자궁경부종양에서 HPVDNAChip^(�) 및 Hybrid Capture Ⅱ^(TM)검사를 이용한 인유두종 바이러스의 검출

여소진,김달수,남계현,심일구,김태희,이해혁,이권해 대한부인종양 콜포스코피학회 2003 Journal of Gynecologic Oncology Vol.14 No.2

목적 : 인유두종 바이러스는 자궁경부암의 의의있는 원인 인자이다. 이 연구의 목적은 HPVDNAChip??검사를 이용하여 정상, 전암병변, 침윤성 경부암의 인유두종 바이러스의 분포를 알아보고 그 결과를 Hybrid CaptureⅡ^(^(TM)) 검사와 비교하였다. 연구 방법 : 149명의 대상환자 중 57명은 병변이 없었고, 59명은 전암 병변이었으며, 33명은 침윤성 경부암이었다. HPVDNAChip??검사와 이 검사처럼 아형 66, 69를 제외한 고위험군(HPV-16/18/31/33/35/45/51/52/56/58/59/68) 인유두종 바이러스를 검사할 수 있는 Hybrid CaptureⅡ^(^(TM))검사를 이용하여 연구하였다. 결과 : 두 검사 모두 인유두종 바이러스를 검사하는 데 유용하였고, 결과가 의의있게 일치하였다(kappa value 0.721, p<0.01). Hybrid CaptureⅡ^(^(TM))검사에서 양성이나 HPVDNAChip??검사에서 음성인 경우는 18명(12.1%)으로 평균 Hybrid CaptureⅡ^(^(TM))값이 120.7±233.0 (mean±SD)이였고, 두 검사 모두 양성인 경우의 Hybrid CaptureⅡ^(^(TM))값은 448.1±732.8 (mean±SD)이였다. Hybrid CaptureⅡ^(^(TM))검사의 민감도는 94.6%이고, 특이도는 78.9%였고 HPVDNAChip??검사의 민감도는 83.7%이고, 특이도는 89.5%였다. HPVDNAChip??검사는 모두 15가지의 고위험군의 인유두종 바이러스 아형을 알아낼 수 있으며 아형 16이 전암병변(28.8%, 15/59)과 침윤성 경부암(48.5%, 19/33)에서 가장 흔한 아형이었으며 그 다음으로 흔한 아형은 58이었다. 결론 : HPVDNAChip??검사는 Hybrid CaptureⅡ^(^(TM))검사처럼 인유두종 바이러스 검사에 매우 민감하고 유용한 검사이다. HPVDNAChip??검사는 Hybrid CaptureⅡ^(^(TM))검사와 비교할 때, 인유두종 바이러스의 아형과 중복감염에 대한 정보를 준다. 앞으로 HPVDNAChip??검사의 자궁경부암의 선별검사로서의 가치에 대한 연구가 더 필요하다. Objective : Human Papillomavirus (HPV) is well known as one of the major risk HPVDNAChip?? factors for cervical cancer. The purpose of this study is to know HPV genotype distribution in women with normal cervix, precancerous lesion, and invasive cervical cancer by HPVDNAChip?? test. In addition, the result of HPVDNAChip?? test was compared with Hybrid CaptureⅡ^(TM) system for HPV detection. Methods : One hundred forty nine patients were included in this study. 57 women had normal cervix, 59 women had precancerous lesion, and 33 women had invasive cervical cancer. We tested them with two method for detection of Human Papillomavirus (HPV) by HPVDNAChip?? test and Hybrid CaptureⅡ^(TM) system. Hybrid CaptureⅡ^(TM) test can detect same high-risk HPVs (16/18/31/33/35/39/45/51/52/56/58/59/68) with HPVDNAChip?? test which can detect additional 66 & 69 high risk types of HPV. Results : Both methods for the detection of HPV were useful tests. The correlation between the results of two methods was very significant (kappa value 0.721 [p<0.01]). Positive Hybrid CaptureⅡ^(TM) test and negative HPVDNAChip?? test group were 18 women (12.1%) and average Hybrid CaptureⅡ^(TM) titer value of this group was 120.7±233.0 (mean±SD), positive Hybrid CaptureⅡ^(TM) test and positive HPVDNAChip?? test group were 84 women (54.4%) and average titer was 448.1±732.8. The Hybrid CaptureⅡ^(TM) test showed a sensitivity of 94.6% and a specificity of 78.9% and the HPVDNAChip?? test showed a sensitivity of 83.7% and a specificity of 89.5%. HPVDNAChip?? test detected total 14 genotype of HPV. HPV-16 was 28.8% (15/59) in precancerous lesion and 48.5% (19/33) in invasive cancer, most common in both groups. Next common type HPV-58 was 25.4% (12/59) in precancerous lesion and 9.1% (3/33) in invasive cancer. Conclusion : HPVDNAChip?? test is very sensitive and effective method for detection of Human Papillomavirus (HPV) infection as Hybrid CaptureⅡTMtest. In comparison with Hybrid CaptureⅡ^(TM) test, HPV genotype and multiple HPV infection information can be given by HPVDNAChip?? test. Further study will be needed to know the value of screening of cervical cancer by HPVDNAChip?? test in future.

