생쥐에 있어서 單一害球가 分離된 受精卵의 移植에 의한 새끼生産에 관한 硏究

崔有林,全益秀,金宣宜,崔光洙,朴修奉,辛明宰 慶北大學校農業科學技術硏究所 1991 慶北大農學誌 Vol.9 No.-

本 연구는 생쥐의 4細胞期胚에서 하나의 割球를 뽑아내는 새로운 割球分離技術인 biopsy에 대한 효율성과 分離된 受精卵과 分離한 割球의 생존성 및 새끼생산율을 검토한 結果이다. 그 結果를 要約하면 다음과 같다. 1. 4細胞期胚에서 分離한 單一割球와 4細胞期胚를 M2 배양액에 배양한 結果, 單一割球는 82.6%가 영양배엽을 형성한 割球로 發生하였고 4細胞期胚로 89.5%가 胚盤胞期胚로 發生하였다. 2. 4細胞期胚를 biopsy하여 하나의 割球가 分離된 受精卵과 대조구인 4細胞期胚를 M2배양액에서 배양한 결과 각각 83.3%와 90.4%가 胚盤細胞胚로 發生하였다. 3. Biopsy하여 4細胞期胚에서 分離한 單一割球와 대조구인 4細胞期胚에서 4개의 割球로 분리한 單一割球를 M2 배양액에서 배양한 결과 각각 80.8%와 83.3%가 영양배엽을 형성한 割球로 發生하였다. 4. 4細胞期胚에서 하나의 割球를 뽑아낸 受精卵과 대조구인 4細胞期胚를 수란생쥐에 이식한 결과 각각 36.0%와 48.6%의 새끼쥐 생산率을 얻었다. The study was carried out to investigate the viability and the offspring production rate of single blastomeres and biopsied 4-cell mouse embryos and, also to examine the efficiency of biopsy. The results obtained are summerised as follows : 1. The separated blastomeres from 4-cell embryos and intact 4-cell embryos which were cultured in Medium 2 were developed to trophoblastic vesicle and blastocyst embryo by 82.6% and 89.5%, respectively. 2. The biopsied embryos from 4-cell embryos and intact 4-cell embryos which cultured in Medium 2 were developed to blastocyst embryo by 83.3% and 90.4%, respectively. 3. The biospied blastomeres and the separated blastomeres from 4-cell embryos which were cultured in Medium 2 were developed to trophoblastic vesicle by 80.8% and 83.3%, respectively. 4. The biopsied embryos from 4-cell embryos and intact 4-cell embryos were transferred to recipients, and the offspring rate was 36% and 48.6%, respectively.

자연산 점몰개, Squalidus multimaculatus Hosoya et Jeon의 새인두흡충, Clinostomum complanatum (Rudolphi, 1814) 감염

박명애 ( Myoung Ae Park ),서정수 ( Jung Soo Seo ),정승희 ( Sung Hee Jung ),최희정 ( Hee Jung Choi ),전은지 ( Eun Ji Jeon ),지보영 ( Bo Young Jee ),이완옥 ( Wan-ok Lee ),우승호 ( Sung Ho Woo ),이은혜 ( Eun Hye Lee ) 한국어병학회 2011 한국어병학회지 Vol.24 No.2

