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Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression
Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11
<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>
Factors influencing choosing teaching as a career: South Korean preservice teachers
Jeong‑Ae Lee,Mi Ok Kang,Bitnara Jasmine Park 서울대학교 교육연구소 2019 Asia Pacific Education Review Vol.20 No.3
This study validated the underlying factor structure of the Factors Influencing Teaching (FIT)-Choice survey in the South Korea context and examined motivations and perceptions of 903 Korean preservice teachers by major demographic characteristics. Secondary preservice teachers reported significantly higher values than elementary preservice teachers for Qualification fit and desire to teach and Task return. Male preservice teachers believed more strongly than female preservice teachers that they chose the teaching profession because of Qualification fit, Influencing others, and Task return. Compared to freshmen/sophomores, juniors/seniors reported significantly higher values for Task demand. Participants with low- to medium-level self-reported income ($12K–$46K) weighted Benefits significantly higher than those with medium-level income ($46K–$88K). STEM majors reported higher values for the Qualification fit and Influencing others than non-STEM majors. As South Korea is one of the few countries which has an oversupply of highly qualified teacher candidates, findings were compared with other countries to provide useful insights to improve recruitment of highly qualified individuals to teaching workforce in many countries where teacher shortage is a chronic problem. Based on these data analyses, we argue that policy makers, politicians, education researchers, and others who care about quality education and student academic achievements need to make efforts to increase task returns and benefits.
Alcohol Fermentation Characteristics of the Korean Native Mulberry (Morus spp.)
Ok Mi Kim,Seung-Mi Woo,Yong Kon Park,Yong-Jin Jeong 한국식품영양과학회 2006 Preventive Nutrition and Food Science Vol.11 No.2
This study was carried out to investigate the fermentation characteristics and optimum conditions for alcohol fermentation of the Korean native mulberry. The yeast strains of S. kluyveri DJ97, La parisienne (Saccharomyces cerevisiae, Netherlands) and Enoferm (Saccharomyces cerevisiae, Denmark) produced higher alcohol concentrations than other strains, and further study was therefore performed with these three species. The optimum additional water content for maximizing alcohol concentration was 250% (v/w). The alcohol concentrations were rapidly increased in the first 4 days under the optimum conditions and reached 13.8% for S. kluyveri DJ97, 14.0% for La parisienne and 14.0% for Enoferm, respectively. Residual sugar concentration was decreased steadily from the beginning of fermentation until 5 days, after which it maintained a constant level. The pH was decreased steadily in the log phase during further maturation. However, the pH underwent a slight decrease after 4 days and then was stabilized during further maturation. Methanol concentrations for the three species used were analyzed after 60 days maturation and were lower than the levels regulated by the food standard. Fusel oils such as n-propanol, iso-butanol, and iso-amyl-alcohol were produced as by-products with the highest production from Enoferm and the lowest from S. kluyveri DJ97.
Jeong, Sang Hyeon,Yang, Sung-Hyun,Jin, Hyun Mi,Kim, Jeong Myeong,Kwon, Kae Kyoung,Jeon, Che Ok International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.4
<P>Two novel Gram-stain-negative, chemoheterotrophic and strictly aerobic bacteria, strains GY2<SUP>T</SUP> and SPO729<SUP>T</SUP>, were isolated from a tidal flat at Gwangyang Bay in Korea and a marine sponge sample from the Pacific Ocean, respectively. The two strains were halotolerant, catalase- and oxidase-positive, and non-motile rods. Optimum temperature and pH for growth of both strains were observed to be 35 °C and pH 7.0–7.5, but optimum salinity for strain SPO729<SUP>T</SUP> [2–3 % (w/v)] was slightly higher than that for strain GY2<SUP>T</SUP> (1–2 %). The major cellular fatty acids of both strains were C<SUB>16 : 0</SUB>, iso-C<SUB>15 : 0</SUB>, iso-C<SUB>17 : 0</SUB>, iso-C<SUB>17 : 1</SUB>ω9<I>c</I>, C<SUB>18 : 1</SUB>ω7<I>c</I>, iso-C<SUB>11 : 0</SUB> and iso-C<SUB>11 : 0</SUB> 3-OH. The genomic DNA G+C contents of strains GY2<SUP>T</SUP> and SPO729<SUP>T</SUP> were 55.1 and 57.9 mol%, respectively, and ubiquinone 8 (Q-8) was detected as the sole respiratory quinone from the two strains. