with schizophrenia group and 27 control group. Attention was assessed by the digit span task(K-WAIS subtest). The recognition of a shape was assessed by the object assembly task(K-WAIS subtest) and hidden figure task. Verbal function was assessed by Similiarities(K-WAIS subtest) and the different word task. Analysis was performed by the Chi-Square test and Student's t-test via SPSS 11.5. Result: Schizophrenia group had statistically lower scores than control group in digital span, hidden figure and object assembly. There was no difference between two groups in dimensions of difference in words and similarity. Conclusion: This study showed that patients with schizophrenia had impairments in attention and recognition of shape, and no difference between two groups in verbal ability. These explained the partial communicative function of delusion in verbal level. The formation of delusion might be related to the more basic elements such as attention and recognition of shape.

Primary serous papillary peritoneal carcinoma arising from supernumerary ovary in mayer-rokitansky-kuster-hauser syndrome (mrkh) (초)

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HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer

Kim, Sun-Yong,Kim, Hyo Jeong,Kim, Haeng-Jun,Kim, Dae Ho,Han, Jae Ho,Byeon, Hyung Kwon,Lee, Keunho,Kim, Chul-Ho Informa UK (TaylorFrancis) 2018 AUTOPHAGY Vol.14 No.3

<P>HSPA5/GRP78/BiP plays an important role in cell survival or tumor progression. For these reasons, HSPA5 is an emerging therapeutic target in cancer development. Here we report that HSPA5 contributes to head and neck cancer (HNC) survival via maintenance of lysosomal activity; however, a nonthermal plasma (NTP, considered as a next-generation cancer therapy)-treated solution (NTS) inhibits HNC progression through HSPA5-dependent alteration of lysosomal activity. HSPA5 prevents NTS-induced lysosome inhibition through lysosomal-related proteins or regulation of gene expression. However, NTS-induced MUL1/MULAN/GIDE/MAPL (mitochondrial ubiquitin ligase activator of NFKB 1) leads to downregulation of HSPA5 via K48-linked ubiquitination at the lysine 446 (K446) residue. MUL1 knockdown hinders NTS-induced lysosome inhibition or cytotoxicity through the reduction of HSPA5 ubiquitination in HNC cells. While MUL1 was suppressed, HSPA5 was overexpressed in tissues of HNC patients. NTS strongly inhibited HNC progression via alterations of expression of MUL1 and HSPA5, in vivo in a xenograft model. However, NTS did not induce inhibition of tumor progression or HSPA5 reduction in MUL1 knockout (KO) HNC cells which were generated by CRISPR/Cas9 system. The data provide compelling evidence to support the idea that the regulation of the MUL1-HSPA5 axis can be a novel strategy for the treatment of HNC.</P>