The <i>FTO</i> rs9939609 polymorphism is associated with short leukocyte telomere length in nonobese individuals

Yu, Ji Hee,Baik, Inkyung,Cho, Hyun Joo,Seo, Ji A.,Kim, Sin Gon,Choi, Kyung Mook,Baik, Sei Hyun,Choi, Dong Seop,Shin, Chol,Kim, Nan Hee Williams & Wilkins Co 2017 Medicine Vol.96 No.30

<▼1><P>Supplemental Digital Content is available in the text</P></▼1><▼2><P><B>Abstract</B></P><P>The fat mass and obesity-associated (FTO) rs9939609 polymorphism have been associated with the increased metabolic risk and mortality, irrespective of obesity. The mechanism underlying this association is not known. We aimed to evaluate whether the FTO rs9939609 risk variant is independently associated with metabolic risk factors and/or leukocyte telomere length (LTL). We further aimed to investigate whether this relationship is modified by obesity status.</P><P>A total of 2133 participants were recruited from the Korean Genome and Epidemiology Study. LTL was measured using the real-time quantitative polymerase chain reaction methodology. The <I>FTO</I> rs9939609 polymorphism was genotyped using DNA samples collected at baseline.</P><P>The proportions of the TT, TA, and AA genotypes were 76.7%, 21.5%, and 1.8%, respectively, and obese subjects comprised 44.5% of the total subjects. Among the 1184 nonobese subjects, body mass index, waist circumference, and visceral fat area were higher in subjects with the <I>FTO</I> risk allele than in noncarriers. In contrast, only high-sensitive C-reactive protein level was associated with the <I>FTO</I> risk allele in the obese subjects. LTL was significantly shorter in carriers of the <I>FTO</I> risk allele compared with noncarriers after controlling for several confounding factors (<I>P</I> < .01). Of particular note, this significant association between the <I>FTO</I> risk allele and LTL appeared only in nonobese subjects (<I>P</I> = .03). Multivariate linear regression analyses identified older age, low high-density lipoprotein cholesterol level, and the presence of the <I>FTO</I> risk allele as independent risk factors affecting LTL. This finding was evident only in nonobese subjects.</P><P>The <I>FTO</I> rs9939609 polymorphism is an independent risk factor for obesity and also for biological aging in the nonobese population.</P></▼2>

Evening Chronotype Is Associated With Metabolic Disorders and Body Composition in Middle-Aged Adults

Yu, Ji Hee,Yun, Chang-Ho,Ahn, Jae Hee,Suh, Sooyeon,Cho, Hyun Joo,Lee, Seung Ku,Yoo, Hye Jin,Seo, Ji A,Kim, Sin Gon,Choi, Kyung Mook,Baik, Sei Hyun,Choi, Dong Seop,Shin, Chol,Kim, Nan Hee Issued for the Endocrine Society by the Williams & 2015 The Journal of clinical endocrinology & metabolism Vol.100 No.4

Characterization of two novel non-reducing polyketide synthase genes from the lichen-forming fungus Hypogymnia physodes

Yu, Nan Hee,Kim, Jung A,Jeong, Min Hye,Cheong, Yong Hwa,Jung, Jae Sung,Hur, Jae-Seoun Springer-Verlag 2013 Mycological progress Vol.12 No.3

Differential circulating and visceral fat microRNA expression of non-obese and obese subjects

Kim, Nan Hee,Ahn, Jiyun,Choi, Yong Min,Son, Hyo Jung,Choi, Won Hee,Cho, Hyun Joo,Yu, Ji Hee,Seo, Ji A,Jang, Young Jin,Jung, Chang Hwa,Ha, Tae Youl Elsevier 2020 Clinical nutrition Vol.39 No.3

