Genetic Influences on Hallux Valgus in Koreans: The Healthy Twin Study

Lee, Chang-Hyung,Lee, Sooji,Kang, HyoJeong,Jung, Da-Eun,Song, Yun-Mi,Lee, Kayoung,Lee, Kyungtai,Hwang, JiHye,Sung, Joohon Cambridge University Press 2014 TWIN RESEARCH AND HUMAN GENETICS - Vol.17 No.2

<P>Hallux valgus (HV) is a common foot deformity of multifactorial etiology, but knowledge about the relative importance of genetics and environments on HV has been limited. In order to estimate genetic influences on HV, 1,265 adults, including 175 monozygotic twin (MZ) pairs, 31 dizygotic twin (DZ) pairs, and 853 first-degree singleton family members of the twins were included from the Healthy Twin study, a population-based twin-family cohort in Korea. All participants underwent foot examination and weight-bearing radiographic assessment (anterior-posterior and lateral) in addition to a general health survey. Of the subjects, 208 (16.4%) were classified as HV (as HV angle >20°). The genetic influence on HV was estimated to be substantial; the heritability of HV was 0.51 (95% CI 0.42-0.59) and the heritability of HV angle was 0.47 (0.38-0.56), while contributions from shared environmental effects were negligible. These findings suggest that genetic factors play an important role in determining HV deformity.</P>

Evaluation of Toxicity for Commercial Red Mud Pellets Using Pseudokirchneriella subcapitata and Daphnia magna

Lee, Saeromi,Ahn, Chang Hyuk,Park, Jae Roh,Lee, Sooji,Lee, Inju,Joo, Jin Chul Korean Society of Ecology and Infrastructure Engin 2015 Ecology and resilient infrastructure Vol.2 No.4

The toxicity of red mud (RM) pellets for water purification was evaluated using Pseudokirchneriella subcapitata and Daphnia magna in a lab-scale experiment. According to the algal growth inhibition test, both specific growth rates and relative growth rates of P. subcapitata decreased, and the growth inhibition rates increased ($R^2=0.97$, p<0.001) as the concentration of RM pellets in the aqueous solution increased (>1.6 g/L). Also, based on the acute toxicity evaluation test on D. magna, toxic unit (TU) values ranged between 0.00 and 2.83, and increased with an increase in the concentration of RM pellets in the aqueous solution. A correlation analysis indicated that the pH of RM pellets was statistically correlated with TU values ($R^2=0.77$, p=0.02). The environmental implication from this study is that the concentration of RM pellets in an aqueous solution needs to be lower than 4.4 g/L to keep the maximum permissible TU value less than 1.0.

Risk of Cardiovascular and Cerebrovascular Events in Hepatitis C Patients Following Completion of Direct-Acting Antiviral Therapy: A Retrospective Cohort Study

( Sooji Lee ),( Amanda W. Singer ),( Anu Osinusi ),( Diana M. Brainard ),( Anand P. Chokkalingam ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Chronic hepatitis C virus (HCV) infection has been associated with adverse cardiovascular and cerebrovascular outcomes. Viral eradication with direct-acting antiviral (DAA) therapy may decrease the risk of these events among HCV patients. We aimed to characterize the risk of cardiovascular and cerebrovascular events among HCV patients treated with DAA regimens compared to untreated patients in US claims data. Methods: 322,276 adults with chronic HCV were enrolled in the database between January 2006 and September 2015. We identified 8,342 HCV patients dispensed at least 12 weeks of DAA therapy (excluding boceprevir and telaprevir) and, for comparison, 76,423 untreated HCV patients who had follow-up time in the DAA era. Events were identified by diagnostic claims for acute and chronic ischemic heart disease, angina pectoris, cardiomyopathy, heart failure, subarachnoid hemorrhage, occlusion and stenosis of precerebral or cerebral arteries, cerebrovascular disease, cerebral atherosclerosis, intracerebral hemorrhage, and transient cerebral ischemia. Hazard ratios (HRs) estimating risk of incident cardiovascular and cerebrovascular events associated with completion of DAA therapy were calculated with adjustment for covariates using Cox proportional hazards methods. Results: HCV patients dispensed a full course of DAA therapy were more likely to be male, over 55 years-old, with baseline diagnoses of cirrhosis, diabetes, or hypertension, and on cardiovascular medications. After adjustment for covariates, there was a reduced risk of total cardiovascular and cerebrovascular events in patients completing DAA therapy compared to untreated patients (HR=0.86, 95% confidence interval [CI]: 0.74-0.99). Adjusted HRs were similar for cardiovascular disease (HR=0.90, 95%CI: 0.76-1.05) and cerebrovascular disease (HR=0.89, 95%CI: 0.74-1.08). Conclusions: In this real-world cohort, DAA therapy appeared to reduce the risk of cardiovascular and cerebrovascular events in HCV patients even within a short period following therapy. The benefits of curative DAA therapy in reducing extrahepatic complications of HCV may be even greater with longer follow-up.

