Contrast Volume/Raw eGFR Ratio for Predicting Contrast-Induced Acute Kidney Injury in Patients Undergoing Percutaneous Coronary Intervention for Myocardial Infarction

Park, Hoon Suk,Kim, Chan Joon,Yi, Jeong-Eun,Hwang, Byung-Hee,Kim, Tae-Hoon,Koh, Yoon Seok,Park, Hun-Jun,Her, Sung-Ho,Jang, Sung Won,Park, Chul-Soo,Lee, Jong Min,Kim, Hee Yeol,Jeon, Doo Soo,Kim, Pum-Jo S. Karger AG 2015 Cardiorenal medicine Vol.5 No.1

<P>Abstract</P><P><B><I>Background:</I></B> Considering that contrast medium is excreted through the whole kidney in a similar manner to drug excretion, the use of raw estimated glomerular filtration rate (eGFR) rather than body surface area (BSA)-normalized eGFR is thought to be more appropriate for evaluating the risk of contrast-induced acute kidney injury (CI-AKI). <B><I>Methods:</I></B> This study included 2,189 myocardial infarction patients treated with percutaneous coronary intervention. Logistic regression analysis was performed to identify the independent risk factors. We used receiver-operating characteristic (ROC) curves to compare the ratios of contrast volume (CV) to eGFR with and without BSA normalization in predicting CI-AKI. <B><I>Results:</I></B> The area under the curve (AUC) of the ROC curve for the model including all the significant variables such as diabetes mellitus, left ventricular ejection fraction, preprocedural glucose, and the CV/raw modification of diet in renal disease (MDRD) eGFR ratio was 0.768 [95% confidence interval (CI), 0.720-0.816; p < 0.001]. When the CV/raw MDRD eGFR ratio was used as a single risk value, the AUC of the ROC curve was 0.650 (95% CI, 0.590-0.711; p < 0.001). When the CV/MDRD eGFR ratio with BSA normalization ratio was used, the AUC of the ROC curve further decreased to 0.635 (95% CI, 0.574-0.696; p < 0.001). The difference between the two AUCs was significant (p = 0.002). <B><I>Conclusions:</I></B> Raw eGFR is a better predictor for CI-AKI than BSA-normalized eGFR.</P><P>© 2015 S. Karger AG, Basel</P>

류마티스 관절염 환자에서 혈장 호모시스테인, 혈청 비타민 B12, 엽산 농도에 관한 연구

차훈석 ( Hoon Suk Cha ),전찬홍 ( Chan Hong Jeon ),고재현 ( Jay Hyun Koh ),이창근 ( Chang Keun Lee ),김진석 ( Jin Seok Kim ),김종원 ( Jong Won Kim ),강성욱 ( Seong Wook Kang ),송영욱 ( Yeong Wook Song ),고은미 ( Eun Mi Koh ) 대한류마티스학회 1999 대한류마티스학회지 Vol.6 No.3

Objective: Cardiovascular disease is the most frequent cause of death in rheumatoid arthritis(RA) patients and hyperhomocysteinemia is an independent risk factor for cardiovascular disease. We evaluated the status of homocysteine, vitamin B12 and folate in RA patients and the influence of the drugs used in RA on homocysteine and vitamin levels Methods: Fifty-six RA patients and 22 controls were studied. Plasma total homocysteine was measured by HPLC method and serum folate and vitamin B12 were measured by chemiluminescence immunoassay. In RA patients, age, sex, disease duration, medications and laboratory findings were analyzed. Results: Serum vitamin B12 level was significantly lower in RA patients compared to controls(p=0.033). No significant difference in serum folate level was found between RA patients and controls but plasma total homocysteine level was significantly higher in RA patients. There was no difference in plasma total homocysteine level between patients taking MTX with folate and controls, but plasma total homocysteine level was significantly higher in patients not taking MTX compared with controls(p=0.028). In RA patients taking only hydroxyl-chloroquine(HCQ) as a DMARD, there was significantly lower serum folate level(p=0.033) and higher plasma total homocysteine level(p=0.043) compared with controls. There was a significant negative correlation between plasma total homocysteine level and serum folate level in RA patients(r=-0.319, p=0.017). Conclusion: Plasma total homocysteine level was increased in RA patients but not in patients taking MTX and folate. These findings suggested that folate supplementation may be effective to prevent hyperhomocysteinemia in RA patients.