<P><B>Summary</B></P> <P><B>Background & aims</B></P> <P>Altered microRNA (miRNA) expression is associated with the pathophysiology of obesity; however, little is known about the miRNAs commonly dysregulated in the blood and visceral fat tissue of obese patients. This study compared the circulating and visceral fat miRNA expression in subjects with and without obesity.</P> <P><B>Methods</B></P> <P>For the circulating miRNA study, 20 healthy control and 30 obese subjects were recruited. For the tissue miRNA expression study, omental fat tissue was collected in ten female subjects each in the control and obese groups. MiRNA expression was measured by TaqMan low-density arrays. Metabolic risk factors were measured. Target genes for selected miRNAs were analyzed using informatics tools and a functional network map was constructed.</P> <P><B>Results</B></P> <P>11 miRNAs were down-regulated (miR-133a, -139-5p, -15b, -26a, -301, -30b, -30c, -374, -451, -570, and -636), and one was up-regulated (miR-155) in both depots in obese subjects. These miRNAs had significant associations with BMI, waist circumference, and fat mass. Among them, miR-15b, miR-26a, miR-301, miR-30b, and miR-30c had more predicted obesity-related target genes than other miRNAs. In particular, miR-15b had numerous target genes associated with adipogenesis, mammalian target of rapamycin (mTOR) signaling, diabetes and insulin resistance, and mitochondrial function.</P> <P><B>Conclusions</B></P> <P>It is suggested that the miRNA alteration in the serum and visceral fat has pathophysiological implications for obesity. Our study identified dysregulated miRNAs that may be novel therapeutic targets to combat obesity.</P>

Risk of the Development of Diabetes and Cardiovascular Disease in Metabolically Healthy Obese People : The Korean Genome and Epidemiology Study

Kim, Nan Hee,Seo, Ji A,Cho, Hyunjoo,Seo, Ji Hye,Yu, Ji Hee,Yoo, Hye Jin,Kim, Sin Gon,Choi, Kyung Mook,Baik, Sei Hyun,Choi, Dong Seop,Shin, Chol,Cho, Nam Han Williams & Wilkins Co 2016 Medicine Vol.95 No.15

<P><B>Abstract</B></P><P>The reported effects of a metabolically healthy obese (MHO) phenotype on diabetes and cardiovascular disease (CVD) risk are contradictory. Within the context of a population-based cohort study, we aimed to investigate the long-term risk of an MHO status for the development of diabetes and CVD, and whether consistency of this phenotype or age affected cardiometabolic outcomes.</P><P>We recruited 7588 subjects without diabetes or CVD, aged 40 to 69 years at baseline examination, from the Korean Genome and Epidemiology Study, and followed-up these subjects for 10 years biennially. Participants were divided into 4 groups based on the body mass index and the presence of metabolic syndrome: metabolically healthy normal weight (MHNW), MHO, metabolically unhealthy normal weight (MUNW), and metabolically unhealthy obese (MUO). We defined persistent phenotypes if subjects maintained the same phenotype at every visit from baseline to their last visit. Incident diabetes and CVD morbidity or mortality were identified during 10 years of follow-up.</P><P>Compared to MHNW controls, MUNW and MUO groups had increased risk for development of diabetes (hazard ratio [HR] 3.0 [95% CI: 2.5–3.6], and 4.0 [3.4–4.7], respectively) and CVD (HR 1.6 [1.3–2.0], and 1.9 [1.5–2.4], respectively). However, the MHO group showed only a marginal increase in risk for diabetes and CVD (HR 1.2 [0.99–1.6], 1.4 [0.99–1.8], respectively). The impact of MHO on the development of diabetes was more prominent in younger individuals (HR 1.9 [1.2–3.1] vs 1.1 [0.8–1.4], <45 years vs ≥45 years at baseline). Only 15.8% of MHO subjects maintained the MHO phenotype at every visit from baseline to the 5th biennial examination (persistent MHO). In subjects with persistent MHO, the risk for diabetes and CVD was significantly higher than those with persistent MHNW (1.9 [1.2–3.1], 2.1 [1.2–3.7], respectively).</P><P>MHO phenotype, even if maintained for a long time, was associated with a significantly higher risk for the development of diabetes and CVD in Korean subjects.</P>

Obstructive sleep apnea with excessive daytime sleepiness is associated with non-alcoholic fatty liver disease regardless of visceral fat

( Ji Hee Yu ),( Jae Hee Ahn ),( Hye Jin Yoo ),( Ji A Seo ),( Sin Gon Kim ),( Kyung Mook Choi ),( Sei Hyun Baik ),( Dong Seop Choi ),( Chol Shin ),( Nan Hee Kim ) 대한내과학회 2015 The Korean Journal of Internal Medicine Vol.30 No.6