경상북도 영덕군의 하천에 서식하는 점몰개 spotted barbel gudgeon (Squalidus multimaculatus Hosoya et Jeon)의 근육에서 새인두흡충 Clinostomum complanatum (Rudolphi, 1814)의 피낭유충이 발견되었다. 감염된 점몰개는 "yellow grub"인 기생충의 피낭유충으로 인해 표면이 울퉁불퉁하게 보였으며, 수면위에 힘없이 유영하였고 일부 폐사한 개체도 발견되었다. 분리된 탈낭유충의 형태는 혓바닥 모양이었으며, 크기는 2.85~5.89 mm×0.8~1.99 mm였다. 어체당 1~98 개의 피낭유충을 분리하였으며 평균 감염수는 25개로 나타났다. 특히, 성어크기인 평균 7 cm 정도의 점몰개는 100% 감염되어 있었다. 본 연구는 한국의 자연수계에 서식하는 어류에서 C. complanatum 의해 질병이 발생하고 폐사가 일어난 첫 보고이다. The metacercariae of Clinostomum complanatum (Rudolphi, 1814) were isolated in the whole body muscle of wild freshwater fish, spotted barbel gudgeon (Squalidus multimaculatus Hosoya et Jeon) from the local river in Yeongdeok, Gyeongsangbuk-do, Korea. The infected fish with bumpy body surface by "yellow grub" showed erratic swimming behavior and some fish were died. The isolated excysted metacercariae were tongue shaped and 2.85~5.89 mm × 0.8~1.99 mm in size. 1~98 metacercariae were isolated in individual fish and mean infection intensity was 25. All examined adult spotted barbel gudgeon sizing 7 cm in body length were infected. This is the first report of disease outbreak and mortality caused by C. complanatum infection in wild freshwater fish in Korea.

유육종증 1례

전수영,정운태,황성보,류종철,최수전,고일향 인제대학교 1993 仁濟醫學 Vol.14 No.4

저자들은 폐결핵 추정하에 치료를 받았던 Sarcoidosis 환자 1례를 경험했기에 문헌적 고찰과 함께 보고하는 바이다. Sarcoidosis is a granulomatous disease of unknown etiology In which pulmonary manifestations typically predominate with protean extrapulmonary and systemic manifestations. In sarcoidosis, pulmonary symptoms include dyspnea on exertion, non-productive cough and wheezing. Typical radiologic finding is a diffuse infiltration with or without bilateral symmetric hilar lymphadenopathy. Sarcoidosis should be differentiated with pulmonary tuberculosis because of its clincal and radiological slmilarity in Korea, an endemic area of pulmonary tuberculosis. We report a case of sarcoidosis patient whose symptoms were not improved by treatment with anti-tuberculous medication, but by steroid therapy following the diagnosis of sarcoidosis made by biopsy.

學校周邊 道路交通 騷音과 學生 聽感反應과의 相關關係 硏究

김종수,최동훈,박진식,김석택,김장호,서정민 동아대학교 환경문제연구소 1997 硏究報告 Vol.20 No.2

For my thesis I have analyzed the differences between the traffic noise level surrounding the schools and the perceived level of noise. I have used the ways of measurement and questionnaires to support my study. According to the analysis, the measured noise level was higher than the expected noise level. The equivalent noise level was proven to be higher when the windows of the classrooms were open. The noise level was also higher on the third floor than the first floor. The correlation between the measured noise level and the perceived level of noise was tested in each school. The school which had a higher expected noise level showed a high perceived level of noise. The measured noise level and the perceived level of noise became larger on the higher floors. Through the questionnaires, the traffic noises were proven to be the largest noise-making source surrounding the schools. Most of the students replied on the questionnaires that they have been consistently bothered by the noise. It interfered with their studies and concentration. Also, the sound of their voices became louder because of the noise around their schools. Therefore, I have come to the conclusion that the traffic noises that surround the schools give the students negative influences.

전립선 기질세포의 증식과 COX-2 발현에 대한 프로게스테론의 영향

정수련,김성한,최이화,박지은,전은미,강영진,이광윤,최형철 영남대학교 의과대학 2006 Yeungnam University Journal of Medicine Vol.23 No.1

