Effect of ER signal peptides (KDEL) on expression and function of monoclonal antibody in plant

Da-Young Park,Eun-Yi Oh,Zhe Lu,Yangkang So,Mi-Hyun Ahn,Mira Song,Kyung-Jin Lee,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current animal expression systems. In this study, the expression level of mAbP SO57 between with and without Lys-Asp-Glu-Leu (KDEL) was compared in transgenic plant. PCR and PT-PCR analyse showed stable gene insertion transcription of heavy and light chain genes of mAbP SO57 with or without KDEL in plant, respectively. We validated expression of mAbP SO57 by western blot. Western blot showed the significantly higher expression level of mAbP SO57 with KDEL compared to without KDEL. Flow cytometry (FACS) analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb (mAbM) evidenced similar binding activity to the FcγRI receptor (CD64). High performance liquid chromatography (HPLC) analysed, glycosylation patterns of mAbP SO57 with or without KDEL. The mAbP SO57 with KDEL had glycan profile with both oligomannose type (47.6%) and golgi type (52.4%), while the mAbP SO57 without KDEL had only golgi type (100%) glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest the potential of mAbP SO57 for rabies immunotherapy, regardless of plant specific glycan structures.

은행나무의 잎끝마름병에 미치는 SO2 의 영향

이두형,배공영,박소홍,강연지 한국환경농학회 1999 한국환경농학회지 Vol.18 No.2

대기오염과 병충해발생과의 관계를 규명하고자 은행나무에 SO₂가스를 처리한 후, 잎끝마름병균을 접종하여 식물의 생리·생화학적인 변화를 조사하였다. 0.1㎕/ℓ의 SO₂가스를 하루 8시간씩 7일간 은행나무에 처리한 결과 Chlorosis등의 가시피해는 나타나지 않았으나, 광합성이 대조구에 비해 40%가량 감소되고, CO₂이용효율과 수분이용효율이 감소되는 현상을 보였다. 가스처리 후 병원균을 상처접종시키고 42일 뒤 SI와 SFI의 발병도를 조사한 결과 SI가 SFI에 비해 3배 가량 빠르게 병이 진전되어 있었다. 병원균의 먹이가 되는 당의 함량은 대조구, SFI, SI간에 유의성을 보일 만큼의 차이는 보이지 않았으나, 광합성은 SI가 SFI에 비해 11%가량 저하되었다. 광합성이 저하되었음에도 불구하고 당의 함량이 유지되었으므로 탄소 고정관계를 알아본 결과 CO₂ 이용효율이 저하되지 않았음을 알 수 있었다. 이와 함께 수분이용효율이 SI에서 SFI에 비해 13.2% 증가되었다. 이상의 결과를 종합해 보면, 이온유출량 등으로 보아 SO₂가스처리로 인해 식물이 연약해져 병원균에 대한 저항력이 낮아졌고, 수분이용효율의 증가로 인해 탄소 고정이 활발하게 이루어짐으로써 당이 원활히 합성되는 한편 내부적인 변화로 인해 합성된 당의 전류가 억제되어 잎에 당이 축적되었기 때문에 SI의 발병도가 훨씬 높았던 것으로 생각된다. To examine the relationship between air pollution and occurrence of disease in plants, we investigated the alteration of physiology and biochemistry of Ginkgo biloba by inoculating with Coniothyrium sp. and fumigating with 0.1㎕/ℓSO_(-2) Visual damage did not appear but photosynthesis, CO₂use efficiency(CUE) and water use efficiency(WUE) were reduced when G. biloba was exposed to SO₂for 7days (8 hours a day). When inoculated with Coniothyrium sp., the seventies of damage in G. biloba showed a threefold difference between SO₂-treatment (SI) and SO₂-free treatment (SFI) at day 42 from initial inoculation. Little difference was observed in sugar contents that may be used pathogens feed, among control, SFI and SI. In spite of the reduction in photosynthetic rate, sugar contents and CUE were maintained. WUE was enhanced 13% more at SI than SFI. The CO₂fixation boosted because of enhanced WUE, and thus sugar synthesis was not affected. In addition, sugar transport seems to be retarded for some internal alteration. Consequently, the severity of SI was more serious than that of SFI because Coniothyrium sp. easily invades into the physical texture of G. biloba weakened by SO₂fumigation and because sugar was accumulated in leaves of G. biloba.

