경부에 발생한 2새열낭종 2례

박홍주,박세찬,손영휘,윤천주,유선열 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.4

Developmental anomalies arising from the branchial apparatus include cysts, external sinuses, internal sinuses, and complete fistulas. Second branchial cleft cysts are by far the most common among these anomalies. It may occur at any age, being most common in the third decade, and more frequent in the male than in the female. It usually presents a smooth, round, nontender fluctuant mass located between the level of the tragus and the clavicle along the anteromedial border of the sternocleidomastoid muscle. It is lined by respiratory or squamous epithelium unless inflammation is present. The considerable amount of lymphoid tissue may be found beneath the epithelium. The treatment of choice of branchial cleft cyst is surgical excision. If the lesion is acutely infected, however, it is essential to relieve the infection prior to the surgery This report deals with two cases of second branchial cleft cyst. In case 1, the cyst had rapidly increased in size over pregnant period. In case 2, the patient presented the swelling in the left neck, and had the history of incision and drainage because of misdiagnosis as submandibular space abscess. The infection was treated by antibiotic therapy in the first place, and then complete surgical excision was made. There was no evidence of any recurrence or complications for these 3∼4 years.

Anti-inflammatory Activity of Saxifragin via Inhibition of NF-κB Involves Caspase-1 Activation

Cheon, Se-Yun,Chung, Kyung-Sook,Jeon, Eunjin,Nugroho, Agung,Park, Hee-Jun,An, Hyo-Jin American Chemical Society and American Society of 2015 Journal of natural products Vol.78 No.7

<P>Saxifragin, the 5-glucoside of the flavonoid quercetin, is found in plants and insects. It has been reported that saxifragin has peroxynitrite-scavenging effects. However, the mechanism of anti-inflammatory effects of saxifragin has not yet been clearly identified. In this study, we investigated the anti-inflammatory effects of saxifragin in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and animal models of inflammation. We found that saxifragin suppressed the production of nitric oxide (NO) and prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) in LPS-activated RAW 264.7 macrophages by suppressing the level of protein and mRNA expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), respectively. Furthermore, saxifragin inhibited mRNA expression of pro-inflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β. We studied the inhibitory effects of saxifragin on the nuclear translocation of nuclear factor (NF)-κB, activation of caspase-1, and phosphorylation of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK). Furthermore, pretreatment with saxifragin increased the survival rate of mice with LPS-induced septic death. Collectively, these findings suggest that saxifragin exerts anti-inflammatory activity by inhibiting NF-κB, caspase-1, and mitogen-activated protein kinase (MAPK) activation.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2015/jnprdf.2015.78.issue-7/acs.jnatprod.5b00145/production/images/medium/np-2015-00145r_0008.gif'></P>

The Inhibitory Effect of Bee Venom on Mast Cell Mediated Allergic Inflammation Responses

Yun-Mi Kang,Se-Yun Cheon,Kyung-Sook Chung,Hyo-Jin An 한국식품영양과학회 2016 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Anti-inflammatory Effects of Ethanol Extract of Korean Medicinal Plants at Hwaak Mountain in LPS-induced RAW 264.7 Macrophages

( Yun-mi Kang ),( Eun-jin Jeon ),( Kyung-sook Chung ),( Se-yun Cheon ),( Jong Hyuk Park ),( Yoo-chang Han ),( Hyo-jin An ) 대한본초학회 2017 大韓本草學會誌 Vol.32 No.2

Objectives : This study was conducted to investigate candidate materials as anti-inflammatory agent from extracts of Korean medicinal plants in Hwaak mountain. Ligustrum obtusifolium (LO) is a Korea medicinal plants that commonly used for robustness and hemostasis. It has been reported that LO has exhibited anti-ischemic, anti-oxidative, antihypolipidemic, anti-tumor and hypoglycemic effects. However, LO has not been previously reported to have an antiinflammatory effect. Therefore, we have evaluated the anti-inflammatory effects of LO and its underlying molecular mechanisms in LPS-induced RAW 264.7 macrophages. Methods : Cell viability was determined by MTT assay in RAW 264.7 macrophages. Nitric Oxide (NO) was measured with Griess reagent and pro-inflammatory cytokines were detected by ELISA in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Protein expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and p65 subunit of nuclear factor-κB (NF-κB) were determined by Western blot analysis. Results : Among 15 extracts of Korean medicinal plants tested, Ligustrum obtusifolium (LO) showed the inhibition of NO production without cytotoxicity. LO reduced the expression levels of iNOS and COX-2 proteins in LPS-simulated RAW 264.7 macrophages in dose-dependent manner. Consistent with these data, LO inhibited the productions of TNF-α, IL-6, and IL-1β in LPS-simulated RAW 264.7 macrophages. Furthermore, LO attenuated the LPS-induced nuclear translocation of p65 NF-κB in RAW 264.7 macrophages involving suppression of NF-κB activation. Conclusions : Taken together, these results suggest that the anti-inflammatory effects of LO is associated with regulation of inflammatory mediators via inhibition of NF-κB activation in LPS-treated RAW 264.7 macrophages.

