Hypoxia-Responsive MicroRNA-101 Promotes Angiogenesis <i>via</i> Heme Oxygenase-1/Vascular Endothelial Growth Factor Axis by Targeting Cullin 3

Kim, Ji-Hee,Lee, Kwang-Soon,Lee, Dong-Keon,Kim, Joohwan,Kwak, Su-Nam,Ha, Kwon-Soo,Choe, Jongseon,Won, Moo-Ho,Cho, Byung-Ryul,Jeoung, Dooil,Lee, Hansoo,Kwon, Young-Guen,Kim, Young-Myeong Mary Ann Liebert 2014 Antioxidants & redox signaling Vol.21 No.18

<P>Aims: Hypoxia induces expression of various genes and microRNAs (miRs) that regulate angiogenesis and vascular function. In this study, we investigated a new functional role of new hypoxia-responsive miR-101 in angiogenesis and its underlying mechanism for regulating heme oxygenase-1 (HO-1) and vascular endothelial growth factor (VEGF) expression. Results: We found that hypoxia induced miR-101, which binds to the 3 ' untranslated region of cullin 3 (Cul3) and stabilizes nuclear factor erythroid-derived 2-related factor 2 (Nrf2) via inhibition of the proteasomal degradation pathway. miR-101 overexpression promoted Nrf2 nuclear accumulation, which was accompanied with increases in HO-1 induction, VEGF expression, and endothelial nitric oxide synthase (eNOS)-derived nitric oxide (NO) production. The elevated NO-induced S-nitrosylation of Kelch-like ECH-associated protein 1 and subsequent induction of Nrf2-dependent HO-1 lead to further elevation of VEGF production via a positive feedback loop between the Nrf2/HO-1 and VEGF/eNOS axes. Moreover, miR-101 promoted angiogenic signals and angiogenesis both in vitro and in vivo, and these events were attenuated by inhibiting the biological activity of HO-1, VEGF, or eNOS. Moreover, these effects were also observed in aortic rings from HO-1(+/-) and eNOS(-/-) mice. Local overexpression of miR-101 improved therapeutic angiogenesis and perfusion recovery in the ischemic mouse hindlimb, whereas antagomiR-101 diminished regional blood flow. Innovation: Hypoxia-responsive miR-101 stimulates angiogenesis by activating the HO-1/VEGF/eNOS axis via Cul3 targeting. Thus, miR-101 is a novel angiomir. Conclusion: Our results provide new mechanistic insights into a functional role of miR-101 as a potential therapeutic target in angiogenesis and vascular remodeling. Antioxid. Redox Signal. 21, 2469-2482.</P>

우리나라 長壽者의 生活 및 意識調査에 관한 硏究 - Ⅴ. 過去의 健康疾病 및 苦痛에 대한 態度 -

최진호(Jin-Ho Choi),변재형(Jae-Hyeung Pyeun),임채환(Chae-Hwan Rhim),양종순(Jong-Soon Yang),김수현(Soo-Hyun Kim),김정한(Jeung-Han Kim),이병호(Byeong-Ho Lee),우순임(Soon-Im Woo),최선남(Sun-Nam Choe),변대석(Dae-Seok Byun),김무남(Mu-Nam 군산대학교 수산과학연구소 1994 군산대학교 수산과학연구소 연구논문집 Vol.1 No.-