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains GY2<SUP>T</SUP> and SPO729<SUP>T</SUP> formed tight phyletic lineages with members of the genus <I>Microbulbifer</I>. Strain GY2<SUP>T</SUP> was closely related to <I>Microbulbifer okinawensis</I> ABABA23<SUP>T</SUP> (98.2 %), strain SPO729<SUP>T</SUP> (98.0 %) and <I>Microbulbifer donghaiensis</I> CN85<SUP>T</SUP> (97.0 %); strain SPO729<SUP>T</SUP> was closely related to <I>M. okinawensis</I> ABABA23<SUP>T</SUP> (98.3 %) and <I>M. donghaiensis</I> CN85<SUP>T</SUP> (98.2 %). The DNA–DNA relatedness values of strain GY2<SUP>T</SUP> with <I>M. okinawensis</I> ABABA23<SUP>T</SUP>, strain SPO729<SUP>T</SUP> and <I>M. donghaiensis</I> CN85<SUP>T</SUP> were 40.0±2.1 %, 13.1±3.9 % and 16.2±5.8 %, respectively, whereas those of strain SPO729<SUP>T</SUP> with <I>M. okinawensis</I> ABABA23<SUP>T</SUP> and <I>M. donghaiensis</I> CN85<SUP>T</SUP> were 48.0±4.0 % and 34.6±9.3 %, respectively. On the basis of phenotypic and molecular features, it is concluded that the two strains GY2<SUP>T</SUP> and SPO729<SUP>T</SUP> represent two novel species of the genus <I>Microbulbifer</I>, for which the names <I>Microbulbifer gwangyangensis</I> sp. nov. and <I>Microbulbifer pacificus</I> are proposed; the type strains are GY2<SUP>T</SUP> ( = KACC 16189<SUP>T</SUP> = JCM 17800<SUP>T</SUP>) and SPO729<SUP>T</SUP> ( = KCCM 42667<SUP>T</SUP> = JCM 14507<SUP>T</SUP>), respectively.</P>
Jeong, Hae Jin,Lim, An Suk,Lee, Kitack,Lee, Moo Joon,Seong, Kyeong Ah,Kang, Nam Seon,Jang, Se Hyeon,Lee, Kyung Ha,Lee, Sung Yeon,Kim, Mi Ok,Kim, Ji Hye,Kwon, Ji Eun,Kang, Hee Chang,Kim, Jae Seong,Yih, The Korean Society of Phycology 2017 ALGAE Vol.32 No.2
The ichthyotoxic Cochlodinium polykrikoides red tides have caused great economic losses in the aquaculture industry in the waters of Korea and other countries. Predicting outbreak of C. polykrikoides red tides 1-2 weeks in advance is a critical step in minimizing losses. In the South Sea of Korea, large C. polykrikoides red tide patches have often been recorded offshore and transported to nearshore waters. To explore the processes of offshore C. polykrikoides red tides, temporal variations in 3-dimensional (3-D) distributions of red tide organisms and environmental parameters were investigated by analyzing 4,432 water samples collected from 2-5 depths of 60 stations in the South Sea, Korea 16 times from May to Nov, 2014. In the study area, the vegetative cells of C. polykrikoides were found as early as May 7, but C. polykrikoides red tide patches were observed from Aug 21 until Oct 9. Cochlodinium red tides occurred in both inner and outer stations. Prior to the occurrence of large C. polykrikoides red tides, the phototrophic dinoflagellates Prorocentrum donghaiense (Jun 12 to Jul 11), Ceratium furca (Jul 11 to Aug 21), and Alexandrium fraterculus (Aug 21) formed red tides in sequence, and diatom red tides formed 2-3 times without a certain distinct pattern. The temperature for the optimal growth of these four red tide dinoflagellates is known to be similar. Thus, the sequence of the maximum growth rates of P. donghaiense > C. furca > A. fraterculus > C. polykrikoides may be partially responsible for this sequence of red tides in the inner stations following high nutrients input in the surface waters because of heavy rains. Furthermore, Cochlodinium red tides formed and persisted at the outer stations when $NO_3$ concentrations of the surface waters were < $2{\mu}M$ and thermocline depths were >20 m with the retreat of deep cold waters, and the abundance of the competing red-tide species was relatively low. The sequence of the maximum swimming speeds and thus potential reachable depths of C. polykrikoides > A. fraterculus > C. furca > P. donghaiense may be responsible for the large C. polykrikoides red tides after the small blooms of the other dinoflagellates. Thus, C. polykrikoides is likely to outgrow over the competitors at the outer stations by descending to depths >20 m and taking nutrients up from deep cold waters. Thus, to predict the process of Cochlodinium red tides in the study area, temporal variations in 3-D distributions of red tide organisms and environmental parameters showing major nutrient sources, formation and depth of thermoclines, intrusion and retreat of deep cold waters, and the abundance of competing red tide species should be well understood.
Jeong, Lak Shin,Yoo, Su Jeong,Lee, Kang Man,Koo, Mi Jeong,Choi, Won Jun,Kim, Hea Ok,Moon, Hyung Ryong,Lee, Min Young,Park, Jae Gyu,Lee, Sang Kook,Chun, Moon Woo 梨花女子大學校 藥學硏究所 2003 藥學硏究論文集 Vol.- No.12
Fluoroneplanocin A (12) was designed as a novel mechanism-based inhibitor of S-adenosylhomocysteine hydrolase (SAH) and efficiently synthesized via an electrophilic vinyl fluorination reaction (n-BuLi, N-fluorobenzenesulfonimide at -78℃). Fluoroneplanocin A exhibited 2-fold more potent SAH Inhibitory activity than the parent neplanocin A. A new mechanism of irreversible Inhibition discovered in this work might provide new alternatives in the design of a different class of antiviral agents operating via SAH inhibition.