Genome Mapping of an Extreme Thermophile, Thermus caldophilus GK24

Park, Jong Hoon,Park, Byung Chul,Koch, Suk Hoon,Kim, Joong Soo,Koh, Jeong Heon,Yang, Moon Hee,Kim, Yong Sung,Kim, Cheorl Ho,Kim, Myoung Hee,Kwon, Suk Tae,Lee, Dae-Sil Korea Genome Organization 2003 Genomics & informatics Vol.1 No.1

Genome of an extreme thermophile, Thermus caldophilus GK24 has been analyzed to construct the genomic map. The genomic DNAs encapsulated in agarose gel were digested with SspI, EcoRI, SpeI, and HpaI restriction endonucleases, and then the resulting genomic DNA fragments were analyzed by pulsed-field gel electrophoresis. Its restriction map has been constructed by analyzing sizes of the restriction fragments obtained from both complete and partial digestions. The circular form of its genome was composed of about 1.98 Mbp and a megaplasmid. The genomic loci for the genes of xylose isomerase, thioredoxin, tRNA-16S rRNA, 23S rRNA, L5 ribosomal protein, ADP-glucose pyrophosphorylase, DNA-ligase, and Tca DNA polymerase were determined by both Southern hybridization and PCR.

지능형 전력관리시스템의 구축사례

고강훈(Kang-Hoon Koh),김천식(Chun-Sik Kim),김수용(Soo-Young Kim),박정우(Jung-Woo Park),고희석(Hee-Suk Koh),이현우(Hyun-Woo Lee) 한국조명·전기설비학회 2000 한국조명·전기설비학회 학술대회논문집 Vol.2000 No.-

This paper is a study if remote control system with KEN that is not only superior-manufacture ability-hardware; that is to say, the hardware is HMI/SCADA solution and it has been developed to sub-station and power management system, which is easy, fast and suitable for users to work with, but also compatible solution with domestic environment. Futhermore, the hardware has been developed by completely domestic technology with knowledge from various actual experiences. In the long run, for uninterruptible power supply to train, building, and factory, real time tele-supervisory control to power facilities.

미만성 폐포출혈을 동반한 전신성 홍반성 루푸스 환자에서 Plasmapheresis를 이용한 치험

고재현 ( Jay Hyun Koh ),송서영 ( Seo Young Song ),이창근 ( Chang Keun Lee ),서기현 ( Gi Hyeon Seo ),안홍준 ( Hong Joon Ahn ),차훈석 ( Hoon Suk Cha ),김진석 ( Jin Seok Kim ),고은미 ( Eun Mi Koh ),송재훈 ( Jae Hoon Song ) 대한류마티스학회 1999 대한류마티스학회지 Vol.6 No.2

Pulmonary alveolar hemorrhage (PAH) is a rare and often fatal presenting feature of systemic lupus erythematosus (SLE) and enters the differential diagnosis of diffuse lung disease in patients with SLE. Reported mortality rates are extremely high, between 70 and 90 percents. Because death frequently occurs within the first several days of the hemorrhage, the diagnosis needs to be established promptly and treatment should be initiated immediately. Treatment of alveolar hemorrhage has included various combinations of corticosteroids, cytotoxic agents, and plasmapheresis, but survival rates have been extremely low despite aggressive therapy. We experienced a case of diffuse alveolar hemorrhage in a 29 year-old SLE male patient. PAH was diagnosed by hemoptysis, anemia, infiltration on chest X-ray and hemosiderin-laden macrophages in bronchoalveolar lavage. After high dose intravenous steroid, cyclophosphamide intravenous therapy and plasmapheresis, the condition of patient was markedly improved. He was discharged and received monthly intravenous pulse cyclophosphamide. He has done well since, showing no further pulmonary hemorrhage with steroid tapering.

Slug suppression induces apoptosis via Puma transactivation in rheumatoid arthritis fibroblast-like synoviocytes treated with hydrogen peroxide

Hoon-Suk Cha,안중경,Jaejoon Lee,안광성,Eun-Mi Koh,Eun-Kyung Bae 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.6

Inadequate apoptosis contributes to synovial hyperplasia in rheumatoid arthritis (RA). Recent study shows that low expression of Puma might be partially responsible for the decreased apoptosis of fibroblast-like synoviocytes (FLS). Slug, a highly conserved zinc finger transcriptional repressor, is known to antagonize apoptosis of hematopoietic progenitor cells by repressing Puma transactivation. In this study,we examined the expression and function of Slug in RA FLS. Slug mRNA expression was measured in the synovial tissue (ST) and FLS obtained from RA and osteoarthritis patients. Slug and Puma mRNA expression in FLS by apoptotic stimuli were measured by real-time PCR analysis. FLS were transfected with control siRNA or Slug siRNA. Apoptosis was quantified by trypan blue exclusion, DNA fragmentation and caspase-3assay. RA ST expressed higher level of Slug mRNA compared with osteoarthritis ST. Slug was significantly induced by hydrogen peroxide (H2O2) but not by exogenous p53 in RA FLS. Puma induction by H2O2stimulation was significantly higher in Slug siRNA-transfected FLS compared with control siRNA-transfected FLS. After H2O2 stimulation, viable cell number was significantly lower in Slug siRNA-transfected FLS compared with control siRNA-transfected FLS. Apoptosis enhancing effect of Slug siRNA was further confirmed by ELISA that detects cytoplasmic histone-associated DNA fragments and caspase-3 assay. These data demonstrate that Slug is overexpressed in RA ST and that suppression of Slug gene facilitates apoptosis of FLS by increasing Puma transactivation. Slug may therefore represent a potential therapeutic target in RA.

