Flowing with Jeju heanyeo - Jeju National University High school club Tewak is floating

Hong Ji Hee,Kang Gyeong Ji,Kim Eun Hee,Kim On Yu,Lee Yu Bin,Ko Jun Hyeok,Kang Min Jae,Yang Hyeon Jeong,Yang Yeon Jae,Sim Yu Rim,Kim Min Ji,Geum Na Hyeon,Kim Geum Byeol,Heo Jeong In 제주학회 2016 제주학회 학술발표논문집 Vol.2016 No.2

Aurora kinase A induces migration and invasion by inducing epithelial-to-mesenchymal transition in colon cancer cells

On-Yu Hong,Sang Yull Kang,Eun-Mi Noh,Hong-Nu Yu,Hye-Yeon Jang,Seong-Hun Kim,Jingyu Hong,Eun Yong Chung,Jong-Suk Kim 생화학분자생물학회 2022 BMB Reports Vol.55 No.2

Aurora kinase is a family of serine/threonine kinases intimatelyassociated with mitotic progression and the development ofhuman cancers. Studies have shown that aurora kinases areimportant for the protein kinase C (PKC)-induced invasion ofcolon cancer cells. Recent studies have shown that aurora kinaseA promotes distant metastasis by inducing epithelial-to-mesenchymaltransition (EMT) in colon cancer cells. However, the roleof aurora kinase A in colon cancer metastasis remains unclear. In this study, we investigated the effects of aurora kinase A onPKC-induced cell invasion, migration, and EMT in human SW480colon cancer cells. Treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) changed the expression levels of EMT markers,increasing α-SMA, vimentin, and MMP-9 expression and decreasingE-cadherin expression, with changes in cell morphology. TPA treatment induced EMT in a PKC-dependent manner. Moreover,the inhibition of aurora kinase A by siRNAs and inhibitors(reversine and VX-680) suppressed TPA-induced cell invasion,migration, and EMT in SW480 human colon cells. Inhibition ofaurora kinase A blocked TPA-induced vimentin and MMP-9 expression,and decreased E-cadherin expression. Furthermore, theknockdown of aurora kinase A decreased the transcriptionalactivity of NF-κB and AP-1 in PKC-stimulated SW480 cells. Thesefindings indicate that aurora kinase A induces migration andinvasion by inducing EMT in SW480 colon cancer cells. To thebest of our knowledge, this is the first study that showed aurorakinase A is a key molecule in PKC-induced metastasis in coloncancer cells.

Epigallocatechin gallate inhibits the growth of MDA-MB-231 breast cancer cells via inactivation of the β-catenin signaling pathway

Hong, On-Yu,Noh, Eun-Mi,Jang, Hye-Yeon,Lee, Young-Rae,Lee, Byoung Kil,Jung, Sung Hoo,Kim, Jong-Suk,Youn, Hyun Jo Spandidos Publications 2017 Oncology letters Vol.14 No.1

DHA blocks TPA-induced cell invasion by inhibiting MMP-9 expression via suppression of the PPAR-γ/NF-κB pathway in MCF-7 cells

Hwang, Jin-Ki,Yu, Hong-Nu,Noh, Eun-Mi,Kim, Jeong-Mi,Hong, On-Yu,Youn, Hyun Jo,Jung, Sung Hoo,Kwon, Kang-Beom,Kim, Jong-Suk,Lee, Young-Rae D.A. Spandidos 2017 Oncology letters Vol.13 No.1

<P>Docosahexaenoic acid (DHA) is an omega-3 fatty acid that is considered to have applications in cancer prevention and treatment. The beneficial effects of DHA against cancer metastasis are well established; however, the mechanisms underlying these effects in breast cancer are not clear. Cell invasion is critical for neoplastic metastasis, and involves the degradation of the extracellular matrix by matrix metalloproteinase (MMP)-9. The present study investigated the inhibitory effect of DHA on MMP-9 expression and cell invasion induced by 12-<I>O</I>-tetradecanoylphorbol-13-acetate (TPA) in the MCF-7 breast cancer cell line. DHA inhibited the TPA-induced activation of mitogen-activated protein kinase (MAPK) and the transcription of nuclear factor (NF)-κB, but did not inhibit the transcription of activator protein-1. DHA increased the activity of peroxisome proliferator-activated receptor (PPAR)-γ, an effect that was reversed by the application of the PPAR-γ antagonist GW9662. In addition, combined treatment with GW9662 and DHA increased NF-κB-related protein expression. These results indicate that DHA regulates MMP-9 expression and cell invasion via modulation of the MAPK signaling pathway and PPAR-γ/NF-κB activity. This suggests that DHA could be a potential therapeutic agent for the prevention of breast cancer metastasis.</P>

