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벼 葯培養에 關한 硏究 Ⅴ. 母植物의 生理的 stress에 의한 統一型 品種의 葯培養 效率增大
Seung Yeob Lee(李承燁),Young Tae Le(李暎泰),Hyun Soon Kim(金賢順) 한국육종학회 1988 한국육종학회지 Vol.20 No.3
For the increase of anther culture efficiency, the environmental conditions for donor plants on the androgenic response and on the pollen dimorphism in vivo was investigated in three tongil type cultivars Seogwangbyeo, Samgangbyeo, and Pungsabyeo, which had low sporophytic potential. Donor plants were grown under two day-lengths, 8 and 16 hours and three nitrogen levels, 0, 15, and 30kg/10a, respectively. The cold-pretreatment for anthers was only treated to three nitrogen levels for 10 days at 10℃. The number of N-and S- pollen grains in vitro was compared to japonica type cultivars showing high sporophytic potential after 30 days in culture. Effect of day length on callus formation and on plant regeneration was different among cultivars. But S-pollen grains increased by the day length treatments in Seogwangbyeo and Pungsanbyeo. The rates of S-pollen grains and callus formation were significantly increased in 0 and 30kg/10a nitrogen levels. Callus formation and plant regeneration were remarkably increased by the interaction between nitrogen stress and cold pretreatment. Samgangbyeo, grown under nitrogen-free condition, showed the highest reaction as 37.4% and 12.0%, respectively. In vitro the development of N pollen grains form inoculated anther of tongil type cultivars was more noticeable than in japonica type cultivars, Tongil type cultivars seemed to be low callus formation because they have high gametophytic potential in vitro.
Panaxynol, a natural Hsp90 inhibitor, effectively targets both lung cancer stem and non-stem cells
Le, Huong Thuy,Nguyen, Huy Truong,Min, Hye-Young,Hyun, Seung Yeob,Kwon, Soonbum,Lee, Yeongcheol,Le, Thi Hong Van,Lee, Jeeyeon,Park, Jeong Hill,Lee, Ho-Young Elsevier 2018 Cancer letters Vol.412 No.-
<P><B>Abstract</B></P> <P>Cancer stem-like cells (CSCs) contribute to tumor recurrence and chemoresistance. Hence, strategies targeting CSCs are crucial for effective anticancer therapies. Here, we demonstrate the capacities of the non-saponin fraction of <I>Panax ginseng</I> and its active principle panaxynol to inhibit Hsp90 function and viability of both non-CSC and CSC populations of NSCLC <I>in vitro</I> and <I>in vivo.</I> Panaxynol inhibited the sphere forming ability of NSCLC CSCs at nanomolar concentrations, and micromolar concentrations of panaxynol suppressed the viability of NSCLC cells (non-CSCs) and their sublines carrying acquired chemoresistance with minimal effect on normal cells derived from various organs. Orally administered panaxynol significantly reduced lung tumorigenesis in <I>Kras</I> <SUP> <I>G12D/+</I> </SUP> transgenic mice and mice carrying NSCLC xenografts without detectable toxicity. Mechanistically, panaxynol disrupted Hsp90 function by binding to the N-terminal and C-terminal ATP-binding pockets of Hsp90 without increasing Hsp70 expression. These data suggest the potential of panaxynol as a natural Hsp90 inhibitor targeting both the N-terminal and C-terminal of Hsp90 with limited toxicities.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Panaxynol inhibited the sphere-forming ability of NSCLC CSCs by inducing apoptosis. </LI> <LI> Panaxynol suppressed the viability of NSCLC cells with no effects on normal cells. </LI> <LI> Panaxynol significantly reduced lung tumorigenesis without detectable toxicity. </LI> <LI> Panaxynol interacted with the N- and C-terminal ATP binding pockets of Hsp90. </LI> <LI> Panaxynol disrupted Hsp90 function without inducing Hsp70 expression. </LI> </UL> </P>
Poster Presentation : Malignant Mixed Tumor in the Salivary Gland of a Dog
( Il Hwa Hong ),( Jin Kyu Park ),( Dong Wei Yuan ),( Ok Kyung Hwang ),( Kyung Sook Hong ),( Jung Youn Han ),( Ae Ri Ji ),( Mi Ran Ki ),( Sun Hee Do ),( Tae Hwan Kim ),( Chang Woo Min ),( Seung Yeob Le 한국수의병리학회 2008 학술대회 Vol.12 No.2