鬱血性心不全症에 對한 臨床的 觀察

徐明洙,金吉洙,金佑泰,張錫源,金承源,宋昌燮 대한적십자사 1983 적십자병원지 Vol.10 No.2

상피종양의 keratin 생성능에 대한 전자현미경적 연구

이길선,이명주,장정수,이민전,노광을,서재홍 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Keratins are characteristically present in all keratinizing and nonkeratinizing epithelial cells and their neoplasms. They consist of at least 19 different polypeptides ranging from 40 to 69 kilodaltons, which are developmentally related to specific epithelial cell types. Keratins of high and intermediate molecular weights are readily demonstrated in squamous cell carcinomas, which are characteristically rich in tonofilaments and tonofilament-desmosome complexes. Keratins of low molecular weights are expressed in adenocarcinoma including renal cell carinoma, hepatocellular carcinoma, small cell carcinoma, and carcinoid tumor. These tumors do not reveal any tonofilaments and sacrcely show discrete filaments on electron microscopy. An immunoperoxidase technique employing antibody to keratin was used to study distribution and staining pattern of keratin filaments in benign and malignant epithelial tumors (20 squamous cell carcinomas, 20 gastric adenocarcinomas, 20 transitional cell carcinomas and 15 non-neoplastic epithelia). These immunohistochemical results were compared with ultrastructural features of neoplastic diagnosis of squamous cell carcinoma, adenocarcinoma and transitional cell carcinoma, and estimation of degree of differentiation. The results are as follows : 1. The squamous epithelium, glandular epithelium and transitional epithelium disclosed the positive reaction for keratin. 2. Squamous cell carcinoma and transitional cell carcinoma exhibited positive reaction for keratin. 3. Gastric adenocarcinoma showed uniformly negative or weakly positive reaction for keratin. 4. On electron microscopy, heavy bundles of tonofilaments and desmosomes were more frequently encountered in well differentiated squamous cell carcinoma, In poorly differentiated squamous cell carcinoma, a few tonofibrils and desmosome were noted. 5. In the adenocarcinomas, the intermediate filaments were arranged randomly as nonaggregated, short filaments spread throughout the cytoplasm, although occasionally they formed a perinuclear whorl. These filaments did not become aggregated to form tonofilaments. 6. On the immunogold labeling for keratin of low molecular weight areas in gastric adenocinoma, in tense labeling of intermediate filaments for keratin was noted. From the above result, ultrastructural and immunohistochemical study on malignant epithelial cell were useful in differential diagnosis of squamous cell carcinoma, adenocarcinoma and transitional cell carcinoma, and estimation of degree of differentiation. It was also proved that immunogold labeling technique was especially valuable for diagnosis of vague case which showed negative or weakly positive reaction in immunohistochemical stain.

강원도 가칠봉의 식물자원

이재훈,장수길,유기억 강원대학교 환경연구소 2004 Journal of the Environment Vol.1 No.-

Plant resources of the Gachilbong were investigated from May, 2004 to October, 2004. Vascular plants of Gachilbong were composed of 86 families, 294 genera, 457 species 1 subspecies, 70 varieties and 10 formae, totaling 538 taxa. Twenty six species among the 538 species were Korean endemic, and the specific plants based on the distribution pattern appointed by Ministry of Environment were 96 taxa, and estimated 422 points. The naturalized plants were composed of 8 taxa. Life form spectra was H-D₄-R_(5)-e type, and the use of plant resources were composed edible 41.9%, pasture 35.7%, medicinal 32.9%, ornamental 10%, respectively.

Neuropeptide γ의 구조 및 생리활성

구희정,서정길,김은희,허민도,정준기,박장수,강신원,박남규 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-