Maternal antimullerian hormone as a predictor of fetal aneuploidy occurring in an early pregnancy loss

( So Hyun Shim ),( Hyeong In Ha ),( Yong Wook Jung ),( Sung Shin Shim ),( Yeon Kyung Cho ),( Ji Youn Kim ),( Kyoung Jin Lee ),( Dong Hyun Cha ),( Soo Hyun Kim ),( Hee Jin Park ) 대한산부인과학회 2015 Obstetrics & Gynecology Science Vol.58 No.6

Objective The purpose of the study was to examine the relationship between the parameter representing ovarian reserve and the fetal aneuploidy in early spontaneous miscarriage. Methods A multicenter retrospective cohort study was performed in patients who were diagnosed with early pregnancy loss (≤13 gestational weeks) and examined for fetal karyotype at the CHA Gangnam Medical Center, CHA Bundang Medical Center, and CHA Gumi Medical Center between January 2011 and December 2012. Karyotyping was performed by the Genetic Laboratory of the Fertility Center of CHA Gangnam Medical Center. Medical records were reviewed for demographics, karyotype analysis and hormonal assay of ovarian reserve including antimullerian hormone (AMH) and follicle stimulating hormone. Statistical analysis was performed using SPSS software. Results A total 462 patients were included in this study. The mean age of the patients was 35.31±4.12 years and the mean AMH level was 3.88±3.50 ng/mL (n=195). Two hundred eleven conceptuses (45.7%) of patients showed the euploid and 251 (54.3%) showed the aneuploid. There are significant differences in maternal age, AMH and gestational age between fetal euploid and aneuploid groups (34.46±4.35 vs. 36.04±3.78 years, P<0.001; 4.60±3.86 vs. 3.43±3.18 ng/mL, P=0.022; 7.67±1.54 vs. 8.27±1.46 weeks, P<0.001, respectively). Multivariate analysis revealed that low AMH level and early gestational age were maternal age-independent markers for fetal aneuploid (P<0.001 and P=0.045, respectively). Conclusion Low maternal AMH level might be a predicting marker for fetal aneuploid in early pregnancy loss.

Prader-Willi syndrome originated from Uniparental Disomy(UPD) prenatally diagnosed by DNA methylation analysis and methylation specific-multiplex ligation-dependent probe amplification(MS-MLPA)

( So Hyun Shim ),( Dong Hyun Cha ),( Sung Han Shim ),( Se Ra Sung ) 대한산부인과학회 2018 대한산부인과학회 학술대회 Vol.104 No.-

Objective: To discuss the current status of established and ongoing prenatal genetic testing options for Prader-Willi syndrome(PWS), especially if it comes from maternal unipaternal disomy(UPD), including noninvasive prenatal test(NIPT) and more detailed molecular tests. Methods: PWS, ultimately caused by lack of expression of genes from the chromosome 15 contributed by the father, has been found to be responsible for three genetic causes. Maternal UPD occurs in about 25%, while two-thirds occurred by paternally-contributed 15q11-q13 deletions and remaining by imprinting defect. Since the genetic of PWS is complicated, advent modern genetic analysis is needed to help to identify the PWS. Results: The result of Trisomy 15 in NIPT may suggests PWS with maternal UPD, while true trisomy 15 aborted in early gestations. DNA methylation analysis of chromosome 15, the method of amplifying Bi-S-treated DNA with a maternal primer capable of amplifying the methylated SNRPN(small nuclear ribonucleoprotein polypeptide-N) gene and a paternal primer capable of amplifying the unmethylated SNRPN gene, results that only the maternal allele is amplified. The methylation specific-multiplex ligation-dependent probe amplification(MS-MLPA) is a method using a restriction enzyme that can specifically cut only the unmethy DNA using the property that the maternal allele is always methylated and the paternal allele is always unmethylated among the 15 chromosomes. In Normal, the maternal allele is not truncated and the paternal allele is cut off, resulting in a ratio value of 1. SNRPN gene and NDN(necdin) gene on chromosome 15 are not cleaved into enzyme, resulting in a value twice that of normal. These results means that it is an UPD with two maternal alleles. Conclusion: With regard to complicated hereditary disorders such as UPD, accurate diagnosis with identifying precise genetic subtypes of PWS has been made by growing interest of NIPT and combination of detailed molecular genetic analysis following the abnormal NIPT results.

