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Kang-seuk Choi,Jin-ju Nah,Young-joon Ko,Shien-young Kang,Yi-seol Joo 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2
of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal AntibodyKang-seuk Choi*, Jin-ju Nah, Young-joon Ko, Shien-young Kang1 and Yi-seok JooNational Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea1Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University, 48 Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk 361-763, KoreaReceived April 2, 2003 / Accept July 10, 2003J. Vet. Sci. (2003), 4(2), 167-173JOURNAL OFVeterinaryScience*Corresponding author: Kang-seuk Choi National Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea Tel: +82-31-467-1860, Fax: +82-31-449-5882 E-mail: choiks@nvrqs.go.kr
Studies on the pathogenesis of group A avian rotavirus infection in turkeys
Kang, Shien-young,Nagaraja, Kakambi V.,Newman, John A. The Korean Society of Veterinary Science 1993 大韓獸醫學會誌 Vol.33 No.2
조류 로타바이러스 감염 음성인 1일령, 7일령, 14일령 그리고 21일령된 칠면조에 조류 로타바이러스를 인공감염시킨 후 소장에서의 바이러스의 분포, 배출 그리고 소장의 흡수기능을 조사하였다. 1일령, 7일령 그리고 14일령의 칠면조는 조류 로타바이러스에 대한 혈중모체이행항체의 존재에도 불구하고 로타바이러스 감염에 민감하였다. 인공감염된 칠면조는 경미한 임상증상을 보였으며 십이지장, 공장 그리고 회장의 성숙된 용모상피세포에서 바이러스가 검색되었다. 병리조직학적인 소견으로서는 상피세포에 공포가 형성되었으며 호중구의 침윤이 관찰되었다. 조류 로타바이러스에 인공감염된 14일령 칠면조에서 감염후 1일과 3일에 D-xylose흡수기능은 대조군과 비교하였을 때 유의성이 인정되었다. Commercial turkey poults not previously exposed to avian rotavirus were inoculated orally with the virus alone or in combination with E coki serotype 078 at 1, 7, 14 and 21 days of age. Turkey poults of 1, 7 and 14 days of age were susceptible to infection despite the presence of maternal antibodies against avian rotavirus in their serum. However, turkey poults at 21 days of age were less susceptible compared to those ages 1, 7 and 14 days. The clinical signs in poults of all ages were mild. Viral antigens were demonstrated in the mature villous epithelial cells of the duodenum, jejunum and ilem. Histopathological lesions were characterized by vacuolation of the epithelial cells and heterophil infiltration in infected turkey poults. A significant difference in D-xylose absorption was observed between control and rotavirus infected groups at 1 and 3 days post-infection in 14 days old turkey poults.
Choi, Kang-Seuk,Nah, Jin-Ju,Ko, Young-Joon,Kang, Shien-Young,Yoon, Kyoung-Jin,Jo, Nam-In American Society for Microbiology 2005 Clinical and diagnostic laboratory immunology Vol.12 No.1
<B>ABSTRACT</B><P>Attempts were made to identify and map epitopes on the nucleocapsid (N) protein of peste des petits ruminants virus (PPRV) (Nigeria75/1 strain) using seven monoclonal antibodies (MAbs) and deletion mutants. At least four antigenic domains (A-I, A-II, C-I, and C-II) were identified using the MAbs. Domains A-I (MAb 33-4) and A-II (MAbs 38-4, P-3H12, and P-13A9) were determined to be located on the amino-terminal half (amino acids [aa] 1 to 262), and domains C-I (P-14C6) and C-II (P-9H10 and P-11A6) were within the carboxy-terminal region (aa 448 to 521). Nonreciprocal competition between A-II MAbs and MAbs to C-I and C-II domains was observed, indicating that they may be exposed on the surface of the N protein and spatially overlap each other. Blocking or competitive enzyme-linked immunosorbent assay studies using PPRV serum antibodies revealed that epitopes on the domains A-II and C-II were immunodominant, whereas those on the domains A-I and C-I were not. The competition between MAb and rinderpest virus (RPV) serum antibodies raised against RPV strain LATC was found in two epitopes (P-3H12 and P-13A9) on the domain A-II, indicating that these epitopes may cause cross-reactivity between PPRV and RPV. Identification of immunodominant but PPRV-specific epitopes and domains will provide the foundation in designing an N-protein-based diagnostic immunoassay for PPRV.</P>
Choi, Kang-Seuk,Ko, Young-Joon,Nah, Jin-Ju,Kim, Yong-Joo,Kang, Shien-Young,Yoon, Kyoung-Jin,Joo, Yi-Seok American Society for Microbiology 2007 Clinical and vaccine immunology Vol.14 No.2
<B>ABSTRACT</B><P>A rapid immunoassay for detecting and quantifying West Nile virus (WNV)-neutralizing antibodies in sera was developed as an alternative to the plaque reduction neutralization test (PRNT), the gold standard test for WNV. The assay is a competitive, enzyme-linked immunosorbent assay using neutralizing monoclonal antibody 5E8 (NT-ELISA). A cutoff percent inhibition (PI) value of 35% (mean PI plus 3 standard deviations), with a specificity of 99%, was established based on analysis of 246 serum samples from horses free of WNV. The NT-ELISA detected neutralizing antibodies in all sera collected 7 or 14 days postinoculation from mice (<I>n</I> = 11) infected with lineage I (strain NY385-99) or II (strain B956) WNV. When sera from WNV-vaccinated horses (<I>n</I> = 212) were tested by NT-ELISA and PRNT, the NT-ELISA gave a positive result for 96.1% (173/180) of the PRNT-positive sera and 3.1% (1/32) of the PRNT-negative sera. Discrepancies between the two tests were observed mainly with sera with low PRNT90 titers (expressed as the reciprocal of the highest dilution yielding ≥90% reduction in the number of plaques) for WNV or low PIs by NT-ELISA. The overall agreement (<I>k</I> value) between the two tests was 0.86. A good correlation (<I>r</I><SUP>2</SUP> = 0.77) was also observed between the tests for endpoint titration of sera (<I>n</I> = 116). In conclusion, the newly developed NT-ELISA may be a good alternative serologic assay for detecting WNV that can be used for large-scale testing of WNV-neutralizing antibodies in multiple species.</P>
돼지 group C 로타바이러스 VP6 특이 단클론항체
윤영심 ( Young Sim Yoon ),이승철 ( Seung Chul Lee ),우상규 ( Sang Kyu Woo ),조경오 ( Kyoung Oh Cho ),강신영 ( Shien Young Kang ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.3
Rotaviruses have been known to be a major etiological agent of gastroenteritis in both infants and young animals. Subsequently new rotaviruses, which were morphologically indistinguishable but antigenically and electrophoretically distinct with each other, were reported from several animals throughout world including Korea. These new rotaviruses were named as non-group A or group B or group C rotaviruses and so on. It has been very difficult to isolate and grow the non-group A rotaviruses in vitro, and this has greatly limited the characterizations of non-group A rotaviruses and serological studies. In this study, monoclonal antibodies (MAbs) against porcine non-group A rotavirus were produced and characterized. The VP6 gene of porcine group C rotavirus Korean isolate(#06-52-1) was cloned and expressed. For expression of VP6 gene, baculovirus expression system was applied. The VP6 gene and expressed protein in the recombinant virus were confirmed by polymerase chain reaction (PCR), indirect fluorescence antibody (IFA) test and Western blot, respectively. The expressed VP6 was used for MAbs production. The MAbs produced in this study would be promising as diagnostic reagents for detection of group C rotavirus infection.