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대학생의 우울정도 : 간호학전공과 타전공대학생을 중심으로
김지연,류나은,이소라,이지희,정윤정,주지연,황인혜 이화여자대학교 간호과학대학 2012 이화간호학회지 Vol.- No.46
Purpose: This study was designed to investigate sample’s general characteristics and compare depression degree among sample’s general characteristics and students’ major(nursing vs. other major). Methods: The subjects consisted of 318 university students. Data was collected by self-reported questionnaires, which were constructed BDI score. Data was analyzed by the SPSS/PC WIN 19.0 program. Results: The depression of nursing students and other major students was not significantly different according to BDI score. Statistically significant difference was identified among sleeping. Conclusion: Specific study focused on the practice time should be done to confirm the depression of nursing major and other majors. Detailed support programs which specifically deal with sleeping should be developed to effectively reduce the harmful effects of individual vulnerability. Therefore, prevention and management system to reduce depression degree for university students is needed.
( Hyun Ju Lee ),( Ja Sung Rho ),( Shao Ran Gui ),( Mi Kyung Kim ),( Yu Kyoung Lee ),( Yeon Sook Lee ),( Jeong Eun Kim ),( Eu Na Cho ),( Mong Cho ),( Tae Ho Hwang ) 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3
Background/Aims: JX-594 is an oncolytic virus derived from the Wyeth vaccinia strain that causes replication-dependent cytolysis and antitumor immunity. Starting with a cross-examination of clinical-trial samples from advanced hepatocellular carcinoma patients having high levels of aldosterone and virus amplification in JX-594 treatment, we investigated the association between virus amplification and aldosterone in human cancer cell lines. Methods: Cell proliferation was determined by a cell-counting-kit-based colorimetric assay, and vaccinia virus quantitation was performed by quantitative polymerase chain reaction (qPCR) and a viral plaque assay. Also, the intracellular pH was measured using a pH-sensitive dye. Results: Simultaneous treatment with JX-594 and aldosterone significantly increased viral replication in A2780, PC-3, and HepG2 cell lines, but not in U2OS cell lines. Furthermore, the aldosterone treatment time altered the JX-594 replication according to the cell line. The JX-594 replication peaked after 48 and 24 hours of treatment in PC-3 and HepG2 cells, respectively. qPCR showed that JX-594 entry across the plasma membrane was increased, however, the changes are not significant by the treatment. This was inhibited by treatment with spironolactone (an aldosterone-receptor inhibitor). JX-594 entry was significantly decreased by treatment with EIPA [5-(N-ethyl-N-isopropyl)amiloride; a Na+/H+-exchange inhibitor], but aldosterone significantly restored JX-594 entry even in the presence of EIPA. Intracellular alkalization was observed after aldosterone treatment but was acidified by EIPA treatment. Conclusions: Aldosterone stimulates JX-594 amplification via increased virus entry by affecting the H+ gradient. (Korean J Hepatol 2011;17:213-219)
Hwang, Na Lam,Kang, Yong Jung,Sung, Bokyung,Hwang, Seong Yeon,Jang, Jung Yoon,Oh, Hye Jin,Ahn, Yu Ra,Kim, Do Hyun,Kim, Su Jeong,Ullah, Sultan,Hossain, Mohammad Akbar,Moon, Hyung Ryong,Chung, Hae Young Spandidos Publications 2017 Oncology Reports Vol.38 No.3
<P>Colorectal cancer (CRC) is the third most frequently diagnosed cancer and cause of cancer-related deaths. Despite advancements in conventional therapeutic approaches to CRC, most patients with CRC die of their disease. There is a need to develop novel therapeutic agents for this malignancy. Therefore, the present study aimed to examine the anticancer effects and elucidate the underlying mechanism of MHY451 in HCT116 human colorectal cancer cells. Treatment with MHY451 inhibited cell growth in a time- and concentration-dependent manner. MHY451 increased the accumulation of cell cycle progression at the G2/M phase. This agent decreased the protein level of cyclin B1 and its activating partners, Cdc25c and Cdc2, whereas it increased the cell cycle inhibitor p21WAF/CIP. The induction of apoptosis was observed by decreased viability, cleavage of poly(ADP-ribose) polymerase (PARP), alteration in the ratio of Bax/Bcl-2 protein expression and reduction of procaspase-8 and -9. Pretreatment with Z-VAD-FMK, a pan-caspase inhibitor, inhibited MHY451-induced apoptosis, indicating that apoptotic cell death by MHY451 was mediated through caspases. Moreover, the apoptotic effect of MHY451 was reactive oxygen species (ROS)-dependent, evidenced by the inhibition of MHY451-induced PARP cleavage and ROS generation by N-acetylcysteine-induced ROS scavenging. Taken together, these results demonstrate that MHY451 exerts anticancer effects by regulating the cell cycle, inducing apoptosis through caspase activation and generating ROS. These results suggest that MHY451 has considerable potential for chemoprevention or treatment of CRC or both.</P>
