Inhibition of p38 pathway-dependent MPTP-induced dopminergic neurodegeneration in estrogen receptor alpha knockout mice

( Chul Ju Hwang ),( Dong Young Choi ),( Yu Yeon Jung ),( Young Jung Lee ),( Jae Suk Yun ),( Ki Wan Oh ),( Sang Bae Han ),( Seikwan Oh ),( Mi Hee Park ),( Jin Tae Hong ) 영남대학교 약품개발연구소 2016 영남대학교 약품개발연구소 연구업적집 Vol.26 No.-

Approximately, 7-10 million people in the world suffer from Parkinson``s disease (PO), Recently, increasing evi-dence has suggested the protective effect of estrogens against nigrostriatal dopaminergic damage in PD. In this study, we investigated whether estrogen affects l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP)-induced behavioral impairment in estrogen receptor alpha (ERa)-deficient mice. MPTP (15 mg/kg, four times with l.5-h interval)-induced dopaminergic neurodegeneration was evaluated in ERα wild-type (WT) and knockout (KO) mice. Larger dopamine depletion, behavioral impairments (Rotarod test, Pole test, and Gait test), activation of mi-croglia and astrocytes, and neurointlammation after MPTP injection were observed in ERα KO mice compared to those in WT mice.Immunostaining for tyrosine hydroxylase (TH) after MPTP injection showed fewerTH-positive neurons in ERα KO mice than WT mice. Levels of dopamine and 3,4-<lihydroxyphenylacetic acid (OOPAC, metab-olite of dopamine) were also lowered in ERα KO mice after MPTP injection. Interestingly, a higher immunoreac-tivity for monoamine oxidase (MAO) B was found in the substantia nigra and striatum of ERα KO mice after MPTP injection. We also found an increased activation of p38 kinase (which positively regulates MAO B expression) in ERα KO mice. In vitro estrogen treatment inhibited neuroinflammation in l-methyl-4-phenyl pyridium (MPP +)-treated cultured astrocyte cells; however, these inhibitory effects were removed by p38 inhibitor. These results indicate that ERα might be important for dopaminergic neuronal survival through inhibition of p38 pathway.

연구논문 : 과학영재교육원 학생들의 과학 통합 탐구 능력

정은영 ( Eun Young Jeong ),권이영 ( Yi Young Kwon ),양주성 ( Joo Sung Yang ),고유미 ( Yu Mi Ko ) 경북대학교 과학교육연구소 2013 科學敎育硏究誌 Vol.37 No.3

과학영재교육원 학생들이 성공적으로 과학 탐구를 수행하기 위해서는 문제 인식, 가설 설정, 탐구 설계, 결론 도출 등의 과학 통합 탐구 능력이 요구된다. 이 연구에서는 김유향(2013)이 개발한 ‘과학 탐구 사고력 측정을 위한 서술형 평가도구’를 사용하여 과학영재교육원 학생들의 과학 통합 탐구 능력을 평가 요소별, 성별, 이수 과정별, 전공 분야별로 비교하였다. 이 평가도구는 탐구 사고력의 주요 요소들인 탐구 문제 도출, 변인 추출, 가설 설정, 실험 설계, 자료 변환, 자료 해석, 결론 도출, 일반화, 평가에 관한 문항이 각 2개씩 총 18문항으로 구성되어 있다. 이 평가도구를 2013년 현재 C 대학교 부설 과학영재교육원에 다니고 있는 학생들 중 102명 학생들(초등과학반 15명, 중등과학심화Ⅰ반 58명, 중등과학심화Ⅱ반 29명)을 대상으로 시행하였다. 그 결과, 과학 통합 탐구 능력의 요소 중 가설 설정, 변인 추출의 점수는 상대적으로 높았고, 자료 변환, 자료 해석, 평가의 점수는 상대적으로 낮았다. ‘자료 변환’에 해당하는 선 그래프 그리기 문항과 ‘평가’에 해당하는 실험 설계의 개선점 찾기 문항의 정답률은 약 40 %로 상대적으로 낮게 나타 났다. 영재교육을 받은 단계별로 비교하면 과학 통합 탐구 능력의 요소들 중 일반화의 경우 유의미한 차이가 있었다. 남학생과 여학생의 점수 차이는 유의미하지 않았다. 이 연구에서 사용한 평가도구가 생물 분야의 소재를 다루고 있어 생물 분야의 영재 학생들과 다른 분야(물리, 화학, 지구과학)의 영재 학생들과 비교한 결과, 유의미한 차이가 없었다. 과학영재교육원 학생들의 과학 통합 탐구 능력 향상을 위하여 자료 변환, 자료 해석, 평가에 관한 교수·학습을 강화하고, 과학 탐구와 관련된 구체적인 교수·학습 자료를 개발할 필요가 있다. The purpose of this study was to investigate science integrated process skill of the students in science education center for the gifted. In order to do this, ``free-response test for the assessment of science process skills`` developed by Yu-Hyang Kim(2013) was administered to 102 students(15 in elementary school science class, 58 in middle school science classⅠ, and 29 in middle school science classⅡ) who attend the program of science education center for the gifted in C university. The assessment tool measured 9 skills ; formulating inquiry questions, recognizing variables, formulating hypotheses, designing experiment, transforming data, interpreting data, drawing conclusions, formulating generalizations, and evaluating the designed experiments. As a result, the students in science education center for the gifted had relatively high scores in the area of ``formulating hypotheses`` and ``recognizing variables``, but they had relatively low scores in the area of ``transforming data``, ``interpreting data``, and ``evaluating the designed experiments``. The 2 items` percentage of correct answers were below 40% ; one is about a drawing a line graph in ``transforming data``, and the other requires finding improvements of the experimental design in ``evaluation``. There was no significant difference between boys`` scores and girls`s one, and between the scores of students in the field of biology and those of students in the other fields(physics, chemistry, and earth science) in science integrated process skills. And there was significant difference according to the periods receiving the gifted education in ``formulating generalizations``. The teaching and learning has to focus on improving science integrated process skills in the program of science education center for the gifted and teaching and learning materials needs to be developed.