최근 10년 동안 일개 상급종합병원의 칸디다혈증 환자에서 분리된 칸디다 균종의 빈도

Yu-Yean Hwang,On-Kyun Kang,Chang-Eun Park,Sung-No Hong,Young-Kwon Kim,Hee-Jae Huh,Nam-Yong Lee 대한임상검사과학회 2022 대한임상검사과학회지(KJCLS) Vol.54 No.2

Candidemia is a major cause of nosocomial infections resulting in increased morbidity and mortality. It remains a serious risk in inpatients and increases medical treatment costs. From 2009 to 2018, Candida strains (3,533) isolated from blood culture tests at the S Hospital were analyzed according to the period, year, sex, age, ward, etc. During the entire period, 54,739 of 717,996 blood culture tests showed a positive rate (7.6%) and the Candida isolation rate was 3,533 (6.4%) out of 1,036 patients. Among the Candida isolates, C. albicans was most common (33.8%), followed by C. tropicalis (28.6%), C. glabrata (19.8%), C. parapsilosis (7.8%), and C. krusei (4.0%). In early (2009∼2013)/late (2014∼2018) isolation, C. tropicalis decreased by 3.8% and C. glabrata increased by 3.4%. After 50 years of age, the higher the separation frequency. C. parapsilosis (31.3%) in 1∼10s, C. tropicalis (30.3%) and C. glabrata (27.6%) in 41∼50s, and C. tropicalis (28.6%) in 80s are relatively frequent. has been separated C. krusei was isolated in a relatively high proportion from females (60.9%). Therefore, a systematic and continuous nosocomial infection control system should be established for appropriate treatment as per antifungal treatment guidelines. The system should continuously monitor the distribution of Candida species and provide rapid identification results. 칸디다혈증(candidemia)은 이환율과 사망률을 높이는 주요 원인으로 입원 환자에서 심각한 위험으로 남아 있으며 의료비용을 증가시킨다. 2009년부터 2018년까지 S병원의 혈액 배양 검사에서 분리된 칸디다 균주 3,533건을 대상으로 시기별 분리빈도, 연도, 성별, 나이, 병동 등에 따라 분석하였다. 전체 기간 중 혈액 배양 의뢰 건수 717,996 중에 54,739건이 배양 양성으로 7.6%의 양성율을 보였으며 칸디다 분리률은 1,036명의 환자에서 3,533건으로 6.4%였다. 균종의 분포는 C. albicans (33.8%), C. tropicalis (28.6%), C. glabrata (19.8%), C. parapsilosis (7.8%), C. krusei (4.0%) 이다. 전기/후기 분리에서는 C. tropicalis가 3.8% 감소하고 C. glabrata 는 3.4% 증가하였다. 50세 이후 연령이 증가할수록 분리 빈도가 높았으며, 1∼10대에서는 C. parapsilosis (31.3%), 41∼50 대에서는 C. tropicalis (30.3%), C. glabrata (27.6%) 순으로, 80대에서는 C. tropicalis (28.6%)가 상대적으로 자주 분리되었다. C. krusei 는 여성(60.9%)에서 상대적으로 높은 비율로 분리되었다. 따라서 Candida 균종의 분포를 지속적으로 감시하고 신속한 동정 결과를 제공하여 적절한 치료 및 항진균제 치료 지침을 위한 체계적이고 지속적인 병원감염관리 시스템이 이루어져야 할 것이다.