Prenatal chromosomal microarray analysis of fetus with increased nuchal translucency

So Hyun Shim,Dong Hyun Cha 대한의학유전학회 2018 대한의학유전학회지 Vol.15 No.2

Nuchal translucency is an important indicator of an aneuploid fetus in prenatal diagnostics. Previously, only the presence of aneuploid could be conἀrmed by conventional karyotyping of fetuses with thick nuchal translucency. With the development of genetic diagnostic techniques, however, it has been reported that subtle variations not detectable by conventional karyo-typing might occur in cases of pathologic clinical syndrome in euploid fetuses. One of the newer, high-resolution genetic methods in the prenatal setting is chromosomal microarray. The possible association between nuchal translucency thickness with normal karyotype and submicroscopic chromosomal abnormalities detectable by microarray has been studied. How and when to apply microarray in clinical practice, however, is still debated. This article reviews the current studies on the clini-cal application of microarray in cases of increased nuchal translucency with normal karyotype for prenatal diagnosis.

Prenatal chromosomal microarray analysis of fetus with increased nuchal translucency

Shim, So Hyun,Cha, Dong Hyun Korean Society of Medical Genetics and Genomics 2018 대한의학유전학회지 Vol.15 No.2

Nuchal translucency is an important indicator of an aneuploid fetus in prenatal diagnostics. Previously, only the presence of aneuploid could be confirmed by conventional karyotyping of fetuses with thick nuchal translucency. With the development of genetic diagnostic techniques, however, it has been reported that subtle variations not detectable by conventional karyo-typing might occur in cases of pathologic clinical syndrome in euploid fetuses. One of the newer, high-resolution genetic methods in the prenatal setting is chromosomal microarray. The possible association between nuchal translucency thickness with normal karyotype and submicroscopic chromosomal abnormalities detectable by microarray has been studied. How and when to apply microarray in clinical practice, however, is still debated. This article reviews the current studies on the clinical application of microarray in cases of increased nuchal translucency with normal karyotype for prenatal diagnosis.

Experiences and efficacy of noninvasive prenatal test using maternal plasma in single center: 1,591 cases

So Yeon Hong,So Hyun Shim,Hee Jin Park,Sung Shin Shim,Ji Youn Kim,Yeon Kyung Cho,Soo Hyun Kim,Dong Hyun Cha 대한의학유전학회 2020 대한의학유전학회지 Vol.17 No.1

Purpose: The objective of this study was to analyze the results of several noninvasive prenatal tests (NIPTs) from a single cen-ter and conἀrm their efἀcacy and reliability. In addition, we aimed to conἀrm the changes in the number of invasive tests per-formed after introducing NIPT. Materials and Methods: NIPT data from a large single center from March 2014 to November 2018 were analyzed. Karyotyping was confirmed based on chorionic villus sampling, amniocentesis, or postnatal cord/peripheral blood sampling. Data on maternal age, gestational age, fetal fraction, and ultrasonographic results were analyzed. As the secondary outcome, the number of amniocentesis cases before and after the introduction of NIPT was compared. Results: Overall, 1,591 single pregnancy cases that underwent NIPT were enrolled. The mean maternal age was 36.05 (22-45) years. The average gestational age and fetal fraction were 12+1 (9+3 to 27+1) weeks and 10.95% (3.6% to 31.3%), respectively. A total of 1,544 cases (97.0%) were reported to have negative NIPT re-sults and 40 (2.5%) had positive NIPT results. The sensitivity and specificity of the overall abnormalities in NIPT were 96.29% and 99.36%, respectively. The positive predictive value (PPV) and negative predictive value were 72.22% and 99.93% respectively. The mean number of amniocentesis cases were 21.7 per month (21.7±3.9), which significantly decreased from 31.5 per month (31.5±4.8) before conducting NIPT as a screening test. Conclusion: NIPT is currently a useful, powerful, and safe screening test. In particular, trisomy 21 is highly speciἀc due to its high PPV. NIPT can reduce the potential risks of procedure-related miscarriages during invasive testing.