Kim, Seong-Yeon,Na, Yeon-Joo,Kim, Dong-Ju,Kim, Yeong-Seok,Kim, Hyeong-Min,Hwang, Sung-Ha,Kwak, Ji-Yeon,Kuh, Hyo-Jeong,Lee, Jae-Hwi The Korean Society of Pharmacology 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.3
The objective of the present study was to establish the method of measurement of hydrogen peroxide and to estimate the anti-oxidative effect of genistein in the skin. UVB induced skin oxidation and anti-oxidative effect of genistein formulations were evaluated by determining levels of hydrogen peroxide. The mechanism involved in the determination of hydrogen peroxide is based on a color reaction between ferric ion ($Fe^{3+}$) and xylenol orange, often called FOX assay and subsequent monitoring of absorbance values of the reactant at 540 nm. The reaction was to some extent pH-dependent and detection sensitivity was greatest at pH 1.75. Genistein liposomal gel demonstrated better anti-oxidative effect with regard to lowering hydrogen peroxide levels elevated by UVB irradiation compared to genistein-suspended gel. A linear relationship has been observed between anti-oxidative effect of genistein and drug deposition in the skin tissue. Genistein liposomal gel resulting in the localization of the drug in the deeper skin led to improved anti-oxidative effect compared to genistein gel. The suggested method for evaluation of oxidation of the skin can be used as a tool to screen effective anti-oxidative agents and their delivery systems acting on the skin.
Kim, Seung Up,Seo, Yeon Seok,Lee, Han Ah,Kim, Mi Na,Lee, Yu Rim,Lee, Hye Won,Park, Jun Yong,Kim, Do Young,Ahn, Sang Hoon,Han, Kwang-Hyub,Hwang, Seong Gyu,Rim, Kyu Sung,Um, Soon Ho,Tak, Won Young,Kweon Elsevier 2019 Journal of hepatology Vol.71 No.3
<P><B>Background & Aims</B></P> <P>It is currently unclear which antiviral agent, entecavir (ETV) or tenofovir disoproxil fumarate (TDF), is superior for improving prognosis in patients with chronic hepatitis B (CHB). Here, we assessed the ability of these 2 antivirals to prevent liver-disease progression in treatment-naïve patients with CHB.</P> <P><B>Methods</B></P> <P>From 2012 to 2014, treatment-naïve patients with CHB who received ETV or TDF as a first-line antiviral agent were recruited from 4 academic teaching hospitals. Patients with decompensated cirrhosis or hepatocellular carcinoma (HCC) at enrollment were excluded. Cumulative probabilities of HCC and death or orthotopic liver transplant (OLT) were assessed.</P> <P><B>Results</B></P> <P>In total, 2,897 patients (1,484 and 1,413 in the ETV and TDF groups, respectively) were recruited. The annual HCC incidence was not statistically different between the ETV and TDF groups (1.92 <I>vs</I>. 1.69 per 100 person-years [PY], respectively; adjusted hazard ratio [HR] 0.975 [<I>p</I> = 0.852] by multivariate analysis). Propensity score (PS)-matched and inverse probability of treatment weighting (ITPW) analyses yielded similar patterns of results (HR 1.021 [<I>p</I> = 0.884] and 0.998 [<I>p</I> = 0.988], respectively). The annual incidence of death or OLT was not statistically different between the ETV and TDF groups (0.52 <I>vs</I>. 0.53 per 100 PY, respectively; adjusted HR 1.202 [<I>p</I> = 0.451]). PS-matched and ITPW analyses yielded similar patterns of results (HR 1.248 [<I>p</I> = 0.385] and 1.239 [<I>p</I> = 0.360], respectively). These findings were consistently reproduced in patients with compensated cirrhosis (all <I>p</I> >0.05).</P> <P><B>Conclusions</B></P> <P>The overall prognosis in terms of HCC and death or OLT was not statistically different between the ETV and TDF groups. Further studies are needed to validate our results.</P> <P><B>Lay summary</B></P> <P>It is currently unclear which antiviral agent, entecavir or tenofovir disoproxil fumarate, is superior for improving prognosis in patients with chronic hepatitis B virus infection. In this analysis we found that there was no difference in terms of overall prognosis, including risk of hepatocellular carcinoma, death, or the need for a liver transplant, in patients receiving either antiviral.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The hepatocellular carcinoma risk was not statistically different between the ETV and TDF groups. </LI> <LI> The death or liver transplant risk was not statistically different between the 2 groups. </LI> <LI> These results were consistently reproduced after adjusting for confounding variables. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>