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      • KCI등재

        Genes of Wild Rice (Oryza grandiglumis) Induced by Wounding and Yeast Extract

        Shin,,Sang-Hyun,Im,,Hyun-Hee,Lee,,Jai-Heon,Kim,,Doh-Hoon,Chung,,Won-Bok,Kang,,Kyung-Ho,Cho,,Sung-Ki,Shin,,Jeong-Sheop,Chung,,Young-Soo Korean Society of Life Science 2004 생명과학회지 Vol.14 No.4

        야생벼의 일종인 Oryza grandiglumis (CCDD, 2n=48)는 도열병, 잎집무늬마름병, 흰빛잎마름병, 그리고 벼멸구와 같은 병충해에 저항성을 가지는 것으로 알려져 있다. 이와 같은 곰팡이와 해충에 반응하여 차별 발현하는 유전자를 클로닝 하기 위하여 상처처리와 yeast extract를 Oryza grandiglumis에 0시간과 24시간 각각 처리하였다. 유전자의 클로닝을 위하여 희귀 발현유전자의 클로닝에 효율적인 것으로 알려진 Suppression Subtractive Hybridization (SSH) 방법이 처리 후 24시간 된 식물을 재료로 사용되었다. 그 결과, 776개의 cDNA clones이 확보되었으며, 유전자 발현의 진위여부를 빠르게 스크린하기 위하여 colony array가 수행되었다. 115개의 colony가 positive로 판명되었고, 이들의 평균 insert size는 400 bp에서 700 bp에 이르렀고, 이들에 대한 염기서열 분석이 수행되었다. 염기서열 분석 결과, 68개 clone들이 알려진 기능의 유전자와 homology를 나타냈으며, 이중에서 16개 clone이 일차대사에 관련된 것과 유사성을, 5개가 plant retrotransposon과 유사성을, 5개가 식물 방어기작 관련 metallothionein-like gene과 염기서열 유사성을 보였다. 이외에 다양한 유전자들이 아미노산 합성관련, membrane transport, signal transduction등에 관여하는 유전자들과 상동성을 나타내었다. 이들 유전자중에서 4 개의 클론(ogwfi-161, ogwfi-646, ogwfi-663, ogwfi-695)들이 선발되었고 이들에 대한 Northern 분석이 수행되었다. Northern 분석 결과 ogwfi-161, ogwfi-646, ogwfi-663, ogwfi-695는 wounding과 yeast extract처리 에 의한 차별 발현이 확인되었다. 이상의 결과를 종합하여 볼 때, SSH방법은 병충해등과 같은 조건에 의해 차별 발현되는 유전자들을 빠른 시간 내에 다량으로 발굴할 수 있는 매우 효율적인 방법이라고 생각된다. Oryza grandiglumis (CCDD, 2n=48), one of the wild rice species, has been known to possess fungal-,bacterial-, and insect-resistance against sheath blight, rice blast, bacterial leaf blight and brown plant hopper (Nilaparvata lugens). To rapidly isolate differentially expressed genes responding to fungal and wounding stress, wounding and yeast extract were treated to O. grandiglumis for 24 hrs. Suppression subtractive hybridization (SSH) method was used to obtain differentially expressed genes from yeast extract and wounding treated plants. Seven hundreds and seventy six clones were obtained by subcloning PCR product, and colony array and screening were carried out using radio-isotope labeled cDNA probes prepared from the wounding and yeast extract treated plants. One hundred and fifteen colonies were confirmed as true positive ones. Average insert size of the clones were ranged from 400 bp to 700 bp and all the inserts were sequenced. To decide the identity of those clones, sequences were analyzed by sequence homology via GenBank database. The homology search result showed that 68 clones were matched to the genes with known function; 16 were related to primary metabolism, 5 to plant retrotransposons, 5 to defense related metallothionein-like genes. In addition to that, others were matched to various genes with known function in amino acid synthesis and processing, membrane transport, and signal transduction, so on. In northern blot analysis, induced expressions of ogwfi-161, ogwfi-646, ogwfi-663, and ogwfi-695 by wounding and yeast extract treatments were confirmed. The result indicates that SSH method is very efficient for rapid screening of differentially expressed genes.