The present study was designed to be link a part of the research on the investigation on daily life and consciousness of the longevous people in Korea, and to investigate the health conditions, and the attitude on disease and suffering of longevous people in the past. 1. By the health conditions of longevous people between 40 and 60 years, ""very good"" showed the highest figure of 71.0%, followed by ""ordinary"" (21.6%). Consequently it is found that 92.6% of total longevous people surveyed maintained the good health conditions above ordinary. 2. Of the experiences of disease and/or injury after 60 years of longevous people, ""never"" showed the highest figure of 74.7%, while ""had"" showed the only 22.6% of total longevous people surveyed. 3. Of the details of disease and/or injury after 60 years of longevous people. ""digestive disease"" showed the highest figure of 32.6%. followed by ""apoplexia cerebri & hypertensions""(14.0%) and ""heart disease""(14.0%), and followed by ""respiratory disease""(12.3%). 4. Of the experiences of suffering between 40 and 60 years of longevous people, ""never"" showed 53.7% of total longevous people surveyed, while the frequency of suffering decreased in the order of ""once""(22.7%), ""2 times""(15.8%) and ""3 times""(4.7%). By the details of suffering between 40 and 60 years of longevous people. ""family life"" showed the highest figure of 85.1%, followed by ""business""(10.1%). 5. By the attitude on suffering of longevous people, ""tried to forget soon"" showed the highest figure of 54.2%, followed by ""continuous worried""(23.3%), and followed by ""not worried""(7.2%).

Red pepper(Capsicum annuum L.) 增收를 爲한 計劃灌漑 理論 適用 및 實證試驗에 關한 硏究

胡敎純 尙志大學校 1986 論文集 Vol.7 No.-

. Studies on the Application of Theory and Practice of Scheduling Irrigation to Increase Yield of Red Pepper (Capsicum annuum L.) Qyo-Soon, Ho. This study was carried out to applicate of theory in planning, design and operation of scheduling irrigation in order to increase yield of red pepper. The results were as follows. 1. Irrigation date and irrigation supply requirements were caculated considering the climatic data, soil servey data, crop and reference evapotranspiration. There are shown as follows. ================================================================================================ month April May June July August September ------------------------------------------------------------------------------------------------ Date 30 11 23 27 2 12 23 25 29 1 9 18 PIS(mm) 10.0 16.5 16.6 - 18.0 18.0 17.8 25.0 - 24.0 24.0 22.5 APIS(mm) 100% 10.0 - - 16.5 18.0 18.0 17.8 - 20 - - - ------------------------------------------------------------------------------------------------ 2. The most effective treatment were compared to control was the plot of APIS 100% irrigation supply and vinyl mulching treated plot, in case of the plant height, stem node numbers of flower bud development, flower blooming and fruit setting. 3. In the plot of treated with APIS 100% irrigation supply, the number and fresh weight of red pepper was increased, about 70%, as much as than control plot, and the treated with vinyl mulching, was increased about 55%, and other treatment was increased from 5% to 22.8%. 4. Yield of dry weight of red pepper from APIS 100, 130% irrigated plot and vinyl mulching treated plot were increased 77.6, 59 and 58%, respectively as compared to the yield of control plot whereas yield from APIS 70 and 50% were decreased 18 and 20% respectively. The results showed that better yield of red pepper should be obtained by adequate water supply under the scheduling irrigation.

우리나라 長壽者의 生活 및 意識調査에 관한 硏究 - Ⅴ. 過去의 健康疾病 및 苦痛에 대한 態度 -

최진호(Jin-Ho Choi),변재형(Jae-Hyeung Pyeun),최재수(Jae-Sue Choi),임채환(Chae-Hwan Rhim),김수현(Soo-Hyun Kim),김정한(Jeung-Han Kim),이병호(Byeung-Ho Lee),우순임(Soon-Im Woo),최선남(Sun-Nam Choi),변대석(Dae-Seok Byun),김무남(Mu-Nam Ki 한국식품영양과학회 1987 한국식품영양과학회지 Vol.16 No.4