Background/Aims: Obstructive sleep apnea (OSA) is associated with an increased risk of obesity and non-alcoholic fatty liver disease (NAFLD), but it remains unclear whether the risk of NAFLD is independently related to OSA regardless of visceral obesity. Thus, the aim of the present study was to examine whether OSA alone or in combination with excessive daytime sleepiness (EDS) or short sleep duration was associated with NAFLD independent of visceral fat in Korean adults. Methods: A total of 621 participants were selected from the Korean Genome and Epidemiology Study (KoGES). The abdominal visceral fat area (VFA) and hepatic fat components of the participants were assessed using computed tomography scans and they were then categorized into four groups depending on the presence of OSA and EDS. Results: The proportions of NAFLD were 21.1%, 18.5%, 32.4%, and 46.7% in participants without OSA/EDS, with only EDS, with only OSA, and with both OSA and EDS, respectively. A combination of OSA and EDS increased the odds ratio (OR) for developing NAFLD (OR, 2.75; 95% confidence interval [CI], 1.21 to 6.28) compared to those without OSA/EDS, and this association remained significant (OR, 2.38; 95% CI, 1.01 to 5.59) even after adjusting for VFA. In short sleepers (< 5 hours) with OSA, the adjusted OR for NAFLD was 2.50 (95% CI, 1.08 to 5.75) compared to those sleeping longer than 5 hours without OSA. Conclusions: In the present study, OSA was closely associated with NAFLD in Korean adults. This association was particularly strong in those with EDS or short sleep duration regardless of VFA.

Puccinia sp.에 의한 이팝나무 잎녹병 발생 및 중간기주 보고

유난희(Nan Hee Yu),박애란(Ae Ran Park),윤혁준(Hyeokjun Yoon),손연경(Youn Kyoung Son),이병희(Byoung-Hee Lee),김진철(Jin-Cheol Kim) 한국식물병리학회 2020 식물병연구 Vol.26 No.3

2018년 7월 전라남도 강진군의 가로수 전체 이팝나무에 심각한 녹병증상이 발견되어 잎을 채집하였다. 채집한 이팝나무 잎앞면에서 황색 및 갈색의 병반과 잎 뒷면에서 황색의 녹포자기가 관찰되었고, 현미경아래에서 41.2 μm 크기의 구형 녹포자와28.84 μm 크기의 구형 하포자가 확인되었다. 이팝나무 녹병균의 부분 small subunit rRNA, internal transcribed spacer (ITS) 1, 5.8S rRNA, ITS2, 부분 large subunit rRNA 염기서열을 이용하여 계통학적 유연관계를 분석한 결과, 대나무 녹병균으로 보고되어져 있는 Puccinia kusanoi와 가까운 근연관계를 가지고 있었다. 또한 2019년 5월 무위사로 이팝나무 주변에 식재된 대나무에서도 녹병 병징이 관찰되었고, 잎 뒷면에서 동포자퇴와 동포자를 확인하였으며, 대나무에서 채집한 동포자의 염기서열분석 결과, 이팝나무 녹병균과 100% 일치하였다. 본 논문은 대나무 녹병균으로 알려진 P. kusanoi와 근연관계가 가까운 새로운 종 Puccinia sp. JCK-KCFR1 strain (MT729824)가 국내에서 이팝나무와 대나무를 기주교대하며 녹병을 발생시키는 것을 처음으로 보고한다. In July 2018, a serious rust symptom was found throughout the fringe trees planted in Gangjin-gun, Korea. Yellow and brown spots were observed on the adaxial (topside) surface of the collected fringe tree leaves, and yellow color aecia were observed on the abaxial (underside) surface leaves. The size of aeciospore and urediniospores of JCK-KCFR1 strain were measured to 41.2 μm (Φ) and 28.84 μm (Φ) with a light microscope. Phylogenetic analysis of the small subunit rRNA, internal transcribed spacer, and large subunit rRNA region indicated that JCK-KCFR1 strain is novel species of the genus Puccinia and closely related to Puccinia kusanoi, which has been reported a rust pathogen on bamboo. In May 2019, rust symptoms were also discovered on the bamboo leaves planted around the fringe tree on Muwisa-ro, and their telia and teliospores were observed on the abaxial leaf surfaces of the bamboo with 100% sequence homology with the rust of the fringe tree. This is the first report that Puccinia sp. JCK-KCFR1 is a new species that requires both primary (fringe tree) and alternative (bamboo) host plants to complete its life cycle in Korea.