Office Hysteroscopy의 임상적 유용성

고민환,이태형,김정숙,최윤영,정상훈 영남대학교 의과대학 2005 Yeungnam University Journal of Medicine Vol.22 No.1

자궁경술은 자궁강내를 직접 보고 생검하면서 수술 등을 할 수 있는 장점이 있어 부인과영역에서 진단 및 치료에 보다 더 정확하고 안전한 시술로써 인정받고 있으며 그 영역이 점차 확대되고 있다. 최근 20년간 내시경 영상과 수술기술, 수술도구의 발전으로 많은 진보가 있어 왔으며 이로 인해 office hysteroscopy가 가능하게 되었다. Office hysteroscopy는 입원하지 않고 당일 시술을 받을 수 있어 환자가 간편하고 안전하게 받을 있는 매우 유용한 술기로 여겨진다. 저자들은 1995년 9월부터 2005년 3월까지 시행한 자궁경술 140명 중 office hysteroscopy 18명을 경험하였기에 문헌고찰과 함께 보고하는 바이다. Background: Hysteroscopy is considered to be the gold standard not only for visualizing the cervical canal and the uterine cavity, but also for treating many different types of benign pathologies localized to those regions. The advent and evolution of endoscopic imaging and surgery during the last two decades has added new dimensions to the armamentarium of a gynecologist to combat intrauterine lesions. Office hysteroscopy is increasingly being used as a fast line investigation for abnormal uterine bleeding and other diseases involving the uterine cavity. The aim of our study is to assess the diagnostic and operative efficacy of office hysteroscopy. Materials and Methods: In our department, 140 patients underwent a hysteroscopy examination and 18 of these underwent an office based hysteroscopy examination from September 1995 to March 2005. The cases who underwent an office based hysteroscopy examination were reviewed in order to assess the clinical usefulness and significance in the management of intrauterine lesions. Results: Major indication was abnormal uterine bleeding(12 cases, 66.7%). The others were a missed IUD and infertility. The hysteroscopic findings were a normal uterine cavity(6 cases, 33.3%), lUD in situ, polyp, submucosal myoma, endometrial hyperplasia and a placenta remnant. Conclusion: Office hysteroscopy is a safe, quick and effective method for making an intrauterine evaluation. In addition, it provides immediate results, offers the capacity of direct targeted biopsies of suspicious focal lesions, and offers the direct treatment of some intrauterine conditions.

The Exposure Status and Biomarkers of Polycyclic Aromatic Hydrocarbons in Shipyard Workers

Koh, Sang-Baek,Park, Jun-Ho,Yun, Ju-Song,Lee, Kang-Myoung,Cha, Bong-Suk,Chang, Sei-Jin,Kim, Cheong-Sik,Kim, Heon,Chang, Soung-Hoon The Korean Society of Toxicogenomics and Toxicopro 2006 Molecular & cellular toxicology Vol.2 No.2

Because shipyard workers are involved with various manufacturing process in shipyard industry, and they are exposed to many kinds of hazardous materials. Especially, painting workers were exposed polycyclic aromatic hydrocarbons (PAH). This study was conducted to assess the exposure status of PAH based on job-exposure matrix. We investigated the effect of genetic polymorphism of xenobiotic metabolism enzymes involved in PAH metabolism on levels of urinary metabolite. A total of 93 shipbuilding workers were recruited in this study. Questionnaire variables were age, sex, use of personal protective equipment, smoking, drinking, and work duration. The urinary metabolite was collected in the afternoon and corrected by urinary creatinine concentration. The genotypes of CYP1A1, CYP2E1, GSTM1, GSTT1 and UGT1A6 were investigated by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods with DNA extracted from venous blood. Urinary 1-OHP levels were significantly higher in direct exposured group (spray and touch-up) than indirect exposed group. Urinary 1-OHP, concentration of the high exposure with wild type of UGT1A6 was significantlyhigher than that of the high exposure with other UGT1A6 genotype. In multiple regression analysis of urinary 1-OHP, the regression coefficient of job grade was statistically significant (p<0.05) and UGT1A6 was not significant but a trend (p<0.1). The grade of exposure affected urinary PAH concentration was statistically significant. But genetic polymorphism of xenobiotics metabolism enzymes was not statistically significant. Further investigation of genetic polymorphism with large sample size is needed.