전립선비대증은 노인 남성에서 흔히 유발되는 질환이며, 노화가 진행될 수록 빈도가 높아지는 특징을 가진다. 이 질환의 원인은 전립선기질세표의 과도한 증식으로 유발된다고 알려져 있지만 그 자세한 기전에 대해서는 잘 알려져 있지 않다. 전립선비대증에서 progesterone 수용체 양성 세포가 다른 전립선 종양에 비해서 많고, progesterone은 testosterone에서 DHT로 전환되는 것을 감소시키는 역할을 가진다고 알려졋다. 또한 남성 전립선 평활근의 과증식에 의한 질환이므로 평활근 세포의 증식과 관련성이 있다고 보고된 COX-2의 전립선비대증에 대한 영향에 대한 연구가 필요하다. 전립선 기질세포에 progesterone을 3일간 투여하여 배양한 경우 기질세포 증식은 차이가 없었다. Progesterone을 단독 또는 DHT와 같이 투여한 기질세포에서 남성호르몬 수용체 mRNA 발현은 비처리군과 비교하여 유의한 차이가 없었다. 또한 progesterone과 DHT 동시 투여에 의한 COX-2 mRNA 발현에도 차이가 없었다. 그러나 progesterone에 의한 남성 호르몬 수용체와 COX-2 단백 발현에서는 대조군과 비교하여 유의하게 감소시켰다. 이상의 결과는 progesterone은 남성호르몬 수용체에 대해 전사 후 반응 (post-transcriptional response)에 효과를 나타내어 남성호르몬 수용체 발현을 감소시키는 작용은 가지며, COX-2 발현 억제효과를 나타내므로 전립선비대증의 치료에 이용될 수 있을 것으로 사료된다. Background: Benign prostatic hyperplasia (BPH) is the most common benign tumor in older men; the etiology of this disease remains poorly understood. Testosterone and dihydrotestosterone (DHT) both act as androgen via a single androgen receptor. Testosterone is converted to DHT by 5α-reductase in prostatic stromal cells. Progesterone has been reported to inhibit DHT conversion; howevwe, its effect on prostatic stromal cells remains to be elucidated. Materials and Methods: In this experiment, we investigated the effect of progesterone on androgen receptor expression induced by DHT. We also tested the effect of progesterone on cyclooxygenase-2 (COX-2) expression, as well as prostate stromal cell proliferation using the cell count kit-8. Results: Progesterone did not cause an increase of prostate stromal cell proliferation. The mRNA expression of the androgen receptor and COX-2 were not changed by progesterone; the expressions of androgen receptor and COX-2 proteins were decreased by progesterone in prostate stromal cells. Conclusion: These results suggest that in prostate stromal cells, progesterone decreases androgen receptor protein expression, which results in decrement of COX-2 protein expression. This effect might be mediated by post-transcriptional regulation.

Clozapine 투여로 유발된 무과립구증 1례

이기철,정홍경,이정호,최영민,전성일,최수전 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

저자들은 clozapine 투여 29일이 경과한 시점에서 무과립구증을 보인 20세 여자환자 1례를 치험하였기에 문헌고찰과 함께 보고하였다. 무과립구증은 무균처치와 항생제투여 및 G-CSF와 GM-CSF를 투여하여 무과립구증 상태는 8일만에 회복되었다. Clozapine에 의해 유발된 무과립구증의 원인은 아직까지 확실치 않다. 현재까지 알려진 원인으로는 MHC(major histocompatibility complex region)의 우성인자가 원인이라는 제안과 대사산물이 자유기근으로서 골수세포를 억제한다는 설이 제안되고 있다. 저자들은 clozapine을 투여함에 있어서 환자의 선정, 위험인자들의 고려 및 철저한 CPMS 관리를 제안하고, 일단 무과립구증이 발생하면 즉각적으로 내과적인 도움을 받을 것을 권장한다. We report a 20-year-old female patient who developed clozapine-induced agranulocytosis on the 29th day of clozapine treatment. She recovered from the agranulocytosis on the 8th day of progression after treatment with G-CSF, GM-CSF, antibiotics and associated aseptic procedures. The cause of clozapine-induced agranulocytosis is still un known and it is proposed that the dominant gene of major histocompatibility complex region and the reactive metabolites which suppress the myeloid system may be responsible. We recommend that careful attentions such as the selection of patient, evaluation of the risk factors, and the thorough control of CPMS should be paid. If the agranulocytosis happens, it is helpful to consult to the department of medicine immediately.