A prospective association between dietary mushroom intake and the risk of type 2 diabetes: the Korean Genome and Epidemiology Study–Cardiovascular Disease Association Study

Yu-Mi Kim(Yu-Mi Kim),Hye Won Woo(Hye Won Woo),Min-Ho Shin(Min-Ho Shin),Sang Baek Koh(Sang Baek Koh),Hyeon Chang Kim(Hyeon Chang Kim),Mi Kyung Kim(Mi Kyung Kim) 한국역학회 2024 Epidemiology and Health Vol.46 No.-

Cerebral Metabolic Abnormalities in Children with Congenital Oculomotor Apraxia

( Sung June Jang ),( Yu Kyeong Kim ),( Yeong Mi Oh ),( Yoo Sung Song ),( Ji Soo Kim ),( Won Woo Lee ),( Sang Eun Kim ) 대한핵의학회 2007 핵의학 분자영상 Vol.41 No.5

Characteristics of the North Pacific Oscillation in CMIP5 models in relation to atmospheric mean states

Mi-Kyung Sung,Changhyun Yoo,Sang-Wook Yeh,Yu Kosaka,Soon-Il An 한국기상학회 2019 한국기상학회 학술대회 논문집 Vol.2019 No.10

Behaviour alteration of Topoisomerase IIIβ knockout mice

Yu Rim Kim,Eun Kyoung Kim,Young Sub Byun,Hae Rim Kim,Ju Sung Kang,Ju Young Lee,Min Young Lee,Sang Mi Cho,Ki Hoon Lee,Hoyoung Ghang,Won Kee Yoon,Young Suk Won,Hyoung Chin Kim,Young Hee Lee,Ki Hoan Nam 한국실험동물학회 2015 한국실험동물학회 학술발표대회 논문집 Vol.2015 No.8

Annual Change in Pulmonary Function and Clinical Characteristics of Combined Pulmonary Fibrosis and Emphysema and Idiopathic Pulmonary Fibrosis: Over a 3-Year Follow-up

( Yu Jin Kim ),( Seong Hyun Shin ),( Jeong Woong Park ),( Sun Young Kyung ),( Shin Myung Kang ),( Sang Pyo Lee ),( Yon Mi Sung ),( Yoon Kyung Kim ),( Sung Hwan Jeong ) 대한결핵 및 호흡기학회 2014 Tuberculosis and Respiratory Diseases Vol.77 No.1