The Relevance of Serum Ghrelin Concentration to Severity of Acute Pancreatitis

( Se Hyung Lee ),( Young Don Kim ),( Yun Ho Kong ),( Koon Hee Han ),( Woo Jin Jeong ),( Sang Jin Lee ),( Gab Jin Cheon ) 대한소화기기능성질환·운동학회 2010 Gut and Liver Vol.4 No.2

Background/Aims: Ghrelin has recently been reported as exerting a protective effect in the damaged pancreas in rats. We investigated the correlation between severity of acute pancreatitis and serum ghrelin concentrations. Methods: Blood samples were collected three times (at admission, after 48 hours, and at discharge) from patients admitted with acute pancreatitis. We divided the patients into nonrisk and risk groups. The risk group was defined as the presence of at least one of following risk factors for severe acute pancreatitis: Ranson`s score ≥3, acute physiology and chronic health evaluation (APACHE) II score ≥8, C-reactive protein (CRP) ≥150 mg/L, and CT severity index (CTSI) ≥4. Serum ghrelin concentrations were measured with RIA kit and analyzed based on clinical and biochemical parameters. Results: A total of 53 patients was enrolled in this study: 28 in the nonrisk group and 25 in the risk group. At admission, the ghrelin concentration was significantly higher in the risk group (286.39±272.19 vs 175.96±138.87 pg/mL [mean±SD], p=0.049). However, the ghrelin concentration did not differ significantly between the two groups after 48 hours (p=0.450) and at discharge (p=0.678). The overall ghrelin concentration was significantly lower at admission than at discharge (240.65±247.96 vs 369.41±254.27 pg/mL, p=0.001). Conclusions: Patients with risk factors for severe acute pancreatitis have higher serum ghrelin concentrations. (Gut Liver 2010;4:234-240)

Redesign, Reconstruction, and Directed Extension of the Brevibacterium linens C40 Carotenoid Pathway in Escherichia coli

Kim, Se Hyeuk,Park, Yun Hee,Schmidt-Dannert, Claudia,Lee, Pyung Cheon American Society for Microbiology 2010 Applied and environmental microbiology Vol.76 No.15

<B>ABSTRACT</B><P>In this study, the carotenoid biosynthetic pathways of <I>Brevibacterium linens</I> DSMZ 20426 were reconstructed, redesigned, and extended with additional carotenoid-modifying enzymes of other sources in a heterologous host <I>Escherichia coli</I>. The modular lycopene pathway synthesized an unexpected carotenoid structure, 3,4-didehydrolycopene, as well as lycopene. Extension of the novel 3,4-didehydrolycopene pathway with the mutant <I>Pantoea</I> lycopene cyclase CrtY2 and the <I>Rhodobacter</I> spheroidene monooxygenase CrtA generated monocyclic torulene and acyclic oxocarotenoids, respectively. The reconstructed β-carotene pathway synthesized an unexpected 7,8-dihydro-β-carotene in addition to β-carotene. Extension of the β-carotene pathway with the <I>B. linens</I> β-ring desaturase CrtU and <I>Pantoea</I> β-carotene hydroxylase CrtZ generated asymmetric carotenoid agelaxanthin A, which had one aromatic ring at the one end of carotene backbone and one hydroxyl group at the other end, as well as aromatic carotenoid isorenieratene and dihydroxy carotenoid zeaxanthin. These results demonstrate that reconstruction of the biosynthetic pathways and extension with promiscuous enzymes in a heterologous host holds promise as a rational strategy for generating structurally diverse compounds that are hardly accessible in nature.</P>

Comparative analysis of expressed sequence tags (ESTs) between normal group and softness syndrome group in Halocynthia roretzi

정지은,Se-Won Kang,Yun Kyung Shin,Je Cheon Jun,Young-Ok Kim,Young Baek Hur,Jae-Hyung Kim,Sung-Hwa Chae,Jun-Sang Lee,최인호,Yeon Soo Han,석대현,이용석 대한독성 유전단백체 학회 2011 Molecular & cellular toxicology Vol.7 No.4