Experiences and Efficacy of non-invasive prenatal test (NIPT) using maternal plasma in single center: 1591 cases

( So Yeon Hong ),( So Hyun Shim ),( Soo Hyun Kim ),( Hee Jin Park ),( Sung Shin Shim ),( Ji Youn Kim ),( Yeon Kyung Cho ),( Kyung Jin Lee ),( Dong Hyun Cha ) 대한산부인과학회 2019 대한산부인과학회 학술대회 Vol.105 No.-

Objective: To analyze the result of a large number of NIPT in single center and confirm the efficacy and reliability. Methods: The data of NIPT in single center from Mar.2014 to Nov.2018 were analyzed. The patients who referred from other hospital due to abnormal result were also included. All of the patient have completed the delivery. Karyotyping confirmation was done by CVS or amniocentesis or postnatal cord blood sampling. We consider normal karyotype if there were no special abnormalities during pregnancy, no abnormal phenotype in neonate after birth. Details on age, gestational age, platform, fetal fraction, sonographic results were analyzed. Results: Total 1591 single pregnancy cases who performed NIPT were enrolled.640cases were individual SNP method, and 951cases were whole genome MPS method. Mean age was 36.1(22-45) years and gestational age, fetal fraction were 12+1(9+3-27+1)wks and 10.9(3.6-31.3)%.1544cases(97.1%) reported as negative NIPT results,40cases(2.5%) reported as positive NIPT results,7 cases(0.4%) were uninformed. In positive group, only 36cases cytogenetic confirmation tests were done whereas 4cases were F/U loss. Confirmed 36cases were following; Among the 19karyotype tests in NIPT trisomy21 positive group, all 19cases revealed as trisomy 21(100%).In 2cases of trisomy18 high risk group, 1case(50%) was confirmed as trisomy18.Trisomy13(n=1) was also confirmed as trisomy13.Among Sex chromosome abnormality group(n=8), 4cases showed discordancy result. Since we used MPS method, other uncommon trisomy;trisomy15(n=2),7(n=1) and 14(n=1) were incidentally detected. In this study, sensitivity and specificity were 96.3% and 99.4%, PPV and NPV were 72.2% and 99.9%,respectively. Conclusion: Clinical review of general cases are introduced based on single center’s results. NIPT is currently a useful, powerful and safe screening test, as the result we can reduce invasive test. In particular, trisomy 21 is highly effective due to its high PPV. In case of trisomy13,18 and SCA, confirmed test is necessarily required.

Risk Factors of Postpartum Depression Among Korean Women: An Analysis Based on the Korean Pregnancy Outcome Study (KPOS)

Shim So Hyun,Lee Su Young,Jung Inkyung,Heo Seok-Jae,Han You Jung,Kwak Dong Wook,Kim Min Hyoung,Park Hee Jin,Chung Jin Hoon,Lim Ji Hyae,Kim Moon Young,Cha Dong Hyun,Shim Sung Shin,Cho Hee Young,Ryu Hyu 대한의학회 2024 Journal of Korean medical science Vol.39 No.3

Background: Postpartum depression (PPD) can negatively affect infant well-being and child development. Although the frequency and risk factors of PPD symptoms might vary depending on the country and culture, there is limited research on these risk factors among Korean women. This study aimed to elucidate the potential risk factors of PPD throughout pregnancy to help improve PPD screening and prevention in Korean women. Methods: The pregnant women at 12 gestational weeks (GW) were enrolled from two obstetric specialized hospitals from March 2013 to November 2017. A questionnaire survey was administered at 12 GW, 24 GW, 36 GW, and 4 weeks postpartum. Depressive symptoms were assessed using the Edinburgh Postnatal Depression Scale, and PPD was defined as a score of ≥ 10. Results: PPD was prevalent in 16.3% (410/2,512) of the participants. Depressive feeling at 12 GW and postpartum factors of stress, relationship with children, depressive feeling, fear, sadness, and neonatal intensive care unit admission of baby were significantly associated with a higher risk of PPD. Meanwhile, high postpartum quality of life and marital satisfaction at postpartum period were significantly associated with a lower risk of PPD. We developed a model for predicting PPD using factors as mentioned above and it had an area under the curve of 0.871. Conclusion: Depressive feeling at 12 GW and postpartum stress, fear, sadness, relationship with children, low quality of life, and low marital satisfaction increased the risk of PPD. A risk model that comprises significant factors can effectively predict PPD and can be helpful for its prevention and appropriate treatment.