林地利用에 따른 溪流水의 水質變化

具瀟瑛,鄭源玉,金弘洙,朴晋源,麻鎬燮 경상대학교 농과대학 부속연습림 2001 演習林硏究報告 Vol.- No.11

Table 2. Evaluation of stream water quality in three stands by Drinking water quality standard <원문참조> 임지이용에 따른 수질변화 및 오염정도를 구명하여 향후 비점오염원 관리를 위한 기초자료를 제공하고자 경남 진주시 정평리에 위치한 3개 임분(소나무, 밤나무Ⅰ, 반나무Ⅱ임분)에서 2000년 7월부터 9월까지 계류수의 수질변화를 분석하여 얻은 결과는 다음과 같다. 1. 세 임분의 계류수 pH는 소나무 임분(pH6.59)>밤나무 Ⅱ임분(ph 6.53)>밤나무 Ⅰ임분(pH 6.47)계류수 순으로 나타났다. 2. 전기전도도는 밤나무 Ⅰ임분>소나무 임분>밤나무 Ⅱ임분 계류수의 순으로 높게 나타났다. 3. 양이온은 세 임분의 계류수 모두 Ca^2+, Na^+, Mg^2+, K^+, NH_^+의 순으로 높게 나타났다. 4. 음이온은 소나무 임분과 밤나무 Ⅱ임분 계류수에서는 SO_4^2, Cl, NO_3의 순으로 높게 나타났고, 밤나무 Ⅰ임분 계류수에서는 NO_3, SO_4^2, Cl 의 순으로 높게 나타나 차이를 보였다. 5. 소나무, 밤나무 Ⅱ임분 계류수는 pH, NH_4+, NO_3, Cl, SO_4^2이 먹는 물 수질기준 범위내에 있었다. 그러나 밤나무 Ⅰ임분 계류수에서 5항목 중 NO_3는 먹는 물 수질기준을 넘어서는 범위에 있어 임지이용에 따른 하류수질의 오염이 우려되었다. 따라서 이러한 상류지역에서 오염원 차단을 위한 수질정화시설 등의 대책이 마련되어야 할 것으로 사료된다. This study was carried out to clarify the change characteristics of stream water quality by land-use of forest from July to September, 2000 in three stands(Pinus densiflora, Castanea crenata(Ⅰ), Castanea crenata(Ⅱ) stand) of Jeongpyeong-ri, Jinju-si, Gyeongnam. The mean pH of rainfall results in acid rain of 5.3. The pH of stream water in three stands was hugh in order of Pinus densiflora (pH 6.59), Castanea crenata(Ⅱ)(pH 6.53) and Castanea crenata(Ⅰ) stand(pH 6.47). The electrical conductivity of stream water was high in order of Castanea crenata(Ⅰ), Pinus densiflora and Castanea crenata(Ⅱ) stand. Cations of three stand in stream water high in order of Ca^2+, Na^+, Mg^2+, K^+ and NH_4^+. But anions of stream water in Pinus densiflora stand and Castanea crenata(Ⅱ) stand were high in order of SO_4^2, Cl and NO_3. In Castanea crenata(Ⅰ) stand cations of stream water were high in order of NO_3, SO_^2 and Cl. The level of pH, NH_4^+, NO_3, Cl and SO_4^2 of stream water in Pinus densiflora stand and Castanea crenata(Ⅱ) stand reached within the level of domestic use standard for drinking water But the level of NO_3 of stream water in Castanea crenata(Ⅰ) stand was higher than that of domestic use standard. Therefore, non-point sources like forest watersheds which are fertilizer application lands should be taken to the appropriate mitigation measures.

Effect of ER signal peptides (KDEL) on expression and function of anti-virus monoclonal antibody in plant

Da-Young Park,Yangkang So,Kyung-Jin Lee,Zhe Lu,Eun-Yi Oh,Kwang-Wok Min,Seul-Ki Lee,Jung-Hwan Lee,Mi-Hyun Ahn,Mira Song,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering has led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current antibody expression systems. In this study, the expression levels of mAbP SO57 with or without ER-signal peptides (Lys-Asp-Gly-Leu;KDEL) in transgenic tobacco plants were analysed in transgenic plant. PCR and Reverse Transcription-PCR analyses showed existence of heavy and light chain genes of mAb with or without KEDL and their transcription in plant, respectively. Western blot showed that the expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb have similar binding activity to the FcγRI receptor. High-performance liquid chromatography analysis showed that the mAbP SO57 with KDEL had glycan profile with both oligomannose and golgi type, whereas the mAbP SO57 without KDEL had only golgi type glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest that the potential of mAbP SO57 for rabies immunotherapy is regardless of plant specific glycan structures.

