다카야스 동맥염과 동반된 초점성 분절성 사구체경화증 1예

강신명,송영수,김주성,윤수영,이루다,최규헌,조승연,이호영,한대석,정현주' 대한신장학회 2000 대한신장학회잡지 Vol.19 No.5

위선암에서 p53, c-erbB-2, 그리고 DNA ploidy의 상관관계에 관한 연구

강상균,양승하,김대중,김의한,곽현수 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

Gastric carcinoma is the most common malignant tumor in korea and many oncogenes have found in gastric carcinoma. The relation between the expression of p53 and c-erbB-2 and the prognosis of patient in gastric adenocarcinoma have studied recently, but it is controversy. To evaluate correlation between the pathologic findings including prognostic factors and the expression of p53 and c-erbB-2, and DNA ploidy, we have performed immunohistochemical staining using 83 cases adenocarcinoma and flow cytometry with 56 cases. The results were as follows; 1) The expression rate of p53 was 45.8% in gastric adenocarcinoma. Differentiated, intestinal, advanced, and lymph node metastatic cases showed higher expression rates than poorly differentiated, diffuse, and non-metastatic cases, without statistically significance. 2) The expression rate of c-erbB-2 was 62.6% in gastric adenocainoma. c-erbB-2 was related to differentiated and intestinal types. Advanced and metastatic cases showed higher expression rates of c-erbB-2 than early and non-metastatic cases, but there was not statistic significance 3) Gastric adenocarcinoma showed correlation of DNA diploidy with poorly differentiated type and aneuploidy with differentiated. 4) DNA diploidy and aneuploidy showed correlation with c-erbB-2 negative and postive cases respectively, but correlation between DNA ploidy with p53 expression was not proved. Above results showed that p53 and c-erbB-2 may be related poor prognosis but there was no statistic significance. DNA aneuploidy and diploidy showed correlation with c-erbB-2 expressed and non-expressed cases, respectively, but not with p53 expression.

Paraquat lung에서 Fibronectin 분포에 관한 연구

강상균,양승하,김대중,김의한,김성용 순천향의학연구소 1996 Journal of Soonchunhyang Medical Science Vol.2 No.1

To investigate the process of the Paraquat induced pulmonary fibrosis, 10 mg/Kg and 20mg/kg of paraquat was administered intraperitoneally each of 10 rats respectively. Three, five, seven, ten and fifteen days after this injection, the rats were sacrificed. Immunohistochemical studies for fibronectin and histologic studies were carried out. Results were as follows. 1. Toxic effect of the paraquat seems to be depend on the dose of administered paraquat since the groups of the rate treated with 10 mg/kg revaled no significant tissue damage after 7 days. 2. The pulmonary changes seen in 20 mg/Kg treated group could be classified into two stages: the early changes such as hemorrhage, edema and hyaline membrane formation which are similar to the findings observed in adult respiratory distress syndrome and the late change characterized by fibrosis which observed in mild degree even after 2 weeks, so the fibrosis seen to need more time for full blown. 3. The positivity of fibronectin were observed in macrophage and exudate in the early stage of tissue destruction that the fibronectin productin is preceded fibrosis and plays an important role for the collection of inflammatory cells such as macrophaques and fiboblasts. The more tissue desturction is severe, the more strong positivity of fibronectin was observed in the early stage of tissue destruciton.

Paraquat lung에서 TNF alpha 분포에 관한 연구

강상균,양승하,김대중,김의한,정용민 순천향의학연구소 1996 Journal of Soonchunhyang Medical Science Vol.2 No.1

To investigate the process of the Paraquat induced lung damage, the white rats were administered 20 mg/Kg of Paraquat intraperitoneally. Three, seven, ten, 24 hours, three, five, seven, ten and fifteen days after this injection, the rats were sacrificed. The removed lung were evaluated by tumor necrosis factor and histologic study, so following results were obtained. 1. The significant inflammatory changes appeared after 3 days and most severity was seen in 7 days. The fibrosis appeared after 7 days and the degree was mild through observation period which indicate that the developement of fibrosis need more time. 2. TNF-∝ positivity precedes morphologic changes of the lung induced by Paraquat administratin, then positiviey of TNF-∝ decreases if the tissue destrucion is severe. 3. Early histologic change such as edema and ARDS like finding may be due to vascular leakage, caused by retraction of endothelium influenced by TNF-∝.

위선암에서 E-cadherin의 표출양상에 관한 연구

강동하,김대중,김창진,김의한,양승하 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

Gastric adenocarcinoma is the most common malignant tumor in Korea. Many oncogens have been found in malignant tumor cells. E-cadherin located in cell membrane is important for progression and metastasis of tumor. To evaluate the expression of E-cadherin according to histopathologic features and the relation between E-cadherin and prognosis on gastric adenocarcinoma, we have performed immune staining, using mouse HECD-1 for 97 cases. The results were as follows; 1) Gastric adenocacinoma showed the loss or marked reduction of the expression of E-cadherin in 30.9% of 97 cases, and early carcinoma 34.2% and advanced 28.8%. 2) According to differentiation of gastric adenocarcinoma, cases showing the loss or marked reduction of the expression of E-cadherin was well differentiated form 16.1%, moderately 35.7%, poorly 31.9% and mucinous carcinoma 100%. 3) According to the degree of invasion in early carcinoma, cases showing the loss or marked reduction of the expression of E-cadherin was carcinoma within mucosa 41.2% and carcinoma invaded to submucosa 28.6%, but there is no significant difference according to types. 4) According to absence or presence of lymph node metastasis in advanced carcinoma, cases showing the loss or marked reduction of the expression of E-cadherin was 17.6% in non-metastatic cases and 33.3% in metastatic cases, but there is no significant difference according to degree of invasion. Above results indicated that the loss or marked reduction of the expression of E-cadherin in gastric adenocarcinoma may be related to prognosis of the patients.

Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

대장선암에서 p53 과 P-glycoprotein의 분포에 관한 연구

강상균,김의한,장인성,김대중,양승하 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Adenocarcinoma of colon and rectum express many oncogenes. The p53 acts as tumor suppressor in normal tissue but mutant forms are known as doing important role in carcinogenesis of many malignant tumors, such as colorectal cancer, gastric cancer, and breast cancer. P-glycoprotein shows distribution in many normal tissues and malignant tumor. This protein occurs multidrug resistance to therapeutic anticancer drug, resulting in poor prognosis of patients. The expression of p53 and P-glycoprotein has been studied by many authors according to clinicopthologic findings of colorectal adenocarcinoma, but these results are controversial yet. To observe the relationship between both antigen and pathologic findings, including prognostic factos, of colorectal adenocarcinoma, we analysed 13 cases adenoma and 62 cases adenocarcinoma then processed for immunohistochemical stain by SLAB method using mouse monoclonal anti-p53 and JSB-1. The results were as followings; 1) The expression rate of p53 in adenoma and adenocarcinoma are 38.5% and 71.0%, respectively, and P-glycoprotein are 46.6% and 58.1%, respectively. 2) The p53 revealed higher expression rate in case of well differnetiated form, invasion to serosa and surrounding adipose tissue, lymphatic metastasis compared to that of moderately and poorly differentiated form, invasion to muscle, no lymphatic metastasis, but no difference accoding to site. 3) P-glycoprotein revealed higher positive rate in case of right side, invasion to surrounding adipose tissue, lymphatic metastasis compared to that of left side, invasion to muscle and serosa, no lymphatic metastasis, but no difference according to differentiation. 4) Both of p53 and P-glycoprotein revealed higher ex pression in case of right side, well and moderately differentiated form, invation to serosa and surrounding adipose tissue, lymphatic metastasis compared to that of left side, poorly differentiated form, invasion to muscle, no lymphatic metastasis. Above results revealed that the expression of p53 begins in adenoma and shows close relation with development of colorectal carcinoma. p53 and P-glycoprotein may reveal the relation with poor prognosis. Adenocarcinoma showing both positive reaction of p53 and P-glycoprotein is also similar prognostic relation.

수치사진측량에 의한 고 건축물의 3 차원 정보 획득

姜準默,韓承熙,嚴大鎔 충남대학교 지역개발연구소 2000 地域開發論叢 Vol.12 No.-

This study aim at establishing the 3D digital data of cultural architecture by applying the digital photogrammetric technique. I would applicated as the basic data for preservation and restoration of cultural assets by suggesting the direction of establishing 3D digital data and the more accurate measurement methods than before lagged ones. And I could establish the useful data for visual analysis by connecting the ortho image which was made by digital image for the object and the vector data which was acquired by plotting. The establishment of precise 3D information by photogrammetry was possible, so the application of photogrammetry for measuring various cultural assets which require precise measurement is expected actively in near future.

복막유착의 원인 규명과 예방을 위한 실험적 연구

강대영,송규상,노승무,조준식,이태용 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

Postoperative peritoneal adhesion is the most common cause of intestinal obstruction, and intestinal obstruction is one of the most difficult problems in abdominal surgery. In this experimental study for developing peritoneal adhesion model and its prevention, we divided rats into 5 groups; the talc powder treated group, the cotton-fluff treated group, the talc+fluff treated group, the ischemia group and the control group. There were 16 rats in each group. We made incisions on the midabdomen of the rats and administered talc powder, cotton fluff and talc powder+fluff into the peritoneal cavity respectively. In the ischemia group, we clamped the root of superior mesenteric artery for two minutes to induce ischemia on the small bowel of the rat. We counted the number of the peritoneal adhesive sites in peritoneal cavity on the 7th, 14th , 21st and 42nd postoperative day after peritoneal adhesion induced operation, and observed the characters of the adhesion state. The average number of the peritoneal adhesions were 1.25 per one rat in the talc powder group, 1.44 in the fluff group, 1.31 in the talc+fluff group and 1.31 in the ischemia group compared with 0.94 per one rat in the control group performed sham operation. 91.6% of the all adhesions were observed on the peritoneum of the previous incision sites and only 8.4% of the adhesions were adhered to the peritoneal surface of non-incision sites. The data of this study suggest that peritoneal injury and inflammatory reaction is the major cause of peritoneal adhesion. Also this study indicates that foreign bodies can develop peritoneal adhesion, but the effect is much lower than that of peritoneal injury with inflammatoty reaction.

사고양식을 통한 대학생의 비판적 사고 성향의 구인 특성 이해

강승희,김대현 경북대학교 사범대학부속 중등교육연구소 2006 중등교육연구 Vol.54 No.3