Neutrophil-to-lymphocyte ratio independently predicts advanced pathological staging and poorer survival outcomes in testicular cancer

Yu Guang Tan,Joshua Sia,Hong Hong Huang,Weber Kam On Lau 대한비뇨의학회 2019 Investigative and Clinical Urology Vol.60 No.3

Purpose: An elevated neutrophil-to-lymphocyte ratio (NLR) has been associated with adverse outcomes in various malignancies. However, its role in prognosticating testicular cancer (TC) has not been validated. We aim to study the relationship between NLR and TC. Materials and Methods: We retrospectively reviewed 160 patients with histological proven TC from January 2005 to June 2016. Youden's index was used to analyse NLR and a cut-off point of 3.0 was obtained, with statistical receiver operating characteristics of 0.755. Chi-square test, Kaplan-Meier (log rank test) and logistics regression models were used to predict NLR association with survival outcomes. Results: Median age was 34 years old (range, 17–68 years old). There were 102 pure seminomas and 58 non-seminomatous germ cell tumours. Median follow-up period was 8 years (range, 2.5–17 years). NLR ≥3.0 was independently associated with lymph node involvement (p=0.031; odds ratio [OR], 2.91; 95% confidence interval [CI], 1.67–5.83; p=0.038; OR, 4.12; 95% CI, 1.26–6.51) and metastatic disease (p=0.041; OR, 2.48; 95% CI, 1.22–3.98; p=0.043; OR, 2.21; 95% CI, 1.17–3.65) in both seminomatous and non-seminomatous germ cell tumours, translating to a more advanced disease. Moreover, NLR ≥3.0 also predicts poorer cancer specific survival in these patients. Conclusions: NLR can be an inexpensive haematological marker in predicting advanced TC staging and poorer survival outcome. NLR complements the traditional cancer staging by identifying a group of high risk patients who may benefit from multimodal treatment and closer surveillance to achieve long term survival.

15d-PGJ2 inhibits NF-kB and AP-1-mediated MMP-9 expression and invasion of breast cancer cell by means of a heme oxygenase-1-dependent mechanism

장혜연,On-Yu Hong,Hyun Jo Youn,김민걸,Cheorl-Ho Kim,정성후,Jong-Suk Kim 생화학분자생물학회 2020 BMB Reports Vol.53 No.4

Activation of peroxisome proliferator-activated receptor  (PPAR) serves as a key factor in the proliferation and invasion of breast cancer cells and is a potential therapeutic target for breast cancer. However, the mechanisms underlying this effect remain largely unknown. Heme oxygenase-1 (HO-1) is induced and overexpressed in various cancers and is associated with features of tumor aggressiveness. Recent studies have shown that HO-1 is a major downstream target of PPAR. In this study, we investigated the effects of induction of HO-1 by PPAR on TPAinduced MMP-9 expression and cell invasion using MCF-7 breast cancer cells. TPA treatment increased NF-B /AP-1 DNA binding as well as MMP-9 expression. These effects were significantly blocked by 15d-PGJ2, a natural PPAR ligand. 15d-PGJ2 induced HO-1 expression in a dose-dependent manner. Interestingly, HO-1 siRNA significantly attenuated the inhibition of TPA-induced MMP-9 protein expression and cell invasion by 15d-PGJ2. These results suggest that 15d-PGJ2 inhibits TPA-induced MMP- 9 expression and invasion of MCF-7 cells by means of a heme oxygenase-1-dependent mechanism. Therefore, PPAR/HO-1 signaling- pathway inhibition may be beneficial for prevention and treatment of breast cancer.

수열합성법을 이용한 망간 나노와이어 제조 및 이의 전기화학적 특성 연구

홍석복 ( Seok Bok Hong ),강온유 ( On Yu Kang ),황성연 ( Sung Yeon Hwang ),허영민 ( Young Min Heo ),김정원 ( Jung Won Kim ),최봉길 ( Bong Gill Choi ) 한국공업화학회 2016 공업화학 Vol.27 No.6

