녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

성인에서 발생한 발진성 가성 혈관종증

서상희 ( Sang Hee Seo ),장호선 ( Ho Sun Jang ),목혜수 ( Hye Soo Mok ),김성준 ( Sung Jun Kim ),김병수 ( Byung Soo Kim ),김문범 ( Moon Bum Kim ),오창근 ( Chang Keun Oh ),권경술 ( Kyung Sool Kwon ) 대한피부과학회 2007 대한피부과학회지 Vol.45 No.8

Background: Eruptive pseudoangiomatosis (EPA) is a rare, benign, spontaneously regressing childhood exanthem. It is characterized by the sudden onset of several bright red angioma-like papules surrounded by pale halos with a distinct histopathology from true angiomas. Objective: This study was performed to evaluate the clinical and histopathologic characteristics of EPA occuring in adults. Methods: Ten adult patients who visited Pusan National University Hospital and Mok Hye-SooㆍJang Ho-Sun Dermatology Clinic from March 2005 to September 2006 were evaluated. We prospectively evaluated the sex, age, onset season, past medical history including immunosuppressive abnormalities, systemic disorders and other diseases including allergies. We also investigated the relations of mosquito biting, patients` occupations and outdoor activities to occurrence of EPA. In addition, simultaneous occurrence in family members, the clinical, histopathologic, laboratory findings, disease courses and responses to treatment were evaluated. Based on medical records, photographs and pathologic slides, we retrospectively diagnosed another 20 EPA patients suspected as insect bite from October 2003 to March 2005. The same questions were inquired as for the 10 patients who prospectively underwent evaluation. Results: In the study, female predominance (76.7%) was observed and the average age of onset was 54.2 years. Interestingly, there was no child patient during the period of study. Multiple, 2∼5 mm sized, red angiomatous papules surrounded by pale halos occurred on exposed areas such as the arms (86.7%), legs (50%), and face (46.7%), although it could also occur to a non-exposed area. EPA occuring in adults usually appeared in summer (80%). The mean disease duration was 3.4 weeks. Although EPA spontaneously regressed, it had the potential of recurrence (46.7%). Histopathologic findings showed dilated dermal blood vessels without the evidence of increase in numbers, and perivascular lymphocytes infiltration. Inside the lumen of dermal blood vessels, plump endothelial cells were found. Conclusion: EPA occuring in adults usually happened to exposed sites in summer, so it can be misdiagnosed as insect bite. We suggest that dermatologists should be concerned about EPA in adults and conduct further investigation to have a better understanding of the disease. (Korean J Dermatol 2007;45(8):797∼803)