      • KCI등재

        원저 : 임상 ; 경기도 서남부 지역의 단일 기관으로 전원된 미숙아로 살펴 본신생아 집중 치료의 지역화 진료 체계 및 이송 체계

        신상훈 ( Sang Hoon Shin ), 이은희 ( Eun Hee Lee ), 신정희 ( Jeong Hee Shin ), 황미정 ( Mi Jung Hwang ), 최영옥 ( Young Ok Choi ), 서원희 ( Won Hee Seo ), 최병민 ( Byung Min Choi ), 김해중 ( Hai Joong Kim ), 홍영숙 ( Young Sook Hong ) 대한주산의학회 2013 大韓周産醫學會雜誌 Vol.24 No.4

        목적: 국내에서는 고위험 신생아를 위한 지역화 진료 체계가 완전히 확립되어 있지 않고 더욱이 안전한 신생아 이송 체계가 구축되어 있지 않은 상태이다. 본 저자들은 고려대학교 의료원 안산병원 신생아중환자실로 이송된 35주 미만의 미숙아를 대상으로 이송에 따른 문제점과 이들 미숙아들의 임상 경과 및 합병증 그리고 사망률을 원내 출생한 35주 미만의 미숙아들과 비교 분석하여, 지역 내 신생아 집중 치료의 지역화 진료 및 이송의 현실과 문제점을 파악함으로써 향후 우리 나라 실정에 맞는 적절한 국내 신생아 집중 치료의 지역화 진료 체계 및 이송 체계를 구축하는데 도움이 되고자 하였다. 방법: 2006년 1월부터 2013년 6월까지, 재태 주령 35주 미만이면서, 타 병원에서 출생 후 생후 3일 이내에 고대안산병원 신생아중환자실로 전원 된 원외 출생아(40명)와 대조군으로 같은 재태 주령과 같은 출생 체중의 원내 출생아(40명)를 대상으로 의무기록을 검토하여 후향적으로 연구를 하였다. 결과: 입원 당시, 저혈당과 호흡곤란 증후군의 발생 빈도는 원외 출생아군에서 원내 출생아군보다 유의하게 높았다. 계면활성제의 사용빈도와 기계환기기 사용빈도, 심근수축제 사용빈도도 원외 출생아군에서 유의하게 높았다. 전원 전 계면활성제를 사용한 환아는 없었으며, 원외 출생아군에서만 4명(10%)이 사망하였으며, 원내 출생아군에서 사망한 경우는 없었다. 신생아 이송팀들의 대부분은 간호사만으로 구성되었고, 의사가 포함된 이송팀은 적었다. 결론: Level I의 의료기관에서 출생한 고위험 신생아의 사망률과 이병률을 감소시키기 위해서는 신생아의 안정화와 안전한 이송을 위한 의료진에 대한 교육이 필요하다. 그리고 각 Level 에 따른 적절한 의료 인력과 장비 및 설비 그리고 신생아 집중 치료 시설의 인프라와 지역화 진료 체계의 구축을 위한 제도적 기준이 필요하겠다. Purpose: Not only regionalization of neonatal care for high risk newborn, but also safe neonatal transport system of newborn were not completely established in Korea. The aim of this study was to compare the clinical outcomes of preterm infants less than 35 week gestational age with regard to inborn and out born status, to understand the problems of regionalization of neonatal care and neonatal transport system and to provide the basis to solve the potential problems. Methods: This retrospective study included 40 out born and 40 inborn preterm infants less than 35 week gestational age admitted to the neonatal intensive care unit of Korea University Ansan Hospital during the period between January 2006 and June 2013. Results: Compared with those in the inborn group, the incidences of hypoglycemia and respiratory distress were significantly more frequent in the out born group at admission. The uses of surfactant, ventilator, and inotrope were significantly more frequent in the out born during hospitalization. Mortality occurred only in theoutborn group. Most of infants were transferred by a nurse alone, not a team with doctor. Conclusion: Transferred preterm infants may not be sufficiently stabilized before transport, according to the result of more frequent hypoglycemia and respiratory distress in the out born group. In order to reduce mortality and morbidity of transferred newborn from level I, the national policy about neonatal intensive care unit level guideline (manpower, equipment, and facility), regionalization of neonatal intensive care, and neonatal transport system are needed in Korea.