우리나라 長壽者의 生活 및 意識調査硏究의 一環으로 長壽者의 過去의 健康, 疾病 및 苦痛에 대한 對處態度 등을 調査ㆍ比較한 結果는 다음과 같다.<br/> 1. 更年期(40~60歲)의 健康狀態가 『대단히 건강했다.』가 71.0%로 가장 많고, 그 다음이 『건강이 보통이었다』가 21.6%로서, 전체 장수자의 91.6%가 보통이상의 건강상태를 유지했다.<br/> 2. 老年期에 접어든 후 疾病이나 負傷을 당한 經驗이 『없었다』고 대답한 경우가 74.7%로 대부분을 차지한 반면 『있었다』고 대답한 경우는 22.6%에 불과했다.<br/> 3. 疾病이나 負傷이 『있었다』고 한 경우의 內譯을 比較하여 보면 『消火器疾患』이 32.6%로 가장 많고, 그 다음이 『腦率中ㆍ高血壓』 14.0%, 『心臟病』 14.0%, 『呼吸器疾患』 12.8%의 순으로 되어 있었다.<br/> 4. 苦痛에 대한 經驗을 調査하여 본 結果, 苦痛이 『없었다』가 55.7%, 『있었다』가 44.3%로 거의 비슷한 傾向이었고, 苦痛에 대한 經驗內譯을 보면 『가정생활』이 85.1%로 대부분을 차지한 반면 『사업』 등은 10.1%에 불과해서 가정생활에 많은 문제가 있었음을 알 수 있었다.<br/> 5. 苦痛에 대한 對處態度롤 比較하여 보면 빨리 『잊을려고 努力했다』가 51.2%로 가장 많았고 그 다음이 『계속 걱정을 했다』(33.3%), 『걱정을 하지 않았다』(7.1%)의 순이었다.<br/> 6. 長壽한 사람들의 初經年齡은 『16~18歲』가 38.8%로 가장 많고, 그 다음이 『19歲이후』가 25.2%로서 전체 장수자의 64.0%가 16세이상에서 初經이 나타났으며, 閉經年齡은 『41~43歲』가 36.4%로 가장 많고, 그 다음이 『44~46歲』가 28.5%로서 전체 장수자의 64.9%가 46歲이하에서 閉經現象이 나타났다. 따라서 장수한 사람들의 初經年齡은 현재보다 4~5年 늦은 반면 閉經年齡은 현재보다 4~5年 빨랐음을 알 수 있었다. The present study was designed to be link a part of the research on the investigation on daily life and consciousness of the longevous people in Korea, and to investigate the health conditions, and the attitude on disease and suffering of longevous people in the past.<br/> 1. By the health conditions of longevous people between 40 and 60 years, "very good" showed the highest figure of 71.0%, followed by "ordinary" (21.6%). Consequently it is found that 92.6% of total longevous people surveyed maintained the good health conditions above ordinary.<br/> 2. Of the experiences of disease and/or injury after 60 years of longevous people, "never" showed the highest figure of 74.7%, while "had" showed the only 22.6% of total longevous people surveyed.<br/> 3. Of the details of disease and/or injury after 60 years of longevous people, "digestive disease" showed the highest figure of 32.6 %, followed by "apoplexia cerebri & hypertensions" (14.0%) and "heart disease" (14.0%), and followed by "respiratory disease" (12.8%).<br/> 4. Of the experiences of suffering between 40 and 60 years of longevous people, "never" showed 55.7% of total longevous people surveyed, while the frequency of suffering decreased in the order of "once" (22.7%), "2 times" (15.8%) and "3 times" (4.7%). By the details of suffering between 40 and 60 years of longevous people, "family life" showed the highest figure of 85.1%. followed by "business" (10.1%).<br/> 5. By the attitude on suffering of longevous people, "tried to forget soon" showed the highest figure of 51.2%, followed by "continuous worried" (33.3%). and followed by "not worried" (7.2%).

In vitro and in vivo anti-inflammatory effects of pegmatite

Jo, Wol-Soon,Yang, Kwang-Mo,Choi, Yoo-Jin,Jeong, Chang-Hwa,Ahn, Kyoung-Jin,Nam, Byung-Hyouk,Lee, Sang-Wha,Seo, Su-Yeong,Jeong, Min-Ho The Korean Society of Toxicogenomics and Toxicopro 2010 Molecular & cellular toxicology Vol.6 No.2

