대장균 Superoxide dismutase에 대한 Paraquat의 호기적 활성저해

강주화,류옥림 효성여자대학교 가정대학 학도호국단 1986 家政大論集 Vol.5 No.-

Turbidometric growth of the Escherisia Coli was drastically inhibited in the presenceof 0.3M paraauat and their growth did not recovered within four hours of test. By the treatment the cells showed only a 30percent of survival when they were coltivated with shaking while they showed 60percent of survival with stationary cultivation. On contrast to it, the oxygen solubilized in the cultural medium was almost same as 30 to 35ppm in the both of shaking and stationary cultural syotem, Activity of superoxidedismutase of the shaking cultured cells was 52.0to 52.9percent against that of control,While activity of the enzyme in stationary cultured cells was 86.2 to 82.7percent.

C_3 및 C_7위치에 치환된 새로운 세파로스포린 化合物의 合成과 抗菌活性(Ⅰ)

황화영,하재천,김영수,고옥현,강형룡 조선대학교 약학연구소 1994 藥學硏究誌 Vol.16 No.1

The synthesis and antimicrobial activity of cephalosporin having (Z)-2-(2-aminothiazol-4-yl)-2-trityloxyiminoacetamido group and 5-(2-chlorophenyl)-4-phenyl-4H-1, 2, 4-triazol-3-thiomethyl group on the C_3 and C_7 position of the cephem ring, respectively are descrived. This compound was tested for antimicrobial activity in vitro against Candida albicans ATCC 10231, Bacillus subtilis ATCC 6633, Micrococcus luteus ATCC 9341, Staphylococcus aureus ATCC 6538P, Mycobacterium phlei IIP-IPH, Escherichia coli KCTC 1039, Escherichia coli ESS, Klebisiella pneumoniae KCTC 1560, Salmonella typhymurium TV-119, Pseudomonas aeruginosa IFO 13130. The antimicrobial activity of synthetic compound was better as compare with cefotaxime and cefazoline against Micrococcus luteus ATCC 9341 & Escherichia coli KCTC ESS.

베타락탐계 항생제 합성과 항균성

고옥현,강형룡,유진철,김경수,홍석순,김영수,황화영,하재천 조선대학교 약학연구소 1992 藥學硏究誌 Vol.14 No.1

7β[5-(Substituted) phenyl-2H-tetrazol-2-yl]acetamidocephalospranic acid, 7β-(5-Diphnenyl-methyl-2H-tetrazol-2-yl) acetamidocephalosporanic acid and 7β-[5-(Substituted)-4-phenyl-1.2.4-triazol2-yl]thioacetamidocephalosporanic acid were synthesized and tested in vitro antimicrobial activity. These compounds exhibited good antimicrobial activity against Gram-positive bacteria whereas most compounds showed decreased antimicrobial activity against Gram-negative bacteria

HL-60 세포주를 이용한 결핵균항원의 세포성면역반응의 분석

박정규,강윤중,김운옥,임재현,송창화,조은경,김화중 충남대학교 의학연구소 2001 충남의대잡지 Vol.28 No.2

Most persons who become infected with M. tuberculosis mount a protective immune response and remain clinically well, the only evidence of infection being development of a positive tuberculin skin test. Five to 10% develop tuberculosis disease within the first 2 years after infection (primary tuberculosis) or thereafter (reactivation tuberculosis). Acquired resistance against tuberculosis paradigmatically rests on cell-mediated immunity, with the major factors being mononuclear phagocytes and T Lymphocytes. While the former cells act as the principal effectors, the latter ones serve as the predominant inducers of protection. The usefulness of the single dose of BCG routinely given in childhood in many developing countries in preventing far more commonly occurring tuberculosis in adults is in doubt. An effective and safe vaccine against tuberculosis is sorely needed. A subunit vaccine are capable of inducing protective immunity and could have substantial advantages over BCG or other whole-bacterium vaccines. The human promyelocytic cell line HL-60 adopted characteristic macrophage-like properties, including adherence and CD14 expression after a period of continuous culture at high ambient CO_(2) concentration. When HL-60 cells were cultured with 1,25-dihydroxyvitamin D_(3) for 4 days, the cells acquired the activity to potentiate T cell proliferation by the 30 kDa or 38 kDa antigen of Mycobacterium tuberculosis H37Rv Therefore, vitamin D-treated HL-60 cells showed the function of the antigen presenting cells.

