당의 종류 및 첨가량을 달리한 깨다식의 품질특성

김진숙,한영실,유선미,김행란,전혜경 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.3

깨다식의 감미 및 물성 등의 개선을 위해 꿀보다 높은 점도와 hardness를 가지는 쌀엿과 조청 등을 첨가 혼합하여 품질을 조사한 결과를 요약하면 다음과 같다. 깨다식의 적정 재료 배합비는 깨가루 100 기준시, 꿀과 쌀엿의 동량 혼합액 50%(w/w)였으며, 이를 기준으로 제조한 깨다식의 이화학적 특성은 당도 17.44 brix˚, tnqns 12.16, 환원당 15.44, 단백질 12.88, 지방 32.02, 조회분 2.82 %이었고, 기계적 특성 중 TPA의 chewiness는 0.28~0.30, hardness는 27.22~27.44 g/㎠이고, 색도의 L값은 41.67, a값 4.58, 그리고 b값은 16.99였다. This study was carried out to investigate the effects of the addition of sweeteners to Sesame Dasiks. Sesame Dasiks were prepared by adding various sweeteners to sesame seeds powder, at 40, 50, 60 and 70%, and their quality characteristics were measured. In the chewiness and hardness analyses of Sesame Dasiks, the 50% group (the same ratio and mixture of honey and lire jelIy) gave the highest results. For the sweetness, taste and overall acceptability of the Sesame Dasiks, the 50% group was also the better The texture profile analyses found the optimal chewiness and hardness of the Sesame Dasik to be 0.28 ~ 0.30 and 27.22 ~ 27.44, respectively.

갑상선 유두암이 병발된 가족성 대장 용종증 1예

백종현,소창배,이은영,이지숙,신은경,박은호,서정아,양재홍,송준영,박선자,박요한,최영식 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Familial adenomatous polyposis (FAP) is an autosomal dominant syndrome, typically characterized by multiple colorectal adenomas and increased incidence of colorectal carcinomas if it is not treated. Moreover, a variety of extracolonic manifestation are seen. The prevalence of thyroid tumors developing in patients with FAP is about 1∼2%. Generally, papillary thyroid cancer indicates low mortality but ,however, as people get older, the cancer becomes more aggressive. So, the operation is required. Recently we experienced a case of FAP, presenting with papillary thyroid carcinoma, and reported with a brief review of literatures.

Plasma Osteopontin Is a Useful Diagnostic Biomarker for Advanced Non-Small Cell Lung Cancer

Han, Seon-Sook,Lee, Seung-Joon,Kim, Woo Jin,Ryu, Dong Ryeol,Won, Jun Yeon,Park, Shinyoung,Cheon, Myeong Ju The Korean Academy of Tuberculosis and Respiratory 2013 Tuberculosis and Respiratory Diseases Vol.75 No.3

Background: Osteopontin (OPN) and carbonic anhydrase IX (CAIX), which are expressed on the surface of tumor cells, are associated with hypoxia during tumor development and progression. However, the roles of these proteins in the plasma of patients with non-small cell lung cancer (NSCLC) are poorly understood. Herein, we hypothesized that plasma OPN and CAIX levels could be used as diagnostic and prognostic tumor markers in patients with NSCLC. Methods: Fifty-three patients with NSCLC and 50 healthy control subjects were enrolled. We selected controls without malignancy and matched them with NSCLC patient cases according to age and gender. Blood samples were collected at the time of diagnosis; the plasma levels of OPN and CAIX were measured by enzyme-linked immunosorbent assays. Results: The plasma levels of OPN in the patients with NSCLC were significantly elevated as compared to those in the controls (p=0.016). However, there was no difference in the plasma level of CAIX between the NSCLC patients and controls. NSCLC patients with a distant metastasis had a remarkable increase in plasma OPN compared with patients without metastasis (p=0.026), but no such correlation was found for CAIX. There was no difference in overall survival rates according to the plasma level of OPN between the two groups (by Kaplan-Meier survival analysis). Conclusion: Plasma OPN levels were elevated in patients with NSCLC as compared with the controls, with greater elevation of OPN levels in the advanced stages of disease. Therefore, plasma OPN may have utility as a diagnostic, but not prognostic, biomarker of advanced NSCLC.