본 연구에서는 1차원의 MnO₂ nanowire를 KMnO₄와 MnSO₄ 전구체 혼합물의 수열합성법(hydrothermal method)을 사용하여 제조할 수 있는 합성법을 개발하였다. 제조된 MnO₂ nanowire는 전기화학 반응 동안 전자와 이온전달을 용이하게 할 수 있는 넓은 비표면적과 기공구조를 나타내었다. MnO₂ nanowire의 미세구조 및 화학구조를 주사형 전자현미경(SEM), 투과전자현미경(TEM), 광전자분석기(XPS), X-ray 회절분석법(XRD), 비표면적분석장비(BET)를 사용하여 분석하였다. 본 MnO₂ nanowire 전극의 전기화학적 특성은 순환전압전류법(cyclic voltammetry)과 정전류 충전-방전법(galvanostatic charge-discharge)을 사용하여 3상 전극 시스템(three-electrode system)에서 분석하였다. MnO₂ nanowire 전극은 높은 비정전용량(129 F/g), 고속 충방전(61% retention), 반 영구적인 수명특성(100%)을 나타내었다. In this work, we developed a synthetic method for preparing one-dimensional MnO₂ nanowires through a hydrothermal meth-od using a mixture of KMnO₄ and MnSO₄ precursors. As-prepared MnO₂ nanowires had a high surface area and porous struc-ture, which are beneficial to the fast electron and ion transfer during electrochemical reaction. The microstructure and chemical structure of MnO₂ nanowires were characterized by scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, X-ray diffraction, and Brunauer-Emmett-Teller measurements. The electrochemical properties of MnO₂ nanowire electrodes were also investigated using cyclic voltammetry and galvanostatic charge-discharge with a three-electrode system. MnO₂ nanowire electrodes showed a high specific capacitance of 129 F/g, a high rate capability of 61% retention, and an excellent cycle life of 100% during 1000 cycles.

Jowiseungchungtang Inhibits Amyloid-β Aggregation and Amyloid-β-Mediated Pathology in 5XFAD Mice

Shin, Soo Jung,Jeong, Yu-on,Jeon, Seong Gak,Kim, Sujin,Lee, Seong-kyung,Nam, Yunkwon,Park, Yong Ho,Kim, Dabi,Lee, Youn Seok,Choi, Hong Seok,Kim, Jin-il,Kim, Jwa-Jin,Moon, Minho MDPI AG 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.12

<P>Alzheimer’s disease (AD) is a neurodegenerative disease, which is accompanied by memory loss and cognitive dysfunction. Although a number of trials to treat AD are in progress, there are no drugs available that inhibit the progression of AD. As the aggregation of amyloid-β (Aβ) peptides in the brain is considered to be the major pathology of AD, inhibition of Aβ aggregation could be an effective strategy for AD treatment. Jowiseungchungtang (JWS) is a traditional oriental herbal formulation that has been shown to improve cognitive function in patients or animal models with dementia. However, there are no reports examining the effects of JWS on Aβ aggregation. Thus, we investigated whether JWS could protect against both Aβ aggregates and Aβ-mediated pathology such as neuroinflammation, neurodegeneration, and impaired adult neurogenesis in 5 five familial Alzheimer’s disease mutations (5XFAD) mice, an animal model for AD. In an in vitro thioflavin T assay, JWS showed a remarkable anti-Aβ aggregation effect. Histochemical analysis indicated that JWS had inhibitory effects on Aβ aggregation, Aβ-induced pathologies, and improved adult hippocampal neurogenesis in vivo. Taken together, these results suggest the therapeutic possibility of JWS for AD targeting Aβ aggregation, Aβ-mediated neurodegeneration, and impaired adult hippocampal neurogenesis.</P>

A novel method for the management of proximal segment using computer assisted simulation surgery: correct condyle head positioning and better proximal segment placement

Lee, Yong-Chan,Sohn, Hong-Bum,Kim, Sung-Keun,Bae, On-Yu,Lee, Jang-Ha Korean Association of Maxillofacial Plastic and Re 2015 Maxillofacial Plastic Reconstructive Surgery Vol.37 No.-

Computer Assisted Simulation Surgery (CASS) is a reliable method that permits oral and maxillofacial surgeons to visualize the position of the maxilla and the mandible as observed in the patient. The purpose of this report was to introduce a newly developed strategy for proximal segment management according to Balanced Orthognathic Surgery (BOS) protocol which is a type of CASS, and to establish the clinical feasibility of the BOS protocol in the treatment of complex maxillo-facial deformities. The BOS protocol consists of the following 4 phases: 1) Planning and simulation phase, 2) Modeling phase, 3) Surgical phase, and 4) Evaluation phase. The surgical interventions in 80 consecutive patients were planned and executed by the BOS protocol. The BOS protocol ensures accuracy during surgery, thereby facilitating the completion of procedures without any complications. The BOS protocol may be a complete solution that enables an orthognatic surgeon to perform accurate surgery based on a surgical plan, making real outcomes as close to pre-planned outcomes as possible.