연합대학으로의 대학 체제 변화에 관한 연구

서재천,서강식,조붕환,지옥화,장원순 공주교육대학교 2010 公州敎大論叢 Vol.47 No.1

본 연구는 교육과학기술부의 경쟁력있는 국립대학을 만들고자 시행하고 있는 여러 정책 중 연합대학으로의 체제 변화 권유에 대한 장단점과 이해득실을 냉철하게 객관적으로 고찰해 보고자 수행되었다. 연구의 수행방법은 연합대학 체제의 추진이나 독자생존의 길 등 의사결정에 참고자료가 될 수 있도록 일본과 미국 등 외국의 연합대학 사례를 중심으로 수집 가능한 자료를 수집하여 고찰한 후 연합대학 체제 추진이나 독자생존의 장단점을 알아보았다. 본 연구의 결과는 여러 가지 외적인 상황 속에서 우리 대학이 독자적으로 생존하는 것이 가능한가, 또는 결국에는 독자적 생존의 대안으로 연합 내지는 통합의 길을 선택할 수밖에 없는가 하는 것은 그 누구도 쉽게 예단하기 어렵다는 것이다. 그러나 대학 구성원들이 미래를 선택하지 않는다 하더라도 외적인 요인에 의해 미래가 결정될 여지가 있으므로 지금부터라도 대학의 미래에 관한 공동의 지혜를 모아야 할 것으로 생각한다. 함께 머리를 맞대고 허심탄회하게 서로의 의견을 존중하며 서로에 대한 불신을 털어버리고 진정 대학의 미래를 위한 바른 선택이 무엇인가에 관한 논의와 연구가 꾸준하게 지속되어야 할 것으로 판단된다. This study was carried out in order to try to consider objectively merits and demerits regarding a system change invitation toward the Union University System and understanding gains and losses during a lot of policies to make a national college that there was competitiveness of Ministry of Education, Science and Technology of education. The results of this study is that no one is easy to predict, that it does as alternatives of an independent survival or select a street of integration and the Union University System in various external situations, finally. However, the University members does not select the future even if in various external factor because there is no doubt that the future will be decided from now on any University in the future regarding collaborative wisdom of the university, We thought it was going to do. Head with open-minded to respect each other's opinions and distrust each other confide you discard the truly University for the future, select the right to discuss and research can be sustained.

Vibrio vulnificus에 의한 패혈증 3예

김신무,김유현,신상희,오재세,강숙희,윤여안,이화재,김영옥 대한임상병리사협회 1986 임상병리사회지 Vol.18 No.1

Antibacterial activity of aurcumin against methicillin-resistant staphylococcus aureus

KANG Ok-Hwa,MUN Su-Hyun,KIM Sung-Bae,KEUM Joon-Ho,SEO Yun-Soo,KWON Dong-Yeul 大韓藥學會 2012 大韓藥學會 總會 및 學術大會 Vol.2012 No.1

Citrus unshiu Water Extract Inhibits Trypsin-induced TNF-α and Tryptase Productions by Blocking the ERK Phosphorylation and Trypsin Activity

Ok Hwa Kang,Dae Ki Kim,Young Mi Lee 한국생약학회 2004 Natural Product Sciences Vol.10 No.5

Citrus unshiu (Rutaceae) has long been known as an anti-inflammatory and anti-allergic agent. In the present study, the inhibitory effect of CUWE (Citrus unshiu water extract) on the production of TNF-α and tryptase was examined. In addition, a possible mechanism for the inhibition of trypsin-stimulated human leukemic mast cell-1 (HMC-1) activation was determined. To do so, TNF-α production from the HMC-1 cells that were stimulated by trypsin (100 nM) in the presence or absence of CUWE (10, 100, and 100 μg/ml) was measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-PCR. The tryptase production was evaluated by reverse transcription-PCR. Extracellular signal-regulated kinase (ERK) activation was analyzed by Western blot. Trypsin activity was measured by using Bz-DL-Arg-p-nitroanilide (BAPNA) as substrate. Results showed that the CUWE inhibited production of both TNF-α and tryptase from the trypsin-stimulated HMC-1 in a dose-dependent manner. The CUWE also inhibited the ERK phosphorylation and trypsin activity. These results indicate that the CUWE had an inhibitory effect on TNF-α and the tryptase productions by blocking the ERK phosphorylation and trypsin activity.