과채류 중 Thimetoxam의 잔류 특성

박소현 ( So-hyun Park ),정진욱 ( Jin-wook Jung ),전영환 ( Young-hwan Jeon ),정혜현 ( Hye-hyun Jung ),심재룡 ( Jae-ryong Shim ),배병진 ( Byung-jin Bae ),채석 ( Seok Chai ),박종우 ( Jong-woo Park ),김태화 ( Tae-hwa Kim ) 한국환경농학회 2017 한국환경농학회 학술대회집 Vol.2017 No.-

딸기, 토마토 및 토마토 가공품 중 Thiamethoxam의 잔류특성을 구명하고 가공계수를 산출한 후 Codex 및 딸기, 토마토 수입국의 농약 잔류허용기준 설정 근거 자료로 활용하기 위하여 이 연구를 수행하였다. 포장시험의 수행은 우리나라 주 산지와 지역을 고려하여 딸기시험포장 지역은 경남 밀양, 경북 구미 및 충북 청주에서 수행하였으며, harvest 3포장, deccline 1포장으로 안전사용기준에 준하여 시험농약을 살포하였다. 토마토시험 포장지역은 전북 익산, 충북 청주에서 수행하였으며, harvest 2포장으로 안전사용기준에 준하여 시험농약을 살포하였다. 경북 김천에서는 포장시험 이 진행 중이며 harvest 및 decline 시험을 안전사용기준에 준하여 현재 진행하고 있다. 또한 시험 농약의 토마토 중 누적 잔류특성을 구명하기 위하여 토마토 가공 제조법으로 퓨레 및 쥬스로 제조 한후 토마토와 가공품 중 잔류농약을 분석할 예정이다. Thiamethoxam의 딸기 및 토마토의 분석 법은 식품공전의 기준 및 시험법을 참고로 하여 분석 회수율 시험을 진행하고 있다.

GC-MS/MS를 이용한 인삼 및 인삼가공품 중 잔류농약 동시다성분 분석법의 개발

박소현 ( So-hyun Park ),정진욱 ( Jin-wook Jung ),전영환 ( Young-hwan Jeon ),정혜현 ( Hye-hyun Jung ),심재룡 ( Jae-ryong Shim ),배병진 ( Byung-jin Bae ),채석 ( Seok Chai ),박종우 ( Jong-woo Park ),김태화 ( Tae-hwa Kim ) 한국환경농학회 2017 한국환경농학회 학술대회집 Vol.2017 No.-

인삼은 우리나라 국민이 가장 많이 이용하는 건강기능식품으로 인삼의 생산 및 유통과정에서 사용되는 각종 농약의 잔류에 의한 오염이 발생됨에 따라 인삼 및 인삼가공품에 대한 잔류농약의 안전관리를 위한 체계적인 잔류량 검사 및 안전성 평가 연구의 수행이 요구되고 있다. 따라서, 본 연 구에서는 현재 국내 인삼 및 인삼가공품에 등록된 83종의 농약에 대한 표준품을 GC-MS/MS를 이용하여 scanning 하였으며 서로 다른 추출법을 통해 간단하고 재현성이 뛰어난 동시분석방법을 개 발하고자 하였다. 인삼 및 인삼가공품에 등록된 농약 중 dithiocabamate계 농약을 제외한 농약 및 대사물을 포함한 105종의 잔류농약을 GC-MS/MS를 이용하여 scanning한 결과, 분석가능한 농약은 54개가 검출 되었다. 또한 다종농약다성분 분석법-제2법(식품공전)과 인삼 다성분 분석법을 이용하 여 추출 및 전처리를 시도하였다. 다종농약다성분 분석법-제2법으로 54개 농약의 회수율을 확인한 결과 회수율은 5.4~109.3%의 범위로 나타났으며 70~120% 범위를 만족하는 농약은 인삼 및 가공 품별로 44~47개로 나타났다. 또한, 인삼 다성분 분석법의 인삼시료 분석법으로 25개 농약의 회수 율을 확인한 결과 회수율은 43.3~113.3%의 범위로 나타났으며 70~120% 범위를 만족하는 농약은 인삼 및 가공품별로 20~22개로 나타났다.