To identify the cause of mass mortality in ascidians, we constructed cDNA libraries of both the normal and softness syndrome group in Halocynthia roretzi. To perform comparative analysis of transcripts between the two groups, we sequenced about 1,000random clones. All the sequences obtained from the clones were processed to remove the vector region and low quality sequences through base calling and vector trimming. We collected 906 sequences with average length of 463 bp in the normal group and 1014 sequences in the softness syndrome group with an average length of 696 bp. Clustering and assembling of EST sequences using TGICL package resulted in 906 distinct sequences composed of 517 singletons and 77contigs in 75 clusters in normal group and 1014 distinct sequences composed of 707 singletons and 120contigs in 120 clusters in the softness syndrome group. All sequences derived from the two groups were compared against the NCBI Non-redundant database using BLASTX algorithms. As a result, 493 sequences in the normal group and 861 sequences in the softness syndrome group had significant hits within the database. In addition, we listed genes that showed differential expression in the softness syndrome group. Transcript levels of calponin increased by 11-fold and both E3ubiquitin-protein ligase MARCH3 and selenium dependent salivary glutathione peroxidase by 5-fold in the softness syndrome group. Also, the expression of four genes including muscle actin increased by 4-fold. In contrast, we observed down-regulation of genes encoding trypsinogen 1, cathepsin D protein, serine protease,and halocidin precursor, decreasing by more than 6-fold. Herein, we identified differential expression of genes involved in the contraction and regulation of muscle cells and immune reaction in H. roretzi with softness syndrome. This study is the first report on gene expression changes occurring in H. roretzi with softness syndrome and would be useful in further studies.

제언 - '건설기술개발'이 곧 경쟁력이다

천세윤,Cheon, Se-Yun 대한기계설비건설협회 2013 기계설비 Vol.274 No.-

해상크레인 윈치 감속기의 기계학습기반 실시간 상태모니터링 시스템 개발

황세윤(Se-Yun Hwang),이장현(Jang-Hyun Lee),김광식(Kwang-Sik Kim),오재원(Jae-Won Oh),민천홍(Cheon-Hong Min) (사)한국CDE학회 2020 한국CDE학회 논문집 Vol.25 No.4

This study introduces development examples of monitoring system for winch equipment, the main equipment of floating cranes. The detail process was introduced to develop a system that can acquire sensor data in real time, monitor operating conditions and fault diagnosis. The proposed monitoring system is designed for winch equipment, which is a key equipment of the offshore crane. The system was developed for bearing part, which frequently causes failures in the winch equipment. In addition, we would like to introduce a relatively low-cost H/W configuration to facilitate application in small and medium-sized industries. The monitoring methods have been implemented by applying the method of Naïve Bayes classification based on the method of supervised learning.

Anti-inflammatory Effects of Ethanol Extract of Korean Medicinal Plants at Hwaak Mountain in LPS-induced RAW 264.7 Macrophages

Kang, Yun-Mi,Jeon, Eun-jin,Chung, Kyung-Sook,Cheon, Se-Yun,Park, Jong Hyuk,Han, Yoo-Chang,An, Hyo-Jin The Korea Association of Herbology 2017 大韓本草學會誌 Vol.32 No.2

Objectives : This study was conducted to investigate candidate materials as anti-inflammatory agent from extracts of Korean medicinal plants in Hwaak mountain. Ligustrum obtusifolium (LO) is a Korea medicinal plants that commonly used for robustness and hemostasis. It has been reported that LO has exhibited anti-ischemic, anti-oxidative, anti-hypolipidemic, anti-tumor and hypoglycemic effects. However, LO has not been previously reported to have an anti-inflammatory effect. Therefore, we have evaluated the anti-inflammatory effects of LO and its underlying molecular mechanisms in LPS-induced RAW 264.7 macrophages. Methods : Cell viability was determined by MTT assay in RAW 264.7 macrophages. Nitric Oxide (NO) was measured with Griess reagent and pro-inflammatory cytokines were detected by ELISA in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Protein expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and p65 subunit of nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) were determined by Western blot analysis. Results : Among 15 extracts of Korean medicinal plants tested, Ligustrum obtusifolium (LO) showed the inhibition of NO production without cytotoxicity. LO reduced the expression levels of iNOS and COX-2 proteins in LPS-simulated RAW 264.7 macrophages in dose-dependent manner. Consistent with these data, LO inhibited the productions of $TNF-{\alpha}$, IL-6, and $IL-1{\beta}$ in LPS-simulated RAW 264.7 macrophages. Furthermore, LO attenuated the LPS-induced nuclear translocation of p65 $NF-{\kappa}B$ in RAW 264.7 macrophages involving suppression of $NF-{\kappa}B$ activation. Conclusions : Taken together, these results suggest that the anti-inflammatory effects of LO is associated with regulation of inflammatory mediators via inhibition of $NF-{\kappa}B$ activation in LPS-treated RAW 264.7 macrophages.