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Krishnamoorthy, R.,Kim, K.,Subramanian, P.,Senthilkumar, M.,Anandham, R.,Sa, T. Elsevier 2016 Agriculture, ecosystems & environment Vol.231 No.-
Arbuscular mycorrhizal fungi (AMF) have close association with bacteria in establishing a tripartite interaction with plants. The objective of this study was to assess the tripartite interactions among AMF, associated bacteria and maize on enhancing salt stress tolerance. AMF spores isolated from coastal reclamation land were identified as Rhizophagus intraradices and multiplied using monosporic mass culturing. From surface-decontaminated spores of R. intraradices, isolation of associated bacteria was carried out and the obtained isolate was identified as Massilia sp. RK4. The isolated bacterial strain was found to possess several plant growth promoting characteristics and for further studies, its effect on maize plant growth in coastal reclamation soil was evaluated under three different salt concentrations. Salt stress substantially reduced plant growth, root colonization and spore-producing ability of the R. intraradices. However, treatment with the AMF and a combination of AMF and associated bacteria alleviated the salt-induced reduction of plant growth, root colonization, nutrient accumulation and lowered leaf proline levels compared to control treatment. The co-inoculation of R. intraradices and Massilia sp. RK4 exhibited significant impact on AMF root colonization and nutrient accumulation in plants compared to inoculation with R. intraradices only. Inoculation of R. intraradices and Massilia sp. RK4 improved the salinity tolerance of maize through the dual effect exerted by AMF and its associated bacteria.
Krishnamoorthy, R.,Kim, K.,Kim, C.,Sa, T. Elsevier Science B.V., Amsterdam. 2014 Soil biology & biochemistry Vol.72 No.-
A comprehensive knowledge on the relationship between soil salinity and arbuscular mycorrhizal fungi (AMF) is vital for a deeper understanding of ecosystem functioning under salt stress conditions. The objective of this study was to determine the effects of soil salinity on AMF root colonization, spore count, glomalin related soil protein (GRSP) and community structure in Saemangeum reclaimed land, South Korea. Soil samples were collected and grouped into five distinct salt classes based on the electrical conductivity of soil saturation extracts (ECse). Mycorrhizal root colonization, spore count and GASP were measured under different salinity levels. AMF community structure was studied through three complementary methods; spore morphology, terminal restriction fragment length polymorphism (T-RFLP) and denaturing gradient gel electrophoresis (DGGE). Results revealed that root colonization (P < 0.01), spore count (P < 0.01) and GRSP (P < 0.01) were affected negatively by soil salinity. Spore morphology and T-RFLP data showed predominance of AMF genus Glomus in Saemangeum reclaimed land. T-RFLP and DGGE analysis revealed significant changes in diversity indices between non (ECse < 2 dS/m) and extremely (ECse > 16 dS/m) saline soil and confirmed dominance of Glomus caledonium only in soils with ECse < 8 dS/m. However, ribotypes of Glomus mosseae and Glomus proliferum were ubiquitous in all salt classes. Combining spore morphology, T-RFLP and DGGE analysis, we could show a pronounced effect in AMF community across salt classes. The result of this study improve our understanding on AMF activity and dominant species present in different salt classes and will substantially expand our knowledge on AMF diversity in reclaimed lands. (C) 2014 Elsevier Ltd. All rights reserved.
Comparative structural analysis of lattice hybrid and tubular wind turbine towers
R. Kumaravel,A. Krishnamoorthy 한국풍공학회 2020 Wind and Structures, An International Journal (WAS Vol.30 No.1
This paper presents a comparative structural analysis of lattice hybrid tower with six legs with conventional tubular steel tower for an onshore wind turbine using finite element method. Usually a lattice hybrid tower will have a conventional industry standard \'L\' profile section for the lattice construction with four legs. In this work, the researcher attempted to identify and analyze the strength of six legged lattice hybrid tower designed with a special profile instead of four legged L profile. And to compare the structural benefits of special star profile with the conventional tubular tower. Using Ansys, a commercial FEM software, both static and dynamic structural analyses were performed. A simplified finite element model that represents the wind turbine tower was created using Shell elements. An ultimate load condition was applied to check the stress level of the tower in the static analysis. For the dynamic analysis, the frequency extraction was performed in order to obtain the natural frequencies of the tower.