http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Hyeong Seon Lee ),( Gyeong Seon Lee ),( Seon Hee Kim ),( Hyun Kyung Kim ),( Dong Hee Suk ),( Dong Seok Lee ) 생화학분자생물학회 2014 BMB Reports Vol.47 No.2
Orostachys japonicus shows various biological activities. However, the molecular mechanisms remain unknown in LPS-stimulated macrophages. Here, we investigated the anti-oxidizing effect of the dichloromethane (DCM) and hexane fractions from O. japonicus (OJD and OJH) against oxidative stress in RAW 264.7 cells stimulated by LPS. OJD and OJH significantly increased the expression of heme oxygenase-1 (HO-1) in a dose- and time-dependent manner. Additionally, it was found that the expression of HO-1 was stimulated by Nrf2 activated via degradation of Keap1. ERK and p38 inhibitors repressed HO-1 induced by OJD and OJH in LPS-stimulated cells, respectively. In conclusion, these results suggest that OJD and OJH may block oxidative damage stimulated by LPS, via increasing the expression of HO-1 and Nrf2, and MAPK signaling pathway. [BMB Reports 2014; 47(2): 98-103]
A Repeated-dose Oral Toxicity Study of Orostachys japonicus Extract in Sprague-Dawley Rats
Deok-Seon Ryu,Mi-Young Lee,Hyeong-Seon Lee,Seon-Hee Kim,Gyeong-Seon Lee,Ji-Hye Kwon,Dong-Seok Lee 대한의생명과학회 2012 Biomedical Science Letters Vol.18 No.1
A 12-week repeated-dose oral toxicity study of water-soluble Orostachys japonicus extract (WOJ) was performed in Sprague-Dawley (SD) rats of both genders. Each group of ten rats was orally administered in doses of either 0 or 250 mg/day over a 12-week period. As a result, no WOJ-related changes were observed in terms of survival rate, clinical signs, body weight, or food intake. In addition, no difference in organ weight between the control and treated groups was detected. Furthermore, serum biochemistry parameters revealed some changes within normal ranges although significant decreases in total-bilirubin in the females. In spite of some alterations in serum biochemistry, the clinical signs, body weight changes from food intake, and autoptical remarks indicated that WOJ was not toxic. This study suggests that repeated treatment of O. japonicus very low toxicity and the NOAEL (no observed adverse effect dose) of WOJ exceeds 250 mg/kg in the SD rats.
박선경(Seon Gyeong Bak),임형진(Hyung Jin Lim),원영선(Yeong-Seon Won),박은재(Eun Jae Park),오제훈(Je Hun Oh),김지은(Ji Eun Kim),이민지(Min Jee Lee),이소영(Soyoung Lee),이승웅(Seung Woong Lee),이승재(Seung Jae Lee),노문철(Mun Chual Rho 한국축산식품학회 2023 Food and Life Vol.2023 No.1
The skin is the largest organ that blocks invasion to the outside. Due to external stimuli, the skin barrier collapses and immune function abnormalities occur. This leads to skin diseases such as atopic dermatitis and psoriasis. Through this study, we attempted to develop a natural material that can be used for various dermatitis diseases. In this study, the gene expression of skin barrier-related proteins was confirmed using tumor necrosis factor-α/interferon-γ-stimulated HaCaT cells through real-time PCR. As a result, it was confirmed that the gene expression of skin barrier-related proteins and ceramide-related proteins was improved by the ABE-M. In this study, it was confirmed that the gene expression of filaggrin, involucrin, and loricrin, which are skin barrier-related proteins, was improved by ABE-M, and it was confirmed that the gene expression of the tight junction-related proteins, occludin and zonula occludens proteins-1, was also increased. As such, it is expected that ABE-M can be used as a material for skin inflammation such as atopic dermatitis.
