Production and Characterization of a Thermo-alkalotolerant Cyclodextrin Glucanotransferase from Thermo-alkalophilic Bacillus cereus B-13

Seo, Seung-Bo,Kim, Jae-Ho,Lee, Dae-Hyoung,Lee, Jong-Soo 배재대학교 자연과학연구소 2005 自然科學論文集 Vol.16 No.1

내열성의 cyclomaltodextrin glucanotransferase (CGTase)를 이용하여 열에 안정한 싸이클린 텍스트린 (CD)을 생산하기 위하여 매우 높은 CGTase 활성을 보이는 고열성이며 호알카리성인 B-13 세균을 분리하여 형태적, 생리학적 특성과 16S 리보솜 RNA 서열분석 등을 통하여 Bacillus cereus B-13으로 동정하였다. Bacillus cereus B-13을 2% 가용성 전분, 1% 효모 추출물, 1% Na_(2)CO_(3) 등을 함유하는 SYC 배지 (pH 8.5)에 접종하여 50℃에서 24시간 배양하였을 때 최고의 CGTase 활성(130 U/ml)을 보였다. 또한 부분 정제된 CGTase의 작용 최적 온도와 pH는 각각 65℃, pH 8.5-9.0 이었고 80℃이하와 pH 5.0-10.0에서 안정하였다. 1% 가용성 전분을 부분 정제한 CGTase와 작용 시켰을 때 49%의 CD 수율을 보였다. To produce a thermostable cyclodextrin by using thermotolerant cyclomaltodextrin glucanotransferase(CGTase), a thermophilic and alkalophilic bacterium isolate, designated B-13 showing the highest CGTase activity was isolated from natural sources and identified as Bacillus cereus B-13 based on the morphological and physiological characteristics, and 16S rRNA sequence. The maximal CGTase activity (130 U/ml) was obtained when Bacillus cereus B-13 was cultured in SYC medium containing 2.0% soluble starch, 1.0% yeast extracts, 1% corn steep liquor and 1% Na_(2)CO_(3) (pH 8.5) at 50℃ for 24 h and about 80% of maximal activity was also showed in the culture broth of 60℃ for 18 h . Optimum reaction temperature and pH of the partial purified CGTase for soluble starch were 65℃ and pH 8.5-9.0, respectively. The partial purified CGTase were also stable below 80℃ and pH 5.0-10.0. When 1% soluble starch was digested with the partial purified CGTase, the yield of cyclodextrin was 49%.

인조 합성 테니스 스포츠 바닥재의 운동생리학적 분석

서혜림,박승범,서국웅,이재규,서혜진 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

The purpose of this study was to investigate the changes of heart rate(HR), Ventileation, Carbon elimination, Oxygen take, Oxygen uptake per weight, Respiratory Exchange ratio and Breath frequency. To do this, we selected three Synthetic tennis playing surface(A:Synpave Rebound Ace Sample, B:Synpave Rebound Classic Sample, C:Synpave Spring Sample) by using of Austrilian Tennis Open. The result of this study is as follows 1. In the changes of heart rate(HR, beats/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample C, sample C was shown a little bit higher than sample B, 2. In the changes of Ventileation(VE, 1/min), it is not significantly in a stastics. Tennis playing surface sample B was shown a little bit higher than sample A·C, sample C was shown a little bit higher than sample A, 3. In the changes of Carbon elimination (Vco2, ml/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample B was shown a little bit higher than sample C, 4. In the changes of Oxygen take (Vo2, ml/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample B was shown a little bit higher than sample C, 5. In the changes of Oxygen uptake per weight(Vo2/wt, ml/kg/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample B was shown a little bit higher than sample C, 6. In the changes of Respiratory Exchange ratio(RER, Vco2/Vo2), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample C was shown a little bit higher than sample B, 7. In the changes of Breath frequency (BF, fre/min), it is not significantly in a stastics. Tennis playing surface sample B was shown a little bit higher than sample A·C.