사진을 활용한 시 창작 교육의 가능성

장화심,유성호 한국교원대학교교육연구원 2006 敎員敎育 Vol.22 No.3

창작은 더 이상 전문가들만의 것이 아니라 학습 과정에 있는 창작 주체 즉 학습 작가가 수행하는 문학적 글쓰기의 과정과 결과를 포함해야 하며, 따라서 '창작 교육'은 전문적인 작가를 배출하는 것을 목적으로 하는 것이 아니라 학습자의 문학적인 창작의 욕구를 자연스럽게 분출하여 생산 하도록 해야 한다. 따라서 시 창작 교육에서도 쓰는 경험을 제공하고 표현 과정을 중시해야 한다. 그래서 시 창작 교육은 단순한 감정 표현만이 아니라 인식의 차원에서 이루어지는 세상 읽기 교육이 되어야 하며, 경험이 부족한 학생들에게 인식의 폭을 넓히고 세상 읽기가 가능하도록 해야 한다. 이때 다양한 매체를 활용하면 효과가 배가된다. 여기서 사진의 매체적 특성은 매우 시사적인 가능성을 준다고 할 수 있다. 요컨대 사진을 활용한 교수 학습 활동은 사진 텍스트 읽기와 시 창작을 결합시키는 일이 될 것이다. 사진 안에는 내 삶을 돌아보게 만드는 남의 삶이 담겨 있다. 사진을 보고 학습자 자신의 사고를 글로 표현하는 과정에서 삶과 세계를 이해하고 주위 대상에 관심을 갖게 되며, 대상의 의미 해석을 통해 자신의 세계관을 확대하고 바람직한 세계관을 형성할 수 있다. 즉 간접 체험을 통해 인생과 삶에 대한 폭넓은 이해를 쌓고 나아가 자신의 삶을 되돌아보고 미래의 행동 지침으로 삼게 만들 수 있는 것이다. 또 사진을 보고 시를 창작하는 활동은 '읽기'와 '쓰기', '이해'와 '표현'이라는 상반된 것처럼 생각되었던 국어 교육의 내용을 결합하는 통합적 교육 효과를 얻을 수 있을 것이다. 또 인쇄 매체에 한정된 기존의 '읽기' 개념을 더 확장하여 문화 읽기로 나아가며, 작지만 매체의 변화를 고려한 국어 수업이 될 수 있을 것이다. Literature education needs to contribute to the fostering of a self-directed and active person. The crux of developing a self-directed and active ego-formation can be reached by encouraging students to engage in creative writing. However, literature education has been focused on how the literary works should be understood and appreciated. Literature education is introduced in the 7th national curriculum as an attempt to realize the ultimate goal of literature education, but has not been carried out well in the classrooms of individual schools. The part of' Literature reception and creative writing' includes' creative reconstruction of a literary work' and' creative writing of literature', in which the art of creative writing through' imitation' and' revision' is presented in the content organizations in the area of literature in the high school curriculum. In order to overcome this limitation of creative writing without any teaching method, a variety of attempts have been carried out. Among them, there are a great many of creative writing teaching methods through the formal elements, such as metaphor and rhythm, and techniques of expression. But, it is writing materials, not poetic expression that counts in the creative writing of poetry. Researches on mind-map, brainstorming, and free association as methods to make writing materials rich have been conducted, but it's difficult to draw pedagogical implications from the result of these researches for teaching creative writing to students with limited experience and knowledge. The purpose of this study is to present and propose a method of literature education that can engage the students in creative writing of poetry along with literature appreciation. However, this study shows a few limitations: not dealing with all the processes and methods for students' idea-making in a creative writing class, not analyzing the quantitative data showing the substantial improvement level of the students, and restricting them within the qualitative analysis of concrete situations of teaching of idea-making through the interaction between the teacher and the students.

스포츠센터 관계마케팅 실행요인이 관계품질, 구매행동에 미치는 영향

장호성,석용근 龍仁大學校 體育科學硏究所 2008 體育科學硏究論叢 Vol.17 No.1

The meaning of this research work lies in providing useful basic resources which can be utilized in the strategic respect of internal marketing by investigating how sports center customer in relation to relationship marketing implement factors on relationship quality and purchasing behavior, and by identifying how their service orientation affect business performance taking service quality as parameter. Customer of sports centers in Chungnam region were selected as the subjects of this survey and 248 participants were sampled by Convenience Sampling Method. Resulting data were processed by Frequency Analysis, Exploratory Analysis, Correlation Analysis, Regression Analysis using SPSS 11.0 program. The results from these research methods and analyses revealed the followings: First, results from regression analysis of performance factors of relationship marketing and relations quality revealed that customer-relations orientation, responsiveness and incentive had significant influences on reliability, and that customer-relations orientation and responsiveness, on involvement. Second, results from regression analysis of performance factors of relationship marketing and purchase behavior exhibited that incentive had a significant influence on repurchase intention, and that responsiveness and incentive, on positive word of mouth. Third, results from regression analysis of relationship quality and purchase behavior revealed that there were significant influences of all variables including reliability and involvement on repurchase intention and positive word of mouth.

