붕어(Carassius auratus)에 있어서 benzocaine, clove oil 및 tricaine methanesulfonate(MS-222)의 마취에 따른 혈장 cortisol 농도의 변화

임현정, 정태성, 조성준, 허강준 충북대학교 동물의학연구소 2007 Journal of Biomedical and Translational Research Vol.8 No.1

본문참고

경주시 보호수 생육실태 연구

허상현,하재호 한국환경과학회 2004 한국환경과학회지 Vol.13 No.10

The purpose of this study is to survey and analyze the growth, management and surrounding environment of the big and old trees in Kyoungju-si or the cultural assets alive in our history, and thereby, provide for some data useful to their reasonable protection and use of their surrounding areas. As a result of surveying the growth conditions of the big and old trees, it was found that the height of new grass was 10.5cm on average, the activity scale of the wood was 7.2kΩ, the soil hardness was 16.7kg/㎠, the soil acidity was pH 4.8, and the soil moisture was 13.3%. Such findings suggest that the soil has been acidified by people's frequent passages, but that the other growth conditions are more or less normal. Hence, it is desirable to secure a sufficient space around the trees or reduce people's stamping pressure with some mechanisms. On the other hand, the visible conditions of the trees were found more or less normal, but many trees remained cut or barked (with some cavities), requiring an optimal treatment or measure. Lastly, as the population has decreased in the suburban traditional villages, the surrounding environment seems to be less vulnerable to people's frequent visits. Nevertheless, in consideration of the fact that there are only a few public space for the villagers, it is deemed necessary to rearrange or maintain some parts of the surrounding environment as public space for villagers or hikers.

PDGF와 LPS가 치주 인대 세포의 활성에 미치는 영향에 관한 연구

허정,김상철,임정현 대한치과교정학회 1998 대한치과교정학회지 Vol.28 No.1

PDGF와 LPS는 골 대사에 중요한 조절요소이다. 외인성 PDGF는 골 흡수를 자극하기도 하고, 또 새로운 골 형성을 자극하기도 한다. LPS는 골파괴 활성의 자극요인으로 알려져 있다. 본 연구의 목적은 골개조에 중요한 역할을 하는 치주 인대에 PDGF와 LPS의 상호 작용을 평가하기 위해서 시행되었다. 배양된 사람의 치주인대 세포에 PDGF와 LPS를 첨가 배양하여 각 농도와 시간에 따른 대조군에 대한 백분율로 환산된 세포 활성의 평균과 표준편차를 구하고 ANOVA를 시행하여 다음과 같은 결과를 얻었다. 1. PDGF를 단독으로 가한 경우 0.1ng/ml에서는 세포활성이 대조군과 차이가 없었으나 1mg/ml에서는 배양 2일째에, 10ng/ml에서는 배양 2,3일째에 유의한 세포 활성 증가를 보였다. 2. LPS 단독처리시 0.5㎍/ml 및 5㎍/ml 농도에서 배양 1일째, 2일째에는 대조군과 차이가 없었으나 배양 3일째에 유의한 활성 감소가 없었다. 3. 0.5㎍/ml 및 5㎍/ml 농도의 LPS에 1ng/ml 및 10ng/ml농도의 PDGF첨가시, 배양 3일째에 LPS단독 투여시보다 유의한 세포 활성 증가가 있었으며 특히 10ng/ml PDGF 첨가시에는 대조군보다 활성이 컸다. 이상의 연구결과로 골개조 과정에 관여하는 것으로 여겨지는 치주 인대세포에 대한 적절한 농도의 PDGF와 LPS의 복합투여는 PDGF 단독 투여 못지 않게 치주 인대세포의 활성을 증가시키는 것으로 사료된다. Platelet-derived growth factor(PDGF) and lipopolysaccharide(LPS) may be the important regualtors of bone metabolism. Exogenous PDGF is recognized to have a stimulating effect on bone resorption in organ culture, but to stimulate the formation of new bone ultimately. LPS is known to be a stimulating agent on the osteoclastic activity. The purpose of this study was to evaluate the effects and the interaction of PDGF and LPS on periodontal ligament(PDL) cells which have important roles in bone remodeling. Cultured human periodontal ligament cells were treated with various concentration of PDGF and/or LPS. The cellular viability was measured by Microtitration(MTT) assay according to the lapse time of culture. The obtained results were as follows: 1. The viability of PDL cells was not different from the control in 0.1ng/ml of PDGF, but was significantly increased to be over the level of control in 1ng/ml of PDGF at the second day of culture, and in 10ng/ml of PDGF at the second and the third day of culture. 2. The cellular viability was decreased in 0.5㎍/ml or 5㎍/ml of LPS at the third day of culture. 3. Incubation with both 1ng/ml or 10ng/ml of PDGF and 0.5㎍/ml or 5㎍/ml of LPS resulted in the increased cellular viability at the third day, which was greater than LPS only treated group. It was greater than even the control group in 10ng/ml of PDGF. From the above findings, we could summarize that the admixture of PDGF and LPS could not less increase the viability of the human periodontal ligament cells than PDGF only.