Degradation of 3-Methyl-4-nitrophenol, a Main Product of the Insecticide Fenitrothion, by Burkholderia sp. SH-1 Isolated from Earthworm (Eisenia fetida) Intestine

Seon Hwa Kim,Myung Ryeol Park,Song Ih Han,Kyung Sook Whang,Jae Han Shim,In Seon Kim 한국응용생명화학회 2007 Journal of Applied Biological Chemistry (J. Appl. Vol.50 No.4

Plasma Osteopontin Is a Useful Diagnostic Biomarker for Advanced Non-Small Cell Lung Cancer

( Seon Sook Han ),( Seung Joon Lee ),( Woo Jin Kim ),( Dong Ryeol Ryu ),( Jun Yeon Won ),( Shin Young Park ),( Myeong Ju Cheon ) 대한결핵 및 호흡기학회 2013 Tuberculosis and Respiratory Diseases Vol.75 No.3

Background: Osteopontin (OPN) and carbonic anhydrase IX (CAIX), which are expressed on the surface of tumor cells, are associated with hypoxia during tumor development and progression. However, the roles of these proteins in the plasma of patients with non-small cell lung cancer (NSCLC) are poorly understood. Herein, we hypothesized that plasma OPN and CAIX levels could be used as diagnostic and prognostic tumor markers in patients with NSCLC. Methods: Fifty-three patients with NSCLC and 50 healthy control subjects were enrolled. We selected controls without malignancy and matched them with NSCLC patient cases according to age and gender. Blood samples were collected at the time of diagnosis; the plasma levels of OPN and CAIX were measured by enzyme-linked immunosorbent assays. Results: The plasma levels of OPN in the patients with NSCLC were significantly elevated as compared to those in the controls (p=0.016). However, there was no difference in the plasma level of CAIX between the NSCLC patients and controls. NSCLC patients with a distant metastasis had a remarkable increase in plasma OPN compared with patients without metastasis (p=0.026), but no such correlation was found for CAIX. There was no difference in overall survival rates according to the plasma level of OPN between the two groups (by Kaplan-Meier survival analysis). Conclusion: Plasma OPN levels were elevated in patients with NSCLC as compared with the controls, with greater elevation of OPN levels in the advanced stages of disease. Therefore, plasma OPN may have utility as a diagnostic, but not prognostic, biomarker of advanced NSCLC.

cDNA Cloning and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes-Jocheon

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Fungi belonging to the Paecilomyces spp. have recently been used as food and herbal medicines in Korea and are greatly popular as commercially available powdered supplement or dried fruiting body. Despite this acceptance and its use, little is known of the genes related to its reactive agents. Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces spp. based on the previous identification of ITS1 and ITS2 at the molecular level and collected from Jocheon Miryang, Korea. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes- Jocheon was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDH is comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA. More investigation works including gene expression, immunological analysis etc. will be carried continuously without hesitation after this presentation.

Degradation of 3-Methyl-4-nitrophenol, a Main Product of the Insecticide Fenitrothion, by Burkholderia sp. SH-1 Isolated from Earthworm (Eisenia fetida) Intestine

Kim, Seon-Hwa,Park, Myung-Ryeol,Han, Song-Ih,Whang, Kyung-Sook,Shim, Jae-Han,Kim, In-Seon The Korean Society for Applied Biological Chemistr 2007 Journal of Applied Biological Chemistry (J. Appl. Vol.50 No.4

Microorganisms were isolated from earthworm intestine and examined for their ability to degrade 3-methyl-4-nitrophenol (MNP), a main degradation product of the insecticide fenitrothion. An isolate that showed the best degradation of MNP was selected for further study. The 16S rRNA analysis showed that the isolate belongs to the genus of Burkholderia, close to phenanthrene-degrading Burkholderia sp. S4.9, and is named Burkholderia sp. SH-1. When time-course degradation of MNP by SH-1 was examined by high performance liquid chromatographic analysis, almost complete degradation of MNP was observed within 26 h. Colony forming unit value assays indicated that the isolate SH-1 was capable of utilizing MNP as a sole carbon source. SH-1 could also degrade p-nitrophenol (PNP) but could not degrade ortho-substituted nitroaromatics such as 2,4-, 2,6- and 2,5-dinitrophenol. Catechol was detected as the main degration product of MNP and PNP. SH-1 was also found in the soil from which earthworms were obtained. These results suggest that the dispersal of Burkholderia sp. SH-1 into different environment with the aid of earthworms is likely to play a role in bioremediation of the soil contaminated with MNP.

Effect of Dongchunghacho on Gene Expression in Aorta and Liver in ApoE -/- Mice Fed a High-fat/high-cholesterol Diet

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sook Jae Sea,Hong Ja Kim,Eunju Park,Gyeong Im Jeon,Hyun Jung Lee, 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

A full genomic DNA microarray technique was employed to investigate the effects of Dongchunghacho on aortal and hepatic gene expression in apolipoprotein E knockout mice fed a high-fat/high-cholesterol diet. Male 8- week - old ApoE-/- mice were randomly divided into two groups, control(high cholesterol group; HC) and supplementation of Dongchunghacho (SD). All of the mice were fed a high-fet/high cholesterol diet with or without Dongchunghacho supplemented by 1% for 6 weeks. At first, lipid profile of the Dongchunghacho was measured by biochemical analysis. No differences were observed in serum triglyceride and total cholesterol levels between the two groups. Antigenotoxic effect of the Dongchunghacho was measured by the single cell gel electrophoresis assay (Comet assay) and quantified as % fluorescence in tail. Dongchunghacho supplementation decreased significantly leukocytic DNA damage and also there was a tendency of reduction in hepatic DNA damage in Dongchunghacho group compared with the control group. In up regulated genes in liver and aorta of the mice, genes with 0 to 2- fold difference in expression level between the two group (HD and SD) was very much more in liver than in aorta, on the contrary, those with 2-fold to 16-flod difference increased greatly rather in aorta than in liver. Also, almost the same results were observed in down regulated genes in liver and aorta between the two groups. These results suggested that supplementation of Dongchunghacho might be helpful in preventing leukocytic DNA damage induced by high fat diet, and has a more crucial roles in aortal gene expression.