불소함유 접착 테이프의 상아질 지각과민증 치료효과

장향길,이난영,이상호 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.3

본 임상연구는 상아질지각과민증을 치료하기 위한 여러 가지 방법 중 효과적인 방법의 하나로 알려져 있는 불소(NaF)를 이용하여 불소테이프(SCMC-T-5)를 개발, 제조하고, 이를 이용하여 상아질 지각과민 치료 효과를 기존의 불소바니쉬(Cavity Shield™)와 비교, 평가하였다. 상아질 지각과민증이 있는 건강한 20대 남녀 22명(88개 치아)이 본 임상연구에 참가하였으며, 각각 11명씩 불소테이프를 부착시킨 실험군과 불소바니쉬를 도포한 대조군으로 분류하였다. 불소 제제는 제조사의 지시에 따라 적용되었으며, 불소 제제 도포 전, 도포 3일후, 1주일 후, 4주일 후 대상치아에 압축공기와 얼음막대를 이용하여 자극을 가한 뒤 visual analog scale(VAS)을 이용하여 통증 정도를 표시하도록 하였다. 실험군에서 VAS scores는 초기에 38.636(air), 62.273(ice), 3일 후 30.273(air), 49.545(ice), 1주일 후 28.182(air), 40.000(ice), 4주일 후 26.364(air), 37.727(ice)이었으며, 초기 VAS socres에 비해 모든 VAS scores는 통계학적으로 유의성 있게 감소되었다. 대조군에서 VAS scores는 초기에 42.274(air), 65.909(ice), 3일 후 34.091(air), 55.909(ice), 1주일 후 28.636(air), 40.909(ice), 4주일 후 27.727(air), 31.364(ice)이었으며, 3일 후에 시행된 압축공기에 의한 자극검사를 제외한 모든 VAS scores는 초기 VAS socres와 비교했을 때 통계학적으로 유의성 있게 감소되었다. 본 임상연구에 사용된 불소테이프와 불소바니쉬는 상아질 지각과민증을 효과적으로 감소시켰고, 두 약제간의 치료효과의 차이는 없었다. In this clinical research, the fluoride tape(SCMC-T-5) using fluoride(NaF) was developed and manufactured and the treatment effect of the fluoride tape in dentin hypersensitivity patients was evaluated and compared with the effect of existing fluoride varnish(CavityShield™). Twenty two healthy adult patients(88 teeth) having dentin hypersensitivity participated in this clinical research and they were divided into two groups. The fluoride product was applied according to the manufacturer's instruction and the level of pain in the tooth after giving irritation using compressed air and ice stick was measured just after the application, after 3 days, after a week and after 4 weeks each using visual analog scale(VAS). In the experimental group, compared with the early VAS scores, all other VAS scores showed the significant decreases statistically. In the control group, all VAS scores except the VAS score of 34.091(air) measured 3 days after(using the irritation examination by the compressed air) showed the significant decreases statistically when compared with the early VAS scores. The fluoride tape and fluoride varnish used in this clinical research were able to treat the dentin hypersensitivity effectively.

폐기물 성상과 매립방식에 따른 침출수 특성

성낙창,김은호,문추연,김정권,장성호,김수생,박출재 동아대학교 환경문제연구소 1996 硏究報告 Vol.19 No.1

In this study, we can be obtained the following conclusions about the characteristics of leachate with waste compositions and landfill methods. pH shows a tendency to increase in E. landfill, because NH₃ is created by anaerobic degradation and dissolved in leachate. The concentrations of BOD and COD become different, In case of E. landfill, food of combustible is H. & S. landfill and then SS is high in concentration. According to passed time, T-N is high in concentration, but T-P shows a similar tendency. Heavy metals of leachate is lower than threshold concentrations. If leachate is treated biologically, microbes are not inibitory.