Background: Combined pulmonary fibrosis and emphysema (CPFE) have different pulmonary function tests (PFTs) and outcomes than idiopathic pulmonary fibrosis (IPF). The intention of this study was to identify unknown differences between CPFE and IPF by a retrospective comparison of clinical data including baseline and annual changes in pulmonary function, comorbidities, laboratory findings, clinical characteristics and cause of hospitalization. Methods: This study retrospectively enrolled patients with CPFE and IPF who had undergone PFTs once or several times per year during a follow-up period of three years. Baseline clinical characteristics and the annual changes in the pulmonary function during the follow-up period were compared between 26 with CPFE and 42 patients with IPF. Results: The baseline ratio of forced expiratory volume in one second to forced vital capacity (FEV1/FVC%) in patients with CPFE was lower than that in patients with IPF (78.6±1.7 vs. 82.9±1.1, p=0.041). The annual decrease in FEV1/FVC in the CPFE was significantly higher than in the IPF. The annual decreases in diffusion capacity of carbon monoxide and FVC showed no significant differences between the two groups. The symptom durations of cough and sputum were in the CPFE significantly lower than in the IPF. The serum erythrocyte sedimentation rate level at the acute stage was significantly higher than in the IPF. There were no significant differences in the hospitalization rate and pneumonia was the most common cause of hospitalization in both study groups. Conclusion: The annual decrease of FEV1/FVC was in patients with CPFE significantly higher than in the patients with IPF.

싱잉볼 자애 명상 프로그램이 성인들의 생활스트레스와 뇌파에 미치는 효과

유미령(Mi-Ryoung Yu),윤상일(Sang-Il Youn),원희욱(Hee-Wook Weon) 한국산학기술학회 2023 한국산학기술학회논문지 Vol.24 No.3

본 연구는 싱잉볼 자애 명상 프로그램이 성인들의 생활스트레스와 뇌파에 미치는 효과를 분석하였다. 40세 이상 64세 미만의 성인들 중 남성 2명과, 여성 5명이 자발적으로 참여하였다. 중재 기간은 2022년 8월 7일부터 동년 8월 13일까지 7일간 60분씩 참여하였다. 회기마다 도입 20분 ‘자기성찰’, 본론 30분 ‘싱잉볼 자애 명상’, 마무리 10분 ‘나누기’로 구성되어 있다. 구조화된 설문지를 통해 일반적인 특성, 생활스트레스를 평가하고 뇌파를 측정하였다. 수집된 자료는 SPSS/WIN 25.0 프로그램으로 인구 사회학적 특성 파악을 위한 빈도분석, Wilcoxon 부호순위검정을 통하여 검증하였다. 그 결과 싱잉볼 자애 명상 프로그램이 생활스트레스 하위변인 중 역할 문제 (*p〈.05)에서 유의미한 변화를 보였다. 하지만 경제 문제, 가족 문제, 건강 문제에서는 유의미한 차이를 보이지 않았다. 뇌파에서는 육체적 스트레스와 자기 피드백 능력 베타1 (*p〈.05)에서 유의미한 차이를 보였다. 하지만 정신적 스트레스와 자기 피드백 능력에서 알파와 베타 2는 유의미한 차이를 보이지 않았다. 이상의 결과는 싱잉볼 자애 명상 프로그램이 생활스트레스와 뇌파에 개선 효과가 있는 것으로, 싱잉볼 자애 명상 프로그램을 객관적 근거인 뇌파로 탐색했다는 것에 그 의의를 들 수 있다. This study analyzes the effects of a singing bowl loving-kindness meditation program on life stress and EEG of adults. Two male and five female adult subjects between the ages of 40 and 64 years voluntarily participated in the study. The intervention period was 60 minutes per day for 7 days, from August 7 to August 13, 2022. Each session comprised a 20-minute introduction of self-reflection, a 30-minute main period of singing bowl loving-kindness meditation, and 10 minutes of final sharing. General characteristics and life stress were evaluated through a structured questionnaire, and EEG was measured for each individual. The collected data were verified through frequency analysis and Wilcoxon signed rank test to identify demographic and sociological characteristics using the SPSS/WIN 25.0 program. We observed that considering the sub-variables of life stress, the singing bowl loving-kindness meditation program imparts a significant change in role problem (*p<.05). However, no significant differences were obtained in the factors of economic problems, family problems, and health problems. EEG showed a significant difference in physical stress and self-feedback ability beta1 (*p<.05), but no significant differences between alpha and beta 2 in mental stress and self-feedback ability. Taken together, our results indicate that the singing bowl loving-kindness meditation program improves life stress and brain waves. We believe that exploring the singing bowl loving-kindness meditation program with objective evidence and EEG is meaningful with positive consequences.