Molecular Cloning, Expression and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes Jocheon-1

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces tenuipes Jocheon-1. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. Paecilomyces tenuipes Jocheon-1 cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes Jocheon-1 was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDHis comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The cDNA encoding Pt-GAPDH was expressed as a 37 kDa polypeptide in baculovirus-infected insect Sf9 cells. The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA.

MUC5AC 프로모터의 유전자 다형성과 천식과의 연관성

한선숙 ( Seon Sook Han ),성지현 ( Ji Hyun Sung ),이미은 ( Mi Eun Lee ),이승준 ( Seung Joon Lee ),이성준 ( Sung Joon Lee ),김우진 ( Woo Jin Kim ) 대한결핵 및 호흡기학회 2007 Tuberculosis and Respiratory Diseases Vol.63 No.3

연구배경: 기관지 점액의 과분비는 천식의 중요한 기전중의 하나이며, 특히천식 환자에서는 MUC5AC가 중요한 역할을 하는 것으로 알려져 있다. MUC5AC는 다양한 유전자 다형성을 갖는 것으로 알려져 있으나 MUC5AC 유전자 다형성과 천식과의 관계를 본 연구는 거의 없는 실정이다. 따라서 본 연구는 MUC5AC 프로모터 부위의 유전자 다형성과 천식과의 관계를 조사하였다. 방법: 강원대학교 병원에서 78명의 천식환자와 이들과 성별, 나이가 일치하는 78명의 대조군을 선정하였다. 이들로부터 전혈을 채취하여 DNA를 분리하여 PCR과 RFLP를 이용하여 MUC5AC 프로모터의 -1274G>A 유전자 다형성을 분석하였다. 모든 대상환자는 의무기록지를 검토하여 주된 증상과 투여 약제를 확인하였으며, 이들에서의 폐기능, 총 호산구수, 혈청 IgE, 피부반응검사 결과를 조사하였다. 결과: 천식환자의 평균 나이는 47.7±16.1세, 남자가 38.5%이었으며, 평균 FEV1은 84.4±22.3%이었다. MUC5AC 프로모터의 -1274G>A 유전자 다형성은 두 군간에 차이가 없었다(p=0.752, Cod). 천식 증상의 심한 정도, FEV1, 총 호산구수, 혈청 IgE, PC20, 아토피의 유무에 따라서도 MUC5AC 프로모터의 -1274G>A 유전자 다형성은 차이가 없었다. 결론: MUC5AC 프로모터의 -1274G>A 유전자 다형성은 천식과는 무관하였으며, 여러 가지 임상적인 지표들과도 상관관계를 보이지 않았다. Background: Airway mucus hypersecretion plays an important role in the pathogenesis of asthma, and is associated with the induction of MUC5AC expression in airway secretion. The MUC5AC gene is highly polymorphic; however, there are few studies about the association between the polymorphisms of the MUC5AC gene and asthma susceptibility or asthma phenotypes. We have investigated the association of MUC5AC promoter polymorphisms with the risk of asthma and asthma phenotypes. Methods: We determined the genotypes of the MUC5AC promoter (-1274G>A) in 78 asthma patients and in 78 age, sex-matched control individuals in the Korean population. Genomic DNAs from blood were analyzed by PCR and RFLP (restriction fragment length polymorphism) determination. We examined FEV1, total eosinophil count, serum IgE level, PC20 and the presence of atopy (by a skin test) in asthma patients. Results: The mean age of the patients was 47.7 ± 16.1 years and 38.5% were men, and the mean FEV1 was 84.4 ± 22.3% of predicted in the asthma patients. The -1274G>A polymorphism of the MUC5AC promoter in asthma patients was not significantly different as compared with normal individuals (GG 57.7%, AG 34.6% and AA 7.7% in asthma patients vs. GG 56.4%, AG 38.5% and AA 5.1% in control subject, p = 0.752, Cod). Several clinical parameters in asthma patients such as FEV1, total eosinophil count, serum IgE level, PC20 and the presence of atopy, were not associated with the -1274G>A polymorphism of the MUC5AC promoter. Conclusion: The -1274G>A single nucleotide polymorphism (SNP) frequency of the MUC5AC promoter was not associated with asthma in a Korean population. (Tuberc Respir Dis 2007; 63: 235-241)