일반인의 환경문제 의식과 사회환경교육의 필요성에 관한 연구

허명,최돈형,박소현,성효현,박석순,남상준,이숙임 이화여자대학교 환경문제연구소 1998 이화환경연구 Vol.2 No.-

Today, the gobal community is confront serious environmental crisis derived from related problems energy and resources. To deal this crisis, people must take action in order to preserve current environment with ecological understanding of the relationship between human and environment. The principle of this thesis is to observe public endeavor toward environmental education program, environmental perception and environmental preservation behavior, and the influential factors to those conducts. First of all, we will look into the perception toward environmental deterioration, environmental perception, environmental preservation behavior which thought to be a ground solution to lesson the problems, and then the environmental education program. In accordance with those research, it will be presented the feasible directions of environmental education program for general public. This research consists of 10 topics. The topics are the interests of public about environmental problem, perceived pollution level, general knowledge about environment, perception related to environmental problem, opinions about the environmental education program, attitude towards environmental organization, suggestions for future environmental education program, and campaign for environmental preservation. Through the research, we have reached some analytical conclusions are followed: First, the level of public concerns about environmental problems is high regardless its age and sex, and the level has been growing compared to the past one. The result also shows that people in urban and industrial areas feel much more seriously pollution problems than those in rural and coastal areas. In addition, according to the survey result, household and industrial sewage are regarded as the primary causes for water pollution. Secondly, the results show general public's understanding on environment have been universalized and thus the level of common knowledge on global environment has been increased, as environmental problems have been raised by newspapers and mass medias and various movements have been accomplished by environmental protection organizations. Thirdly, although the necessity of environmental education was emphasized the purpose of environmental education was unclearly appeared. Furthermore, actual participation to the environmental education program and taking courses by joining environmental organizations were rarely shown. Although some cases have the experience of taking education courses, the negative reactions came out in the opinion of education content and methodologies. Fourthly, the analysis on curriculum of future environmental education show various opinions. The attentions have drawn on the dealings of waste industrial and chemical waste which will cause environmental problem artificially, as well as on global environment, or natural environment such as resources, land, water, atmosphere. The results show many people recognized administration extent such as environmental policy, law and ethics as environmental categories. This implies the curriculum of those areas have not been properly founded and further researches should be followed. From above results, environmental problems should not be regarded as simple problems and it should bring out new recognition for general public in everyday life. In addition, environmental education courses, with practical environmental preservation should be developed and programs and organization which will utilize those educational materials should be more activated.

초등학교 과학실에서 발생하는 화학폐기물에 대한 실태

김성규,허승현 한국환경과학회 2004 한국환경과학회지 Vol.13 No.3

The purpose of this study was to examine teachers' awareness of chemical waste produced in elementary school laboratory experimentation and how this awareness relates to collection and disposal of chemical waste. More specifically, the study looked at the correlation between the handling of chemical waste and factors such as years of teachers' educational career, class size and amount of waste produced. The target population were 250 elementary school teachers in Gyeongnam area and 237 subjects were responded. Among the 237 responses, 37 cases that did not complete the questionnaire were eliminated. Therefore, 200 responses were analyzed in this study. The survey questionnaire consisted of 15 questions. The categories of the questionnaire were their skills of management and treatment of the chemical waste. The data collected were analyzed by SPSS 10.0, and the relations among variables such as class sizes and years of teaching experience were also analyzed by x²-test. The results in this study were as follows: First, there were no significant differences between the years of teaching and class sizes in the training experience of chemical waste disposal. Second, there was a significant difference between the science laboratory size and class sizes in the laboratory actual condition. In addition, in the relations between the number of times of experimentation and the years of teaching experience, there was a significant difference. Third, in terms of the discharge amount of chemical waste, there was a significant difference between the years of teaching and class sizes. Fourth, in the simple chemistry waste disposal process in the science laboratory, there also was a significant difference between the kinds of experimental equipments that used in the experimentation and the years of teaching. Based on this study, it was found that great amount of the chemical wastes produced in the science laboratory dumped into the drain and the treatment process of chemical waste was also inattentive. Even the importance of environmental education is emphasized in the elementary education, the basic problems occurred in the science laboratory is disregarded. Therefore, not only students but teachers have to pay attention to the disposal process of chemical waste in the laboratory in order to prevent environment pollution. Furthermore, the efforts of preventing environment pollution are needed such as opening the teacher training course about environment education, minimal use of chemicals, treatment of chemical waste, and so forth.