Preventive effect of Lumbrokinase on post-surgical intra-abdominal adhesion

( Sooji Ham ),( Bo-ra Lee ),( Ji Hye Kim ),( Jin-a Oh ),( Dhan-ah Chae ),( Yeojin Lee ),( Hyun Jin Choi ),( Eun-ju Lee ) 대한산부인과학회 2020 대한산부인과학회 학술대회 Vol.106 No.-

Objective: Postoperative adhesion is a major cause of chronic pain, infertility, bowel obstruction, and urinary dysfunction. Normally peritoneum is covered with monolayer of mesothelial cells which release plasminogen activator. Postoperative adhesion is provoked by increase of plasminogen activity inhibitors and decrease of fibrinolytic activity, which led to failure of fibrin exudate degradation and adhesion removal. This study is aimed to assess the effect of lumbrokinase (LK), a fibrinolytic enzyme extracted from earthworm, on prevention of postoperative adhesion. Methods: Intrabdominal injuries were induced with scratching until spot bleeding on peritoneum wall and cecum of Sprague-Dawley rats and lumbrokinase were intraperitoneally applied in injured area. Coagulation and hematological profiles were assessed after LK treatment. After 2 weeks after treatment, the severity and the area of intraabdominal adhesion were assessed with scoring system and immunohistochemical analysis. MTT assay and scratch migration assay were used to determine the effect of LK on fibroblast. Results: There is no adverse event after LK treatment. The severity and the area of adhesions were significantly reduced by LK (P=0.040 and P=0.009, respectively). Loosening and detachment of adhesion band between cecum and abdominal wall was observed in LK treated rats while tight attachment at adhesion site was observed in control. The peritoneal thickness of injured area in the LK treated rats was decreased in dose-dependent manner. The number of migrating cells in LK treated group was significantly low compared with that in control groups (P<0.01). Conclusion: LK prevents peritoneal thickening and reduces tissue adhesion by enhancing fibrinolytic activity and attenuating fibroblast invasion. Therefore, LK is a promising anti-adhesive material to prevent postoperative tissue adhesion.

Reliability and Validity of the Beck Depression Inventory-II among Korean Adolescents

EunHo Lee,SooJi Lee,SoonTaeg Hwang,SangHwang Hong,JiHae Kim 대한신경정신의학회 2017 PSYCHIATRY INVESTIGATION Vol.14 No.1

Objective-The Beck Depression Inventory-II (BDI-II) is one of the most popular scales for evaluating the severity of depression in adolescents as well as adults. The prevalence of depression increases during adolescence, and it has shown a rapid increase with occurrence at an earlier age and a tendency to continue into adulthood. Data from an adolescent nonclinical sample provides us more information related to depressive symptoms as potential risk factors. The current study was designed to two objectives: 1) to analyze the reliability and validity the BDI-II among Korean adolescents and 2) to evaluate the factorial structure in a Korean nonclinical adolescent sample. Methods-The participants included 1072 adolescent boys and girls. We assessed the internal consistency, corrected item-total correlation, and the convergent validity of the BDI-II. We also performed confirmatory factor analyses to determine the internal structure of the BDI-II for Korean adolescents using Mplus 6.1. Results-The Cronbach’s alpha for the BDI-II total score was 0.89. The correlation between the BDI-II and the PHQ-9 was strong (r=0.75), and anxiety-related measures were 0.68 and 0.71, which were also in the high range. Among the five different factor structures, the modified three-factor model demonstrated the best overall fit. Conclusion-The BDI-II is a reliable tool for measuring the severity of depressive symptoms in Korean adolescents. Therefore, the findings can provide basic information for examining the prevalence rate, intervention strategies for depression in adolescents.

Evaluation of Toxicity for Commercial Red Mud Pellets Using Pseudokirchneriella subcapitata and Daphnia magna

Saeromi Lee,Chang Hyuk Ahn,Jae Roh Park,Sooji Lee,Inju Lee,Jin Chul Joo 응용생태공학회 2015 Ecology and resilient infrastructure Vol.2 No.4

The toxicity of red mud (RM) pellets for water purification was evaluated using Pseudokirchneriella subcapitata and Daphnia magna in a lab-scale experiment. According to the algal growth inhibition test, both specific growth rates and relative growth rates of P. subcapitata decreased, and the growth inhibition rates increased (R 2=0.97, p<0.001) as the concentration of RM pellets in the aqueous solution increased (>1.6 g/L). Also, based on the acute toxicity evaluation test on D. magna, toxic unit (TU) values ranged between 0.00 and 2.83, and increased with an increase in the concentration of RM pellets in the aqueous solution. A correlation analysis indicated that the pH of RM pellets was statistically correlated with TU values (R 2=0.77, p=0.02). The environmental implication from this study is that the concentration of RM pellets in an aqueous solution needs to be lower than 4.4 g/L to keep the maximum permissible TU value less than 1.0.

