대한약전 수재 의약품의 기기분석법에 관한 연구(ⅩⅡ) : 고속액체크로마토그래프를 이용한 아크리놀 및 그 제제의 분석법에 관한 연구 Studies on analytical Methods of actinol and its preparations by HPLC

이석호,옥치석,최보경,김영옥,최리나,박상애,최명신,김길수,이강춘 식품의약품안전청 1997 식품의약품안전청 연보 Vol.1 No.-

아크리놀은 살균,소독에 쓰이는 약물로서 연로제 및 지사제용도의 경구투여제제등게 이용되고 있다. 공정서에 수재된 아크리놀의 분석법은 적정법이고 제제에서늬 분석법은 확립되지 않았고 원료의 적정법을 제제에 적용시 다른 주성분이나 부형제 또는 안정제 드으이 영향으로 분석오차가 생길 우려가 있다. 따라서 아크리놀을 보다 신속·정확하게 확인 및 정량분씬을 할 수 있고 강도와 분리능이 우수한 HPLC를 이용하여 아크리놀을 분석하고자 하였다. MPLC 분석조건은 이동상 :아세료니트릴 · 1% 인산일수소나트륨혼합액(40:60)(p템 5.2), 칼랏 :옥타데실실릴화한 실리카칼럼,검출기 :자외부흡광광도계(측정파장 : 254nm)을 사용하여 양호한 결과를 얻었다. 틴PLC법의 분석 결과, 검량선의 상관계수는 0.9999, 재현성(aSD(%))은 0.5234. 정균회수율은 100.28%(RSD=0.89%)로 정량성 및 재현성이 높온 분석 결과를·얻었다. 또한 시판품에 대하여 려PLC분석법과 공정서에 수재된 적정법의 시칩결과를 비교하였을 때 핀Plf법인 더욱 정확한 결과를 얻을 수 있었으므로, 조작이 간편하고 재현성이 높은 딘PLC분석법을 약전차정의 기초자료로 활용하고자 한다. We describe a method for the determination of acrino1 and its preparations by HPLC. The optimum analrtical coriditions were as follows;column : u-Bondapak C_(18) mobile phase : acetonitrile 1% Na_(2)HPO_(4), buffer (40:60)(pH 5.2), detector : 254nm, flow rate 1.OmL/min, injection volumn : 5uL, The calibration curve at 254nm was linear within the range of 10 ~ 140㎍/mL. This HPLC method has been validated to be precise, accurate and linear. This method has also been found to be applicable to other formulations containing acrinol.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

체성 연부 조직에 생긴 평활근육종: 1예 보고

이경지 ( Kyung Ji Lee ),이안희 ( An Hi Lee1 ),김진아 ( Jea Na Kim ),김형민 ( Hyoung Min Kim ),이교영 ( Kyo Young Lee ) 대한임상종양학회 2009 Korean Journal of Clinical Oncology Vol.5 No.2

체성 연부 조직에 생기는 평활근육종은 매우 드물다. 66세 남자 환자가 방사선 소견상 경계가 좋은 양성 종양이 의심되어 절제술을 시행 받았다. 육안조직소견상 경계가 좋은 둥근 종괴이나, 현미경 소견에서 세포충실도가 증가되고 부분적으로 중증도의 핵이형성을 보였다. 또한 면역조직화학염색상 Smooth muscle actin과 Desmin에 동시에 양성이어 평활근세포 기원으로 생각되었다. 저자들은 괴사가 없고 유사분열은 단 한 개만이 관찰되었으나 중증도의 핵이형성을 보였기에 평활근육종으로 진단한 증례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Leiomyosarcoma of somatic soft tissue is a rare tumor compared with retroperitoneal lesion. We report a case of a leiomyosarcoma of the somatic soft tissue in a 66-year-old man. He presented as an enlarging mass in the left thigh for eight months. Radiologic examinations revealed a well defined round mass, suspicious of benign tumor, such as hemangioma or leiomyoma. He underwent surgical resection. The mass was 3.0 cm in diameter, and it was confined within adductor longus muscle without any connection to adjacent neurovascular bundles. Histologic examination showed moderately cellularity and focal marked atypia with a fascicular growth pattern of spindle cells showing blunt ended nuclei. In addition, the proliferation index was 2~3% by immunohistochemistry using monoclonal antibody MIB-1. But only one definite microscopic mitotic feature was found. Although it showed low mitotic activity without necrosis, this case was diagnosed leiomyosarcoma according to marked cellular atypism.