( Seon-jin Lee ),( Tae Woo Kim ),( Gyeong Lim Park ),( Yo Sep Hwang ),( Hee Jun Cho ),( Jong-tae Kim ),( Hee Gu Lee ) 생화학분자생물학회(구 한국생화학분자생물학회) 2019 BMB Reports Vol.52 No.11
G protein-coupled estrogen receptor (GPER) is known to play an important role in hormone-associated cancers. G-1, a novel synthetic GPER agonist, has been reported to exhibit anti-carcinogenic properties. However, the chemotherapeutic mechanism of GPER is yet unclear. Here, we evaluated GPER expression in human gastric cancer tissues and cells. We found that G-1 treatment attenuates GPER expression in gastric cancer. GPER expression increased G-1-induced antitumor effects in mouse xenograft model. We analyzed the effects of knockdown/overexpression of GPER on G-1-induced cell death in cancer cells. Increased GPER expression in human gastric cancer cells increased G-1-induced cell death via increased levels of cleaved caspase-3, -9, and cleaved poly ADP-ribose polymerase. Interestingly, during G-1-induced cell death, GPER mRNA and protein expression was attenuated and associated with ER stress-induced expression of PERK, ATF-4, GRP-78, and CHOP. Furthermore, PERK-dependent induction of ER stress activation increased G-1-induced cell death, whereas PERK silencing decreased cell death and increased drug sensitivity. Taken together, the data suggest that the induction of ER stress via GPER expression may increase G-1-induced cell death in gastric cancer cells. These results may contribute to a new paradigm shift in gastric cancer therapy. [BMB Reports 2019; 52(11): 647-652]
Lee, Eun-Gyeong,Yoon, Sang-Hwal,Das, Amitabha,Lee, Sook-Hee,Li, Cui,Kim, Jae-Yean,Choi, Myung-Suk,Oh, Deok-Kun,Kim, Seon-Won Wiley Subscription Services, Inc., A Wiley Company 2009 Biotechnology and bioengineering Vol.102 No.1
<P>The amplification of gltA gene encoding citrate synthase of TCA cycle was required for the efficient conversion of acetyl-CoA, generated during vanillin production from ferulic acid, to CoA, which is essential for vanillin production. Vanillin of 1.98 g/L was produced from the E. coli DH5α (pTAHEF-gltA) with gltA amplification in 48 h of culture at 3.0 g/L of ferulic acid, which was about twofold higher than the vanillin production of 0.91 g/L obtained by the E. coli DH5α (pTAHEF) without gltA amplification. The icdA gene encoding isocitrate dehydrogenase of TCA cycle was deleted to make the vanillin producing E. coli utilize glyoxylate bypass which enables more efficient conversion of acetyl-CoA to CoA in comparison with TCA cycle. The production of vanillin by the icdA null mutant of E. coli BW25113 harboring pTAHEF was enhanced by 2.6 times. The gltA amplification of the glyoxylate bypass in the icdA null mutant remarkably increased the production rate of vanillin with a little increase in the amount of vanillin production. The real synergistic effect of gltA amplification and icdA deletion was observed with use of XAD-2 resin reducing the toxicity of vanillin produced during culture. Vanillin of 5.14 g/L was produced in 24 h of the culture with molar conversion yield of 86.6%, which is the highest so far in vanillin production from ferulic acid using recombinant E. coli. Biotechnol. Bioeng. 2009;102: 200–208. © 2008 Wiley Periodicals, Inc.</P>
( Gyeong Been Lee ),( Jienny Lee ),( Yong Woo Sohn ),( Na Yeon Gu ),( Hee Ryang Kim ),( Jeong Su Byeon ),( Hyung Seon Jeon ),( Jong Duck Jang ),( Young Jin Yang ),( In Soo Cho ),( Sang Ho Cha ) 한국예방수의학회(구 한국수의공중보건학회) 2015 예방수의학회지 Vol.39 No.3
Bone fractures are most often seen in racetrack horses because of the high level of intensity in racing. These issues are the main cause of decreased performance in racehorses. Mesenchymal stem cells (MSCs) have been explored to improve intra-articular therapy in racehorses. MSCs are essential for the repair and regeneration of damaged tissues. In this study, the effect of intra-articular injection of MSCs in racehorses was investigated. Before accessing the MSC therapy, synovial fluids were obtained from the fracture site of racehorses, and adipose tissue was collected for MSC isolation. Using the MSC specific marker, adipose tissue-derived MSCs were identified. The racehorses received intra-articular injection of autologous MSCs (or allogeneic) (3 × 107 cells/3 mL). After 1 or 2 weeks, synovial fluids were collected from racehorses. To test the effect of MSC injection using ELISA, we analyzed inflammatory factors from the untreated samples compared to MSC-treated samples of racehorses. The level of pro-inflammatory factors (interleukin-1β and prostaglandin E2) was significantly decreased in synovial fluids of MSC-injected racehorses, compared to before accessing the MSC therapy, whereas, the level of anti-inflammatory factor (interleukin-10) was higher than prior to accessing the MSC therapy. Further studies are needed to investigate the anti-inflammatory mechanism of MSC in racehorses.
Tissue-specific DNA damage response in Mouse Whole-body irradiation
Lee Seon-Gyeong,Kim Namwoo,박인배,Park Jun Hong,Myung Kyungjae 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.1
Background Genomic instability is a hallmark of various cancers, and DNA repair is an essential process for maintaining genomic integrity. Mammalian cells have developed various DNA repair mechanisms in response to DNA damage. Compared to the cellular response to DNA damage, the in vivo DNA damage response (DDR) of specific tissues has not been studied extensively. Objective In this study, mice were exposed to whole-body gamma (γ)-irradiation to evaluate the specific DDR of various tissues. We treated male C57BL6/J mice with γ-irradiation at different doses, and the DDR protein levels in different tissues were analyzed. Results The level of gamma-H2A histone family member X (γH2AX) increased in most organs after exposure to γ-irradiation. In particular, the liver, lung, and kidney tissues showed higher γH2AX induction upon DNA damage, compared to that in the brain, muscle, and testis tissues. RAD51 was highly expressed in the testis, irrespective of irradiation. The levels of proliferating cell nuclear antigen (PCNA) and ubiquitinated PCNA increased in lung tissues upon irradiation, suggesting that the post-replication repair may mainly operate in the lungs in response to γ-irradiation. Conclusion These results suggest that each tissue has a preferable repair mechanism in response to γ-irradiation. Therefore, the understanding and application of tissue-specific DNA damage responses could improve the clinical approach of radiotherapy for treating specific cancers.