초등학교 제 7차 수학과 교육과정 운영 실태 분석

서승희,김민경 이화여자대학교 사범대학 교육과학연구소 2002 교육과학연구 Vol.33 No.2

이 연구는 개정된 제 7차 수학과 교육과정을 현장에서 실천하고 있는 초등 교사들을 대상으로 교육과정 운영 실태를 분석하고 현장 적용에 따른 문제점을 조사하여 개선안을 모색하고자 하였다. 이를 위해 서울시내 초등교사 1,2,3,4학년 담임교사를 대상으로 설문조사를 실시하였다. 조사 결과, 교육과정의 원활한 운영을 위한 사전연수미비, 교사들의 단위학교 수학과 교육과정개발에 대한 관심과 참여가 저조하였음이 나타났다. 또한 단계형 수준별 교육과정의 도입 취지에 대해서는 긍정적이었으나 과밀학급, 물·인적 자원지원체제 미비 등 교육환경적 요인과 보충과정 운영 및 재이수 문제 등 교육과정 자체요인으로 인해 단계형 수준별 교육과정 운영에 어려움을 겪고 있는 것으로 나타났다. 이에 국가수준의 교육과정 의도를 성공적으로 현장에 정착시키기 위해 국가 차원의 체계적이고 집중적인 교원연수 및 물·인적 자원의 지속적인 지원이 필요하며, 교사들의 합의와 숙의 과정을 통해 각 단위학교 실정에 적합한 수학과 교육과정을 개발하고, 교육운영방식을 자율적으로 선택하고 개발할 수 있도록 지원해 주어야 할 필요성이 제기되었다. The purpose of the study is investigate the way how the 7th revised elementary mathematics curriculum is recognized by teachers at school and how it is being implemented by them. This study is based on the results of the survey over teachers who teach the first, second, third and fourth graders and observations of sample classes. The survey was focused on finding out how teachers recognized, operated and adjusted the 7th mathematics curriculum. The results of the survey were as follows. The 7th mathematics curriculum was generally recognized by most teachers through inside and outside school training, not very well nor not very bad. The surveyed teachers, however, were not well aware of the program and operation of curriculum since not many of them participated in the procedures of adjusting the curriculum to each school at the beginning of the semester. It was founded that most teacher participants in survey agreed to the integration of the differentiated curriculum into classroom. However, they regarded overly populated classes, lack of supporting systems and materials, and level education as main obstacles in adjusting the 7th mathematics curriculum. Based on the results of the survey, this study suggests the followings in order to make the 7th revised mathematics curriculum be better implemented at school. Recognition and understanding of the new curriculum by teachers should be continuously enhanced through various formal and informal trainings given by national, municipal or provincial education authorities. And educational authorities should keep supporting systematic materials and information. More deliberation should be made on the differentiated curriculum which many teachers agree to find difficulty adopting at school. Each school should have more discussion on this matter and try to find way to adjust to individual school under its unique circumstances.

에어로빅 댄스 Kick 동작의 운동역학적 분석

서국웅,이훈식,윤양진,정미라,박승범 釜山大學校 附設 體育科學硏究所 1998 體育科學硏究所 論文集 Vol.14 No.-

The purpose of this study is to investigate the front and side Maximum Vertical Ground Reaction Force's of Knee-up, long-kick and high-kick, the effect of them speed rate on Maximum Vertical Ground Reaction Force, and the damage factor caused by them, and to provide useful data for ordinary people or athlete aerobic programs. The result of this study is as follows ; 1. Maximum Vertical Ground Reaction Force is not significant between the front and the side. 2. Maximum Vertical Ground Reaction Force is significant between differences of speed rate. 3. Maximum Vertical Ground Reaction Force, speed and direction being equal, is significant according to the motion types: knee-up, long-kick and high-kick. 4. The possibility of causing damage is the highest when Maximum Vertical Ground Reaction Force is more than 20BW/sec. 5. High-kick, which needs quick and heavy motion, is considered not be fit for beginners. 6. Load-rate must be lowered by choosing adequate speed rate and motion type, and even in the career of skilled athletes, enough exercise and muscle strength training of legs are necessary in doing aerobic.

초등학교 과학과 에너지영역의 STS 내용 변천

서승조,조태호,백남권,김성규,박강은,정인재 晋州敎育大學校 科學敎育硏究所 2001 科學敎育硏究 Vol.27 No.-

This research compared and analyzed the content ratio of the STS (Science-Technology-Society) Education and subjects and types of learning activities to determine the characteristics of STS education in science classes of elementary schools. In the 5th curriculum, the STS-related content on energy section took up 6.6% , in the 6th curriculum, compared with that of the 5th, the STS content ratio on the energy section increased 7.9% and the STS content on the energy section in the 7th curriculum showed an increased 20.8% proportion. Comparing the main subjects of the STS in the energy section for the science class according to curriculum in the 5th, they obviously have influenced technological development, energy, the development of space studies, and sociology of science, in that order. In the 6th curriculum. compared with that of the 5th, the energy section decreased and the sociology of science increased much. There were no significant changes in other subjects. In the 7th, the STS elements were distributed evenly but the extent of the STS main subjects reduced compared to that of the 6th. The STS education activities represented in science textbooks of elementary schools were mainly executed through discussion, research activities, and actual activities. Other activities such as role-playing, simulation tests, problem solving and decision making, data analysis, research design, case studies, etc. were hardly made.

초등학교 교사들의 과학과 실험연수에 대한 의식 조사

서승조,조태호,백남권,김성규,박강은,정순호 晋州敎育大學校 科學敎育硏究所 2001 科學敎育硏究 Vol.27 No.-

This study investigated the perceptions of elementary school teachers' on the science experimental training course using closed-form questionnare. 437 elementary school teacher, 21 school, 14 cities and counties in Gyung Nam province were selected as the objects for researching these matters. The results were as follows; First, without system and holding type teachers choose on the training not the same time but their ability freely. Second, to extend the trust the composition of place and instructor must be based on professor and research in the national university of education. Third, the composition of the text must be made of scientific growth based on important experimental activities teaching strategy, the using of materials. It must be increase multimedia training course on the science education. Fourth, the method of training course it must be happen on the experiment and discussion and it is necessary to grow up training level. Fifth, the type of training it must not give the score. Therefore it will be free and comfortable without difficulties. In the results, the improvement of science experimental training course is compatible on the purpose and it will help on the science teaching-learning.

