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Field-flow fractionation: A gentle separation and characterization technique in biomedicine
Zhang, Xiaoyue,Li, Yueqiu,Shen, Shigang,Lee, Seungho,Dou, Haiyang Elsevier 2018 Trends in analytical chemistry Vol.108 No.-
<P><B>Abstract</B></P> <P>Field-flow fractionation (FFF), a gentle tool for separation and characterization of macromolecules and particles, has attracted increasing interest in recent years due to its broad dynamic size range and the utilization of an “open channel” void of packing materials or stationary phase. In this review, the theoretical principle of FFF separation (such as retention ratio and external force) is described. The FFF sub-techniques based on the type of the external force employed are presented. Sample physical properties directly measured by FFF sub-techniques are summarized. This paper will critically review the applications of FFF in the field of biomedicine, especially those reported in the last five years.</P>
( Lifang Yue ),( Hexin Lv ),( Jing Zhen ),( Shengping Jiang ),( Shiru Jia ),( Shigang Shen ),( Lu Gao ),( Yujie Dai ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.4
Preservation of fresh algae plays an important role in algae seed subculture and aquaculture. The determination and examination of the changes of cell viability, composition, and bacterial species during storage would help to take suitable preservation methods to prolong the preservation time of fresh algae. Nostoc flagelliforme is a kind of edible cyanobacterium with important herbal and dietary values. This article investigated the changes of bacterial species and biochemical characteristics of fresh N. flagelliforme concentrate during natural storage. It was found that the viability of cells decreased along with the storage time. Fourteen bacteria strains in the algae concentrate were identified by PCR-DGGE and were grouped into four phyla, including Cyanobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. Among them, Enterococcus viikkiensis may be a concern in the preservation. Eleven volatile organic compounds were identified from N. flagelliforme cells, in which geosmin could be treated as an indicator of the freshness of N. flagelliforme. The occurrence of indole compound may be an indicator of the degradation of cells.