국내에서 유행하는 HIV의 전파 경로에 따른 Subtype 분포

이주실,남정구,김성순,강춘,최병선,김옥진,박미선,성봉모,서순덕,전수경,변승옥,신영오,조해월 대한감염학회 2001 감염 Vol.33 No.5

Background : Previous data have been reported that subtype B is prevalent in South Korea, but neither the extent nor the proportion of subtypes could be evaluated. This study was designed to analyze the distribution of HIV-1 subtypes, temporal instructions and transmission dynamics between epidemiological groups. Methods : 1,280 Koreans had been diagnosed as HIV seropositive during the period 1985 to 2000. Among them, 134 individuals were selected for this molecular epidemiological study. 134 DNAs were isolated from uncultured or cultured peripheral blood mononuclear cells. V3-V5 (0.7 kb) fragment of HIV-1 env gene was amplified by nested polymerase chain reaction and was sequenced. Results : HIV-1 isolates from thirty-seven homosexuals were all subtype B (100%). On the other hand, 66 isolates from 94 heterosexuals were subtype B (70%) and 28 were non B subtypes (30% : 13 A, 4 C, 2 D, 8 E , 1 G). Only subtype B strains were isolated from 73 males who were infected with HIV inside Korea while 16 B and 20 non B subtype strains were isolated from 36 males who were HIV infected outside of Korea. However, B and non B strains were isolated half and half from females who were infected inside Korea except one. Conclusion : The HIV-1 subtype B strains are prevalent in Korea from the early HIV infection until present in both homo and heterosexuals. Non B strains have been transmitted from men who were infected outside Korea to their spouses and casual partners. So, we need further study to monitor subtype B and non B HIV transmission in epidemiological groups of Korea, (Korean J Infect Dis 33:311∼318, 2001)

Intermittent hypoxia in obstructive sleep apnea : protective or detrimental for the heart ?

( Soo Jeon Choi ) 대한결핵 및 호흡기학회 2019 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.127 No.-

Chemical Modification of Bovine Brain Succinic Semialdehyde Reductase by Diethylpyrocarbonate

Choi, Soo Young,Cho, Sung Woo,Lee, Kil Soo,Lee, Byung Ryong,Choi, Eun A,Bahn, Jae Hoon,Jeon, Seong Gyu,Choi, Kyung Soon,Yoon, Byung Hak,Ahn, Yoon Kyung 생화학분자생물학회 2000 BMB Reports Vol.32 No.3

The NADPH-dependent succinic semialdehyde reductase is one of the key enzymes in the brain GABA shunt, and it catalyzes the formation of the neuromodulator y-hydroxybutyrate from succinic semialdehyde. This enzyme was inactivated by diethylpyrocarbonate (DEP) with the second-order rate constant of 1.1 × 10³ M^(-1)min^(-1) at pH 7.0, 25℃, showing a concomitant increase in absorbance at 242 nm due to the formation of N-carbethoxyhistidyl derivatives. Complete inactivation of succinic semialdehyde reductase required the modification of five histidyl residues per molecule of enzyme. However, only one residue was calculated to be essential for enzyme activity by a statistical analysis of the residual enzyme activity. The inactivation of the enzyme by DEP was prevented by preincubation of the enzyme with the coenzyme NADPH but not with the substrate succinic semialdehyde. These results suggest that an essential histidyl residue involved in the catalytic activity is located at or near the coenzyme binding site of the brain succinic semialdehyde reductase.

Synthesis of nanostructured Li2MnSiO4/C using a microwave assisted sol–gel process with water as a base solvent

Choi, Jeon-Jin,Kim, Soo,Im, Won-Bin,Chang, Wonyoung,Cho, Byung-Won,Kim, Jong Hak,Choi, Hee-Lack,Chung, Kyung Yoon Springer-Verlag 2013 JOURNAL OF ELECTROCERAMICS Vol.31 No.1