Verbal Noun Constructions Revisited

So-Young Park 한국생성문법학회 2010 생성문법연구 Vol.20 No.2

This paper investigates a pattern of verbal noun constructions in which a verbal noun is accusative marked and its theme argument is also accusative marked. Based on the two arguments capitalizing on indeterminate pronoun licensing and passive formation, this paper proposes that verbal nouns can take two distinct types of complementation structures: a nominalized structure embedding a vP (with a non restructuring verb hata ‘do’) or a nominalized structure embedding a VP (with the restructuring verb hata). The verb hata turns out to be a lexical restructuring verb, which has an option to take restructuring complementation. This paper argues against the complex predicate approach to verbal noun constructions, favoring the VP-complementation approach which holds that a verbal noun, without the help of the so-called light verb hata, theta-marks its accusative-marked object argument.

The Role of Human and Definite Features in Korean Pluralization

So-Young Park 한국생성문법학회 2020 생성문법연구 Vol.30 No.2

This paper addresses a specific context where the so-called plural marking - tul in Korean is obligatory for plural denotation, sharply contrasting with the widely known generalization that in classifier languages, plural morphology is merely optional. A human noun in Korean requires a number specification by -tul, when it denotes plural individuals in an anaphoric definite context, whereas a non-human noun does not require it under the same condition. This contrast will be explained by the peculiarity of human nouns that can take null classifiers, distinct from other nouns in Korean. Human nouns, being associated with null classifiers, behave as count nouns and require explicit number specifications by -tul when full DP structures are supposed to project. Non-human nouns, in contrast, are mass nouns without resorting to overt classifiers, and hence do not require number specifications by -tul even when complete DPs are projected. This discussion, in turn, leads to implications on the syntactic status of -tul in Korean, arguing for a head plural analysis, instead of a modifying plural analysis, and further on the DP- vs. NP-language debate of classifier languages.

수학 교수.학습을 위한 P.Ernest 의 사회적 구성주의에 대한 분석적 고찰

박영배,김소영 仁川敎育大學校 科學敎育硏究所 1998 과학교육논총 Vol.10 No.-

Recently, it is a fact that everyone acknowledges constructivism gives a very powerful epistemological foundation for the teaching and learning in Mathematics. But we do not know the details about the constructivist's view. Especially, we don't know the social constructivism, either. So, we investigated social constructivism of P. Ernest to prepare epistemological foundation for the teaching and learning in mathematics. Hence, in the result of investigation of social constructivism, we are become to have two findings such that : First, publication is necessary course to make individual subjective knowledge into objective knowledge. So it is very important that we make a chance for children to announce their thought, freely. Second, a publicated knowledge of children has to be a objective knowledge through their social interactions. And then, it is very important that we engage the children in their social interaction actively to succeed their interacting activities. Consequently, we are become to know that social constructivism can be a epistemological foundation of the teaching and learning in mathematics, and gives a good philosophical view of mathematical knowledge.

한국어 주어일치소에 대한 새로운 접근: 음성형식부 일치 분석

박소영 ( Park So-young ) 서강대학교 언어정보연구소 2021 언어와 정보 사회 Vol.43 No.-

This paper presents a PF-based analysis of the so-called subject agreement forms in Korean such as -si- and -tul. These morphemes exhibit the properties that are not consistent with the predictions of syntactic agreement; their realizations are optional, and their overall distributions cannot be fully captured by any syntactic rules. In addition, semantic interpretations are not substantially affected regardless of their presence or absence. Based on these facts, this paper argues that they should rather be taken as agreement forms at PF, thus inserted by the application of a node-sprouting rule at PF. Their status as subject agreement forms will also be refuted, instead generalizing them as agreeing with their C-commanding antecedents being in the same phase.