Clozapine 투여후 발생한 급성 간염

장순주,이호택,백주희,이상연 대한신경정신의학회 1999 신경정신의학 Vol.38 No.1

Clozapine 투여 후에 급성 간염이 발생했던 두 명의 환자에 대한 증례로, 두 명 모두 이전에 약물에 이한 간염이 발생한 과거력이 있었던 환자로서 무증사의 급성 간염이 발생한 후에 보존적 치료 후에 호전 되었다. 간염이 발생한 후에 간효소수치에 대해 정기적으로 검사를 하면서, 사용하고 있던 clozapine의 용량을 낮춘 후 간장제를 투여함으로써 간효소수치는 정상화되었다. Clozapine과 비교하면 clozapine은 간세포의 cytochrome P450에 영향을 미치는 것으로 생각되고 있고 chlorpromazine은 간내 담즙저류에 영향을 미치는 것으로 생각된다. 과거 약물에 의한 간염을 경험한 환자는 clozapine에 의해 유도된 감염에 보다 취약성을 보일 가능성이 있으며, 이런 관점에서 이전의 간 기능 이상을 경험했거나 물질 사용 장애 등의 병발이 있을 경우 정기적인 간기능 평가가 임상적으로 중요할 것으로 판단된다. The authors report two cases of clzapine-induced acute hepatitis. Two patients developed asymptomatic hepatitis and got better with conservative care. We decreased the dosage of clozapine and added hepatic protections, resulting in normalized laboratory findings. The authors also reviewed side effects of clozapine in this report. We reviewed the suggested mechanism of either clozapine or chlorpromazine-induced hepatitis. clozapine influences the liver cell via cytochrome P 450 and chlorpromazine does so via mild cholestasis. There may be a possibility that a patient who has experienced drug-induced hepatitis is vulnerable to clozapine-induced acute hepatitis. In this respect, those who have experienced drug-induced hepatitis must be observed more closely.

제주지역 관광수입 통계에 관한 조사연구 : 여행경비기준산출을 중심으로 The Cheju Island Case

장성수,송재호 한국관광정책학회 2000 觀光政策學硏究 Vol.6 No.1

To make the efficient tourism policy and manage the tourism business is it necessary that the tourism statistics should precisely be drawn up, and the tourist and tourism receipts, the fundamental items of tourism statistics, be estimated precisely and periodically. This survey, which is based on the above recognition calculates the criteria of the per capita tourism expenditures for the purpose of precisely measuring the tourism receipts in cheju island. In addition, the criteria of tourism expenditure and tourism receipts officially published by the Cheju Provincial Government and Cheju Travel Association is compared and analysed. According to the integration of the survey analysis executed four times, per capita tourism expenditure except the round air fare is surveyed 261,175won for the group tourist, 137,544won for the school excursion, 580,204won for the honeymoon, 293,418won for the family tourist, 235,007won for the individual tourist, 2,165,561won for Japanese, 473,007won for Chinese, and 440,683won for the English-Speaking tourist. This results indicates that all travel patterns except the school excursion is higher than the official criteria of Cheju Province Office. Estimating the tourism receipts of Cheju island in 1999 based on the criteria of tourism expenditures suggested by this survey is estimated 1,745,200,000,000 won including the one-way air fare, which is more than 700,000,000,000 won compared to 1,295,000,000,000 won which is the criteria of Cheju Provincial Government. The contents of this study are as follows : I. Purpose of this study, Ⅱ. Conceptual system of the tourism receipts and expenditures, Ⅲ. Analysis of the tourism statistics in Cheju Island, Ⅳ. Survey Design : modelling and sampling, Ⅴ. Results and Implications, Ⅵ. Concluding remarks.

수용성 키틴이 백서 창상치유에 미치는 영향

강장석,조용남,정상훈,유 결,임진수,권 호,조용우,고석원 大韓成形外科學會 2001 Archives of Plastic Surgery Vol.28 No.1

SAN을 이용한 큐잉모델 해결방법

장경수,신호진,이상호,김병기,신동렬 成均館大學校 科學技術硏究所 2000 論文集 Vol.51 No.2

Queueing analysis, in general., has the intrinsic problem of large state space dimension and complex computation. In fact, the petri net is a graphical and mathematical model. It has been used for the modeling of various applications, in particular, manufacturing systems. It can deal with parallelism, concurrence, deadlock avoidance, and asynchronism. Currently it has been applied to the performance of computer networks and protocol verifications. This paper presents a technique for modeling and analyzing queueing model using stochastic activity networks (SANs). And then some examples are showed for the verification of the technique presented in this paper.