위궤양에 대한 Teprenone(Selbex^�)의 임상효과

정현용,김병호,성자원,이기천,허승식,이종선,이헌영,김영건 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

The study objective was to assess the ulcer healing effects of Teprenone, stimulator of mucus production, given in dose of 50mg tid. We reviewed 25 cases of gastric ulcer proven endoscopically and treated by Teprenone. The results were follows; 1) The healing rate of gastric ulcer was 72% after 8 weeks treatment. 2) Subjective symptoms such as epigastric pain, belching, nausea, anorexia and vomiting were subsided at 86%. 3) There were not developed any symptoms, signs and laboratory abnormality (CBC, liver funtion test, urinalysis) suggesting side effect of drugs. In conclusion, Teprenone was considered an effective and safe drug in the treatment of gastric ulcer diseases.

상부소환관협착에 대한 Savary-Gilliard Dilatation의 치료효과와 안전성에 관한 관찰

정현용,육은주,임의혁,김병호,성자원,이기천,허승식,이헌영,김영건 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

For the patient with stenosis of upper digestive tract caused by either benign or malignant process, esophageal dilatation is an important therapeutic modality. We reviewed retrospectively 39 cases treated by Savary-Gilliard dilatation for upper digestive tract stenosis, and results were follows : (1) Dysphagia was improved in 95% of the patients. (2) Eight patients(20%) sufferd perforation, five of them were managed with conservative medical measures, others were managed operatively. (3) In the cases with benign stenosis dysphagia was not noticed for 8.8 months, but with malignant stenosis dysphagia was reappeared after 2.8 months despite concomitant chemoradiotherapy. In conclusion, Savary-Gilliard dilatation was an effective measure for symptome due to upper digestive tract stenosis, but more careful attention for perforation should be necessitated. Also another therapeutic modality for treatment of malignant stenosis was inevitable.

백서의 하악골 전방 재위치 시 하악과두 조직에서의 nitric oxide synthases 발현 양상

김현숙,김호영,허성수,강경화,김상철 대한치과교정학회 2010 대한치과교정학회지 Vol.40 No.4

Objective: The aim of this study was to identify the expression of nitric oxide synthases (NOS) in the mandibular condyle during mandible advancement by functional appliance and to correlate it with the histologic changes and bone remodeling. Methods: Twenty-four female, 35-day-old Sprague-Dawley rats were randomly divided into 3 experimental groups. In all experimental groups, the mandibles of the rats were kept in a continuous forward position with a fixed bite jumping appliance. The rats were sacrificed on the 3rd, 14th, and 30th days of experiment. More than 2 rats in each group were used for staining. Results: There were no remarkable histologic changes and NOS expression differences in the control group. The most prominent histologic changes occurred in the 14th day experimental group. NOS decreased in the 30th day experimental group. There was increased expression of NOS₂ and NOS₃ in all experimental groups, comparative to the control group. In all the experimental groups and control group, the expression of NOS₂ was greater than that of NOS₃. Conclusions: It is postulated that NOS₂ and NOS₃ in the mandibular condyle might play an important role in bone remodelling of the mandibular condyle. 성장기 아동의 하악과두 조직에서의 골개조 과정은 연골세포, 조골세포, 파골세포가 연관되며 nitric oxide synthase (NOS)가 중요한 매개자의 하나로 작용할 것으로 생각되나 기능성 악정형술을 이용해 하악골을 전방유도시켰을 때 하악과두에서의 골개조와 NOS 의 발현에 대해서는 아직 자세히 연구되어 있지 않다. 본 연구는 하악골을 기능적으로 전방위치시켰을 때 하악과두 조직의 조직학적 변화양상에 대해 살펴보고 NOS 의 발현 정도를 조사하여 상관관계를 알아보고자 하였다 . 35일령의 24마리의 Sprague-Dawley 백서를 3군으로 나누었고 각 군에 있어서 5 마리는 실험군으로 3마리는 대조군으로 나누었다. 실험군은 하악골 전방유도장치를 24시간 장착한 후 3, 14, 30 일에 희생하였으며 시상면에 평행하게 절단하였고 NOS₂와 NOS₃의 조직면역염색을 통해 다음과 같은 결과를 얻었다. 대조군 간에는 조직학적인 변화와 NOS 의 발현 번화가 뚜렷이 관잘되지는 않았으며 실험기간에 따른 조직학적 변화는 실험 14일 군에서 가장 많은 신생골 형성과 조골세포 활성을 보였으며, 실험 30 일 군에서는 감소하는 양상으로 나타났다. 또한 모든 실험군에서 대조군에 비해 NOS₂와 NOS₃의 발현이 많이 나타났으며 실험기간에 따른 비교 시 NOS₂와 NOS₃의 발현은 실험 14 일 군에서 가장 많이 나타났으며 실힘 30 일 군에서는 감소하는 양상으로 나타났다. 전체 실험군과 대조군에서 NOS₂의 발현은 NOS₃의 발현보다 많이 나타났고 NOS₂는 실험군 과두의 비대연골, 연골하골에서 주로 발현되었다. NOS₃는 실험군 과두의 골수와 모세혈관에서 주로 발현되었다. 따라서 하악과두 내의 NOS₂와 발현조절이 하악과두 조직의 골개조에 관여함을 시사한다.