Speckle noise reduction on a laser projection display via a broadband green light source

Yu, Nan Ei,Choi, Ju Won,Kang, Heejong,Ko, Do-Kyeong,Fu, Shih-Hao,Liou, Jiun-Wei,Kung, Andy H.,Choi, Hee Joo,Kim, Byoung Joo,Cha, Myoungsik,Peng, Lung-Han The Optical Society 2014 Optics express Vol.22 No.3

Nifedipine과 Captopril이 흰쥐의 Cisplatin 신독성에 미치는 영향

유병희,하종식,김구자,경난호 이화여자대학교 생명과학연구소 1991 생명과학연구논문집 Vol.2 No.-

To investigate the effect of nifedipine, a calcium channel blocker and captopril, an angiotensin I converting enzyme inhibitor on cisplastin induced nephrotoxicity, we administered nifedipine, captopril and cisplatin to rats(Sprague-Daweley) and followed the changes in renal function, Na+, K+-ATPase activity, electrolyte concentration, renal tissue oxygen consumption and morphological changes. Results obtained were as followings. 1) As compared with control group, a marked weight loss was observed in cisplatin administered group, and the change was not prevented by the pretreatment with nifedipine or captopril. 2) Blood pressure was lowered in cisplatin, nifedipine and captopril groups at 5th day after administration of cisplatin. Blood pressure was recovered by preteatment with nifedipine or captopril at 8th day. 3) Urine flow increased drastically and urine osmolality follwing cisplatin treatment and these changes were not alliviated by the pretreatment with nifedipine or captopril. 4) In cisplatin treated animals, the urinary excretion of Na+and K+ were significantly reduced but the fractional excretions were increased. In this case, however, the nifedifine and captopril pretreatments attenuated the changes in fractional excretion. 5) In cisplatin treated animals, both the urinary excretion and fractional excretion of Mg^2+were increased drastically. These changes were not improved by nifedipine pretreatment, but were significantly attenuated by captopril. 6) In cisplatin treated animals, the glomerular filtration rate was reduced markedly and this was not corrected by pretreatment with nifedipine or captopril. 7) As compared with control group, Na+, K+-ATPase activities of the outer medullary tissues were decreased remarkably following cisplatin treatment. The decreaments were not changed by preteatment with nifedipine or captopril. 8) As compared with control group, the Na+ content of the outer medullary tissue was increased and K+ content was decreased in cisplatin treated animals. These changes were not affected by pretreatment with nifedipine or captopril. 9) Oxygen consumptions of renal tissues was decreased following cisplatin treatment, and this change was not reverted by pretreatment with nifedipine or captopril. 10) There were no changes in plasma ADH level among 4 groups, however, plasma renin activity was increased significantly following cisplatin treatment, and the increment was reduced slightly by pretreatment with nifedipine or captopril. 11) In electron micreoscopic study, a focal coagulative necrosis and mitochondrial swelling of proximal tubular cells were observed in cisplatin treated animals. These findings were persisted in animals pretreated with nifedipine or captopril. These results indicate 1) The cisplatin induced nephrotoxicity is due to an impairment of tubular reabsorption systems, particularly active Na+ transport mechanism in the ascending Henle's loop, an impairment of countercurrent multiplication system and necrosis of proximal tubule, and 2) these alteration can not be prevented by pretreatment with nifedipine or captopril.

Poster Session:PS 0187 ; Endocrinology : The Relationship Between Serum Estradiol Level and Bapwv in Postmenopausal Women

( Nan Hee Cho ),( Nam Keong Kim ),( Gyeong Im Yu ),( Dong Hoon Shin ),( Ho Chan Cho ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: The number of postmenopausal women is increasing with aging and cardiovascular disease (CVD) is more prevalent recently. There may be the protective effect of endogenous estrogen on CVD in spite of some limitations. In the present study, we investigate the relationship between serum estradiol level and brachial-ankle PWV (baPWV) as non-invasive indicator of CVD in postmenopausal women. Methods: Among We recruited 714 Korean postmenopausal women from (February 2012 to December 2013). Metabolic parameters, clinical characteristics, biochemical markers, serum estradiol, and baPWV were obtained in each subject. Statistical analysis was performed using SPSS version 20.0 (SPSS Inc., Chicago, IL, USA) Results: Among 714 subjects (Mean age 67.52±9.24), The mean serum estradiol score was 23.3 ± 34.33, with a range of 7 to 418. Age, SBP, uric acid, BUN and creatinine were found to be signifi cantly associated with a higher baPWV levels. Women with a higher estradiol level had a signifi cantly lower risk of having a higher baPWV levels (P < 0.05). Conclusions: Postmenopausal women with a higher serum estradiol level had a reduced baPWV levels independent of age and other coronary risk factors. This supports a higher level of serum estradiol may alleviate the calcifi ed-plaque burden of coronary arteries in postmenopausal women.