      • KCI등재

        원통 확장부를 갖는 구형 LNG 탱크의 동적 설계하중 산출식 개발

        신상훈(Sang-Hoon Shin), 고대은(Dae-Eun Ko) 한국산학기술학회 2017 한국산학기술학회논문지 Vol.18 No.5

        LNG 운반선용 구형 탱크의 제작을 위해서는 탱크의 크기에 따라 별도의 공장 설비가 필요하며 시설 투자의 한계로 인해 현실적으로 조선소에서 다양한 크기의 완전 구형 LNG 탱크를 제작하는 것은 매우 어려운 일이다. 다양한 용량의 LNG 탱크 제작을 위해서는 기존 구형 탱크의 중앙부에 원통 형태의 확장부를 삽입하여 적재 용량을 키우는 방법이 효과적이다. 본 연구에서는 원통 확장부를 갖는 구형 LNG 탱크에 대하여 수평 가속도에 의한 동적 압력분포 산출식을 유도하였다. 본 논문에서 유도한 압력 분포 산출식을 이용함으로써 원통 확장부를 갖는 구형 탱크를 화물창 형식으로 하는 LNG 운반선의 구조설계 시 구조 안전성 평가를 위한 동적 설계하중을 간편하게 구하여 해석에 적용할 수 있다. 또한, 이미 개발되어 있는 원통 확장부를 갖는 구형 LNG 탱크의 정적 하중에 대한 설계하중 산출식과 결합함으로써 정적 및 동적하중을 모두 고려한 정도 높은 간이해석법의 개발이 가능하며, 이를 통해 초기 견적 시 많은 시간과 비용을 소요하는 유한요소 해석을 대신하여 짧은 시간에 정도 높은 물량 산출이 가능할 것으로 기대된다. The number of shops needed for the fabrication of a sphere type cargo tank for an LNG carrier is proportional to the size of the tank to be constructed. Due to the limitations of facility investment, it is difficult to fabricate various size tanks with a perfectly spherical shape in the (factory). An efficient method of increasing the capacity of the cargo tank is to extend the conventional sphere type LNG tank vertically by inserting a cylindrical shell structure. In this study, equations for the dynamic pressure distribution due to horizontal acceleration are derived for a sphere type LNG tank with central extension. The derived equations can be easily applied to the design and structural assessment of a sphere type LNG tank with central extension. Furthermore, the results of this study can be combined with the static design loads previously reported by Shin & Ko [9], in order to establish a simplified analysis method which enables a precise initial estimate to be obtained, thereby obviating the need for a time consuming finite element analysis.

      • SCOPUSSCIEKCI등재

        Recent Advancements of Treatment for Leptomeningeal Carcinomatosis

        Gwak,,Ho-Shin,Lee,,Sang,Hyun,Park,,Weon,Seo,Shin,,Sang,Hoon,Yoo,,Heon,Lee,,Seung,Hoon The Korean Neurosurgical Society 2015 Journal of Korean neurosurgical society Vol.46 No.4

        Treatment of Leptomeningeal carcinomatosis (LMC) from solid cancers has not advanced noticeably since the introduction of intra-cerebrospinal fluid (CSF) chemotherapy in the 1970's. The marginal survival benefit and difficulty of intrathecal chemotherapy injection has hindered its wide spread use. Even after the introduction of intraventricular chemotherapy with Ommaya reservoir, frequent development of CSF flow disturbance, manifested as increased intracranial pressure (ICP), made injected drug to be distributed unevenly and thus, the therapy became ineffective. Systemic chemotherapy for LMC has been limited as effective CSF concentration can hardly be achieved except high dose methotrexate (MTX) intravenous administration. However, the introduction of small molecular weight target inhibitors for primary cancer treatment has changed the old concept of 'blood-brain barrier' as the ultimate barrier to systemically administered drugs. Conventional oral administration achieves an effective concentration at the nanomolar level. Furthermore, many studies report that a combined treatment of target inhibitor and intra-CSF chemotherapy significantly prolongs patient survival. Ventriculolumbar perfusion (VLP) chemotherapy has sought to increase drug delivery to the subarachnoid CSF space even in patients with disturbed CSF flow. Recently authors performed phase 1 and 2 clinical trial of VLP chemotherapy with MTX, and 3/4th of patients with increased ICP got controlled ICP and the survival was prolonged. Further trials are required with newly available drugs for CSF chemotherapy. Additionally, new LMC biologic/pharmacodynamic markers for early diagnosis and monitoring of the treatment response are to be identified with the help of advanced molecular biology techniques.

      • SCIESCOPUSKCI등재

        A Novel Technique to Minimize Gain-Transient Time of WDM signals in EDFA

        Shin,,Seo-Yong,Kim,,Dae-Hoon,Kim,,Sung-Chul,Lee,,Sang-Hun,Song,,Sung-Ho Optical Society of Korea 2006 Journal of the optical society of Korea Vol.10 No.4

        We propose a new technique to minimize gain-transient time of wavelength-division-multiplexing (WDM) signals in erbium-doped fiber amplifiers (EDFA) in channel add/drop networks. We have dramatically reduced the gain-transient time to less than $3{\mu}sec$ by applying, for the first time to our knowledge, a disturbance observer with a proportional/integral/differential (PID) controller to the control of EDFA gain. The $3{\mu}sec$ gain-transient time is the fastest one ever reported and it is approximately less than 1.5% of $200{\mu}sec$ gain-transient time of commercially available EDFAs for WDM networks. We have demonstrated the superiority of the new technique by performing the simulation with a numerical modeling software package such as the $Optsim^{TM}$.