Pegmatite is a coarse-grained intrusive igneous rock rich in rare elements such as uranium, tungsten, and tantalum with Ca, K, Mg, Fe, Se, Ge, and Ho. We tested in vitro and in vivo assays for the anti-inflammatory activity of pegmatites. We firstly evaluated the suppressive effects of pegmatite on macrophage cell line RAW 264.7 cells stimulated with proinflammatory stimuli lipopolysaccharide (LPS) to determine nitric oxide (NO) production and TNF-$\alpha$ and IL-6 release. The $IC_{50}$ values of pegmatite exceeded $5,000\;{\mu}g/mL$. Treatment of RAW 264.7 cells with pegmatite significantly inhibited LPS-stimulated NO production and proinflammatory cytokines such as TNF-$\alpha$ and IL-6 secretion in a dose-dependent manner (P<0.05). In vivo studies were tested with two animal models of arachidonic acid-induced mouse ear edema and an acetic acid-induced increase in capillary permeability. The pegmatite significantly attenuated ear edema induced by arachidonic acid and reduced the acetic acid-induced increase in capillary permeability in mice (P<0.05) when the pegmatite was administered topically (10 mg per ear) for 24 h. Therefore, pegmatite potentially shows an anti-inflammatory activity in the in vitro and in vivo mice and in the development of newer anti-inflammatory agents as mineral materials.

한국인 본태성 고혈압환자에서 Angiotensinogen M235T 유전자 다양성

양동호(Dong Ho Yang),홍세용(Sea Yong Hong),김홍수(Hong Soo Kim),방차옥(Cha Ok Bang),김철현(Chul Hyun Kim),최종순(Jong Soon Choi),하권수(Kwun Soo Ha) 대한신장학회 2000 Kidney Research and Clinical Practice Vol.19 No.3

N/A Background: Potential involvement of the angio-tensinogen gene(M235T) in the pathogenesis of essen-tial hypertension has been suggested by some inves- tigators. However, an association between M235T gene polymorphism and essential hypertension has been reported by some, but not by others. Since genetic diversity exists among different ethnic population, we addressed the question of whether there is an association between M235T gene poly-morphism and essential hypertension in the Korean. Methods: 100 patients with essential hypertension and 100 control subjects were recruited from out- patients at the Department of Internal Medicine, Soon-chunhyang Hospital, Chunan City. The criteria for hypertension was defined as systolic blood pressure higher than 160mmHg and/or diastolic blood pressure higher than 95mmHg. For detection of the M235T polymorphism at the angiotensinogen locus, the primer sequences were: sense primer : 5'-TGAAGGAG- AAGGTGTCTGCGGGA-3' and antisense primer : 5'- AGGACGGTGCGGTGAGAGTG-3'. The PCR product mixture was exposed to restriction enzyme Tthlll I and then submitted to electrophoresis in polyacryl-amide gel. Differences between the molecular variants of the gene in hypertensives and normotensives were com- pared by using the X²test. p<0.05 was considered statistically significant. The odds ratio and 95% confidence interval were calculated using Woolf's method. Results: Compared with the control subjects, hypertensives had higher values of three established risk factors for hypertension : age, BMI cholesterol (total and LDL). X analysis showed no difference in the distribution of genotype or allele frequency between the hypertensives and normotensives(X²=1.14, p=0.29). The crude odds ratio was 0.73 for CC over CT(95% confidence interval 0.41-1.30). The adjusted odds ratio with age, sex, lipid profilcs and BMI was 0.68 for CC over CT(9596 confidence interval 037-1,23). Conclusion : The molecular varient M235T of the angiotensinogen gene is not associated with essential hypertension in Korean population.