생리활성을 지닌 신경펩타이드의 구조와 활성간의 관계를 규명하기 위하여 고상법으로 합성한 세종류의 neuropeptide γ(mammalian-,trout- 그리고 goldfish-neuropeptide γ)를 사용하여 연구하였다. Circular dichroism spectra에 의하면 mammalian-,trout- 와 goldfish-neuropeptide γ는 완충액 조건하에 모두 random한 구조를 나타내었다. 중성 및 산성 지질 존재 하에서, mammalian-과 trout- neuropeptide γ는 여전히 random한 구조를 취하였다. 그러나, goldfish-neuropeptide γ는 중성 및 산성지질하에서 부분적으로 α-helix구조를 나타내었다. 장관 수축활성에 있어서는 carp 장관, guinea-pig 회장 그리고 rat십이지장에 대하여 비교하였다. Carp에 대해서는 goldfish-neuropeptide γ= trout- neuropeptide γ>mammalian-neuropeptide γ순으로 활서이 나타났다. 그러나, guinea-pig 회장과 rat십이지장에 대해서 mammalian-neuropeptide γ는 어류 유래성 neuropeptide g들 보다 높은 수축활성을 나타내었다. 이러한 결과들은 neuropeptide γ들이 종-특이적인 활성을 나타낸다는 것을 제시한다. The relationship between structure and biological activity was studied on the three neuropeptides(mammalian-,trout- and goldfish-neuropeptide γ)that were syntheized by the solid-phase method. Circular dichroism spectra showed that mammalian-,trout- and goldfish-neuropeptide γadopted an undered structure in buffer solution. In the presence od neutral and acidic liposomes, mammalian-and trout- neuropeptide γ also took a random structure. However, goldfish-neuropeptide γ took an α-helical structure in acidic liposomes. The intestinal motility response was investigated with carp interstines, guinea-pig ileums and rat duodenums. In case of carp intestine, contractine activity was as follows:goldfish-neuropeptide γ= trout- neuropeptide γ> mammalian-neuropeptide γ. On the other hand, the contracile activity of mammalian-neuropeptide γ was more potent than trout- and goldfish-neuropeptide γ in the guinea-pig ileums and rat duodenums. these results suggest that neuropeptide γs showed the species-specific activity.

고추 조숙터널 직파재배시 멀칭재료 선발

허봉구,김하규,장길수,김주현 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.3

고추를 조숙터널재배 작형에서 직파할 때 터널지면을 멀칭하는 적정한 재료를 선발하기 위하여 2000년부터 2년간 영양고추시험장 시험포장에서 기온, 지온 등을 측정하고 고추의 생육과 수량을 조사하였으며 고추성분함량을 분석한 결과는 다음과 같다. 1. 직파한 고추의 입모율은 흑색P.E. 멀칭구에서 94%로 가장 높았으며 지중 10㎝에서의 최고, 최저온도는 투명P.E. 멀칭구가 가장 높았다. 2. 고추 초기생육은 흑색P.E. 멀칭구가 가장 좋았으나 후기생육은 처리간에 큰 차이가 없었다. 3. 고추 수량은 흑색P.E. 멀칭구 〉투명P.E. 멀칭구 〉배색P.E. 멀칭구 순으로 높았으며 과실 특성은 비슷하였다. 4. 흑색P.E. 멀칭구의 ASTA값이 195.7로 가장 높았으며 당함량은 투명P.E. 멀칭구가 8.7%로 높았다. This experiment was conducted to select the mulching materials at the direct sowing of red pepper in the early maturing culture under row cover. 'Taeyang' cultivar was planted at the March 27 by driect sowing. Mulching material were black P.E., clear P.E. and arranged P.E., Soil temperature at 10㎝ depth measured by Watch Dog (Spectrum Technologies Inc.) during the cropping seasons. Planting density was 4,444 plants per 10a, double row design with 0.3m between plants within the row and 1.5m with. Fertilization application on the red pepper was standard fertilization which RDA recommended. Seedling stand ratios of red pepper in the black P.E. plot was higher by 94% than the others. Soil temperature at 10㎝ depth including maximum and minimum temperatures in the clear P.E. plot were higher than those of another plots. Red pepper growth in the early stage was better in the black P.E. plot than the other plots. In the late stages, the red pepper growth to black P.E. 〉clear P.E. 〉arranged P.E. plot, but fruit characteristics were similiar. ASTA value of black P.E. plot was higher by 195.7 than the other plots. Sugar content of clear P.E. plot was 8.7%.

