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Lipopeptides Extract from Bacillus Amyloliquefaciens Induce Human Oral Squamous Cancer Cell Death
Kuo, Chen-Hui,Lin, Yun-Wei,Chen, Ruey-Shyang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.1
A lipopeptide extract of Bacillus amyloliquefaciens BACY1 (BLE) was found to induce cell death in human oral squamous cell carcinoma (OSCC) cell lines, SCC4 and SCC25, in this study. The results of MTT assay showed that BLE inhibited OSCC cell proliferation in a dose-dependent manner. BLE was also effective in increasing the sub-G1 phases. Furthermore, when membrane damage in SCC4 cells treated with BLE was monitored by LDH assay, release of LDH was significantly increased. The protein and mRNA levels of pro-apoptotic Bax, and caspase-3 were up-regulated by BLE. Taken together, these results suggest that BLE induces apoptosis and then inhibits the cell proliferation of human OSCC cells.
Investigate the Mechanical and Biological Activities of PVA Membranes
( Chih-chang Yeh ),( Kai-chen Hu ),( Tsung-yan Tsai ),( Chu-wen Shao ),( Ruey-shyang Chen ),( Hsin-i Chang ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
Polyvinyl alcohol (PVA) is a water-soluble synthetic polymer and which has high tensile strength and flexibility, low toxicity and good biocompatibility for the application of soft tissue engineering. Generally, PVA can be crosslinked to form 3-D structure by a variety of methods, such as chemical crosslinking, irradiation or the freeze-thaw techniques. In contrast, chemical-crosslinked PVA structure has good elasticity, high water content, and stability at room temperature but most of crosslinking reagents such as glutaradehyde have high cytotoxicity. In here, we used low toxic genipin as crosslinking reagent for the formulation of PVA membrane. Thermal behavior and mechanical properties of genipin-crosslinked PVA membrane have been measured by thermo gravimetric analyzer and universal testing machine. Genipin-crosslinked PVA membrane had a smooth surface and the contact angel was about 67°. The swelling test displayed that genipin-crosslinked PVA membrane had high water content (130%) and the increase of PVA molecular weight can slightly improve water content. Moreover, dried genipin-crosslinked PVA membrane presented higher tensile strength (7.21MPa) but lower ductility (3.4%) than hydrated membrane (3.9MPa and 302.4%). Interestingly, rehydration of dried PVA membrane can maintain similar ductility to original hydrated membrane but the tensile strength was reduced by 40%. Therefore, drying process may break some hydrogen bonding between the polymer structure of PVA membrane. In MTT assay, there was some toxicity remained in genipin-PVA membrane but cell survivability was 1.5-1.9 fold higher than glutaradehyde-crosslinked PVA membrane. Overall, genipin-crosslinked PVA membrane showed low toxicity, good cell attachment and growth of human foreskin fibroblasts. After the cell culture study, the degradation of genipin-crosslinked PVA membrane was investigated by scanning electron microscope. Therefore, genipin-crosslinked PVA membrane is a biodegradable material and which can apply in soft tissue engineering due to its good elastic behavior.