Puerarin ameliorates hepatic steatosis by activating the PPARα and AMPK signaling pathways in hepatocytes

KANG, OK-HWA,KIM, SUNG-BAE,MUN, SU-HYUN,SEO, YUN-SOO,HWANG, HYEONG-CHIL,LEE, YOUNG-MI,LEE, HO-SEOB,KANG, DAE-GIL,KWON, DONG-YEUL Spandidos Publications 2015 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.35 No.3

<P>Non-alcoholic fatty liver disease (NAFLD) is characterized by the hepatic manifestation of metabolic syndrome and is the leading cause of chronic liver disease. Steatohepatitis plays a critical role in the process resulting in liver fibrosis and cirrhosis. Puerarin is a herbal product widely used in Asia, and is believed to have therapeutic benefits for alleviating the symptoms of steatohepatitis. The present study was designed to investigate the effects and mechanisms of action of puerarin in reducing lipid accumulation in oleic acid (OA)-treated HepG2 cells. Hepatocytes were treated with OA with or without puerarin to observe lipid accumulation by Oil Red O staining. We also examined hepatic lipid contents (e.g., triacylglycerol and cholesterol) following treatment with puerarin. Western blot analysis and reverse trabscription-polymerase chain reaction (RT-PCR) were used to measure sterol regulatory element binding protein (SREBP)-1, fatty acid synthase (FAS), peroxisome proliferator-activated receptor alpha (PPAR alpha) and adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) protein and mRNA expression, respectively. Our results revealed that puerarin suppressed OA-induced lipid accumulation, and reduced the triacylglycerol and cholesterol levels. Furthermore, puerarin decreased the expression levels of lipogenic enzymes, such as FAS and SREBPs, and increased the expression levels of PPARa, which are critical regulators of hepatic lipid metabolism through the AMPK signaling pathway. These results indicate that puerarin has the same ability to activate AMPK, and reduce SREBP-1 and FAS expression, thus inhibiting hepatic lipogenesis and increasing hepatic antioxidant activity. We found that puerarin exerted a regulatory effect on lipid accumulation by decreasing lipogenesis in hepatocytes. Therefore, puerarin extract may have therapeutic benefits in the treatment of fatty liver and lipid-related metabolic disorders.</P>

Tetrandrine suppresses pro-inflammatory mediators in PMA plus A23187-induced HMC-1 cells

KANG, OK-HWA,AN, HYEON-JIN,KIM, SUNG-BAE,MUN, SU-HYUN,SEO, YUN-SOO,JOUNG, DAE-KI,CHOI, JANG-GI,SHIN, DONG-WON,KWON, DONG-YEUL UNKNOWN 2014 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.33 No.5

Tetrandrine (TET), a bis-benzylisoquinoline alkaloid from the root of Stephania tetrandra, is known to possess antitumor activity in various malignant neoplasms. However, the precise mechanism of TET-mediated immune modulation remains to be clarified. One of the possible mechanisms for its protective properties is by downregulation of the inflammatory responses. In the present study, the human mast cell line (HMC-1) was used to investigate this effect. TET significantly inhibited the induction of inflammatory cytokines such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-8 by phorbol 12-myristate 13-acetate (PMA) plus A23187. Moreover, TET attenuated expression of cyclooxygenase (COX)-2. In activated HMC-1 cells, the phosphorylation of extra-signal response kinase (ERK1/2) and c-jun N-terminal Kinase (JNK1/2), but not p38 mitogen-activated protein kinase, was decreased by treatment of the cells with TET. TET inhibited PMA plus A23187-induced nuclear factor (NF)-kappa B activation, I kappa B degradation and phosphorylation. Furthermore, TET suppressed the expression of TNF-alpha, IL-8, IL-6 and COX-2 through suppression of the ERK1/2, JNK1/2, I kappa B alpha degradation and phosphorylation, and NF-kappa B activation. These results indicated that TET exerted a regulatory effect on inflammatory reactions mediated by mast cells.