Clinical Significance of Bronchodilator Response (BD) in COPD

( Yejin Lee ),( Youlim Kim ),( Hyun Lee ),( Soojie Chung ),( Hayoung Choi ),( Chang Youl Lee ),( Yong Il Hwang ),( Seung Hun Jang ),( Yong Bum Park ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.-

Background Clinical significance and characteristics of COPD patients with bronchodilator response (BD) has not been characterized rigorously in previous researches. We aim to investigate whether there is difference with characteristics and exacerbation rate in patients with BD among COPD patients. Methods We performed retrospective comparative analysis of clinical characteristics, moderate and severe exacerbation rate in COPD patients who completed the lung function at least three times with either BD positive or negative. In total, 319 patients (33.5% with BD positive and 66.5% with BD negative) were included in the analysis. Results Compared with BD negative patients, there was no significant difference with age, sex, smoking status, and comorbidity in those with BD positive. Mean FEV1(L) (1.5 ± 0.5) were significantly lower in patients with BD positive than those (1.7 ± 0.7) of BD negative patients. There was no difference with eosinophil count, medication possesion ratio, and inhaler choice except long acting beta agonist. Both moderate and severe exacerbation rate ( 0.05 and 0.06, respectively) and of BD (+) was almost 50% lower than those (0.11 and 0.11, respectively) in the BD negative patients. Conclusion We found that both moderate and severe exacerbation rate was significantly lower in patients with BD positive COPD patients compared with those in BD negative COPD patients.

화장품 분석 서비스 어플 UX 평가

이재인(Jaein Lee),최수지(Sooji Choi),이유경(Yookyung Lee) 대한인간공학회 2020 대한인간공학회 학술대회논문집 Vol.2020 No.10

A novel splicing variant of b -TrCP1, a binding protein of b-catenin, in epithelial ovarian cancer

( Yeo-jin Lee ),( Bo-ra Lee ),( Ji Hye Kim ),( Dhan-ah Chae ),( Jin-ah Oh ),( Sooji Ham ),( Hyun-jin Choi ),( Eun-ju Lee ) 대한산부인과학회 2020 대한산부인과학회 학술대회 Vol.106 No.-

Objective: Three splicing variants of b-TrCP1, b-TrCP1-variants 1, 2, and 3, have been found in human cells. We sought to identify a novel variant, b-TrCP-variant 4, whose full-sequence has never been cloned, from ovarian cancer cells and evaluate its regulatory function on b -catenin. Methods: Normal and cancerous ovarian tissues were obtained and snap frozen in liquid nitrogen. Total RNA was isolated. Approximately 0.5-1.0 g of total RNA was reverse transcribed into cDNA using, real-time PCR. To quantitatively determine the mRNA concentration of the variants, real-time PCR was performed. Co-immunoprecipitation assay and b-galactosidase assay were done. The membrane bound proteins were developed by Western blot analysis. The expression and localization of the proteins was observed under by immunofluorescence staining and confocal microscopy. Luciferase activity was assessed using a dual-luciferase reporter assay kit. Results: A novel b-TrCP1-variant 4 harbors exon II instead of exon III of variant 1, with no change in the open reading frame. The expression of b-TrCP1-variant 4 is abundantly expressed in cancerous ovarian tissues rather than variant 1 and 2. Similar to variants 1 and 2, b-TrCP1-variant 4 directly interacts with b-catenin, one of the substrates of SCF-TrCP E3 ubiquitin ligase and down-regulates the transcriptional activity and protein expression of b-catenin with a significantly weaker effect than that by variants 1 and 2. However, the co-expression of b-TrCP1-variant 4 with variant 1 in same proportion has no effect, whereas other combinations effectively downregulate the activity of b-catenin, indicating that the heterodimer of variants 1 and 4 has no function. Conclusion: B-TrCP1-variant 4 could play a critical role in SCF-TrCP E3 ligase-mediated ubiquitination by acting as a negative regulator of -TrCP1-variant 1.

Postoperative infection after primary debulking surgery was associated with poor overall survival of the patients with advanced serous ovarian cancer

( Yeo-jin Lee ),( Ji Hye Kim ),( Dhan-ah Chae ),( Jin-ah Oh ),( Sooji Ham ),( Jiyeon Koo ),( Soo-gui Shin ),( Eun-ju Lee ) 대한산부인과학회 2021 대한산부인과학회 학술대회 Vol.107 No.-