Original Articles : Effect of aldosterone on the amplification of oncolytic vaccinia virus in human cancer Lines

( Hyun Ju Lee ),( Ja Sung Rho ),( Shao Ran Gui ),( Mi Kyung Kim ),( Yu Kyoung Lee ),( Yeon Sook Lee ),( Jeong Eun Kim ),( Eu Na Cho ),( Mong Cho ),( Tae Ho Hwang ) 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3

Background/Aims: JX-594 is an oncolytic virus derived from the Wyeth vaccinia strain that causes replication-dependent cytolysis and antitumor immunity. Starting with a cross-examination of clinical-trial samples from advanced hepatocellular carcinoma patients having high levels of aldosterone and virus amplification in JX-594 treatment, we investigated the association between virus amplification and aldosterone in human cancer cell lines. Methods: Cell proliferation was determined by a cell-counting-kit-based colorimetric assay, and vaccinia virus quantitation was performed by quantitative polymerase chain reaction (qPCR) and a viral plaque assay. Also, the intracellular pH was measured using a pH-sensitive dye. Results: Simultaneous treatment with JX-594 and aldosterone significantly increased viral replication in A2780, PC-3, and HepG2 cell lines, but not in U2OS cell lines. Furthermore, the aldosterone treatment time altered the JX-594 replication according to the cell line. The JX-594 replication peaked after 48 and 24 hours of treatment in PC-3 and HepG2 cells, respectively. qPCR showed that JX-594 entry across the plasma membrane was increased, however, the changes are not significant by the treatment. This was inhibited by treatment with spironolactone (an aldosterone-receptor inhibitor). JX-594 entry was significantly decreased by treatment with EIPA [5-(N-ethyl-N-isopropyl)amiloride; a Na+/H+-exchange inhibitor], but aldosterone significantly restored JX-594 entry even in the presence of EIPA. Intracellular alkalization was observed after aldosterone treatment but was acidified by EIPA treatment. Conclusions: Aldosterone stimulates JX-594 amplification via increased virus entry by affecting the H+ gradient. (Korean J Hepatol 2011;17:213-219)

De novo Hyponatremia in Patients Undergoing Peritoneal Dialysis: A 12-month Observational Study

( Hyun Hee Lee ),( Soo Jung Choi ),( Heon Nam Lee ),( Sun Young Na ),( Jae Hyun Chang ),( Woo Kyung Chung ),( Se Joong Kim ) 대한신장학회 2010 Kidney Research and Clinical Practice Vol.29 No.1

Purpose: Hyponatremia occurs infrequently in patients undergoing peritoneal dialysis (PD). Nevertheless, one must understand its pathophysiology, since the therapeutic strategy differs from that of non-PD-related hyponatremia. This study examined the clinical features of hyponatremia in PD and evaluated the factors that may contribute to its development. Methods: We retrospectively enrolled 51 normonatremic PD patients at Gachon University Gil Hospital, South Korea. Using the plasma sodium levels at month 13, the patients were divided into hyponatremia (Na+<135mEq/L) and normonatremia (Na+≥135mEq/L) groups. Then, the clinical variables of these patients were examined, including peritoneal function and adequacy tests, and biochemical parameters. Results: The de novo hyponatremia (n=8) and normonatremia (n=43) groups had no significant differences in baseline characteristics. At month 1, the serum albumin was lower in the hyponatremia group (p=0.022). In the peritoneal equilibration test analysis, the dialysate-to-plasma ratio for creatinine (D/Pcr) measured after 13 months differed significantly between the two groups (p=0.007), while the maximum dip in sodium did not differ. No significant differences were observed in the normalized protein equivalent of nitrogen appearance, Kt/V, or residual renal function. Conclusion: Our data suggest that the development of hyponatremia is associated with a lower initial serum albumin level and increased D/Pcr in patients undergoing PD. Therefore, the serum sodium levels should be monitored more carefully in these patients.