급성 및 만성 간질환에서 C형 간염바이러스 항체(anti-HCV)양성률

김삼용,성자원,김병호,이기천,허승식,길준영,정현용,이헌영,김영건 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

The prevalence of antibody against hepatitis C virus (anti-HCV) was investigated in 331 patients with various liver diseases from April 1992 to September 1992. The presence of anti-HCV was detected by Lucky HCD EIA test in 331 cases and by (Ortho HCV Antibody ELISA test 148 patients) The results are summarized as follows: 1. Overall, antibody to HCV(anti-HCV) was positive in 37(11%) of 331 patients with various liver diseases. 2. 16(11%) of 144 patients with chronic hepatitis, 2(5%) of 37 patients with alcoholic liver disease, 17(22%) of 76 patients with liver cirrhosis and 2(5%) of 38 HBsAg carriers were positive for anti-HCV.None of 12 patients with acute hepatitis, 11 patients with drug induced hepatitis and 13 patients with hepatocellular carcinoma was anti-HCV positive. 3. HBsAg positivity in patients who had anti-HCV was 24%(9/37). 4. A positive correlation was found between Lucky HCD and Ortho HCV test. Of 128 Lucky HCD negative cases, 124 cases were negative for Ortho HCV ELISA and 4 cases were positive for Ortho HCV ELISA Of 20 Lucky HCD positive cases, 11 cases were positive for Ortho HCV ELISA and 9 cases were negative for Ortho HCV ELISA. These results suggest that hepatitis C virus has an important etiologic role in HBsAg negative chronic hepatitis and liver cirrhosis in Korea. The diagnostic value of Lucky HCD EIA test may be slightly better or equivalent to Ortho HCV ELISA test in the diagnosis of Hepatitis C virus infection.

내시경으로 확인된 상부소화관 용종의 임상적 고찰

김영건,김병호,성자원,이기천,허승식,이종선,정현용,이헌영 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

From January 1987 to October 1991, we performed 14,333 cases of upper gastrointestinal endoscopy and diagnosed 527 cases with U.G.I polyp. We assesed these 527 cases and obtained following results: 1) The overall incidence of U.G.I. polyps was 3.6%, and there was no sexual difference. The peak incidence was in 6th decades(32.4%). 2) The U.G.I. polyps were located chiefly at stomach(415 cases, 78.7%), among which antrum occupied antrum 43.5%, body 24.0% and funds 5.1%. And the others were esophagus(6.5%) and duodenum(12.3%). 3) The size of U.G.I. polyp was below 1cm in 72.3%, from 1cm to 1.9cm in 20.7% and above 2cm in 7.0%. According to Yamada' classification, type Ⅰ, Ⅱ, Ⅲ and Ⅳ was 31.9%, 51.3%, 13.7% and 3.0%. 4) Histological nature of U.I.G. polyp were hyperplastic polyp(85.8%), adenomatous polyps(9.3%), carcinomatous polyps(3.3%) and etc. The size of neoplastic polyp such as adenomatous polyps(mean 1.0±0.7cm) and carcinomatous polyps(niean 2.0±1.8) were larger man hyperplastic polyp(mean 0.6±0.4) (p<0.05) 5) The gastric polyps were associated with peptic ulcer(7.5%), gastric cancer(3.2%), other malignancy(3.5%), hepatobiliary disease(5.5%), post subtotal gastrectomy(5.5%). And others(75%) werenot associated with specific disease.