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김기영,박샛별,문지혜,이상대,이새롬,장윤정 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.2
This study was performed to develop a rapid test kit for pathogenic Staphylococcus in various samples. The rapid detection kit has been fabricated based on nitrocellulose lateral-flow strip. Colloidal gold and Staphylococcus antibodies were used as a tag and a receptor, respectively. Manually spotted Staphylococcus antibody and anti-mouse antibody on the surface of nitrocellulose membrane were used as test and control lines, espectively. Feasibility of the rapid kit to detect Staphylococcus aureus in samples were evaluated. The intensity of the color of the tes line started to increase with the samples in which higher concentration of the cells were contained. The sensitivity of the sensor was 106 cfu/mL Staphylococcus spiked in PBS. Also, the rapid test kit could detect 105 cfu/mL of Staphylococcus in chicken meat extract.
Evaluation of Anti-allergy and Anti-oxidative Effects of Green tea Cultivar Extracts
Saet Byeol Park(박샛별),Doo Gyung Moon(문두경),Hyung Don Kim(김형돈),Je Hun Choi(최재훈),Kyung Hae Soe(서경혜),Gwi Yeong Jang(장귀영),Jeong Hoon Lee(이정훈),Dong Hwi Kim(김동휘),Seung Eun Lee(이승은) 한국약용작물학회 2018 한국약용작물학회 학술대회논문집 Vol.2018 No.2
Isolation of Early Neurogenesis Genes with Xenopus cDNA Microarray
Saet-Byeol Yu,Ok-Joo Lee,Young-Ju Park,Sung-Young Lee,Seung-Hwan Lee,Jaeho Yoon,Yoo-Seok Hwang,Jong-Il Kim,Jae-Yong Lee,Jae-Bong Park,Sung Chan Kim,Jaebong Kim 한국실험동물학회 2010 Laboratory Animal Research Vol.26 No.1
Neurogenesis is the process that develops neuroectoderm from ectoderm. Bone morphogenetic protein (BMP) inhibition in ectodermal cells is necessary and sufficient for neurogenesis in Xenopus embryos. To isolate genes involved in early neurogenesis, Xenous Affymetrix gene chips representing 14,400 genes were analyzed in early stage of neuroectodermal cells that were produced by inhibition of BMP signaling with overexpression of a dominant-negative receptor. We identified 265 candidate genes including 107 ESTs which were newly expressed during the early neurogenesis by blocking BMP signaling. The candidates of 10 ESTs were selected and examined for upregulation in neuroectoderm. Five EST genes were confirmed to be upregulated in neuroectoderm and examined for time-dependent expression patterns in intact embryos. Two EST genes were cloned and identified as a homology of CYP26c (Xl.1946.1.A1_at) and Kielin containing VWC domain (Xl.15853.1.A1_at). One of them, CYP26c, was further characterized for its transcriptional regulation and role of anterior-posterior patterning during neurogenesis. Taken together, we analyzed and characterized genes expressed in early neurogenesis. The results suggest that neurogenesis by inhibition of BMP provides useful system to isolate genes involved in early events of neurogenesis during early vertebrate embryogenesis.
Altered PLCβ-1 expression in the gerbil hippocampal complex following spontaneous seizure
( Saet Byeol Lee ),( Yun Jung Oh ),( Jae Kwang Chung ),( Ji Heon Jeong ),( Sang Duk Lee ),( Dae Kyoon Park ),( Kyung Ho Park ),( Jeong Sik Ko ),( Duk Soo Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.9
Although the phospholipase C (PLC)β-1 isoform is associated with spontaneous seizure and distinctively expressed in the telencephalon, the distribution of PLCβ-1 expression in the epileptic gerbil hippocampus remains controversial. Therefore, we determined whether PLCβ-1 is associated with spontaneous seizure in an animal model of genetic epilepsy. In the present study, PLCβ-1 immunoreactivity was down-regulated in seizure-sensitive (SS) gerbils more than in seizure-resistant (SR) gerbils. The expression of PLCβ-1 within calretinin (CR)-positive neurons was rarely detected within the dentate hilar region of SS gerbils. PLCβ-1 immunoreactivity in the hippocampus was significantly elevated as compared to that in pre-seizure SS gerbil 3 h post-ictal. These findings suggest that alterations in PLCβ-1 immunoreactivity in the SS gerbil hippocampus may be closely related to the epileptic state of the gerbil brain and transiently elevated PLCβ-1 protein levels following seizure episodes. Such alterations may be compensatory responses in the SS gerbil hippocampus. [BMB reports 2011; 44(9) : 566-571]
Salmonella 신속 검출용 간이진단키트의 성능 개선 연구
박샛별 ( Saet Byeol Park ),김기영 ( Gi Young Kim ),문지혜 ( Ji Hea Moon ) 한국산업식품공학회 2012 산업 식품공학 Vol.16 No.4
In this study, a rapid immuno-chromatographic assay was developed to detect Salmonella Typhimurium, This assay was based on the lateral flow immunoassay, Two different anti-Salmonella polyclonal antibody (Pab) are used in this strip assay for the double antibody sandwich immunoassay method, The strip sensor was composed of sample pad, conjugation pad, membrane, and absorbent pad. For the optimal conjugation between nano-gold and anti-Salmonella Pab, the pH value of colloidal gold solution was determined to 8.4, To prevent non-specific binding between nano gold and anti-Salmonella Pab, sample pad and membrane was treated with PBS buffer, included 2% BSA and 10% sucrose, The control and detection zones were visually detected according to antigen-antibody binding. The detection limit of the developed strip assay was 105CFU/mL within 10 min, and improved until 104CFU/mL after 12 hr.
A large-scale screening analysis for the evaluation of Bakanae disease in rice
Myung-Hee Kim,Saet-Byeol Lee,Tackmin Kwon,Un-Ha Hwang,Soo-Kwon Park,Yeong-Nam Youn,Jong-Hee Lee,Jun-Hyun Cho,Dongjin Shin,Sang-Ik Han,Un-Sang Yeo,You-Chun Song,Min-Hee Nam,Dong-Soo Park 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Bakanae disease of rice, caused by Fusarium moniliforme Sheldon, the imperfect stage of Gibberella fujikuroi, is one of the most important rice diseases worldwide, but no rice variety has been found to be completely resistant to this fungus. Cultivation of resistant cultivars is the most beneficial way of reducing quantitative or qualitative losses to for bakanae disease in rice. To facilitate the study of this disease, accurate and large scale screening methods were developed for the inoculation and evaluation of Bakanae disease. Even and large scale infection was achieved by using F. moniliforme spore in tissue embedding cassette and seedling tray. The efficiency of F. moniliforme infection with the concentration of 1×106 spore/ml caused better distribution (F-value=33.96) than 1×102 (F-value=10.69), and 1×104 spore/ml (F-value=2.63). We established new criteria of healthy and non-healthy plant, and introduced calculation of proportion of healthy plants to meet fast evaluation of resistance level of each variety. The effect of F. moniliforme strains containing different genetic background was also evaluated with rice varieties to figure out the stability of resistance level. GA3 response of rice variety was significantly correlated with bakanae disease, but it did not adequate for direct indicator of bakanae disease resistance. These results indicated that a large scale infection method developed in this study is fast and reproducible, as well as a disease evaluation system provides an accurate measurement of bakanae disease resistance of rice.