      • KCI등재

        Estimation of N Mineralization Potential and N Mineralization Rate of Organic Amendments in Upland Soil

        Shin,,Jae-Hoon,Lee,,Sang-Min,Lee,,Byun-Woo 한국토양비료학회 2015 한국토양비료학회지 Vol.48 No.6

        Management of renewable organic resources is important in attaining the sustainability of agricultural production. However, nutrient management with organic resources is more complex than fertilization with chemical fertilizer because the composition of the organic input or the environmental condition will influence organic matter decomposition and nutrient release. One of the most effective methods for estimating nutrient release from organic amendment is the use of N mineralization models. The present study aimed at parameterizing N mineralization models for a number of organic amendments being used as a nutrient source for crop production. Laboratory incubation experiment was conducted in aerobic condition. N mineralization was investigated for nineteen organic amendments in sandy soil and clay soil at $20^{\circ}C$, $25^{\circ}C$, and $30^{\circ}C$. N mineralization was facilitated at higher temperature condition. Negative correlation was observed between mineralized N and C:N ratio of organic amendments. N mineralization process was slower in clay soil than in sandy soil and this was mainly due to the delayed nitrification. The single and the double exponential models were used to estimate N mineralization of the organic amendments. N mineralization potential $N_p$ and mineralization rate k were estimated in different temperature and soil conditions. Estimated $N_p$ ranged from 28.8 to 228.1 and k from 0.0066 to 0.6932. The double exponential model showed better prediction of N mineralization compared with the single exponential model, particularly for organic amendments with high C:N ratio. It is expected that the model parameters estimated based on the incubation experiment could be used to design nutrient management planning in environment-friendly agriculture.

      • P083 : Biologic-resistant areas in Korean patients with moderate to severe psoriasis

        ( Sang-hyeon Won ), ( Bong Seok Shin ), ( Dae-lyong Ha ), ( Jin-hwa Son ), ( Jungsoo Lee ), ( Kihyuk Shin ), ( Hoon-soo Kim ), ( Hyun-chang Ko ), ( Moon-bum Kim ), ( Kyunghyung Seo ), ( Byung-soo Kim ) 대한피부과학회 2020 대한피부과학회 학술발표대회집 Vol.72 No.2

        Background: Psoriasis in certain body sites such as palmoplantar, nail, scalp and intertriginous areas has been known to be difficult to treat. However, to date, there have been no studies for biologic-resistant areas in patients with moderate to severe psoriasis in Korea. Objectives: To investigate the body location of treatment-resistant psoriasis in Korean patients treated with biologic agents. Methods: We included fifty biologic-resistant patients with moderate to severe psoriasis treated for > 6 months in Pusan National University Hospitals(Busan and Yangsan) and Chosun University Hospital. All patients' medical records, Psoriasis Area and Severity Index (PASI) score, the location of treatment-resistant psoriasis were investigated. Results: The mean age of the patients was 47.8±11 years, and 32 patients (64%) were male. The median PASI score (interquartile range) at treatment initiation was 15 (12.2-18.1), and current PASI score was 1.8 (1.4-2.8). The median PASI reduction from treatment initiation was 87.9% (81.3-92). The most common site of recalcitrant psoriasis was the anterior lower leg (56%) followed by the knee (48%), posterior lower leg (42%), scalp (38%), and back (34%). Conclusion: In real-world clinical practice, the most common sites of recalcitrant psoriasis in Korean patients treated with biologic agents are the anterior lower leg, knee, and posterior lower leg.

      • KCI등재

        Potential Methane Production on Anaerobic Co-digestion of Swine Manure and Food Waste

        Shin,,Joung-Du,Park,,Sang-Won,Kim,,Sang-Hyoun,Duangmanee,,Jack,Lee,,Po-Heng,Sung,,Shi-Hwu,Lee,,Bong-Hoon The Korean Society of Environmental Agriculture 2008 한국환경농학회지 Vol.27 No.2

        Anaerobic co-digestion of swine manure and food waste for biogas production was performed in serum bottles at various volatile solids(VS) contents and mixing ratios of two substrates(swine manure:food waste=$100:0{\sim}0:100$). Through kinetic mode of surface methodology, the methane production was fitted to a Gompertz equation. The ultimate methane production potential of swine manure alone was lower than that of food waste regardless of VS contents. However, it was appeared that maximum methane production potentials in 80 : 20 of the mixing rate at VS 3% was enhanced at 144.7%, compared to its only swine manure. The potential increased up to 815.71 ml/g VS fed as VS concentration and food composition increased up to 3.0% and 20%, respectively. The ultimate amount of methane produced had significantly a positive relationship with that of methane yield rate. Overall, it would be strongly recommended that feeding stocks use 20% of mixing ratio of food waste based on VS 3% contents when operating the anaerobic reactor on site at $35^{\circ}C$ if not have treatment of its anaerobic waste water.

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