만성 골수성 백혈병(Chronic Myelogenous Leukemia) 치료 중 인터페론-알파(Interferon-α)에 의한 용혈성 요독 증후군 1예

장재원,김유미,서장원,최귀숙,유수진,지현숙,유종우,박수길 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2

Interferon-alpha is an established therapy for the patients with myeloproliferative disease. Unusual immune- mediated side effects have been reported during treatment. Among them, renal and cardiac toxicities are rare complications. Recently we experienced a case of hemolytic uremic syndome found in a 40-year old male undergoing interferon-alpha therapy for chronic myelogenous leukemia, chronic phase. On admission, his chief complaints were generalized edema and azotemia. Bone marrow biopsy revealed transformation into accelerated phase. bcl/abl gene was positive. Peripheral blood smear showed hemolytic anemia. Despite prompt diagnosis, institution of plasmapheresis, and steroid, his renal function did not recover, as expected. Hemolytic uremic syndrome was a chronic form of renal toxicity due to interferon- alpha. In this case, renal function did not recovered to normal level. However, early detection of renal toxicity such as proteinuria or acute tubular necrosis, and the discontinuation of interferon-alpha could achieve the recovery of normal renal function. Therefore, periodic examination of renal function should be performed in the patients with the use of long-term interferon-alpha. (Korean J Nephrol 2002; 21(2):341-347)

Decoction and Fermentation of Selected Medicinal Herbs Promote Hair Regrowth by Inducing Hair Follicle Growth in Conjunction with Wnts Signaling

Jang, Su Kil,Kim, Seung Tae,Lee, Do Ik,Park, Jun Sub,Jo, Bo Ram,Park, Jung Youl,Heo, Jong,Joo, Seong Soo Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-

<P>It is well recognized that regulating the hair follicle cycle in association with Wnt signaling is one of the most interesting targets for promoting hair regrowth. In this study, we examined whether selected herbal medicines processed by decoction and fermentation promote hair growth by upregulating the number and size of hair follicles and Wnt signaling, including activation of <I>β</I>-catenin and Akt in telogen-synchronized C57BL/6N mice. The results revealed that the fermented extract after decoction (FDE) more effectively promoted hair growth than that of a nonfermented extract (DE). Notably, FDE effectively enhanced formation of hair follicles with clearer differentiation between the inner and outer root sheath, which is observed during the anagen phase. Mechanistic evidence was found for increased <I>β</I>-catenin and Akt phosphorylation levels in dorsal skin tissue along with elevated expression of hair regrowth-related genes, such as Wnt3/10a/10b, Lef1, and fibroblast growth factor 7. In conclusion, our findings suggest that FDE plays an important role in regulating the hair cycle by increasing expression of hair regrowth-related genes and activating downstream Wnt signaling targets.</P>

Promotional effects of Sargassum fusiforme fractions on hair growth via in vitro and in vivo models

Su Kil Jang, Seong Soo Joo 충북대학교 동물의학연구소 2014 Journal of Biomedical and Translational Research Vol.15 No.2

Sargassum fusiforme has traditionally been widely consumed in Asia as a food, and it has gained much attention due to its high nutritional, pharmaceutical, and industrial value. This study aimed to examine the promotional effects of ethanol extract (ET) and fraction obtained from ethyl acetate (FR) of S. fusiforme on hair growth in C57BL/6 mice and HaCaT cells. Five-week-old mice were used to compare hair regrowth during application of ET and FR for 21 days. Hair regrowth was evaluated by macroscopic observation and verified by hematoxylin-eosin tissue staining. Levels of mRNA expression of factors relevant to the hair growth cycle such as keratinocyte growth factor (KGF), vascular endothelial growth factor (VEGF), and transforming growth factor-beta1 (TGF-β1) were examined by quantitative polymerase chain reaction (qPCR). Our results showed that ET and FR successfully promoted hair regrowth in shaved C57BL/6 mice at a dose >20 mg/kg. Moreover, ET and FR were effective in stimulating expression of KGF and VEGF mRNAs in a dose-dependent manner, whereas TGF-β1 was not activated. These results indicate that ET and FR of S. fusiforme effectively promoted hair growth and gene expression relevant to hair growth cycles in both in vitro and in vivo models.

이상기후에 의한 고추 생육 및 병발생 실태

장길수(Jang Kil-Su),최동진(Choi Dong-Jin),김현석(Kim Hyun-Suk),김하규(Kim Ha-Kyu),이문중(Lee Mun-Jung),권택륜(Kwon Taek-Ryoun),배도함(Pae Do-Ham) 한국원예학회 1999 원예과학기술지 Vol.17 No.5

논에서의 고추 재배 효과

장길수(Kil Su Jang),권오훈(Oh Hun Kwon),황지은(Ji Eun Hwang),원종건(Jong Gun Won),전수경(Su Gyeong Jeon),박상구(Sang Gu Park) 한국원예학회 2010 한국원예학회 학술발표요지 Vol.2010 No.5