정인섭 동요와 악곡 연구

김정호 ( Kim Joung-ho ),이순욱 ( Lee Soon-wook ) 경남대학교 인문과학연구소 2021 人文論叢 Vol.56 No.-

이 글은 식민지시대 울산 출신 정인섭의 색동회 활동과 동요 창작의 현황을 파악하고 악곡을 분석하는 데 목적을 두었다. 이 시기 동요는 신문이나 잡지 등 근대 매체를 통해 창작되고 향유되면서 대중적으로 빠르게 보급되고 안착하였다. 즉 동요는 어린이잡지의 독자투고란이나 일간지의 학예면을 통해서 독자와 만나고 점차 자기 위상을 확립하게 된 것이다. 당시 어린이잡지인 『어린이』의 경우 ‘동요란’을 개설하였으며, 일간지 『동아일보』의 경우에도 학예면의 가장 많은 부분을 차지하는 장르는 동요였다. 그만큼 동요에 대한 독자들의 열망이 높았다. 그만큼 매체가 동요의 대중화에 큰 역할을 수행했던 것이다. 이러한 현실적 토대에서 전문적인 동요작가들이 등장하였으며, 울산에서도 정인섭을 비롯하여 서덕출, 오영수, 신고송 등이 등장하였다. 이들이 남긴 ‘동요’의 문학사적 위치를 실증적으로 추적하는 일은 매우 중요하다고 하겠다. 특히 정인섭은 ‘색동회’와 ‘녹양회’의 회원으로 활동하고, 어린이잡지 『어린이』의 필진으로 참여하는 등 어린이문화운동에 앞장선 인물이다. 정순철 동요집 『참새의 노래』에 수록된 <참새>, <꿈 노루>, <코끼리 코>, <처마 끗헤 새 한 마리>, <굴뚝쟁이>, <가을 나븨>, <설날>과 강신명이 엮은 『아동가요곡집』에 수록된 <봄노래>, <보건체조가> 등은 어린이들을 위한 동심천사주의와 교훈주의, 계몽주의를 잘 드러내고 있으며, 가사의 운율을 음악적 선율로 살려 가사의 의미를 자연스럽게 표현하도록 하였다. 이러한 정인섭의 동요 보급은 어린이문화운동에 기여하였고, 그의 가곡은 아직도 연주회에서 불리는 등 예술적으로 가치를 인정받고 있어 음악사적으로 의의가 크다. The purpose of this article is to analyze the current situation of the creation of songs and the activities of Jeong In-seop’s Saikdonghoi(색동회) movement in Ulsan during the colonial period. The most important part of the media was that the agitation quickly settled into the modern genre. The children’s song, which was created and enjoyed by the previous oral tradition, began to be created and enjoyed through typed media such as newspapers and magazines in modern times. The popular distribution was also made by such media: children’s literature magazines’ readers’ comments or daily journals’ academic section, which led to the meeting with readers and gradually establishing their own status. At that time, readers’ desire for children’s songs was high enough to open ‘swaying’ independently in the case of children, and the genre of Dong-A Ilbo, which occupies the largest part of the curriculum, was children's songs. Therefore, this media plays the biggest role in popularizing agitation. In this realistic foundation of the time, professional children’s songs were born, and Jung In-seop, Seo Deok-chul, Oh Young-soo, and Shin-Go-song were born in Ulsan. It is very important to empirically track the literary history of ‘single’ about them.,In particular, Jung In-seop has been a member of the ‘Saikdonghoi(색동회)’ and ‘Nokyanghoi(녹양회)’ and has led the children's cultural movement by participating as a writer for children’s magazines. The songs of Jung Soon-chul's song of the sparrows include “Sparrow”, “Dream Noru”, “Elephant Nose”, “A Shrine Bird”, “Chimney”, “Autumn Na”, “New Year” and “Spring Song” and “Health Gymnastics” It shows the meaning of the lyrics naturally by utilizing the rhythm of the lyrics with musical melody.,This spread of children’s songs by Jung In-seop contributed to the children’s cultural movement, and his songs are still called in concerts, and their value is recognized artistically, which is of great significance in the history of music.