Diagnostic Usefulness of IFN-Gamma Releasing Assays Compared With Conventional Tests in Patients With Disseminated Tuberculosis

Yu, Shi Nae,Jung, Jiwon,Kim, Yong-Kyun,Lee, Ju Young,Kim, Sun-Mi,Park, Su Jin,Lee, Sang-Oh,Choi, Sang-Ho,Kim, Yang Soo,Woo, Jun Hee,Kim, Sung-Han Williams & Wilkins Co 2015 Medicine Vol.94 No.28

<P><B>Abstract</B></P><P>IFN-gamma releasing assays (IGRAs) such as T-SPOT.<I>TB</I> assay and QuantiFERON-TB In-Tube (QFT-GIT) have yielded promising results for the diagnosis of tuberculosis (TB). However, little is known about the usefulness of these assays for diagnosing disseminated TB. We therefore compared their usefulness with traditional tests in patients with disseminated TB. All adult patients with suspected disseminated TB were prospectively enrolled at a tertiary hospital in an intermediate TB-burden country during a 6-year period. Disseminated TB was defined as involvement of the bone marrow or ≥2 noncontiguous organs, or presence of miliary lung lesions. A total of 101 patients with confirmed and probable disseminated TB were finally analyzed. Of these 101 patients, 52 (52%) had miliary TB and the remaining 49 (48%) had nonmiliary disseminated TB. In addition, 63 (62%) had no underlying disease. Chronic granuloma with/without necrosis, acid-fast bacillus staining, <I>Mycobacterium tuberculosis</I> PCR, and culture for <I>M tuberculosis</I> were positive in 77% (41/53), 43% (43/101), 70% (67/96), and 72% (73/101), of the patients, respectively. The T-SPOT.<I>TB</I> assay was positive in 90% (91/101) of them. The sensitivity of the T-SPOT.<I>TB</I> assay in patients with miliary TB (90%) was similar to that in patients with nonmiliary TB (90%) (<I>P</I> > 0.99). In a subgroup analysis of the 58 patients in whom both QFT-GIT and the T-SPOT.<I>TB</I> results were available, the sensitivity of QFT-GIT (67%) was lower than that of T-SPOT.<I>TB</I> (95%) (<I>P</I> < 0.001).</P><P>In conclusion, T-SPOT.TB assay may be a helpful adjunct test for disseminated TB.</P>

Reliability and Validity of the Comprehensive Limb and Oral Apraxia Test: Standardization and Clinical Application in Korean Patients With Stroke

Sung-Bom Pyun,Yu Mi Hwang,Soo Yung Jo,Ji-Wan Ha 대한재활의학회 2019 Annals of Rehabilitation Medicine Vol.43 No.5

Objective To develop and standardize the Limb and Oral Apraxia Test (LOAT) for Korean patients and investigate its reliability, validity, and clinical usefulness for patients with stroke. Methods We developed the LOAT according to a cognitive neuropsychological model of limb and oral praxis. The test included meaningless, intransitive, transitive, and oral praxis composed of 72 items (56 items on limb praxis and 16 items on oral praxis; maximum score 216). We standardized the LOAT in a nationwide sample of 324 healthy adults. Intra-rater and inter-rater reliability and concurrent validity tests were performed in patients with stroke. We prospectively applied the LOAT in 80 patients and analyzed the incidence of apraxia. We also compared the clinical characteristics between the apraxia and non-apraxia groups. Results The internal consistency was high (Cronbach’s alpha=0.952). The inter-rater and intra-rater reliability and concurrent validity were also high (r=0.924–0.992, 0.961–0.999, and 0.830, respectively; p<0.001). The mean total, limb, and oral scores were not significantly different according to age and education (p>0.05). Among the 80 patients with stroke, 19 (23.8%) had limb apraxia and 21 (26.3%) had oral apraxia. Left hemispheric lesions and aphasia were significantly more frequently observed in the limb/oral apraxia group than in the non-apraxia group (p<0.001). Conclusion The LOAT is a newly developed comprehensive test for limb and oral apraxia for Korean patients with stroke. It has high internal consistency, reliability, and validity and is a useful apraxia test for patients with stroke.