Interacelluar reprogramming of expression, glycosylation, and function of plant-derived anti-virus therapeutic monoclonal antibody

Da-Young Park,Kyung-Jin Lee,Yangkang So,Zhe Lu,Seulki Lee,Jung-Hwan Lee,Seung Han Oh,Yeon Soo Han,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2010 한국당과학회 학술대회 Vol.2010 No.1

Plant genetic engineering has led to production of plant-derived mAb (mAbP), which can provides a safe and economically effective alternative to the conventional antibody expression methods. In this study, the expression levels of mAbP SO57 with or without ER-retention peptide extensions signal (Lys-Asp-Glu-Leu; KDEL) in transgenic tobacco plants were analysed in transgenic plant. The expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL regardless of transcription level. mAbP SO57 with KDEL localized surround to the nucleus suggesting that the mAbP with KDEL is localized in ER. The mAbP without KDEL and mAbH had mainly Golgi type glycans, whereas the ER-localized mAbP with KDEL had glycan profile with both oligomannose type (47.6%) and Golgi type (52.4%). The Fc domains of both purified mAbP (with and without KDEL) and human-derived mAb (mAbH) had similar bingding activity to the Fcγ RI receptor (CD64). Both mAbP (with and without KDEL) had a shorter half-life than mAbH. However, both mAbP with and without KDEL was as effective at neutralizing activity of the rabies virus CVS-11 as the mAbH. These results suggest that ER localization of recombinant mAbP by KDEL reprograms glycosylation and enhances production of the functional antivirus therapeutic antibody in the plant.

모리스 블랑쇼로 『맹자(孟子)』 읽기

박소영 ( Park So-young ) 전남대학교 인문학연구소 2017 용봉인문논총 Vol.- No.51

20세기 프랑스 철학자이자 문학가인 모리스 블랑쇼는 언어, 죽음, 문학이 갖는 공통적인 특성으로 익명성, 비인칭, 중성성을 제시한다. 이러한 사유는 탈주체성을 확립하기 위한 근거로 작용하며 이른바 ‘바깥’에서만 존재하는 것이다. 기존의 공개적 정치 참여를 주장했던 블랑쇼는 제2차 세계대전을 경험하면서 우파 진영의 파시즘적 요소들, 전쟁 중 만나게 된 바타유와의 친교를 통해 공동체 사상에 관심을 갖게 된다. 이는 후기 블랑쇼의 정치사상을 변화시키는 계기가 된다. 블랑쇼는 경제 문제 만을 다루는 현실을 비판하고 모든 정치 이론이 근본적인 공동체 윤리적 기초를 반성하는데 목적을 두어야 한다고 말한다. 비단 서양뿐만 아니라, 문학과 정치의 만남은 동아시아 유교 사상의 전통이자 근원을 이루는 키워드다. 맹자(孟子) 또한 사상가이자 정치가, 철학가, 그리고 문학가로서 언어와 글쓰기, 문학, 그리고 공동체에 대한 관심을 『맹자』를 통해 지금의 우리에게 보여주고 있다. 본고는 맹자와 블랑쇼의 논의를 통해 ‘우리’라는 공동체의 의미를 밝히는데 목적을 둔다. 이 공동체는 맹자와 블랑쇼의 이론에 있어 가장 중요한 핵심으로 제시되는 바, 각각의 동이점에 주목하여 어떠한 의의를 갖는가에 대한 고찰을 하고자 한다. 나아가 맹자가 생각하는 문학에 대한 개념과 블랑쇼가 생각하는 문학, 글쓰기 행위에 대한 입장을 확인하는 동시에 결과적으로 각자 어떠한 문학관과 사상을 견지하고 있었는지 살펴보는 계기가 될 것이다. 이러한 논의는맹자와 블랑쇼의 이론과의 만남을 통한 동서양 사상의 결합이라는 새로운 관점을 제시하는 데 의의가 있다. French philosopher Maurice Blanshaw, a 20th century French philosopher and writer, suggests anonymity, inhumanity, and neutrality with a common characteristic of languages, death and literature. These reasons act as a basis for establishing detailability and exist only in the so-called ‘outside’. Blanshaw, who advocated the previous public participation, is concerned with the idea of community thinking, through the experiences of the rightist camp and the friendship of Batayu, who met the rightist faction during the war. This constitutes a change in the political ideology of the late Blanchaw. Blanshaw criticizes the reality of economic affairs and says that all political theories should be aimed at reflecting the fundamental ethical foundation of the underlying community. Not only the Western but also the politics of literature and politics are the traditions and origins of Confucianism in East Asia. Mencius also showed us interest in language, writing, literature, literature, and literature as “Mencius” and literature, and showing us interest in writing. Through the discussions of Mencius and Blanshaw, it aims to clarify the meaning of the community. The Community is regarded as the most important point in the theory of Mencius and Blanshaw, and intends to consider the significance of each point of view. Furthermore, it will be a testament to the literature of the writer and his thoughts on literature and literature, as well as the interpretation of the literature and ideas of literature. Through this discussion, it is important to present a new perspective on the synthesis of Eastern and Western ideas through meeting Mencius and Blanchaw.