쇼트피닝 가공에 의한 압축잔류응력의 현상해석

서성원,이승호 서울産業大學校 2000 논문집 Vol.51 No.2

Shot peening was applied on both smooth specimen and notch specimen on order to investigate the stress distribution and the improvement in fatigue strength. The results showed that the fatigue strength of shot peened specimens increased compared to that of unopened ones. In this paper, Finite elements method showed that shot peening is effective in decreasing the bending stress by external force. The effectiveness of shot peening in reducing the compressive residual stress was anticipated by the superposition of the concentrated stress and the compressive residual stress.

전문 런닝화 개발을 위한 런닝화 화형 분석 : 2000년 런닝화를 중심으로

서국웅,윤양진,박승범,김용재 부산대학교 체육과학연구소 2000 體育科學硏究所 論文集 Vol.16 No.-

This study was designed to investigate the problem of the existing Running Standard Last for the Male adult and to suggest some useful information for the better design development and construction of running shoes last. The subjects for this study are based on 4 male adults who had the running pattern of rearfoot strike with normal feet. 51 separate measurements of four lasts of running shoe with N, R, AD, AS brand were tested using a 3-mentional measurement. Four running shoes and a barefoot were measured using a pressure distribution measurement. The findings of the study were as follows 1) The N running shoes last was larger than those for R, AD and AS the standard in the 8 items. The AS running shoes last was larger than those of N, R, AD last in the 8 items. The R's last was the biggest in short heel girth and long heel girth. The AD's last was the biggest in seat length and firth toe height 2) In the regional peak pressure of the running sheos, the barefoot and brand running shoes showed the biggest pressure distribution in the hallux, There were the slightest pressure in the medial midfoot and lateral midfoot. 3) The instance of regional peak pressure, the barefoot and the running shoes showed earlier peak pressure for the lateral heel. It was barely possible to measure pressure distribution in medial midfoot. 4) In the light of time percentage of peak pressure distribution measurement in each mask, N running shoes showed the longest time in medial heel, lateral heel, medial midfoot, lateral metatarsal and lateal toes, AD running shoes showed the longest in first metatarsal, second metatarsal and hallux, R running, the longest in second toes. 5) In the maximum force for pressure distribution, barefeet showed the biggest pressure distribution in second metatarsal and R, AD running shoes the biggest in first metatarsal, respectively. 6) In the time of peak pressure distribution measurement, barefeet quickly showed peak pressure distribution in the lateral midfoot. Simultaneously, N. R, AD and AS's running shoes showed peak pressure distribution with barefeet. 7) In the characteristic of the new last's model, bottom length resulted in 276.00mm, stick length 283.29mm, 70% ball mirth 255.12mm, bottom width 91.21mm, toe thickness 30.00mm.

협동학습이 자연과 학업성취 및 과학태도에 미치는 효과

서승조,백남권,조태호,김용진 晋州敎育大學校 科學敎育硏究所 1999 科學敎育硏究 Vol.25 No.-

The purpose of this study is to manifest the effect of STAD cooperative learning which has not yet been explained in cooperative learning of an elementary school, on the academic achievement in science and science related attitudes. Therefore, in order to achieve that purpose, I established the following issues of study. First: What effect does STAD cooperative learning have on the elementary school students' academic achievement in science? Second: What effect does STAD cooperative learning have on the elementary school students' science related attitudes? The results of the study are as follows. First, while it was proven that the effect of STAD cooperative learning on academic achievement in science had no significant difference in pre·post achievement levels for both the experimental group and the control group, the experimental group showed larger changes in the mean value than control group; it can be inferred that classes to which cooperative learning is applied is more effective than traditional science classes. Second, in regard with the effect of STAD cooperative learnign on science related attitudes, while there was a significant difference between the experimental group and control group in pre examination, there was no significant difference, which means STAD cooperative learning does have effect on science related attitudes. In conclusion, the results of this study manifest that STAD cooperative learning is effective for science related attitudes in elementary school science classes, and assume that it can be effective for academic achievement.

두호리 공룡 발자국화석에서 본 공룡의 행동

서승조 진주교육대학교 과학교육연구소 2000 科學敎育硏究 Vol.26 No.-

Three well preserved trackways and one other trackway are found at Dooho-ri, in Goseong County. At the site A, a quadrupedal Sauropod's trackway shows that a trackway, about 20ms long changed its direction from 170˚ to 220˚ in azimuth. It seems that the trackmaker walked along the curved lake-shore line at that time. And at the site B, three bipedal tracks are observed. Trackmaker Dino-1 and -2 walked toward 250˚ in azimuth(approximately WSW) and Dino-3 crossed their trackway from Southwest to Northeast. The study of the variation of Dino-1's pace and direction leads to the conclusion that Dino-3 may have interfered with Dino-1's itinerary.