Choline dehydrogenase interacts with SQSTM1/p62 to recruit LC3 and stimulate mitophagy.

Park, Sungwoo,Choi, Seon-Guk,Yoo, Seung-Min,Son, Jin H,Jung, Yong-Keun Landes Bioscience 2014 AUTOPHAGY Vol.10 No.11

<P>CHDH (choline dehydrogenase) is an enzyme catalyzing the dehydrogenation of choline to betaine aldehyde in mitochondria. Apart from this well-known activity, we report here a pivotal role of CHDH in mitophagy. Knockdown of CHDH expression impairs CCCP-induced mitophagy and PARK2/parkin-mediated clearance of mitochondria in mammalian cells, including HeLa cells and SN4741 dopaminergic neuronal cells. Conversely, overexpression of CHDH accelerates PARK2-mediated mitophagy. CHDH is found on both the outer and inner membranes of mitochondria in resting cells. Interestingly, upon induction of mitophagy, CHDH accumulates on the outer membrane in a mitochondrial potential-dependent manner. We found that CHDH is not a substrate of PARK2 but interacts with SQSTM1 independently of PARK2 to recruit SQSTM1 into depolarized mitochondria. The FB1 domain of CHDH is exposed to the cytosol and is required for the interaction with SQSTM1, and overexpression of the FB1 domain only in cytosol reduces CCCP-induced mitochondrial degradation via competitive interaction with SQSTM1. In addition, CHDH, but not the CHDH FB1 deletion mutant, forms a ternary protein complex with SQSTM1 and MAP1LC3 (LC3), leading to loading of LC3 onto the damaged mitochondria via SQSTM1. Further, CHDH is crucial to the mitophagy induced by MPP+ in SN4741 cells. Overall, our results suggest that CHDH is required for PARK2-mediated mitophagy for the recruitment of SQSTM1 and LC3 onto the mitochondria for cargo recognition.</P>

파트너십에 의한 지하철 입구 공간의 쌈지공원 설계 및 시공

김승환,강현우,손용욱,박승범,남정칠,이기철,차민준 東亞大學校 建設技術硏究所 2009 硏究論文集 Vol.32 No.2

A pocket park is valued in securing green space in downtown and improving urban view in the aspect of natural and environmental values, and it signifies a lot as the center of local residents’ community and harmony as well. Since subway exits and surroundings that have high portions in taking up street space due to lack of green space in downtown aren't made full use of, valuable pocket park place. Dangni Pocket Park of subway exits is a park completed with cooperative works and partnership by students, specialists, administrative organizations, businesses and civilian organizations, etc. without support of special budget. The study site was finally chosen to the space between exit No.1 of Dangni subway station, after analyzing the surrounding circumstances, availability and accessibility of the space among the several subway station exits of Saha, Dangni, and Hadan Station in Busan Metropolitan city. For the designing process, The Citizens organization for Million Amenity Park performed the planning and project proceeding, and 19 design drafts were proposed during the class of landscape architecture in Dong-a University. Design review panel members discussed to select the best draft in practical and authentical, and the related experts reviewed and revised to present the final design plan. The construction was conducted was performed under the cooperation between the civil, authorities, and industrial organizations, among which Saha District office managed for the waste treatment and maintenance matters. The graduates of landscape architecture department of Dong-a University and related industrial enterprises supplied landscape materials and related labors, were supported by 20 members of GPM, one of clubs of Dong-a University volunteered durig the most of construction progress, completing the Pocket Park of subway exits through partnership.

대중교통 환승주차장의 CPTED 체크리스트에 의한 환경디자인 연구

김대용(Kim, Dae Yong),김태용(Kim, Tae Yong),정승호(Chung, Seung Ho),박현호(Park, Hyeon Ho) 한국전시산업융합연구원 2012 한국과학예술융합학회 Vol.10 No.-

본 연구는 늘어나는 대중교통인접 환승주차장의 범죄위험성으로부터 이용자들의 안전을 위하여 ‘환경설계를 통한 범죄예방(Crime Prevention Through Environment Design: CPTED, 이하: CPTED)"개념을 적용하여, 환경디자인 개선사항을 도출하는 연구이다. 연구의 방법과 범위로는 CPTED이론, 문헌분석, 관련법 및 법규, 관련 전문가그룹을 통하여 1차 정량·정성체크리스트를 설계하여 현장답사 하였으며, 이를 보안하는 2차 정성체크리스트를 설계, 현장답사 하였으며, 현장답사는 대중교통환승 주차장의 유형과 환승형태, 주차장규모, 운영주체등을 고려하여 수도권의 5개 환승주차장을 선정하였다. 설계된 체크리스트를 바탕으로 현장답사 결과 범죄위험성으로부터 환경디자인 개선사항은 5곳 중 1곳을 제외하고 4곳 모두 개선이 필요한 것으로 분석되었다. 분석은 주차지역, 유지관리, 전자보안, 안내표지, 조경, 조명으로 구분하였으며 조사대상지 대부분 범죄위험성으로부터 전자보안에만 의지하는 행태를 가졌으며, 그 또한 요금 징수 등을 주 목적으로 하고 있다. This study applies the concept of Crime Prevention Through Environmental Design (CPTED) and suggests ways to make improvements in environmental design to provide secure facilities for those who use park-and-ride structures which are increasing on a regular basis. First, the authors made a checklist for field examination after consulting related literature and related laws and holding discussion sessions with the experts of the field. After the field investigation with the quantitative and qualitative checklist, another qualitative checklist was developed to supplement the first one. With the second checklist, the authors visited five different park-and-ride structures around the city of Seoul that were diverse in layout, scale, and management system. The result showed that four out of the five sites assessed needed improvements. The analysis was divided into the following areas: location of the structure, maintenance, electronic security, signs, landscaping, and lighting. A popular method of crime prevention at the parking structures was electronic security which was in fact operated mainly to encourage the drivers to pay their parking fees.