복어중독에 의한 가사 상태에서 소생한 1예

송승찬,신진호,강석우,박경남,최호순,박근태,문희식,기춘석,이성희,윤병철,노우균,조균석,이민호 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.3

Tetrodotoxin is a neurotoxin produced by about 90 species of puffer fish and causes paralysis of central nervous system and peripheral nerves by blocking the movement of all monovalent cation. Ingestion of tetrodotoxin produces clinical manifestations such as paresthesias(within 10-45 min), vomiting, lightheadedness, salivation, muscle twitching, dysphagia, difficulty in speaking, convulsion and death that expressed by cardiopulmonary arrest with loss of brain stem reflex sometimes. Tetrodotoxin prevents or delays ischemia induced neuronal death by way of following 3 mechanisms. Firstly, it reduces the energy demand of the brain tissues. Secondly, it delays or even prevents anoxic depolarization. Finally, it deminishes ischemia induced cell swelling and cerebral edema. We report a case of puffer fish poisoning which presented with cardiopulmonary arrest and loss of brain stem reflex, but completely recovered by aggressive cardiopulmonary resuscitation.

8년간(1992~1999) 산업안전보건연구원에 의뢰된 직업병 심의 사례 분석

강성규,김규상,김양호,최정근,안연순,진영우,최병순,양정선,김은아,채창호,최용휴,김대성,박정선,정호근 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2

Objectives : Pneumoconisis and noise-induced hearing loss (NIHL) have been reported as main occupational diseases by the Special Health Examination. The Industrial Accident Compensation Insurance has reported various work-related diseases, however, these two diseases occupied almost a half of compensated cases. Therefore, it was not well known about the status of occupational diseases other than pneumoconiosis, NIHL, and cardio-cerebrovascular accident (CVA). This study was conducted to analyze claimed cases as an occupational disease, that was requested to the Korea Industrial Safety and Health Agency (KOSHA). Methods : The local office of the Korea Labor Welfare Corporation (KLWC) has asked the KOSHA for confirmation of claimed cases as an occupational disease. We analyzed 379 cases requested from KLWC, the Ministry of Labor, employers, unions and occupational health agencies from 1992 to 1999. Results : Male was 80.7 % of the requested cases. Their mean age was 42 years old and 75.5 % of them were more than 35 years old. The requested cases were increased rapidly from 25 cases in 1992 to 108 cases in 1999 and the accept rate was 50.7 %. The majority of requested cases were respiratory diseases(22.4%), cancers(18.5%), Neuropsychiatric problems(14.5%), and musculoskeletal problems(13.5%). The accept rate was high in reproductive, respiratory, musculoskeletal and digestive disorders and low in neuropsychiatric, renal and otologic problems and occupational cancers. 73.6% of them were caused by chemical agents, especially 28.5 % were by organic solvents. 67% of them were clinically confirmed at university hospitals. A half of the cases were from KyongIn area, even the request came from the whole country. Conclusions : A claim was common in workers whose age was over 35 years old and exposure history was over 10 years. The respiratory diseases and neuropsychiatric disorders were still main problems in occupational health and occupational cancers was increasing even though its accept rate was not high yet.

Azithromycin으로 치료한 임신 중 쓰쓰가무시병 1예

김광석,최진욱,서호종,김기훈,박성호,서광섭,고성만,김순혜,김호정 대한감염학회 2001 감염 Vol.33 No.5

We report a case of tsutsugamushi disease in a 26 year-old pregnant woman who was treated with azithromycin. Her gestation period was 27 weeks and she admitted with fever, rash, and eschar on the right shoulder. Currently recommended medications for the treatment of scrub typhus are doxycycline or chloramphenicol. But, these drugs are class D drugs according to the U.S. Food and Drug Administration (FDA) Fetal Risk Summary, so they couldn't be used to treat pregnant women. Recently, a few case reports suggested that azithromycin, a relatively new macrolide antibiotic, was effective and safe for the treatment of scrub typhus in pregnant women. And, there is no evidence that azithromycin causes harmful effects to the developing fetus or to children. On the basis of current in vivo test that confirms the effectiveness of azithromycin, it may be the drug of choice for the treatment of scrub typhus in pregnant women and children. (Korean J Infect Dis 33:380-382, 2001)