만숙성 감귤 품종 ‘탐도3호’ 육성

박석만(Suk Man Park),윤수현(Su Hyun Yun),고상욱(Sang Wook Koh),이동훈(Dong Hoon Lee),김민주(Min Ju Kim),최영훈(Young Hun Choi),박재호(Jae Ho Park) 한국육종학회 2018 한국육종학회지 Vol.50 No.1

새로운 감귤 품종 ‘탐도3호’는 2004년 국립원예특작과학원 감귤연구소에서 ‘청견’(Citrus unshiu × C. sinensis)을 교배모본(종자친)으로 하고 ‘Sunburst’(Citrus reticulate)를 교배부본(화분친)으로 교배하여 얻어진 교잡품종이다. 탐도3호는 2010년에 첫 착과가 되었으며 2012년까지 포장 평가를 실시하였다. 탐도3호의 수세는 강하며 풍산성이다. 열매는 평균적으로 230g이며, 과실의 모양은 작은 봉을 가진 납작한 편구형이다. 착색은 10월 중순부터 시작되어 2월 초에 완전히 착색되며 그 색은 짙은 오렌지색이다. 플라스틱 하우스 재배 시 성숙기는 2월 중순이다. 과실의 당도와 산도는 각 각 14.0°와 1%이다. 과피의 두께는 평균 2.5mm로 온주밀감보다 얇아 과피 벗김이 쉽다. 이 품종은 자가불화합성과 단위결과성이 있어 단독 재배 시 종자가 발생되지 않으나 다른 종의 꽃가루가 수정되면 종자를 형성한다. 탐도3호는 노지 재배 시 과실이 냉해를 받기 때문에 플라스틱 하우스 재배가 요구된다. A new citrus cultivar ‘Tamdosamho’ is a hybrid obtained from a cross of ‘Kiyomi’ (Citrus unshiu×Citrus sinensis, the seed parent) and ‘Sunburst’ (Citrus reticulate, the pollen parent) at the Citrus Research Institute of the National Institute of Horticultural and Herbal Science in 2004. ‘Tamdosamho’ had the first fruit set in 2010 and field evaluation has been conducted until 2012. The tree of ‘Tamdosamho’ was vigorous and had a lot of fruit bearing. The average fruit weight was 230g and its shape was flatten-oblate with a little neck. The fruit peel color was dark orange, and peel coloration began in mid-October and fully developed in early December. The fruit matured in mid-February from cultivation under plastic film house. Total soluble solid and acidity of which was 14.0 °Brix and about 1 % respectively. The thickness of the rind was about 2.5 mm on average and was thinner than that of satsuma mandarin (Citrus unshiu), so removing the peel was easy. This cultivar had characteristic of self-incompatibility and parthenocarpy and didn’t bear seeds from monoculture but if it be fertilized by the pollen of other cultivars could be seedy. In the case of cultivation in the open field, the fruits could be damaged by cold temperature and then ‘Tamdosamho’ is recommended for cultivation under plastic film house. (Variety registration number: 6158)

류마티스 및 골관절염 섬유모세포양 활막세포에서 저산소증에 의한 Matrix Metalloproteinase 및 Tissue Inhibitor of Metalloproteinase-1 발현의 조절

차훈석 ( Hoon Suk Cha ),전찬홍 ( Chan Hong Jeon ),최화정 ( Hwa Jung Choi ),안광성 ( Kwang Sung Ahn ),송영욱 ( Yeong Wook Song ),고은미 ( Eun Mi Koh ) 대한류마티스학회 2003 대한류마티스학회지 Vol.10 No.2

Objective: The rheumatoid synovium is a hypoxic environment and hypoxia has been implicated to have a role in the pathogenesis of rheumatoid arthritis (RA). In this work, we tried to investigate the effect of hypoxia on the expression of matrix metalloproteinase (MMP)-1, -3 and tissue inhibitor of metalloproteinase (TIMP)-1 in rheumatoid and osteoarthritis (OA) fibroblast-like synoviocytes (FLS). Methods: FLS obtained from RA and OA patients were cultured under normoxic or hypoxic condition for 48 hours. Western blot analyses for MMP-1, -3 and TIMP-1 were performed. TIMP-1 mRNA levels were measured by Northern blot analysis. Results: Hypoxia increased MMP-1 expression in rheumatoid FLS compared with normoxia. TIMP-1 expression was decreased by hypoxia in rheumatoid FLS in both protein and mRNA levels. On the other hand, hypoxia did not significantly affect MMP-1, -3 and TIMP-1 expressions in OA FLS. Conclusion: These results suggest that microenvironment such as hypoxia may directly contribute to joint destruction in RA by increasing the ratio of MMP-1 to TIMP-1 production in FLS.