당뇨병환자에서 뇨증 Fibronectin농도의 증가에 관한 연구

한승범,조준승,손건영,서성문,박근용,조성래,박규영,박정모,이인규,여준기 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.4

Plasma fibronectin is an α₂-glyoprotein, which is produced by vascular endothelial cells. Raised level of plasma fibronectin has been observed in diabetic patients particularly in the presence of microvascular complications. However, no available data exist about urine level of this glycoprotein in diabetic patients. We measured urine fibronectin level by ELISA methods in 54 diabetic patients who have microvascular comlications or not. The following results were obtained. 1) Urine fibronectin level(㎍/g creatinine) in diabetic group(1740.0±678.0) is increased compaired with those in normal control group(471.0±59.0). but this results were not significantly different among two groups(P>0.05). 2) Urine fibronectin level(㎍/g creatinine) was significantly increased in patients with nephropathy (6188.0±3144.0) compared with those in normal control group(471.0±59.0) and patients without nephropathy(645.0±251.0)(P<0.001). 3) There were a significant correlation between BUN, creatinine, creatinine clearance, 24hr urine total protein and urine fibronectin level in diabetic patients. Our data suggest that urine fibronectin excretion level might be used as a sensitive guide for diabectic nephropathy.

우심방을 침범한 거대 비호지킨씨 림프종 1예

강상범,진승원,이은경,박용현,최용호,김용주,박준철,김재형,홍순조,최규보,권종범,원용순,박건,이은희 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

Reference Map of Soluble Protenis from Salmonella enterica Serovar Enteritidis by Two-Dimensional Electrophoresis

김곤섭,Eung-goo Lee,Yong-hwan Kim,정태성,Yong-seung Shin,Gee-wook Shin,Hui-guen Cha,Mi-rim Park 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Map of Soluble Proteins from Salmonella enterica Serovar Enteritidis byTwo-Dimensional ElectrophoresisMi-rim Park, Eung-goo Lee, Yong-hwan Kim, Tae-sung Jung, Yong-seung Shin, Gee-wook Shin, Hui-guen Cha and Gon-sup Kim*Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, KoreaReceived May 17, 2003 / Accepted July 23, 2003J. Vet. Sci. (2003), 4(2), 143-149JOURNAL OFVeterinaryScience*Corresponding author: Gon-sup Kim Laboratory of Veterinary Biochemistry, Collage of Veterinary Medicine, Gyeongsang National University, 900 Gazwa, Jinju 660-701 Korea Tel: +82-55-751-5823, Fax: +82-55-751-5803 E-mail: gonskim@nongae.gsnu.ac.kr

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

가스크로마토그래피/질량분석법에 의한 최적화 유도화 과정을 통한 Atrazine의 새로운 분석방법 개발

박승건,김승진,유영재,이용일 昌原大學校 基礎科學硏究所 2003 基礎科學硏究所論文集 Vol.15 No.-

Pesticides, with their high degrees of toxicity constitute a very impOrtant group target compound in environment samples. The use of many pesticides has been restricted or even banned while several years bemuse of their long residue times, persistence and bio-accumulation. Atrazine was derivatized using a single-step procedure by N,O-bis(trimathylsilyl)-acetamide acetonitrile in various solvent systems such as methanol, ethyl acetate, pyridine, and tetrahy&ofuran(Tl). The variables selected for study were the amount of reagents, the temperature and reaction times. The elelctron impact ionization mass spectra of the resulting derivatives are acquired and properly interpreted. Under the optinaized gas chromatographic conditions employed, an enhanced (3 times) peak height and an improved peak shape without any tailing. The purpose of this study was the development and application of GC-MS method for efficient analysis of atrazine.

상악 중절치부위의 과잉치에 대한 증례보고

박희승,정향숙,김용기,정희민 大韓小兒齒科學會 1990 大韓小兒齒科學會誌 Vol.17 No.2

The term “Mesiodens” refers to a supernumerary tooth that is present in the midline of the anterior maxilla. Supernumerary tooth results from aberrations in the initiation or proliferation period of dental developmental stage. The crown often has a conical shape, and its familial incidence has been reported in several instances. The author has observed 71 cases of supernumerary tooth occured at premaxilla between central incisors. Among the total tooth number of 86,70 were impacted, and 16 were erupted. In most cases(96.5%), supernumerary tooth located on the palatal side of adjacent teeth. Surgical and/or orthodontic treatment were performed in 53 cases, 21 cases, were left untreated for delayed surgical intervention.

한계상태설계법에 의한 부분 프리스트레스트 콘크리트 부재의 설계프로그래밍

박홍용,이승열 명지대학교 대학원 1997 대학원논문집 Vol.1 No.-

Partially prestressed concrete members are concrete members reinforced with a combination of prestressed and non-prestressed reinforcement, and also can offer advantages of reinforced concrete and prestressed concrete. The purpose of this study is to develope a design program using Visual Basic and Fortran language according to the limit state design method which controls crack check fatigue effectively, for partially prestressed concrete members. The limit state design method has not been used in domestic, so in this study refers to Japan highway bridge design specification and concrete design specification. In this study, partially prestressed concrete 3-span TT-section girder bridge model prestressed concrete TT-deck bridge model were checked by developed program.