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.

한열변증에 따른 중풍 환자의 제반특성 비교

김민경,이인환,신애숙,김나희,김혜미,심소라,나병조,조승연,박성욱,정우상,문상관,박정미,고창남,조기호,김영석,배형섭,Kim, Min-kyung,Lee, ln-whan,Shin, Ae-sook,Kim, Na-hee,Kim, Hye-mi,Shim, So-ra,Na, Byung-Jo,Cho, Seung-yeon,Park, Seong-uk,Jung, Woo Sang 대한중풍순환신경학회 2010 대한중풍.순환신경학회지 Vol.11 No.1

Object : This study was conducted as part of the national project to standardize stroke diagnosis in Korean medicine. In this study, we aimed to evaluate the characteristics of acute stroke between heat pattern group and cold pattern group. Methods : We recruited stroke patients from 5 universities(Kyung-Hee University oriental medical center, Kyung-Hee University East-West Neo medical center, Kyungwon university lncheon oriental medical center, Kyungwon university Songpa oriental medical center and DongGuk university llsan oriental medical center) from April, 2007 until February, 2010. We diagnosed them and selected 463 heat pattern patients and 182 cold pattern patients. Results : We find that the risk factor of smoking, alcohol, diet(prefer to meat) are more associated with the heat pattern group. On the other hand, inflammation history in recent 3 months and diet(prefer to sea food) are more associated with the cold pattern group. RBC, Hg, Hct, TG, CK and Cl are more related to the heat pattern group. CPT, total cholesterol and HDL are more related to the cold pattern group. Tae-eum type takes high distribution of the heat pattern group, and So-eum type takes high distribution of the cold pattern group. Conclusions : This study provides evidence that the heat pattern group have more risk factor than the cold pattern group.

비가림 및 망사 시설재배가 참다래의 수체생육 및 과실품질에 미치는 영향

나양기,마경철,임동근,김병삼,임경호,김월수,이상현,박용서 全南大學校 農業科學技術硏究所 2001 農業科學技術硏究 Vol.36 No.-

This studies were conducted to investigate the effects of rain shelter and netting on the tree growth, fruit set fruit quality of kiwifruit (Actinidia deliciosa). In rain shelter and net house, shoot falling wasn't occurred by strong wind, and fruit set, fruit weight and also fruit malformation, side flowering increased at the same time. In rain shelter house the increment temperature forced blooming date and increased shoot length, leaf number and diameter but in net house these tendencies were not observed. Relative light intensity of rain shelter house was higher than that of net house with standard of field culture (relative light intensity: 100%). Soluble solid content was higher in rain shelter house, and acid content was higher in net house. With the effect of rain sheltering the occurrence of bacterial bloom rot and canker significantly decreased in both rain shelter and net house but insect injury increased. These data showed that rain shelter house is more suitable for kiwi fruit culture.

e-pedigree를 이용한 스마트 의약품 콜드체인 물류허브시스템의 설계

남나경·이기한 서울여자대학교 컴퓨터과학연구소 2014 정보기술논문지 Vol.12 No.-

대학생의 라이프스타일 유형에 따른 신용카드 사용과 의복구매

나영주,이은희,장경자 한국의류산업학회 2004 한국의류산업학회지 Vol.6 No.5

This study aimed to classify the lifestyles of 1020 college students, and to analyze the effect of the lifestyles on the usages of credit card, price perception, purchasing satisfaction and the number of new clothing. The number of credit cards, total payment of credit cards and the attitude to credit card were different by the 7 clusters of college students, but the frequency of credit card use, the amount of cash service and arrearages were not different. The perception to the apparel price, purchasing satisfaction, and the degree of clothing purchasing varied according to the lifestyle clusters. For example, 6th cluster being highest in the pursuit of appearance showed the highest amount of credit cards usage significantly and tendency of highest arrearage, and used credit cards mostly in clothing purchase.